##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937513_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478555 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02815559340097 31.0 31.0 34.0 30.0 34.0 2 32.23864341611727 33.0 31.0 34.0 30.0 34.0 3 32.28767853224812 34.0 31.0 34.0 30.0 34.0 4 35.96132732914712 37.0 35.0 37.0 35.0 37.0 5 35.83697171693954 37.0 35.0 37.0 35.0 37.0 6 35.928737553677216 37.0 35.0 37.0 35.0 37.0 7 35.87911943245813 37.0 35.0 37.0 35.0 37.0 8 35.85988862304228 37.0 35.0 37.0 35.0 37.0 9 37.523254380374254 39.0 37.0 39.0 35.0 39.0 10-11 37.44631338090711 39.0 37.0 39.0 34.0 39.0 12-13 37.348196132106025 39.0 37.0 39.0 34.0 39.0 14-15 38.456729111596374 40.0 38.0 41.0 34.0 41.0 16-17 38.44866107343984 40.0 38.0 41.0 34.0 41.0 18-19 38.50855283091808 40.0 38.0 41.0 34.0 41.0 20-21 38.43604496870788 40.0 38.0 41.0 34.0 41.0 22-23 38.302671584248415 40.0 38.0 41.0 34.0 41.0 24-25 38.147440733040085 40.0 37.5 41.0 33.0 41.0 26-27 38.03226588375422 40.0 37.0 41.0 33.0 41.0 28-29 37.90198096352562 40.0 37.0 41.0 33.0 41.0 30-31 37.887095527159886 40.0 37.0 41.0 33.0 41.0 32-33 37.71676296350472 40.0 36.5 41.0 33.0 41.0 34-35 37.54464795060129 40.0 36.0 41.0 33.0 41.0 36-37 37.321056095955534 40.0 36.0 41.0 33.0 41.0 38-39 37.13943538360272 40.0 35.0 41.0 32.0 41.0 40-41 36.91088694089498 39.0 35.0 41.0 31.0 41.0 42-43 36.61040737219338 39.0 35.0 41.0 31.0 41.0 44-45 36.15809468086218 39.0 35.0 41.0 30.0 41.0 46-47 35.59881831764374 39.0 35.0 41.0 28.0 41.0 48-49 35.118854677100856 39.0 35.0 40.5 26.0 41.0 50-51 34.81943245812916 39.0 35.0 40.0 24.0 41.0 52-53 34.55846036505731 38.0 34.0 40.0 23.5 41.0 54-55 34.29062072280094 38.0 34.0 40.0 23.0 41.0 56-57 34.006393204542846 38.0 34.0 40.0 22.0 41.0 58-59 33.714555275778125 38.0 33.5 40.0 20.5 41.0 60-61 33.34404091483738 37.0 33.0 40.0 19.5 41.0 62-63 32.96164704161486 36.5 33.0 40.0 17.5 41.0 64-65 32.589150672336515 36.0 32.0 39.0 14.0 41.0 66-67 32.15089488146608 35.5 32.0 39.0 9.5 40.0 68-69 31.712954623815445 35.0 31.0 38.5 7.0 40.0 70-71 31.275381095171923 35.0 31.0 38.0 4.0 40.0 72-73 30.835574803314145 35.0 31.0 37.0 2.0 39.5 74-75 30.387330609856754 35.0 30.0 36.5 2.0 39.0 76-77 29.08416378472694 33.5 28.5 35.0 2.0 37.5 78-79 29.710756339396724 34.0 30.0 36.0 2.0 38.0 80-81 29.560344161068215 34.0 30.0 35.0 2.0 37.0 82-83 29.23274649726782 34.0 29.5 35.0 2.0 37.0 84-85 28.85727241382913 34.0 29.0 35.0 2.0 36.0 86-87 28.472559057997515 34.0 29.0 35.0 2.0 36.0 88-89 28.069723438267282 34.0 28.5 35.0 2.0 35.5 90-91 27.598788018096144 33.5 27.0 35.0 2.0 35.0 92-93 27.205993041552173 33.0 26.5 35.0 2.0 35.0 94-95 26.751017124468454 33.0 25.5 35.0 2.0 35.0 96-97 26.23189497549916 33.0 25.0 35.0 2.0 35.0 98-99 25.72066220183678 33.0 23.5 35.0 2.0 35.0 100-101 23.938300717785836 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07513792732628488 1101 2 0.1786910663625747 1101 3 0.06540417546146671 1101 4 0.08543423917660675 1101 5 -0.1342205735213895 1101 6 -0.02624537471761812 1101 7 0.09782828146768452 1101 8 0.02516901697176621 1101 9 0.21332291349462906 1101 10-11 0.028223866439212486 1101 12-13 0.02510885012170405 1101 14-15 