##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937512_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732390 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.635603981485275 33.0 31.0 34.0 30.0 34.0 2 31.703846311391473 33.0 31.0 34.0 30.0 34.0 3 31.72785947377763 33.0 31.0 34.0 30.0 34.0 4 35.374924562050275 37.0 35.0 37.0 33.0 37.0 5 35.391178197408486 37.0 35.0 37.0 33.0 37.0 6 35.42652138887751 37.0 35.0 37.0 33.0 37.0 7 35.30101721760264 37.0 35.0 37.0 33.0 37.0 8 35.31287838446729 37.0 35.0 37.0 33.0 37.0 9 36.91182157047475 39.0 37.0 39.0 33.0 39.0 10-11 36.792559974876774 39.0 37.0 39.0 33.0 39.0 12-13 36.63352926719371 39.0 37.0 39.0 32.0 39.0 14-15 37.7307452313658 40.0 37.5 41.0 32.5 41.0 16-17 37.688087630907035 40.0 37.0 41.0 32.0 41.0 18-19 37.69927224566146 40.0 37.5 41.0 32.0 41.0 20-21 37.607485765780524 40.0 37.0 41.0 32.0 41.0 22-23 37.442437772225176 40.0 37.0 41.0 32.0 41.0 24-25 37.35627739319215 40.0 37.0 41.0 32.0 41.0 26-27 37.3327428009667 40.0 37.0 41.0 32.0 41.0 28-29 37.235843607913814 39.5 37.0 41.0 31.5 41.0 30-31 36.97551577711329 39.0 36.0 41.0 31.0 41.0 32-33 36.686881989104165 39.0 35.5 41.0 30.5 41.0 34-35 36.678265678122315 39.0 35.0 41.0 30.0 41.0 36-37 36.51259643086334 39.0 35.0 41.0 30.0 41.0 38-39 36.35824355875968 39.0 35.0 41.0 30.0 41.0 40-41 36.10793771078251 39.0 35.0 41.0 29.5 41.0 42-43 35.77569259547509 39.0 35.0 41.0 28.5 41.0 44-45 35.45201805049223 39.0 35.0 40.0 27.0 41.0 46-47 34.98916082961264 38.5 35.0 40.0 25.0 41.0 48-49 34.56170414669779 38.0 34.0 40.0 23.0 41.0 50-51 33.517937847321775 37.5 33.0 39.5 21.0 40.5 52-53 33.60973183686288 38.0 33.0 39.5 21.0 40.5 54-55 33.94746924452819 38.0 34.0 40.0 20.5 41.0 56-57 33.7002287032865 38.0 33.5 40.0 19.0 41.0 58-59 33.408782206201614 38.0 33.0 40.0 18.0 41.0 60-61 33.07951364710059 37.0 33.0 40.0 14.0 41.0 62-63 32.75370772402682 36.5 32.0 40.0 12.0 41.0 64-65 32.30749327544068 36.0 32.0 39.0 8.0 41.0 66-67 31.93969538087631 35.5 31.0 39.0 7.0 40.5 68-69 31.453608050355683 35.0 31.0 39.0 2.0 40.0 70-71 30.961411952648177 35.0 30.5 38.0 2.0 40.0 72-73 30.50460205628149 35.0 30.0 37.0 2.0 39.5 74-75 30.048914512759595 34.5 29.5 37.0 2.0 39.0 76-77 29.581545351520365 34.0 29.0 36.0 2.0 39.0 78-79 29.073925094553445 34.0 29.0 36.0 2.0 38.0 80-81 28.582185720722563 34.0 28.0 35.0 2.0 37.0 82-83 28.10055230136949 34.0 27.0 35.0 2.0 37.0 84-85 27.58082578953836 33.5 26.0 35.0 2.0 36.0 86-87 27.144248283018612 33.0 25.5 35.0 2.0 36.0 88-89 26.680078919701252 33.0 25.0 35.0 2.0 35.0 90-91 26.16140376029165 32.5 24.0 35.0 2.0 35.0 92-93 25.645147394147926 32.0 21.5 35.0 2.0 35.0 94-95 25.082142710850775 32.0 19.0 34.0 2.0 35.0 96-97 24.340016930870163 31.5 8.5 34.0 2.0 35.0 98-99 23.428233591392562 31.0 2.0 34.0 2.0 35.0 100-101 21.003560261609252 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1499256054163851 1101 2 -0.24315347875342752 1101 3 -0.1392387690828336 1101 4 -0.22057015443487415 1101 5 -0.0996739616955864 1101 6 -0.3563890631753992 1101 7 -0.25824405732872435 1101 8 -0.30009391804478724 1101 9 -0.07791097796166468 1101 10-11 -0.2708728210744624 1101 12-13 -0.07291579652599722 1101 14-15 -0.47568226465863006 1101 16-17 -0.4373257814476901 1101 18-19 -0.16100488139591818 