##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732390 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04998020180505 31.0 31.0 34.0 30.0 34.0 2 32.25998579991535 33.0 31.0 34.0 30.0 34.0 3 32.29340515299226 34.0 31.0 34.0 30.0 34.0 4 35.9865973047147 37.0 35.0 37.0 35.0 37.0 5 35.89601851472576 37.0 35.0 37.0 35.0 37.0 6 35.942276655880065 37.0 35.0 37.0 35.0 37.0 7 35.87414492278704 37.0 35.0 37.0 35.0 37.0 8 35.84604513988449 37.0 35.0 37.0 35.0 37.0 9 37.516097980584114 39.0 37.0 39.0 35.0 39.0 10-11 37.42717609470364 39.0 37.0 39.0 34.0 39.0 12-13 37.31512855172791 39.0 37.0 39.0 34.0 39.0 14-15 38.41195742705389 40.0 38.0 41.0 34.0 41.0 16-17 38.394412812845616 40.0 38.0 41.0 33.5 41.0 18-19 38.44353145182211 40.0 38.0 41.0 34.0 41.0 20-21 38.366121192260955 40.0 38.0 41.0 34.0 41.0 22-23 38.20436447794208 40.0 37.5 41.0 33.5 41.0 24-25 38.119995494203906 40.0 37.0 41.0 33.0 41.0 26-27 38.017903029806526 40.0 37.0 41.0 33.0 41.0 28-29 37.85961782656781 40.0 37.0 41.0 33.0 41.0 30-31 37.86980843539644 40.0 37.0 41.0 33.0 41.0 32-33 37.731058589003126 40.0 36.5 41.0 33.0 41.0 34-35 37.55499119321673 40.0 36.0 41.0 33.0 41.0 36-37 37.326251723808355 40.0 36.0 41.0 32.5 41.0 38-39 37.15497890468193 40.0 35.5 41.0 32.0 41.0 40-41 36.919776348666694 39.0 35.0 41.0 31.0 41.0 42-43 36.63940864839771 39.0 35.0 41.0 31.0 41.0 44-45 36.23730048198364 39.0 35.0 41.0 30.0 41.0 46-47 35.71213765889758 39.0 35.0 41.0 28.5 41.0 48-49 35.239252993623616 39.0 35.0 40.0 26.5 41.0 50-51 34.92599639536313 38.5 35.0 40.0 25.5 41.0 52-53 34.65057892652821 38.0 34.0 40.0 24.0 41.0 54-55 34.39456437144145 38.0 34.0 40.0 23.5 41.0 56-57 34.09013845082538 38.0 34.0 40.0 23.0 41.0 58-59 33.78965646718278 37.5 33.0 40.0 22.0 41.0 60-61 33.42650841764633 37.0 33.0 40.0 21.5 41.0 62-63 33.03814497740275 36.5 33.0 39.5 19.5 41.0 64-65 32.632563251819384 36.0 32.0 39.0 18.0 40.5 66-67 32.200552984065865 35.5 31.5 39.0 15.5 40.0 68-69 31.74363317358238 35.0 31.0 38.0 11.5 40.0 70-71 31.317245593194883 35.0 31.0 37.5 8.0 40.0 72-73 30.84136252543044 35.0 30.5 37.0 7.0 39.0 74-75 30.361553953494724 34.0 30.0 36.5 3.5 39.0 76-77 29.135595106432365 33.5 28.5 35.0 2.0 37.5 78-79 29.740160979805843 34.0 29.5 35.5 2.0 37.5 80-81 29.53634265896585 34.0 29.5 35.0 2.0 37.0 82-83 29.17883572959762 34.0 29.0 35.0 2.0 37.0 84-85 28.81608842283483 34.0 29.0 35.0 2.0 36.0 86-87 28.402158685946013 34.0 29.0 35.0 2.0 36.0 88-89 27.916293914444488 33.5 27.5 35.0 2.0 35.5 90-91 27.395656685645626 33.0 26.0 35.0 2.0 35.0 92-93 27.009888174333348 33.0 25.5 35.0 2.0 35.0 94-95 26.524459645817117 33.0 25.0 35.0 2.0 35.0 96-97 25.997902756727974 32.5 24.5 34.5 2.0 35.0 98-99 25.213351493056976 32.0 21.0 34.0 2.0 35.0 100-101 22.83964622673712 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1617565877202125 1101 2 -0.0856899512656355 1101 3 -0.09705617668745958 1101 4 0.002533668346885065 1101 5 -0.1321892465491885 1101 6 -0.10604551025389952 1101 7 -0.09658824044737457 1101 8 -0.0866261681874505 1101 9 -0.1994457191248955 1101 10-11 -0.07984962779740812 1101 12-13 -0.17756624603313753 1101 14-15 -0.18329316250879657 1101 16-17 -0.15215839639299134 1101 18-19 -0.18727739352685546 1101 20-21 -0.16625094233966564 1101 22-23 -0.2056793633225027 1101 24-25 -0.20531903219480796 1101 26-27 -0.21949428215145161 1101 28-29 -0.3225052790176903 1101 30-31 -0.22080558255008498 1101 32-33 -0.21719819832731702 1101 34-35 -0.2037470848240588 1101 36-37 -0.08558682670086881 1101 38-39 -0.11954541750198189 1101 40-41 -0.0388247711963885 1101 42-43 -0.02175709301151585 1101 44-45 0.05278162685894472 1101 46-47 0.148434802893199 1101 48-49 0.28452446363282746 1101 50-51 0.3994374212825349 1101 52-53 0.2890496420811317 1101 54-55 0.3095669310988143 1101 56-57 0.45946299194489626 1101 58-59 0.31659947595780125 1101 60-61 0.3143486904318422 1101 62-63 0.3212038576347638 1101 64-65 0.42310369653812074 1101 66-67 0.47376114983728 1101 68-69 0.4205380445165119 1101 70-71 0.46834262020477624 1101 72-73 0.6112265362500828 1101 74-75 0.3561794659962949 1101 76-77 0.5045923987172394 1101 78-79 0.27899604497825337 1101 80-81 0.37137214659554374 1101 82-83 0.4388589251912123 1101 84-85 0.4980958232358361 1101 86-87 0.5589803549554127 1101 88-89 0.47075535113896905 1101 90-91 0.40471487544252227 1101 92-93 0.4995048148652792 1101 94-95 0.44327978210434793 1101 96-97 0.482731986516967 1101 98-99 0.42938818306447146 1101 100-101 0.9531777685427336 1102 1 -0.05634232709942566 1102 2 0.01275335019744972 1102 3 -0.0492744721025673 1102 4 -0.08548764159390032 1102 5 -0.13644755332398262 1102 6 -0.11949885662983917 1102 7 -0.21270375168610656 1102 8 -0.1954867279678325 1102 9 -0.20583186450792113 1102 10-11 -0.2212649086868268 1102 12-13 -0.19363363635426367 1102 14-15 -0.20424180197530717 1102 16-17 -0.20193830235998433 1102 18-19 -0.22679413329524323 1102 20-21 -0.34091015157888904 1102 22-23 -0.21999757143626653 1102 24-25 -0.24982982916134944 1102 26-27 -0.34152735147713287 1102 28-29 -0.3120640917195061 1102 30-31 -0.2337648349140622 1102 32-33 -0.15670810124402834 1102 34-35 -0.24636722934458533 1102 36-37 -0.14805961077205865 1102 38-39 -0.13826208785002336 1102 40-41 0.04790489968809197 1102 42-43 0.1635044954520879 1102 44-45 0.12000391920271625 1102 46-47 0.33050726300279365 1102 48-49 0.46835688713897383 1102 50-51 0.5813797182543325 1102 52-53 0.5153212964940579 1102 54-55 0.6199500051576976 1102 56-57 0.4854811569339006 1102 58-59 0.4492428131828845 1102 60-61 0.561476949769407 1102 62-63 0.5025500337310262 1102 64-65 0.4897966587881868 1102 66-67 0.4709015051431962 1102 68-69 0.6510919346851196 1102 70-71 0.6431459292409905 1102 72-73 0.7674959292428376 1102 74-75 0.5245916334886438 1102 76-77 0.5226811305338437 1102 78-79 0.5910517810968159 1102 80-81 0.43515865801308706 1102 82-83 0.376910738008732 1102 84-85 0.42296776374470824 1102 86-87 0.35872660257566125 1102 88-89 0.38610460907760924 1102 90-91 0.4875320264738363 1102 92-93 0.752067319467006 1102 94-95 0.6679587130311084 1102 96-97 0.9003613344916808 1102 98-99 0.7853141557380994 1102 100-101 1.412527952085906 1103 1 -0.01930408834152786 1103 2 0.024369184071559857 1103 3 0.06734652917126027 1103 4 0.045717444088801074 1103 5 -0.013760265127288562 1103 6 0.042756292744066116 1103 7 0.08645384198803185 1103 8 -0.03452102508129684 1103 9 -0.015062282466438148 1103 10-11 -0.06677227523572782 1103 12-13 0.0010313238687729154 1103 14-15 -0.10285020921447341 1103 16-17 -0.10086095960515706 1103 18-19 -0.1514336170359627 1103 20-21 -0.21176421459713168 1103 22-23 0.021812100013249847 1103 24-25 -7.497464829597789E-4 1103 26-27 0.03870193686377377 1103 28-29 -0.1275416555909743 1103 30-31 0.003386966465519947 1103 32-33 0.06560965778690075 1103 34-35 0.10190890207560699 1103 36-37 0.2052589413946393 1103 38-39 0.1479109118855888 1103 40-41 0.2749916879689067 1103 42-43 0.3176642522237856 1103 44-45 0.23812767241732047 1103 46-47 0.4819102721118398 1103 48-49 0.3873789266996255 1103 50-51 0.6127578517414847 1103 52-53 0.47718648744750425 1103 54-55 0.4391523028479867 1103 56-57 0.5338834690448451 1103 58-59 0.46159169678419687 1103 60-61 0.5673948241655395 1103 62-63 0.668720990541182 1103 64-65 0.6448595087893878 1103 66-67 0.7624059394931848 1103 68-69 0.6235412821506827 1103 70-71 0.8190616405395694 1103 72-73 1.023342301247549 1103 74-75 0.9040758548936978 1103 76-77 1.016840767133452 1103 78-79 0.9374315969931786 1103 80-81 0.9601874993138289 1103 82-83 1.1121872539795987 1103 84-85 0.9342963863322531 1103 86-87 1.0154135783249352 1103 88-89 1.0260870639529927 1103 90-91 1.0330814532529828 1103 92-93 1.1284492984609251 1103 94-95 1.0109875302974345 1103 96-97 1.1320657351934855 1103 98-99 1.3888351978704314 1103 100-101 1.6822914534198148 1104 1 0.014118979598634951 1104 2 -0.0024036890338692274 1104 3 -0.04482787556869283 1104 4 -0.04828877587161884 1104 5 -0.13543670426140864 1104 6 -0.0802415806686767 1104 7 -0.04950637976707384 1104 8 -0.0529842277462933 1104 9 -0.02871300587487724 1104 10-11 -0.04585980950082558 1104 12-13 0.010613258940253445 1104 14-15 -0.08362795011501589 1104 16-17 -0.2605609018308286 1104 18-19 -0.1775242612812704 1104 20-21 -0.12079729347753698 1104 22-23 -0.20009359260269832 1104 24-25 -0.17608563663795707 1104 26-27 -0.36030053391102257 1104 28-29 -0.16278092738849637 1104 30-31 -0.08990936670802085 1104 32-33 -0.1262810380865318 1104 34-35 -0.11751372333772281 1104 36-37 -0.07539526076636349 1104 38-39 -0.21724440273868595 1104 40-41 -0.10534475534463184 1104 42-43 -0.21407168218390638 1104 44-45 -0.046340250533837946 1104 46-47 -0.09921657328649758 1104 48-49 0.004239813474214316 1104 50-51 0.07700844485036384 1104 52-53 0.15061398065594034 1104 54-55 0.23592800320815854 1104 56-57 0.3620509162796637 1104 58-59 0.23312492931975015 1104 60-61 0.2305397148245376 1104 62-63 0.4641906221375933 1104 64-65 0.22992299909682146 1104 66-67 0.11288718741884196 1104 68-69 0.20152741631007132 1104 70-71 0.2846828375746391 1104 72-73 0.3787589525486972 1104 74-75 0.48605087671272784 1104 76-77 0.41978910212626275 1104 78-79 0.08285390280380156 1104 80-81 0.09312993394892999 1104 82-83 0.0022838723370881553 1104 84-85 0.2083557095120554 1104 86-87 0.12459478076866759 1104 88-89 0.33286571449200864 1104 90-91 0.27577054127894485 1104 92-93 0.5229220154527248 1104 94-95 0.7019440028669166 1104 96-97 0.6176682477624773 1104 98-99 0.796018268760772 1104 100-101 1.3345299016257393 1105 1 0.00993357781603521 1105 2 -0.08486509868794911 1105 3 -0.06374498376624871 