0.02414586285778597 1101 16-17 0.10515959178468393 1101 18-19 0.04075793681618478 1101 20-21 -0.024101104311277766 1101 22-23 -0.041211614254898166 1101 24-25 0.039898214756625805 1101 26-27 -0.04368324981867744 1101 28-29 0.12694235698796064 1101 30-31 0.11007985544370058 1101 32-33 0.07954433323158838 1101 34-35 0.09226316842593008 1101 36-37 0.013241044899324095 1101 38-39 0.05259563807540246 1101 40-41 0.02997539701667762 1101 42-43 0.2831402635935234 1101 44-45 0.3182668285279888 1101 46-47 0.380750898247733 1101 48-49 0.4077310465809987 1101 50-51 0.13540948104514428 1101 52-53 0.31576379815351885 1101 54-55 0.25849474556125784 1101 56-57 0.18664403181849565 1101 58-59 -0.06957799471354775 1101 60-61 0.12262647629884071 1101 62-63 0.18779917074235186 1101 64-65 0.06605829039854427 1101 66-67 0.16737160475260993 1101 68-69 0.2853657464431407 1101 70-71 0.22019492573834754 1101 72-73 0.11182705436828044 1101 74-75 0.037326768999193405 1101 76-77 0.21017615538208645 1101 78-79 0.1462050146188325 1101 80-81 0.08778115501912964 1101 82-83 0.25364149555585414 1101 84-85 0.23428871149434727 1101 86-87 0.32641476440217687 1101 88-89 0.35666251024712636 1101 90-91 0.38162151839449976 1101 92-93 0.3470985258360173 1101 94-95 0.019783606341757576 1101 96-97 0.061877897480396626 1101 98-99 0.454635946642842 1101 100-101 0.6176942913742245 1102 1 0.16606190393447307 1102 2 0.21501075447174145 1102 3 0.16973166084157754 1102 4 0.047508313250681056 1102 5 -0.09634922849215144 1102 6 0.02524780267029314 1102 7 -0.0162769816902113 1102 8 0.10463880254681612 1102 9 0.06668743591178128 1102 10-11 0.18801723875890275 1102 12-13 0.13662152068700806 1102 14-15 0.2192296835205454 1102 16-17 0.07895296410436714 1102 18-19 -0.06562412946060903 1102 20-21 0.11504899315460904 1102 22-23 0.12606713818175308 1102 24-25 0.03552784438625878 1102 26-27 0.25561889443083174 1102 28-29 -0.022854913967201185 1102 30-31 0.21752235057040537 1102 32-33 0.1452714287481527 1102 34-35 0.3815614140399646 1102 36-37 0.3346289591293399 1102 38-39 0.11691922482003747 1102 40-41 0.44882529955078354 1102 42-43 0.5072689185642858 1102 44-45 0.5765670234597664 1102 46-47 0.5899165902555339 1102 48-49 0.9970487853724208 1102 50-51 1.003861722760547 1102 52-53 0.8878807572178502 1102 54-55 0.8977228157366994 1102 56-57 0.9466128427346732 1102 58-59 0.837657872732855 1102 60-61 0.8547122462793411 1102 62-63 0.7201851356546314 1102 64-65 0.895068036987233 1102 66-67 0.9052936320430547 1102 68-69 0.975950541764778 1102 70-71 0.8257972649196326 1102 72-73 0.9012383604111669 1102 74-75 1.152145482449484 1102 76-77 1.0755874614249699 1102 78-79 1.2279866910320898 1102 80-81 1.3570521101848207 1102 82-83 1.3590138152439657 1102 84-85 1.0936220448276792 1102 86-87 1.0805551152793704 1102 88-89 1.0864246934829929 1102 90-91 1.2325844813574598 1102 92-93 1.401114120377926 1102 94-95 1.0767114815854235 1102 96-97 0.9703847200924862 1102 98-99 0.8359263949859219 1102 100-101 1.0987664161305588 1103 1 -0.12335444740138612 1103 2 -0.061448367188624786 1103 3 -0.056905192730255294 1103 4 -0.05706685014801138 1103 5 -0.2784384384451357 1103 6 -0.07267674726664097 1103 7 -0.15129590153885175 