1101 20-21 -0.03841391454753307 1101 22-23 -0.3620906332861722 1101 24-25 -0.3576704418908818 1101 26-27 -0.3534552747699422 1101 28-29 -0.5008243600879538 1101 30-31 -0.36832310841563753 1101 32-33 -0.46658182743625076 1101 34-35 -0.3801339467725384 1101 36-37 -0.5131473148078953 1101 38-39 -0.48392524032774986 1101 40-41 -0.6848668193471994 1101 42-43 -0.5199560789487876 1101 44-45 -0.3105213220354983 1101 46-47 -0.34607179609364636 1101 48-49 -0.38175106422148275 1101 50-51 -0.24956114746219527 1101 52-53 -0.3861287571580121 1101 54-55 -0.4734042749773266 1101 56-57 -0.21260706341309543 1101 58-59 -0.28819995465462256 1101 60-61 -0.3653843377764474 1101 62-63 -0.22089927651239805 1101 64-65 -0.14317921938777545 1101 66-67 -0.36760151828959664 1101 68-69 -0.3570811695924512 1101 70-71 -0.27370069194617486 1101 72-73 -0.27674956561780206 1101 74-75 -0.4139198803546833 1101 76-77 -0.23658990557866488 1101 78-79 -0.2292902939375736 1101 80-81 -0.0021173113567236612 1101 82-83 -0.03639339621605231 1101 84-85 -0.11209892044912095 1101 86-87 -0.0846617872956621 1101 88-89 -0.19106850329318448 1101 90-91 -0.49175029715909346 1101 92-93 -0.37586729148981846 1101 94-95 -0.5052717366069785 1101 96-97 -0.360149090610026 1101 98-99 -0.3758556957928221 1101 100-101 -0.019694417299643163 1102 1 -0.11561880958581483 1102 2 -0.09802973173797724 1102 3 -0.07938160669182892 1102 4 -0.1774722191640805 1102 5 -0.052077903929451 1102 6 -0.12702110381785303 1102 7 -0.043306062314364624 1102 8 -0.21865965174415436 1102 9 -0.3700470207919153 1102 10-11 -0.04487017047966191 1102 12-13 -0.16915184671899652 1102 14-15 0.03150431828289868 1102 16-17 -0.10820500057403848 1102 18-19 -0.017129385724174995 1102 20-21 -0.11001994359889267 1102 22-23 -0.16604838154277957 1102 24-25 -0.1312084074541886 1102 26-27 -0.3634765216013278 1102 28-29 -0.22795745781285603 1102 30-31 -0.38488117432288504 1102 32-33 -0.4361155015603444 1102 34-35 -0.25271107171863605 1102 36-37 -0.1942252759535421 1102 38-39 -0.13709701359670845 1102 40-41 -0.08077654135029633 1102 42-43 -0.026587299169037237 1102 44-45 0.07495194587442455 1102 46-47 0.14988446909205067 1102 48-49 0.10168245528458186 1102 50-51 -0.09619504983195526 1102 52-53 0.07479442818378601 1102 54-55 0.19938768488508885 1102 56-57 0.1664243124770124 1102 58-59 -0.16273951898939742 1102 60-61 0.07584661107338064 1102 62-63 0.1464281981649549 1102 64-65 0.2523560535077962 1102 66-67 -0.07591171150954423 1102 68-69 -0.09704230472019404 1102 70-71 -0.2837584896384726 1102 72-73 -0.21024777225901303 1102 74-75 -0.03837240630842231 1102 76-77 -0.12813666438505322 1102 78-79 -0.2691935787818487 1102 80-81 -0.2825144798482988 1102 82-83 -0.30582741007908254 1102 84-85 -0.11257960371355935 1102 86-87 -0.10252253640424414 1102 88-89 -0.37002888110813004 1102 90-91 -0.4319425704648694 1102 92-93 -0.3435358198837868 1102 94-95 -0.46599507551497865 1102 96-97 -0.2305297454752342 1102 98-99 -0.3144896928266796 1102 100-101 -0.07498587754598063 1103 1 0.05805749488218481 1103 2 -0.059013460437476795 1103 3 -0.19597737182750308 1103 4 -0.06291337089335514 1103 5 -0.048951026240601436 1103 6 -0.15272110622129276 1103 7 0.03802159889649204 1103 8 -0.07748869493399724 1103 9 0.05738706481868405 