FastQCFastQC Report
Thu 26 May 2016
SRR937513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478555
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49731.0391700013582557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.8561189413964956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30130.6296037028136787No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13560.2833530106257379No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10810.22588835139116717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.19893220215022306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.1709312409231959No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6270.13101942305482128No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.11033214573037582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35500.030.1295591
GTATCAA51400.028.7631131
GTACACG700.00835432327.1644291
GTAAGGT1858.923962E-925.679974
GGGCGTA1151.2796809E-424.7841639
TATCAAC60800.024.300942
ATCAACG61150.024.0841583
TCAACGC62300.023.6395854
CAACGCA62550.023.469155
ATGGGTA2452.9467628E-1023.2691975
AACGCAG64050.022.9147346
GAGTACT36950.022.81942712-13
TAAGGTG2303.607056E-922.721195
TATACTC1050.002192230722.622835
GGTGTGC4450.022.4171338
GTACTTT37800.022.30396314-15
GGTAAGG2154.4001354E-822.0967163
TGCACCG1302.9347165E-421.9267435
GCACCGT1302.9388675E-421.9221616
CGTCTAT1754.042544E-621.7133776
Produced by FastQC (version 0.11.5)