1103 8 -0.19395080873628956 1103 9 -0.1784679361785777 1103 10-11 -0.27497439064139684 1103 12-13 -0.19241184704605274 1103 14-15 -0.18390642472392216 1103 16-17 -0.12205609448982813 1103 18-19 -0.06682811982869197 1103 20-21 -0.31067626770997236 1103 22-23 -0.20961684301306605 1103 24-25 -0.2830996065941278 1103 26-27 -0.12014076615854918 1103 28-29 -0.3663648325545239 1103 30-31 -0.32026001383734126 1103 32-33 -0.32127483888170616 1103 34-35 -0.12586319322984707 1103 36-37 -0.23600514246451354 1103 38-39 -0.18800566911414762 1103 40-41 -0.023052925423414194 1103 42-43 0.26397686490071237 1103 44-45 0.27250212264564055 1103 46-47 0.12767028822594995 1103 48-49 0.44424520780103904 1103 50-51 0.5498713546834892 1103 52-53 0.5654980029465477 1103 54-55 0.3781723054741164 1103 56-57 0.3747747507727368 1103 58-59 0.17838714689865043 1103 60-61 0.18761558305265424 1103 62-63 0.2833372971040049 1103 64-65 0.3486857413789366 1103 66-67 0.6527136526828912 1103 68-69 0.601509537292813 1103 70-71 0.4798506991588276 1103 72-73 0.6875874029521576 1103 74-75 0.6612614095220692 1103 76-77 0.6805639549463542 1103 78-79 0.7703270189761326 1103 80-81 0.9154456430365592 1103 82-83 0.9850314737693644 1103 84-85 0.7368682321915152 1103 86-87 0.7594910171254874 1103 88-89 0.7678259089399369 1103 90-91 0.571364437784478 1103 92-93 0.6499961293218561 1103 94-95 0.5110080072132206 1103 96-97 0.5726665685043635 1103 98-99 0.8876119815012338 1103 100-101 0.9929012630517846 1104 1 -0.11486599246868323 1104 2 -0.022980575428469763 1104 3 -0.03118560291935779 1104 4 -0.0104342969307325 1104 5 -0.05391784474105066 1104 6 -0.09616004379646625 1104 7 -0.2791016145069918 1104 8 -0.1332678894054382 1104 9 -0.2538840818317709 1104 10-11 -0.1882718368624623 1104 12-13 -0.08345499730781825 1104 14-15 -0.2460866111358868 1104 16-17 -0.23443094040286638 1104 18-19 -0.30879490503516394 1104 20-21 -0.4765292665304841 1104 22-23 -0.41692034333675565 1104 24-25 -0.4393829551514088 1104 26-27 -0.24361209046393384 1104 28-29 -0.2452325261719963 1104 30-31 -0.35951339044805053 1104 32-33 -0.2837470884479032 1104 34-35 -0.2609616167391522 1104 36-37 -0.22561739019791816 1104 38-39 -0.3857779485369903 1104 40-41 -0.1207980086821081 1104 42-43 0.010405151276323465 1104 44-45 -0.11819811945934333 1104 46-47 -0.08238312858778585 1104 48-49 0.08481833739962497 1104 50-51 -0.47270811280378666 1104 52-53 -0.4586918026304403 1104 54-55 -0.3176119934708552 1104 56-57 -0.2292727479191825 1104 58-59 -0.41138532171490283 1104 60-61 -0.21372811438410366 1104 62-63 -0.2546516056785748 1104 64-65 -0.3943348951886705 1104 66-67 -0.3116327673263868 1104 68-69 -0.05508050713744339 1104 70-71 -0.09893910109716941 1104 72-73 0.05970704833782747 1104 74-75 -0.005394171751600396 1104 76-77 -0.15415431348569442 1104 78-79 0.04567192250365437 1104 80-81 0.1807671342140651 1104 82-83 0.12036786371506025 1104 84-85 0.2503007724019284 1104 86-87 0.028266716761589095 1104 88-89 -0.14949036175649155 1104 90-91 -0.09409022591426108 1104 92-93 0.10842221044328326 1104 94-95 0.08750017501355245 1104 96-97 0.10227048002223427 1104 98-99 0.24233864527091598 1104 100-101 0.3729543546939915 