1103 10-11 -0.02501774180178984 1103 12-13 0.00792292435317421 1103 14-15 -0.08310380054326316 1103 16-17 0.08538021345244573 1103 18-19 -0.18408025937524286 1103 20-21 -0.1781947699502311 1103 22-23 -0.2942101091366922 1103 24-25 -0.30324856896547914 1103 26-27 -0.43601239296913263 1103 28-29 -0.4074819368668585 1103 30-31 -0.4971092849773413 1103 32-33 -0.19200934153919036 1103 34-35 -0.19298013702014316 1103 36-37 -0.16888398722808518 1103 38-39 -0.3560565636724178 1103 40-41 -0.18141044910620252 1103 42-43 -0.09463954999221613 1103 44-45 -0.3203457203836635 1103 46-47 -0.3203537119832518 1103 48-49 0.1022046030001107 1103 50-51 -0.019507833738508396 1103 52-53 -0.11836177149415761 1103 54-55 0.09365066947903244 1103 56-57 0.20846411347203997 1103 58-59 0.37302129048978117 1103 60-61 0.23963876143269403 1103 62-63 0.23552798906553107 1103 64-65 0.08011880977475982 1103 66-67 0.43991074624996784 1103 68-69 0.257337366969665 1103 70-71 0.25645169134069334 1103 72-73 0.15068368158288692 1103 74-75 0.3160312997987198 1103 76-77 0.26352159023556254 1103 78-79 0.42282999898150564 1103 80-81 0.24176148333097203 1103 82-83 0.13940656497318926 1103 84-85 0.043254751082507426 1103 86-87 -0.005172931770406564 1103 88-89 0.23007927376965753 1103 90-91 0.35944109093825816 1103 92-93 0.2330462456340321 1103 94-95 -0.08068971824336302 1103 96-97 -0.27665659912966944 1103 98-99 0.21168979620160755 1103 100-101 0.837655625085791 1104 1 -0.14704754377745033 1104 2 -0.076247421913056 1104 3 -0.03967935459354166 1104 4 -0.10964912091486667 1104 5 -0.0798664421543549 1104 6 -0.0625685573896888 1104 7 -0.02554246300809382 1104 8 -0.06552203195505513 1104 9 -0.10141245823573541 1104 10-11 -0.061191236903262336 1104 12-13 -0.03651414464413705 1104 14-15 0.05462345542162694 1104 16-17 0.017261812941079313 1104 18-19 -0.024739870197478808 1104 20-21 -0.11262397180392014 1104 22-23 -0.21185402254894115 1104 24-25 -0.058568110212661395 1104 26-27 0.13509613673548415 1104 28-29 0.01866217937018888 1104 30-31 -0.19100671709249184 1104 32-33 -0.126613710262383 1104 34-35 -0.14885345142316453 1104 36-37 -0.1166535290898949 1104 38-39 0.02264498885920574 1104 40-41 0.23548977338645471 1104 42-43 0.2954785800860904 1104 44-45 0.23461672629800034 1104 46-47 0.32755780723593375 1104 48-49 0.29324404934987314 1104 50-51 0.1089816746879464 1104 52-53 9.606707622324961E-4 1104 54-55 0.14437838026316996 1104 56-57 0.26189922797585297 1104 58-59 0.23551222483600753 1104 60-61 0.3879989536860151 1104 62-63 0.5934307806722643 1104 64-65 0.6132775908591981 1104 66-67 0.6910508674531464 1104 68-69 0.6721755373670781 1104 70-71 0.7305177753470318 1104 72-73 0.8677461639125461 1104 74-75 0.6911741990869906 1104 76-77 0.578825028423168 1104 78-79 0.5922716776332742 1104 80-81 0.5259774118383014 1104 82-83 0.4090068157383975 1104 84-85 0.779312082891586 1104 86-87 0.8660579074325518 1104 88-89 0.680055303566462 1104 90-91 0.8598098632950837 1104 92-93 0.8254114286065821 1104 94-95 0.793918461127511 1104 96-97 1.1457782818183198 1104 98-99 1.2351191332718336 1104 100-101 1.5113474051499587 1105 1 -0.09813659725795887 1105 2 -0.12892850841948444 1105 3 -0.31087725037627933 1105 4 -0.24300111581067796 1105 5 -0.21267876483798887 