1105 4 -0.13236499910761523 1105 5 -0.12731556214016138 1105 6 -0.023400310707323513 1105 7 -0.07924887542321812 1105 8 0.003612424436646222 1105 9 -0.12408031502047123 1105 10-11 0.05130472579684664 1105 12-13 -0.11005265622024751 1105 14-15 -0.15332468291254742 1105 16-17 -0.1729532721098508 1105 18-19 -0.187258535762588 1105 20-21 0.0472105679158048 1105 22-23 0.05377763084765519 1105 24-25 -0.17580907564448722 1105 26-27 0.00666059876176206 1105 28-29 0.04881597277466909 1105 30-31 -0.04014435949137152 1105 32-33 -0.020082877511349295 1105 34-35 -0.22460377210155968 1105 36-37 -0.14037910842748857 1105 38-39 -0.091430586289448 1105 40-41 -0.2457751145124618 1105 42-43 -0.080428835908954 1105 44-45 -0.12373193571496444 1105 46-47 -0.164425284369365 1105 48-49 -0.3023263665627809 1105 50-51 -0.28712907573409296 1105 52-53 -0.1317812729185448 1105 54-55 0.013992429310469845 1105 56-57 -0.1281362121177665 1105 58-59 0.0604020812627013 1105 60-61 -0.035079046266268676 1105 62-63 0.015820524685572934 1105 64-65 0.040560678136436934 1105 66-67 0.06094373699681199 1105 68-69 -0.03088889456802235 1105 70-71 0.12192164925341231 1105 72-73 0.2639457909353631 1105 74-75 0.30996432404971586 1105 76-77 0.3714859173036622 1105 78-79 -0.13855082529639517 1105 80-81 -0.1915387518651741 1105 82-83 -0.15827113261831371 1105 84-85 -0.049775309787531086 1105 86-87 -0.07610654092388458 1105 88-89 0.10153883536933961 1105 90-91 0.2309025129907063 1105 92-93 0.29207848761042854 1105 94-95 0.48503191984145744 1105 96-97 0.12962593813353251 1105 98-99 0.4580069356443168 1105 100-101 0.6794199539201458 1106 1 -0.2618728210288346 1106 2 -0.13378491479399912 1106 3 -0.16632939818055803 1106 4 -0.0672520779716308 1106 5 -0.2920969920806158 1106 6 -0.19615835723777764 1106 7 -0.2437377787174171 1106 8 -0.28810333795215115 1106 9 -0.3656593504548056 1106 10-11 -0.46738246141903517 1106 12-13 -0.4459645144314095 1106 14-15 -0.3054250827379619 1106 16-17 -0.33611819980311 1106 18-19 -0.3882740712677233 1106 20-21 -0.2804707605763994 1106 22-23 -0.34309092990954326 1106 24-25 -0.4345366081354243 1106 26-27 -0.2595673169390764 1106 28-29 -0.34481841338156727 1106 30-31 -0.3204275954913456 1106 32-33 -0.39484753982444687 1106 34-35 -0.38371194026460387 1106 36-37 -0.4921242638936931 1106 38-39 -0.6056386054979157 1106 40-41 -0.48469391262578654 1106 42-43 -0.6571597453274833 1106 44-45 -0.6147636537941565 1106 46-47 -0.5262263358271397 1106 48-49 -0.4452010610187074 1106 50-51 -0.5262327506878464 1106 52-53 -0.5601994175273362 1106 54-55 -0.44779832520936225 1106 56-57 -0.2852218510486253 1106 58-59 -0.5313453910145469 1106 60-61 -0.22397169249742177 1106 62-63 -0.12609760763709943 1106 64-65 -0.14447178647076697 1106 66-67 -0.17873329018359385 1106 68-69 -0.2974772030970456 1106 70-71 -0.26155679679823507 1106 72-73 0.09688499336731482 1106 74-75 0.24147915573573542 1106 76-77 0.10660771954862369 1106 78-79 0.0990083089651499 1106 80-81 0.06284593003550043 1106 82-83 -0.1452958206971502 1106 84-85 -0.13779189721848084 1106 86-87 0.05979490333867332 1106 88-89 0.07708594795582613 1106 90-91 0.1654069670014202 1106 92-93 0.2295140586165907 1106 