1105 1 -0.24521727827301731 1105 2 -0.1553404297004164 1105 3 -0.03895277991911428 1105 4 -0.06112394105086594 1105 5 -0.2519301098293525 1105 6 -0.27683505100808503 1105 7 -0.1617045269312669 1105 8 -0.27583885704397915 1105 9 -0.15234987740651462 1105 10-11 -0.22311996558178038 1105 12-13 -0.39493401951959584 1105 14-15 -0.2024656856986482 1105 16-17 -0.4108150363868006 1105 18-19 -0.2590574612590544 1105 20-21 -0.3779077534801374 1105 22-23 -0.3970042651735355 1105 24-25 -0.04707641341484958 1105 26-27 -0.20294659190091835 1105 28-29 -0.5434915887688234 1105 30-31 -0.3198868987120278 1105 32-33 -0.38915820395126843 1105 34-35 -0.4033536294743385 1105 36-37 -0.4073915010324356 1105 38-39 -0.5515355955203987 1105 40-41 -0.693607280148683 1105 42-43 -0.6419402263452341 1105 44-45 -0.38627122921479184 1105 46-47 -0.7134555759429944 1105 48-49 -0.4667046489570623 1105 50-51 -0.2877742459889703 1105 52-53 -0.35619070753326554 1105 54-55 -0.19455512320514146 1105 56-57 -0.1944767030896415 1105 58-59 -0.5896724829025217 1105 60-61 -0.40140239509223363 1105 62-63 -0.45469514246849485 1105 64-65 -0.5494473963906081 1105 66-67 -0.39728806278894524 1105 68-69 -0.45995796309316717 1105 70-71 -0.15127139097206665 1105 72-73 0.038137640545006235 1105 74-75 -0.16845013388794783 1105 76-77 -0.1311265567788915 1105 78-79 0.04329075390142734 1105 80-81 -0.09704299196249977 1105 82-83 -0.1397390818719657 1105 84-85 -0.18962379944178664 1105 86-87 -0.22805680165206965 1105 88-89 -0.3394897207240035 1105 90-91 -0.20041347723104863 1105 92-93 -0.4567641163337086 1105 94-95 -0.3921857724858846 1105 96-97 -0.25880976656159405 1105 98-99 -0.12454428082871871 1105 100-101 0.22787801840834376 1106 1 -0.3676374523699586 1106 2 -0.11654154754629786 1106 3 -0.07685002118122242 1106 4 -0.08332035714633435 1106 5 -0.1562381594526414 1106 6 -0.2020791077312083 1106 7 -0.40198946433567784 1106 8 -0.08103721804022257 1106 9 -0.49351002015845324 1106 10-11 -0.6266538314025141 1106 12-13 -0.4200454264570368 1106 14-15 -0.40450641531968046 1106 16-17 -0.38339996057343484 1106 18-19 -0.25896068828372876 1106 20-21 -0.2818405127844983 1106 22-23 -0.12280394039398601 1106 24-25 -0.25857021050315865 1106 26-27 -0.5221133057739209 1106 28-29 -0.42413038481858933 1106 30-31 -0.3245036777297585 1106 32-33 -0.05272105445826725 1106 34-35 -0.43306057258126884 1106 36-37 -0.5705767009040343 1106 38-39 -0.27466575281189165 1106 40-41 -0.2995689674917159 1106 42-43 -0.6876383135447668 1106 44-45 -0.26597297962548083 1106 46-47 -0.11564878200806561 1106 48-49 -0.001429624881836844 1106 50-51 -0.16016845660472967 1106 52-53 0.23877898600323988 1106 54-55 0.016806496160775453 1106 56-57 -0.10430081230620658 1106 58-59 -0.18730461341858273 1106 60-61 0.011211608193328004 1106 62-63 -0.016415057874759498 1106 64-65 0.23829170366792596 1106 66-67 -0.33789777973979795 1106 68-69 -0.07231610807324529 1106 70-71 0.08002546130989074 1106 72-73 0.4196096283091286 1106 74-75 0.025499430869697193 1106 76-77 -0.22658483582494782 1106 78-79 0.020072320773909524 1106 80-81 -0.08170565553242781 1106 82-83 -0.14658872027148107 1106 84-85 -0.48291672415713194 1106 86-87 -0.374186354702541 