1105 6 -0.2864770451222327 1105 7 -0.03167640090880752 1105 8 -0.08742583075987653 1105 9 -0.20766147577172234 1105 10-11 -0.21077626932142124 1105 12-13 -0.18029854139231105 1105 14-15 -0.19733537818027713 1105 16-17 -0.19840070194823767 1105 18-19 -0.27116286056996586 1105 20-21 -0.25392184144366325 1105 22-23 -0.1894656115473623 1105 24-25 -0.2688528167259747 1105 26-27 -0.22927759551816962 1105 28-29 -0.38342430100028224 1105 30-31 -0.2759958437902199 1105 32-33 -0.33341702664059625 1105 34-35 -0.2672181941836129 1105 36-37 -0.1070268528066407 1105 38-39 0.06090455438103959 1105 40-41 -0.008759397140714498 1105 42-43 -0.031010919422577388 1105 44-45 0.11142813775939686 1105 46-47 -0.14266985542017352 1105 48-49 -0.02586698778747376 1105 50-51 -0.3808750936169929 1105 52-53 -0.29988347072602295 1105 54-55 -0.15634135080858869 1105 56-57 -0.14006578411633797 1105 58-59 0.12866175018505288 1105 60-61 0.10260355972950919 1105 62-63 0.15054524918340917 1105 64-65 0.3951163600030654 1105 66-67 0.28563178508084874 1105 68-69 0.16318394263569402 1105 70-71 0.3443789450907566 1105 72-73 0.383486603434374 1105 74-75 0.43860087890081445 1105 76-77 0.43050385452682605 1105 78-79 0.4508998342323629 1105 80-81 0.3942557818264767 1105 82-83 0.45077214911207975 1105 84-85 0.4001003069412903 1105 86-87 0.10892259177054342 1105 88-89 0.21412862669478017 1105 90-91 0.22383306079409948 1105 92-93 0.40638873967717615 1105 94-95 0.43220988580330655 1105 96-97 0.47388721826897395 1105 98-99 0.2786459668333947 1105 100-101 0.5877358211951993 1106 1 -0.1922272079716869 1106 2 -0.15220777892823634 1106 3 -0.3014894852447547 1106 4 0.11185710822494599 1106 5 -0.0053510893600332565 1106 6 -0.04503023173199949 1106 7 -0.3727109857491371 1106 8 -0.44166974883837185 1106 9 -0.46666105804366964 1106 10-11 -0.9089250436932801 1106 12-13 -0.32838196531140085 1106 14-15 -0.5297170248244711 1106 16-17 -0.25868342310131 1106 18-19 -0.5679633211239192 1106 20-21 -0.222437783560828 1106 22-23 -0.48453372930220695 1106 24-25 -0.5838952389402081 1106 26-27 -0.7279278775723554 1106 28-29 -0.22380011583326365 1106 30-31 -0.5860226041278196 1106 32-33 -0.3778646953787401 1106 34-35 -0.254185547039782 1106 36-37 -0.629614005270362 1106 38-39 -0.2713372645262453 1106 40-41 -0.5394150195613676 1106 42-43 -0.5797979284312262 1106 44-45 -0.6429073996647077 1106 46-47 -0.7299574664486599 1106 48-49 -0.23880239907408196 1106 50-51 -0.6240840875242455 1106 52-53 -0.502124107825658 1106 54-55 -0.5820366573940632 1106 56-57 -0.4233737456385498 1106 58-59 -0.08416016076281352 1106 60-61 -0.26422017424442146 1106 62-63 -0.29235530462804604 1106 64-65 -0.0889614146414246 1106 66-67 -0.09870902952395966 1106 68-69 -0.4009203466240159 1106 70-71 -0.026874227360721648 1106 72-73 -0.36714613170147103 1106 74-75 -0.20286032680041544 1106 76-77 -1.3749259348116993E-4 1106 78-79 0.044123395966604306 1106 80-81 -0.4705512734481907 1106 82-83 -0.28121552264494554 1106 84-85 -0.42292692260904374 1106 86-87 -0.35023659303960386 1106 88-89 -0.12019364172065039 1106 90-91 -0.05504253698410366 1106 92-93 -0.2561607119523721 1106 94-95 -0.1392924023401214 1106 96-97 0.22798229878985765 1106 98-99 -0.2685549626992767 1106 100-101 0.2534767385353618 1107 1 