FastQCFastQC Report
Thu 26 May 2016
SRR937512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732390
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57480.7848277557039282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.5741476535725502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29770.4064774232307923No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14580.19907426371195674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11330.1546989991671104No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10760.146916260462322No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10520.14363931784977949No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.1339450292876746No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8620.11769685550048473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.10090252461120441No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG702.0514283E-433.9318734
GGTATCA40900.032.4053541
GTATCAA61550.030.5634921
GTACATG44750.026.1142751
TATCAAC73650.025.2179722
CGCGCGT950.001225431125.0024327
TACATGG46450.024.6454892
ATCAACG75350.024.585983
TCAACGC75850.024.4255814
CAACGCA76100.024.3486735
GAGTACT43450.024.32630212-13
AACGCAG76850.024.1695616
ACATGGG46200.023.8535023
GTTCTAA3500.023.0736751
GTACTTT45950.022.69030214-15
GTATAAG2755.0931703E-1122.456731
CATGGGA28450.021.7068564
GTGTAAG2858.367351E-1121.6687761
AGCGCGC1100.00287246521.5959685
GTACGTG1100.002874749221.5930081
Produced by FastQC (version 0.11.5)