1106 88-89 -0.2785605113356091 1106 90-91 -0.7935279620211695 1106 92-93 -0.8256272935085072 1106 94-95 -1.2166608381026869 1106 96-97 -0.5784674262606089 1106 98-99 -0.670780520183694 1106 100-101 0.2900204304629561 1107 1 -0.20841544422708935 1107 2 -0.009888786217274514 1107 3 -0.22301515295786345 1107 4 -0.04567577993341132 1107 5 -0.09154298684380535 1107 6 -0.21237259284483656 1107 7 -0.18727573163633338 1107 8 -0.342516486713734 1107 9 -0.21376526559355113 1107 10-11 -0.25285066263531775 1107 12-13 -0.44124222822937753 1107 14-15 -0.482343899631978 1107 16-17 -0.4097582351331468 1107 18-19 -0.3264339823757254 1107 20-21 -0.4533711015812756 1107 22-23 -0.6189446199879001 1107 24-25 -0.30492624606782925 1107 26-27 -0.8358230259584545 1107 28-29 -0.45110732906172046 1107 30-31 -0.5823121729483205 1107 32-33 -0.5341963165090675 1107 34-35 -0.35473109856833673 1107 36-37 -0.9709325832743119 1107 38-39 -0.7306017411219798 1107 40-41 -0.6047174776761963 1107 42-43 -0.4617060372527746 1107 44-45 -0.2430468487856885 1107 46-47 -0.043859530362702515 1107 48-49 -0.29543029658034925 1107 50-51 -0.2226806090449429 1107 52-53 -0.33116604077631706 1107 54-55 -0.25853391946740345 1107 56-57 -0.2636524464779839 1107 58-59 -0.3394354885710342 1107 60-61 -0.011263777591416613 1107 62-63 0.09772655266973374 1107 64-65 0.2070989674392223 1107 66-67 0.5187240481050566 1107 68-69 0.45749787857527124 1107 70-71 0.2835648411082623 1107 72-73 0.4534508599920848 1107 74-75 0.25893528660770926 1107 76-77 0.3519331851391172 1107 78-79 0.24677940719322677 1107 80-81 0.17198426722223914 1107 82-83 0.3834138133281755 1107 84-85 0.4764366776423117 1107 86-87 0.20788459787200608 1107 88-89 0.12468544227006007 1107 90-91 0.1765284253014059 1107 92-93 0.15134641008390304 1107 94-95 0.14001401857217033 1107 96-97 0.06932097692347838 1107 98-99 0.24100012900702694 1107 100-101 0.23127332495326058 1108 1 0.16292547778305178 1108 2 0.08169680250024669 1108 3 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2316 98-99 0.4758695067335488 2316 100-101 -0.44448257937407476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 5.0 5 13.0 6 37.0 7 75.0 8 196.0 9 389.0 10 662.0 11 1083.0 12 1552.0 13 2195.0 14 2922.0 15 4022.0 16 5788.0 17 8082.0 18 7065.0 19 3958.0 20 3322.0 21 3342.0 22 3270.0 23 3585.0 24 3966.0 25 4561.0 26 5457.0 27 6447.0 28 7744.0 29 9486.0 30 11887.0 31 14429.0 32 18009.0 33 23073.0 34 31857.0 35 44860.0 36 66184.0 37 95536.0 38 76417.0 39 7077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58725625678153 19.874310348071692 16.091285977408475 20.4471474177383 2 15.881142188463185 21.80250963839057 39.26069103864759 23.05565713449865 3 19.237914137350984 26.195317152678378 30.904911661146578 23.661857048824064 4 12.848888842452801 18.046619510819028 36.951656549403936 32.152835097324235 5 13.06406004100545 38.52359485279211 33.13206805817674 15.280277048025697 6 30.12422814514528 37.58042440262875 16.683139868980575 15.612207583245393 7 26.924595918964382 35.1219817993752 20.194752954205892 17.758669327454523 8 27.019882772095162 34.03182497309609 19.241675460500883 19.706616794307863 9 26.978299255049055 16.82001023915746 