0.07276925011568025 1107 2 -0.1540794829817571 1107 3 -0.04949105942814569 1107 4 -0.09196973160189259 1107 5 -0.10931173477522549 1107 6 0.042551035485629995 1107 7 -0.06505730609546134 1107 8 4.0502487276228294E-4 1107 9 -0.11879328944863232 1107 10-11 -0.049516936648807075 1107 12-13 -0.10040557806228634 1107 14-15 0.0028575521137454984 1107 16-17 -0.1224040055491642 1107 18-19 -0.13265674890825352 1107 20-21 -0.019940341608844392 1107 22-23 -0.07112798353282557 1107 24-25 -0.28642731292682555 1107 26-27 0.02136924954294983 1107 28-29 0.12328134815729896 1107 30-31 -0.07613988079054934 1107 32-33 -0.006508536386213848 1107 34-35 0.23195061982365672 1107 36-37 0.026326962852429858 1107 38-39 -0.014201491208652328 1107 40-41 -0.029368581151295814 1107 42-43 0.07172115854240957 1107 44-45 0.1584046821928311 1107 46-47 0.3248894442164314 1107 48-49 0.5007521070258747 1107 50-51 0.565013905391929 1107 52-53 0.4081277615171146 1107 54-55 0.33523843115374774 1107 56-57 0.42446411347203394 1107 58-59 0.4705639635976695 1107 60-61 0.5331202429141797 1107 62-63 0.6308709842346047 1107 64-65 0.7132267002739638 1107 66-67 0.4956853034158293 1107 68-69 0.42844525746885864 1107 70-71 0.46432608747595694 1107 72-73 0.5128318297310379 1107 74-75 0.6223887877214302 1107 76-77 0.46735089780399974 1107 78-79 0.4124531873873032 1107 80-81 0.5584506942810528 1107 82-83 0.6490780625577308 1107 84-85 0.5552611923063395 1107 86-87 1.0264277123519747 1107 88-89 0.8483691288421191 1107 90-91 0.8694604146097547 1107 92-93 0.877767662703274 1107 94-95 1.025039750355667 1107 96-97 1.0903160256690434 1107 98-99 1.1981471230937188 1107 100-101 1.2743126299167074 1108 1 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-1.74908130947723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3070.0 3 779.0 4 573.0 5 686.0 6 825.0 7 909.0 8 1133.0 9 1520.0 10 1852.0 11 2486.0 12 3273.0 13 4449.0 14 4976.0 15 6348.0 16 8298.0 17 10850.0 18 8949.0 19 6172.0 20 5901.0 21 6013.0 22 6546.0 23 7158.0 24 7966.0 25 8936.0 26 10191.0 27 11877.0 28 13836.0 29 16428.0 30 19685.0 31 24608.0 32 30129.0 33 38996.0 34 52421.0 35 72696.0 36 99986.0 37 128144.0 38 93226.0 39 10496.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21247281332937 18.853896197219534 15.298173959104677 21.635457030346416 2 15.111932129173509 20.612205801860974 40.45607553366174 23.819786535303777 3 18.894352861333683 25.85667608038881 30.127605192871844 25.121365865405664 4 12.329724973761738 16.876504338378467 37.529949931787364 33.263820756072434 5 13.331408523168875 37.82265656643058 33.42136375080732 15.424571159593217 6 30.501688994979553 36.67528044981467 17.51613715624709 15.306893398958682 7 27.58979861950406 34.07303789862402 20.565502383634428 17.771661098237495 8 26.714731285660132 33.623062258188575 19.75644705022561 19.90575940592569 9 27.004990838042037 16.567748539492214 20.511701168269383 35.91555945419637 10-11 24.883254316716123 26.929246367607934 26.72011693729402 21.46738237838193 12-13 26.338956372968347 24.62583404619333 27.700188195038493 21.33502138579983 14-15 22.964407973019114 25.475973311966847 27.124919345402844 24.434699369611195 16-17 21.379787964972888 29.124910694000008 27.295882753882246 22.199418587144866 18-19 21.266327408286305 