FastQCFastQC Report
Thu 26 May 2016
SRR937512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732390
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.9660153743224239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56120.766258414232854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41440.5658187577656713No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18140.2476822457980038No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13660.18651265036387718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12080.16493944483130574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.14295662147216648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9140.12479689782766014No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7350.100356367509114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.033.3165741
GTATCAA69850.030.2036061
TAAGGTG3700.026.9576385
TGTACCG908.947963E-426.3871065
GTACATG44850.024.367311
TATCAAC87800.024.0729732
ATCAACG87500.024.0469463
TCAACGC88350.023.8155944
CAACGCA88850.023.7350315
AGTACTT53450.023.6421612-13
GAGTACT52850.023.55100812-13
TACATGG45750.023.3590772
AACGCAG90900.023.1475036
ACATGGG44800.022.9002383
CATGGGG17600.022.6689244
GTACTTT55500.022.29353714-15
ATGGGGG9250.022.079595
AGAGTAC80100.021.55882610-11
TACTTTT58800.021.40586714-15
ACGCAGA101550.020.6306027
Produced by FastQC (version 0.11.5)