20.302368588772453 35.899321917021034 10-11 25.184774999738796 27.501436616480863 25.988548860632527 21.32523952314781 12-13 26.699474962777213 25.334273698508476 27.36650732701199 20.599744011702324 14-15 23.072792051070408 25.90809833770413 27.42997147663278 23.58913813459268 16-17 20.916613555390708 29.605792437650848 27.6489640689158 21.82862993804265 18-19 20.860402670539436 26.69536416921775 32.0957883628841 20.348444797358713 20-21 22.000083584958887 25.359363082613285 32.43660603274441 20.20394729968342 22-23 21.39309252456358 25.579244975488656 32.608232299803156 20.419430200144603 24-25 21.516440116601018 25.806438131458243 32.5019067818746 20.175214970066136 26-27 21.0160024406961 26.14553904277905 32.68923753322523 20.14922098329962 28-29 20.57616737524332 26.552347079615874 32.64419073371902 20.22729481142179 30-31 20.815893679932294 25.9353679305409 32.56574479422428 20.682993595302527 32-33 20.713397624097542 26.296037028136787 32.3786189675168 20.611946380248874 34-35 21.325030560750594 26.088850811296503 32.04009988402587 20.54601874392703 36-37 21.403913865699867 25.844051362957238 32.28030215962637 20.471732611716522 38-39 21.083887954362613 26.405219880682473 32.02505459142627 20.48583757352864 40-41 20.838984024824732 26.490476538746854 32.01209892279884 20.65844051362957 42-43 21.10569462513911 26.50438108375002 31.29363449691992 21.09628979419095 44-45 21.189622927354222 26.67739340305712 31.0510808579996 21.081902811589053 46-47 21.693744710639322 27.18391825391021 30.274576589942644 20.84776044550783 48-49 21.87930331936768 27.286832234539393 29.728871289611437 21.10499315648149 50-51 21.6633406818443 27.63987420463688 29.569328499336546 21.12745661418228 52-53 21.9311259938774 28.049962909174493 28.927396015087087 21.09151508186102 54-55 21.509753319890084 27.76577404895989 29.115357691383437 21.609114939766588 56-57 21.85830259844741 27.854583067776957 28.927187052689867 21.35992728108577 58-59 21.955783556748962 27.364461765105368 29.121104157306892 21.558650520838775 60-61 21.577561617786877 27.502585909665555 29.80712770736906 21.112724765178505 62-63 21.212504309849443 27.764102349782156 29.726050297249014 21.29734304311939 64-65 21.291074171202894 27.62691853600944 29.558566935879888 21.523440356907773 66-67 21.371211250535467 27.650322324497704 29.252959429950582 21.725506995016246 68-69 21.349479161224938 27.52139252541505 29.358589921743583 21.77053839161643 70-71 21.533157108378347 27.820417715832036 28.91694789522625 21.729477280563362 72-73 21.48154339626584 27.538840885582637 29.111387405836318 21.8682283123152 74-75 21.315000365684195 28.135741973231916 28.502575461545693 22.046682199538193 76-77 21.37089780693964 27.71008557010166 28.84297520661157 22.076041416347127 78-79 21.458557532572016 27.74466884684101 28.677581469214612 22.11919215137236 80-81 21.276864728192162 28.155697882166102 28.564846255916247 22.002591133725485 82-83 21.38034579169749 27.930181337947236 28.62947546149442 22.05999740886086 84-85 21.03891137584657 27.888889121072243 28.460303960513972 22.61189554256722 86-87 21.125053546614286 27.630575377960735 28.310539018503622 22.933832056921357 88-89 20.94116663706366 