26.766719834024784 30.99342216456153 20.973530593127386 20-21 22.398833548747305 25.599937599510646 31.017080081312216 20.98414877042983 22-23 21.832914711253395 25.633013374239926 31.1732616464403 21.360810268066377 24-25 21.678080059551707 25.887682361706833 31.156202128234582 21.27803545050688 26-27 21.344230775809866 26.232547955703193 31.197909004758795 21.225312263728142 28-29 21.033449709106186 26.511026045637003 31.270982196012202 21.184542049244605 30-31 21.123917356039232 26.070702971835004 31.148668391552214 21.65671128057355 32-33 21.034760955561897 26.343369730412537 30.985827788920204 21.636041525105362 34-35 21.824388227581117 26.30672727247842 30.60566119599301 21.263223303947456 36-37 21.701627172174277 26.040023153438053 30.714910294111608 21.543439380276062 38-39 21.27041752220757 26.412831300447266 30.739467866670406 21.577283310674755 40-41 21.371363906305163 26.464631813442292 30.66976100098796 21.494243279264584 42-43 21.412233039867917 26.583970862832118 30.05191238322793 21.95188371407204 44-45 21.42162555370256 26.599758160376485 29.948901627935143 22.029714657985807 46-47 21.767363941143987 27.060463531155627 29.44018898745672 21.731983540243665 48-49 21.820224965165714 27.06268255493415 29.069315441238867 22.047777038661266 50-51 21.671815395817966 27.42844589591309 28.881769940090418 22.017968768178527 52-53 22.07656025730445 27.692740485409583 28.3081438446317 21.922555412654273 54-55 21.603425078461726 27.32418482612498 28.592369947892927 22.480020147520364 56-57 21.779484049657345 27.64064655266788 28.446990015983186 22.132879381691588 58-59 22.078753645337013 27.192042134383726 28.451380798591323 22.277823421687934 60-61 21.874923042825127 27.212946317411063 29.174430790531463 21.737699849232346 62-63 21.556593737750067 27.410358729270467 29.06208546919671 21.97096206378276 64-65 21.464079346196968 27.414086426281276 28.922999465744365 22.198834761777395 66-67 21.512214943604395 27.378479918129234 28.679464446961862 22.429840691304513 68-69 21.37660512833844 27.406982765756442 28.830502688810157 22.38590941709496 70-71 21.678653202707114 27.477272556248245 28.503145824368797 22.340928416675844 72-73 21.620924565553796 27.383827285999075 28.638422817644106 22.356825330803026 74-75 21.24966391619558 28.00438396950377 28.230355431285314 22.515596683015335 76-77 21.527799159904784 27.493515228521908 28.36946399626138 22.609221615311927 78-79 21.567378472851768 27.538285439046735 28.32701713958868 22.567318948512817 80-81 21.567535467203783 27.81459355022331 28.23337201155968 22.384498971013223 82-83 21.707024235064733 27.680582872975425 28.14079255673947 22.471600335220373 84-85 21.472916847672533 27.497863845734432 28.083057235251985 22.946162071341053 86-87 21.6506786661622 27.448777620949947 28.092174625774057 22.808369087113793 88-89 21.462505541659596 27.25545545600333 27.992427766861695 23.28961123547538 90-91 21.416089298934395 27.325698349250573 28.224147769647722 23.034064582167307 92-93 21.735311140696428 27.187918609058308 28.270777718333484 22.805992531911777 94-95 21.594305718705296 27.14874763713187 28.061671627863067 23.19527501629977 96-97 21.470561672290778 27.129841731186595 28.191991592567962 23.20760500395467 98-99 21.692314745062138 