FastQCFastQC Report
Thu 26 May 2016
SRR937513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478555
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.8596713021491783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30100.6289768156220288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.4507318907962512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9580.2001859765335228No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9560.19976805173908957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.15400528674864958No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7210.15066188839318365No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6790.14188546771008556No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5670.11848167922182402No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.1071977097721265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42250.035.1150471
GGTATCA26250.032.787981
GGCCGTT650.005845574729.2257777
ATCAACG52050.028.3060193
TATCAAC52300.028.2645452
TCAACGC52950.027.911734
CAACGCA53250.027.8437235
AACGCAG54850.027.1905426
GATGTGC2701.8189894E-1224.6288
ACGCAGA60950.024.310867
GTACATG30600.024.2420081
CGCAGAG61150.024.1562128
GGTGTGC5150.023.9789988
GGACCGA1000.001652871523.7484286
AAATCGA701.7807055E-523.74718580-81
GAGTACT30400.023.43349612-13
TACATGG31350.023.3489042
ATGTGCA3300.023.028789
ACATGGG31500.022.9335293
AGTACTT31200.022.90874712-13