27.471032587685844 28.31670340922151 23.271097366028982 90-91 21.116624817530454 27.402981581155455 28.53808062411116 22.94231297720293 92-93 21.126775279149683 27.281807705126827 28.547502201957364 23.043914813766126 94-95 20.99382516116225 27.47082362528863 28.25840290039807 23.27694831315105 96-97 20.954435749286915 27.64635203895059 28.11714431987964 23.282067891882853 98-99 21.040759202974794 27.760787956230693 27.512958294196377 23.68549454659814 100-101 20.745813082764435 27.382705186897383 27.743322928955898 24.128158801382284 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 662.0 1 456.0 2 287.5 3 342.5 4 306.5 5 283.5 6 490.0 7 1036.0 8 1604.5 9 1832.5 10 1652.5 11 1424.5 12 1412.5 13 1468.5 14 1534.0 15 1552.0 16 1606.5 17 1689.0 18 1750.0 19 1797.0 20 1870.0 21 1946.5 22 2120.0 23 2422.0 24 2752.0 25 3171.5 26 3778.0 27 4481.0 28 5065.5 29 5811.0 30 6545.5 31 7129.5 32 7798.5 33 8369.0 34 9006.0 35 9599.0 36 10474.0 37 11417.5 38 11993.5 39 12142.0 40 12396.5 41 13324.5 42 14330.5 43 15186.0 44 15706.5 45 16224.5 46 16159.0 47 16932.0 48 19326.0 49 19152.5 50 17103.0 51 15974.0 52 15442.0 53 15103.0 54 14647.5 55 13599.5 56 12106.0 57 10898.5 58 9912.0 59 8793.0 60 7574.0 61 6430.0 62 5404.0 63 4151.0 64 2888.0 65 2175.5 66 1733.0 67 1463.5 68 1043.0 69 627.0 70 444.5 71 340.0 72 298.5 73 256.0 74 212.5 75 168.0 76 102.0 77 56.5 78 33.0 79 30.0 80 23.5 81 14.0 82 9.5 83 5.5 84 3.0 85 2.0 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04889720094868929 2 0.0 3 0.0 4 0.0 5 0.01713491657176291 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003656841951290865 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.0896239721662085E-4 24-25 0.0 26-27 6.268871916498626E-4 28-29 0.0028209923624243818 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.016194585784288118 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0896239721662085E-4 84-85 0.0012537743832997251 86-87 0.0 88-89 0.0 90-91 0.010552601059439353 92-93 7.31368390258173E-4 94-95 0.0 96-97 0.0 98-99 1.0448119860831043E-4 100-101 0.014731849003771773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 478555.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.20239467586698 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41608841275058 49.8285957600244 2 10.988219528766187 13.450106967692344 3 3.2403980907366403 5.94960368568569 4 1.3736162279153694 3.3627441005620833 5 0.7297967981235687 2.233265583597133 6 0.47856192613789433 1.7573481528200703 7 0.3472765396701953 1.4877909089795267 8 0.2344820355553911 1.1480689667569373 9 0.17671694306565844 0.9733950085875428 >10 0.9231855330787628 10.258798256044184 >50 0.05916726804164284 2.5400578571795376 >100 0.02941263020629734 3.358695362089055 >500 0.0013680293119208064 0.6122512734706772 >1k 0.0017100366399010083 3.039278116510849 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4973 1.0391700013582557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4097 0.8561189413964956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3013 0.6296037028136787 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1356 0.2833530106257379 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1081 