27.199758058943026 27.72154609640831 23.386381099586526 100-101 21.91942560611388 26.607034628629123 28.171417939137562 23.302121826119436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1492.0 1 920.5 2 396.0 3 457.5 4 406.5 5 381.0 6 589.5 7 1183.5 8 1900.0 9 2186.5 10 1925.0 11 1625.0 12 1652.0 13 1762.5 14 1836.0 15 1907.0 16 1948.5 17 1975.0 18 2045.5 19 2176.5 20 2294.0 21 2483.0 22 2766.0 23 3193.0 24 3739.5 25 4370.5 26 5346.0 27 6373.0 28 7175.5 29 8237.5 30 9504.0 31 10573.0 32 11666.5 33 12636.5 34 13614.0 35 14466.0 36 15783.0 37 17456.0 38 18403.5 39 18661.5 40 19131.0 41 20550.0 42 22115.0 43 23295.5 44 24029.5 45 24873.0 46 25121.0 47 26289.5 48 29478.0 49 29396.0 50 26649.5 51 25041.5 52 24110.0 53 23881.5 54 23629.5 55 21959.5 56 19539.5 57 17435.5 58 15729.0 59 13957.0 60 12050.5 61 10345.0 62 8746.0 63 6889.0 64 5080.5 65 3956.5 66 3017.5 67 2362.0 68 1804.5 69 1215.0 70 893.0 71 683.5 72 567.5 73 483.5 74 387.5 75 301.0 76 213.0 77 138.0 78 84.5 79 58.0 80 39.5 81 31.0 82 23.5 83 15.5 84 10.0 85 7.5 86 5.0 87 2.5 88 1.0 89 1.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24481492101202912 2 0.21709744808094047 3 0.21054356285585546 4 0.21668783025437266 5 0.21600513387675965 6 0.24276683187919004 7 0.24344952825680308 8 0.23307254331708516 9 0.22542634388781932 10-11 0.2378514179603763 12-13 0.24099182129739619 14-15 0.22535807425005805 16-17 0.23976296781769274 18-19 0.22918117396469095 20-21 0.22208113163751553 22-23 0.2211253567088573 24-25 0.2179849533718374 26-27 0.2235830636682642 28-29 0.2336187004191756 30-31 0.23396004860798209 32-33 0.2320484987506656 34-35 0.2412648998484414 36-37 0.2202378514179604 38-39 0.22044266033124427 40-41 0.2173705266319857 42-43 0.22385614221930938 44-45 0.2376466090470924 46-47 0.24024085528202188 48-49 0.24413222463441608 50-51 0.24426876390993868 52-53 0.25853711820205083 54-55 0.24290337115471264 56-57 0.22146670489766382 58-59 0.20351179016644136 60-61 0.19989349936509238 62-63 0.2073348898810743 64-65 0.2002348475538989 66-67 0.20235120632449924 68-69 0.20351179016644136 70-71 0.2048771829216674 72-73 0.2071300809677904 74-75 0.2110214503201846 76-77 0.22385614221930938 78-79 0.2179849533718374 80-81 0.2149128196725788 82-83 0.20856374336077774 84-85 0.20760796843211948 86-87 0.21593686423899833 88-89 0.21327434836630757 90-91 0.22269555837736726 92-93 0.2112262592334685 94-95 0.21184068597332023 96-97 0.21955515504034737 98-99 0.22303690656617375 100-101 0.19975696008956975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 732390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.198822774809344 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73114676687531 47.199900270845646 2 11.712413663519134 13.867222014638495 3 3.6840045742469627 6.5426620167729865 4 1.597999525928431 3.7839876291866608 5 0.8467637341388445 2.5063708114710614 6 0.5278473745248438 1.8748765905986786 7 0.3431613876650576 1.4220325120078974 8 0.25453310876603785 1.2054448316929556 9 0.17380769094271226 0.9260289623714812 >10 1.0162831102405498 10.878262950431987 >50 0.07253684439590395 2.956356939626023 >100 0.036499126102841284 3.8352519028792402 >500 0.0013860427630824736 0.5880400492118558 >1k 0.0013860427630824736 1.6275034013186738 >5k 