94-95 0.3074378227951584 1106 96-97 0.16406510019680454 1106 98-99 0.1859846521345112 1106 100-101 0.880220774866924 1107 1 0.03825468782594754 1107 2 -0.13116704781249666 1107 3 -0.12729437260007614 1107 4 -0.08480107442971985 1107 5 -0.05540277720457709 1107 6 -0.161140647674614 1107 7 -0.1092112143726709 1107 8 -0.00105886727131832 1107 9 -0.13750350629896246 1107 10-11 -0.05965794351269693 1107 12-13 -0.10441473088162923 1107 14-15 -0.03756518505988282 1107 16-17 0.11561891157036541 1107 18-19 -0.05735632234996757 1107 20-21 0.07579134095842477 1107 22-23 -0.10328612865019693 1107 24-25 -0.024511421201147243 1107 26-27 -0.542283570382601 1107 28-29 -0.1429844897294572 1107 30-31 -0.22308324287426018 1107 32-33 -0.20557874801019693 1107 34-35 -0.4111184731256827 1107 36-37 -0.41706956102081705 1107 38-39 -0.34751581919331187 1107 40-41 -0.41471201573479277 1107 42-43 -0.387595007035479 1107 44-45 -0.6524488171156833 1107 46-47 -0.3194778116240755 1107 48-49 -0.40082719246301224 1107 50-51 -0.4846302319944229 1107 52-53 -0.37105183783430107 1107 54-55 -0.3383485023049886 1107 56-57 -0.22925018634968097 1107 58-59 -0.14916514701612726 1107 60-61 0.037810283102736264 1107 62-63 0.025948043681275124 1107 64-65 -0.11364612728146284 1107 66-67 -0.0640288431155085 1107 68-69 -0.10807224441936114 1107 70-71 -0.2069286976246829 1107 72-73 0.06797354118313237 1107 74-75 0.30035282751849834 1107 76-77 0.22518859322040896 1107 78-79 0.23887765174358222 1107 80-81 0.04982034955537529 1107 82-83 -0.029716127662911873 1107 84-85 0.20569413190391472 1107 86-87 0.26914948814780004 1107 88-89 0.3733881911671588 1107 90-91 0.3634872503544351 1107 92-93 0.6909678169794411 1107 94-95 0.4266751309415575 1107 96-97 0.5347742698150633 1107 98-99 0.8145678870813846 1107 100-101 1.3330354147724748 1108 1 -0.296283555800116 1108 2 -0.2889083131571617 1108 3 -0.19538880130640734 1108 4 -0.0818662302370825 1108 5 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-0.8082132573473295 2316 80-81 -0.9191493474143222 2316 82-83 -0.8234737034204898 2316 84-85 -1.1238210196112384 2316 86-87 -0.8932747542764368 2316 88-89 -0.9751572633782928 2316 90-91 -0.4679066890395056 2316 92-93 -0.3340927890811649 2316 94-95 -1.0132036569372325 2316 96-97 -0.4655590635182705 2316 98-99 -0.20785635534285873 2316 100-101 -0.17305549431843303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 13.0 5 27.0 6 59.0 7 136.0 8 291.0 9 573.0 10 949.0 11 1539.0 12 2309.0 13 3354.0 14 4148.0 15 5788.0 16 8112.0 17 11134.0 18 10004.0 19 5891.0 20 5027.0 21 5191.0 22 5087.0 23 5736.0 24 6327.0 25 7378.0 26 8697.0 27 10435.0 28 12496.0 29 15564.0 30 19356.0 31 23727.0 32 29518.0 33 38187.0 34 51011.0 35 72630.0 36 103983.0 37 143943.0 38 104960.0 39 8806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53672815007398 19.813268229504658 15.804983612470098 20.845020007951266 2 15.807698084353964 21.629459714086757 39.15960075915837 23.40324144240091 3 19.262551372902415 25.91515449419025 30.59094198446183 24.231352148445502 4 12.819399500266252 17.88104698316471 36.8486735209383 32.45087999563074 5 13.278415662050142 38.272919798626106 33.141490150456946 15.307174388866814 6 