0.22588835139116717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.19893220215022306 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 818 0.1709312409231959 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 627 0.13101942305482128 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 528 0.11033214573037582 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0448119860831044E-4 0.0 16-17 0.0 0.0 0.0 3.1344359582493133E-4 0.0 18-19 0.0 0.0 0.0 6.268871916498626E-4 0.0 20-21 0.0 0.0 0.0 6.268871916498626E-4 0.0 22-23 0.0 0.0 0.0 9.40330787474794E-4 0.0 24-25 0.0 0.0 0.0 0.0012537743832997251 0.0 26-27 0.0 0.0 0.0 0.0019851427735578984 0.0 28-29 0.0 0.0 0.0 0.0041792479443324175 0.0 30-31 0.0 0.0 0.0 0.008045052292839904 0.0 32-33 0.0 0.0 0.0 0.01211981903856401 0.0 34-35 1.0448119860831044E-4 0.0 0.0 0.015254254996813325 0.0 36-37 4.1792479443324176E-4 0.0 0.0 0.018702134550887567 0.0 38-39 4.1792479443324176E-4 0.0 0.0 0.023403788488261537 0.0 40-41 4.1792479443324176E-4 0.0 0.0 0.02841888602146044 0.0 42-43 4.1792479443324176E-4 0.0 0.0 0.04294177262801559 0.0 44-45 4.1792479443324176E-4 0.0 0.0 0.07031584666339293 0.0 46-47 4.1792479443324176E-4 0.0 0.0 0.09549581552799574 0.0 48-49 4.1792479443324176E-4 0.0 0.0 0.15337839955699972 0.0 50-51 4.1792479443324176E-4 0.0 0.0 0.21251475796930341 0.0 52-53 4.1792479443324176E-4 0.0 0.0 0.27791998829810577 0.0 54-55 4.1792479443324176E-4 0.0 0.0 0.4019391710461703 0.0 56-57 4.1792479443324176E-4 0.0 0.0 0.5264807597872763 0.0 58-59 4.1792479443324176E-4 0.0 0.0 0.6811129337275756 0.0 60-61 4.1792479443324176E-4 0.0 0.0 0.8702239032086176 0.0 62-63 4.1792479443324176E-4 0.0 0.0 1.0949629614150933 0.0 64-65 4.1792479443324176E-4 0.0 0.0 1.293790682366708 0.0 66-67 4.1792479443324176E-4 0.0 0.0 1.5581281148457335 0.0 68-69 4.1792479443324176E-4 0.0 0.0 1.8972740855283092 0.0 70-71 4.1792479443324176E-4 0.0 0.0 2.185537712488638 0.0 72-73 4.1792479443324176E-4 0.0 0.0 2.566267200217321 0.0 74-75 4.1792479443324176E-4 0.0 0.0 3.014909467041406 0.0 76-77 4.1792479443324176E-4 0.0 0.0 3.411311134561336 0.0 78-79 4.1792479443324176E-4 0.0 0.0 3.8066679900951823 0.0 80-81 4.1792479443324176E-4 0.0 0.0 4.305879157045689 0.0 82-83 4.1792479443324176E-4 0.0 0.0 4.98176803084285 0.0 84-85 4.1792479443324176E-4 0.0 0.0 5.678239700765847 0.0 86-87 4.1792479443324176E-4 0.0 0.0 6.419429323693201 0.0 88-89 4.1792479443324176E-4 0.0 0.0 7.379089132910533 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3550 0.0 30.129559 1 GTATCAA 5140 0.0 28.763113 1 GTACACG 70 0.008354323 27.164429 1 GTAAGGT 185 8.923962E-9 25.67997 4 GGGCGTA 115 1.2796809E-4 24.784163 9 TATCAAC 6080 0.0 24.30094 2 ATCAACG 6115 0.0 24.084158 3 TCAACGC 6230 0.0 23.639585 4 CAACGCA 6255 0.0 23.46915 5 ATGGGTA 245 2.9467628E-10 23.269197 5 AACGCAG 6405 0.0 22.914734 6 GAGTACT 3695 0.0 22.819427 12-13 TAAGGTG 230 3.607056E-9 22.72119 5 TATACTC 105 0.0021922307 22.62283 5 GGTGTGC 445 0.0 22.417133 8 GTACTTT 3780 0.0 22.303963 14-15 GGTAAGG 215 4.4001354E-8 22.096716 3 TGCACCG 130 2.9347165E-4 21.926743 5 GCACCGT 130 2.9388675E-4 21.922161 6 CGTCTAT 175 4.042544E-6 21.713377 6 >>END_MODULE