2.3100712718041226E-4 0.7860591169464529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5748 0.7848277557039282 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.5741476535725502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2977 0.4064774232307923 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1458 0.19907426371195674 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1133 0.1546989991671104 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1076 0.146916260462322 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1052 0.14363931784977949 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 981 0.1339450292876746 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 862 0.11769685550048473 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 739 0.10090252461120441 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3653927552260408E-4 0.0 10-11 0.0 0.0 0.0 1.3653927552260408E-4 0.0 12-13 0.0 0.0 0.0 1.3653927552260408E-4 0.0 14-15 0.0 0.0 0.0 1.3653927552260408E-4 0.0 16-17 0.0 0.0 0.0 2.7307855104520817E-4 0.0 18-19 0.0 0.0 0.0 4.778874643291143E-4 0.0 20-21 0.0 0.0 0.0 6.826963776130204E-4 0.0 22-23 0.0 0.0 0.0 8.192356531356244E-4 0.0 24-25 0.0 0.0 0.0 9.557749286582285E-4 0.0 26-27 0.0 0.0 0.0 0.0010240445664195306 0.0 28-29 0.0 0.0 0.0 0.0023211676838842694 0.0 30-31 0.0 0.0 0.0 0.004505796092245934 0.0 32-33 0.0 0.0 0.0 0.0077827387047884325 0.0 34-35 0.0 0.0 0.0 0.010786602766285722 0.0 36-37 0.0 0.0 0.0 0.014951050669725147 0.0 38-39 0.0 0.0 0.0 0.019320307486448477 0.0 40-41 0.0 0.0 0.0 0.02539630524720436 0.0 42-43 0.0 0.0 0.0 0.03918677207498737 0.0 44-45 0.0 0.0 0.0 0.061442673985171836 0.0 46-47 0.0 0.0 0.0 0.08472262046177584 0.0 48-49 0.0 0.0 0.0 0.12506997637870532 0.0 50-51 0.0 0.0 0.0 0.17203948715848114 0.0 52-53 0.0 0.0 0.0 0.22863501686260052 0.0 54-55 0.0 0.0 0.0 0.31820478160542875 0.0 56-57 0.0 0.0 0.0 0.41002744439438005 0.0 58-59 0.0 0.0 0.0 0.5342099154821884 0.0 60-61 0.0 0.0 0.0 0.6650828110706044 0.0 62-63 0.0 0.0 0.0 0.8292030202487746 0.0 64-65 0.0 0.0 0.0 0.9777577520173678 0.0 66-67 0.0 0.0 0.0 1.1657040647742323 0.0 68-69 0.0 0.0 0.0 1.4179603763022435 0.0 70-71 0.0 0.0 0.0 1.6287087480713827 0.0 72-73 0.0 0.0 0.0 1.9098431163724245 0.0 74-75 0.0 0.0 0.0 2.2421114433566816 0.0 76-77 0.0 0.0 0.0 2.528639113040866 0.0 78-79 0.0 0.0 0.0 2.817692759322219 0.0 80-81 0.0 0.0 0.0 3.1866901514220567 0.0 82-83 0.0 0.0 0.0 3.670243995685359 0.0 84-85 0.0 0.0 0.0 4.1857480304209504 0.0 86-87 0.0 0.0 0.0 4.729037807725392 0.0 88-89 0.0 0.0 0.0 5.372342604350141 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 70 2.0514283E-4 33.931873 4 GGTATCA 4090 0.0 32.405354 1 GTATCAA 6155 0.0 30.563492 1 GTACATG 4475 0.0 26.114275 1 TATCAAC 7365 0.0 25.217972 2 CGCGCGT 95 0.0012254311 25.002432 7 TACATGG 4645 0.0 24.645489 2 ATCAACG 7535 0.0 24.58598 3 TCAACGC 7585 0.0 24.425581 4 CAACGCA 7610 0.0 24.348673 5 GAGTACT 4345 0.0 24.326302 12-13 AACGCAG 7685 0.0 24.169561 6 ACATGGG 4620 0.0 23.853502 3 GTTCTAA 350 0.0 23.073675 1 GTACTTT 4595 0.0 22.690302 14-15 GTATAAG 275 5.0931703E-11 22.45673 1 CATGGGA 2845 0.0 21.706856 4 GTGTAAG 285 8.367351E-11 21.668776 1 AGCGCGC 110 0.002872465 21.595968 5 GTACGTG 110 0.0028747492 21.593008 1 >>END_MODULE