30.597495869686913 37.269351028823436 16.705034203088516 15.428118898401125 7 27.352230369065662 34.74992831688035 20.0725023553025 17.825338958751484 8 27.00036865604391 34.051939540408796 19.128879422165788 19.818812381381505 9 27.08106336787777 16.74394789661246 20.152377831483225 36.02261090402654 10-11 25.24662440315188 27.351243669689197 26.049817788088024 21.352314139070906 12-13 26.708413782556335 24.941625952760035 27.38009083138298 20.96986943330065 14-15 23.14183699941288 25.7698767050342 27.089870151148975 23.99841614440394 16-17 21.228102513688064 29.29893908982919 27.439820314313412 22.033138082169337 18-19 21.21861303403924 26.62795778205601 31.50623301792761 20.647196165977142 20-21 22.301164680020207 25.400947582572126 31.765043214680706 20.53284452272696 22-23 21.723143302850627 25.48483886493738 31.98123931142538 20.81077852078661 24-25 21.72653019983943 25.750421907513505 31.829371863001576 20.693676029645488 26-27 21.339835346950423 26.09755614677183 32.042897997442616 20.519710508835136 28-29 20.999936507199607 26.350263153762494 32.02795321741232 20.62184712162558 30-31 21.192591904837325 25.852348755031485 31.872859741011595 21.08219959911959 32-33 21.044388918472396 26.079479512281704 31.778355794044156 21.09777577520174 34-35 21.776580783462364 25.989773208263355 31.379251491691583 20.854394516582694 36-37 21.832167173341507 25.769826028363322 31.506049718080337 20.891957080214834 38-39 21.51204109846222 26.182246420098647 31.3459063678722 20.95980611356693 40-41 21.314614696141877 26.214466482657112 31.361386256902918 21.109532564298096 42-43 21.511870850391507 26.383248349715554 30.661083329464233 21.443797470428706 44-45 21.641641258948454 26.477049764537696 30.463933783822533 21.417375192691317 46-47 21.99285899589017 26.972924261663866 29.84891929163424 21.185297450811728 48-49 22.198691953740493 26.928071109654695 29.438413959775527 21.434822976829285 50-51 21.931757670093802 27.245866273433556 29.235789674899987 21.58658638157266 52-53 22.2899154070341 27.590988128584588 28.584380305834532 21.534716158546786 54-55 21.8592553147913 27.373393956771665 28.814361200999468 21.952989527437566 56-57 22.11315009762558 27.552192138068516 28.661846830240716 21.672810934065183 58-59 22.259588470623576 27.151449364409675 28.78288889799151 21.806073266975247 60-61 21.986305110665082 27.197053482434224 29.445991889567036 21.37064951733366 62-63 21.61696637037644 27.43599721459878 29.37417223064214 21.572864184382638 64-65 21.594164311364164 27.3343437239722 29.093788828356477 21.97770313630716 66-67 21.709539999180762 27.41135187536695 28.832179576455168 22.04692854899712 68-69 21.669056103988314 27.290378077253923 29.00879312934366 22.031772689414108 70-71 21.857138956020698 27.42780485806742 28.609961905542132 22.105094280369748 72-73 21.934898073430823 27.32649271562965 28.693046054697636 22.045563156241894 74-75 21.62379333415257 27.999289995767285 28.164229440598586 22.21268722948156 76-77 21.732683406381845 27.454634825707615 28.511312278977048 22.301369488933492 78-79 21.8444408033971 27.610357869441142 28.360231570611287 22.184969756550473 80-81 21.81067711284577 27.992706061942492 28.218405792550133 21.978211032661605 82-83 21.8476173441209 27.721565047963065 28.207647191749164 22.223170416166873 84-85 21.560414998323978 27.679844781833683 28.102502974855557 22.65723724498678 86-87 21.619424077335847 27.503515886344708 28.04434795668974 22.832712079629705 88-89 21.50582340010104 27.39517197121752 27.912451016534906 23.186553612146536 90-91 21.59454397855607 27.387163001303378 28.148843039701443 22.869449980439104 92-93 21.58440622145394 27.212989626813805 28.256111331855937 22.94649281987632 94-95 21.537568781660042 27.302530072775433 27.969934051529922 23.1899670940346 96-97 21.514834992285532 27.321099414246508 27.980788923933968 23.183276669533992 98-99 21.497455587126513 27.450029970411897 27.437604879374213 23.614909563087373 100-101 21.31869482394871 26.984357824160366 27.856591767204 23.840355584686918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 789.0 1 540.0 2 362.0 3 434.5 4 374.5 5 353.0 6 620.0 7 1430.0 8 2343.5 9 2639.0 10 2272.0 11 1852.0 12 1806.0 13 1917.5 14 1999.5 15 2054.0 16 2173.5 17 2281.5 18 2345.0 19 2380.5 20 2478.0 21 2692.0 22 2921.0 23 3304.5 24 3846.0 25 4527.5 26 5336.5 27 6341.5 28 7256.0 29 8239.0 30 9349.0 31 10388.0 32 11605.0 33 12600.0 34 13368.5 35 14184.0 36 15635.0 37 17200.0 38 18300.5 39 18646.0 40 19098.5 41 20449.5 42 21898.5 43 23221.5 44 23886.0 45 24846.0 46 24897.5 47 25913.0 48 29700.5 49 29647.5 50 26716.0 51 24988.0 52 23922.5 53 23653.5 54 23356.0 55 21794.0 56 19322.5 57 17273.0 58 15809.0 59 14114.0 60 12169.5 61 10338.5 62 8758.0 63 6912.0 64 5056.5 65 3865.0 66 2899.5 67 2430.5 68 1835.0 69 1129.0 70 830.0 71 640.5 72 530.5 73 450.5 74 377.5 75 297.5 76 198.0 77 121.5 78 76.5 79 58.0 80 34.0 81 27.0 82 26.0 83 11.0 84 5.5 85 5.5 86 2.5 87 2.0 88 1.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05884842775024236 2 0.0 3 0.0 4 0.0 5 0.0028673247859746856 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.3653927552260408E-4 12-13 0.006007728122994579 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010240445664195306 24-25 2.7307855104520817E-4 26-27 2.048089132839061E-4 28-29 0.003208672974781196 30-31 2.7307855104520817E-4 32-33 0.0 34-35 0.0 36-37 6.826963776130204E-5 38-39 3.413481888065102E-4 40-41 6.826963776130204E-5 42-43 0.012561613348079575 44-45 1.3653927552260408E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.826963776130204E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3653927552260408E-4 82-83 4.7788746432911425E-4 84-85 2.048089132839061E-4 86-87 0.0 88-89 0.0 90-91 0.008397165444640151 92-93 0.0028673247859746856 94-95 0.0 96-97 0.0 98-99 1.3653927552260408E-4 100-101 0.010240445664195305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 732390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.48306818710746 #Duplication Level Percentage of deduplicated Percentage of total 1 78.02438091574828 44.070564275210565 2 12.585361193085804 14.21719628856883 3 3.971194714316383 6.729157854990389 4 1.771662138640465 4.00275653525384 5 0.9568303353812634 2.7022356538416403 6 0.5944317561001747 2.0145197647432918 7 0.386869704057108 1.5296111511648602 8 0.28379970714968594 1.2823902568333472 9 0.1936953702618123 0.9846457925422473 >10 1.1068392934741917 11.382604734657159 >50 0.08119577869167692 3.134162898536293 >100 0.0405976112691067 4.366675001972494 >500 0.0014499146880409101 0.5443329553302204 >1k 0.0012082622400340919 1.3074637359850003 >5k 4.833048960136367E-4 1.731683100369736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7075 0.9660153743224239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5612 0.766258414232854 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4144 0.5658187577656713 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1814 0.2476822457980038 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1366 0.18651265036387718 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1208 0.16493944483130574 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.14295662147216648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 914 0.12479689782766014 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 735 0.100356367509114 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3653927552260408E-4 0.0 18-19 0.0 0.0 0.0 4.096178265678122E-4 0.0 20-21 0.0 0.0 0.0 4.096178265678122E-4 0.0 22-23 0.0 0.0 0.0 5.461571020904163E-4 0.0 24-25 0.0 0.0 0.0 6.826963776130204E-4 0.0 26-27 0.0 0.0 0.0 7.509660153743224E-4 0.0 28-29 0.0 0.0 0.0 0.0020480891328390613 0.0 30-31 0.0 0.0 0.0 0.004232717541200726 0.0 32-33 0.0 0.0 0.0 0.007509660153743224 0.0 34-35 0.0 0.0 0.0 0.010513524215240513 0.0 36-37 0.0 0.0 0.0 0.014541432843157334 0.0 38-39 0.0 0.0 0.0 0.019047228935403268 0.0 40-41 0.0 0.0 0.0 0.02512322669615915 0.0 42-43 0.0 0.0 0.0 0.038981963161703465 0.0 44-45 0.0 0.0 0.0 0.06198883108726225 0.0 46-47 0.0 0.0 0.0 0.08547358647715016 0.0 48-49 0.0 0.0 0.0 0.12643536913393139 0.0 50-51 0.0 0.0 0.0 0.17313180136266199 0.0 52-53 0.0 0.0 0.0 0.2307513756332009 0.0 54-55 0.0 0.0 0.0 0.3198432529117 0.0 56-57 0.0 0.0 0.0 0.41255342099154824 0.0 58-59 0.0 0.0 0.0 0.536599352803834 0.0 60-61 0.0 0.0 0.0 0.6680184054943404 0.0 62-63 0.0 0.0 0.0 0.8315241879326589 0.0 64-65 0.0 0.0 0.0 0.9810346946299102 0.0 66-67 0.0 0.0 0.0 1.169458894851104 0.0 68-69 0.0 0.0 0.0 1.4225344420322505 0.0 70-71 0.0 0.0 0.0 1.635126094020945 0.0 72-73 0.0 0.0 0.0 1.917898933628258 0.0 74-75 0.0 0.0 0.0 2.2546047870669996 0.0 76-77 0.0 0.0 0.0 2.544204590450443 0.0 78-79 0.0 0.0 0.0 2.8370813364464285 0.0 80-81 0.0 0.0 0.0 3.211608569204932 0.0 82-83 0.0 0.0 0.0 3.7006922541268996 0.0 84-85 0.0 0.0 0.0 4.223091522276382 0.0 86-87 0.0 0.0 0.0 4.776007318505169 0.0 88-89 0.0 0.0 0.0 5.430644874998293 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4735 0.0 33.316574 1 GTATCAA 6985 0.0 30.203606 1 TAAGGTG 370 0.0 26.957638 5 TGTACCG 90 8.947963E-4 26.387106 5 GTACATG 4485 0.0 24.36731 1 TATCAAC 8780 0.0 24.072973 2 ATCAACG 8750 0.0 24.046946 3 TCAACGC 8835 0.0 23.815594 4 CAACGCA 8885 0.0 23.735031 5 AGTACTT 5345 0.0 23.64216 12-13 GAGTACT 5285 0.0 23.551008 12-13 TACATGG 4575 0.0 23.359077 2 AACGCAG 9090 0.0 23.147503 6 ACATGGG 4480 0.0 22.900238 3 CATGGGG 1760 0.0 22.668924 4 GTACTTT 5550 0.0 22.293537 14-15 ATGGGGG 925 0.0 22.07959 5 AGAGTAC 8010 0.0 21.558826 10-11 TACTTTT 5880 0.0 21.405867 14-15 ACGCAGA 10155 0.0 20.630602 7 >>END_MODULE