##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937511_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252303 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.776467184298244 33.0 31.0 34.0 30.0 34.0 2 31.879838923833645 33.0 31.0 34.0 30.0 34.0 3 31.874377236893736 33.0 31.0 34.0 30.0 34.0 4 35.51490073443439 37.0 35.0 37.0 33.0 37.0 5 35.53626790010424 37.0 35.0 37.0 33.0 37.0 6 35.619873723261314 37.0 35.0 37.0 33.0 37.0 7 35.56604558804295 37.0 35.0 37.0 33.0 37.0 8 35.564729709912285 37.0 35.0 37.0 33.0 37.0 9 37.15141318176954 39.0 37.0 39.0 34.0 39.0 10-11 37.048622093276734 39.0 37.0 39.0 33.0 39.0 12-13 36.97201182704923 39.0 37.0 39.0 33.0 39.0 14-15 37.98746943159614 40.0 38.0 41.0 33.0 41.0 16-17 37.95982806387558 40.0 38.0 41.0 33.0 41.0 18-19 37.99814508745437 40.0 38.0 41.0 33.0 41.0 20-21 37.90668759388513 40.0 38.0 41.0 33.0 41.0 22-23 37.983309750577675 40.0 38.0 41.0 33.0 41.0 24-25 37.92387327935062 40.0 38.0 41.0 33.0 41.0 26-27 37.8255807501298 40.0 37.5 41.0 33.0 41.0 28-29 37.73695913247167 40.0 37.0 41.0 33.0 41.0 30-31 37.50471853287515 40.0 37.0 41.0 33.0 41.0 32-33 37.1690289057205 39.0 36.0 41.0 32.0 41.0 34-35 37.10109273373682 39.0 36.0 41.0 32.0 41.0 36-37 36.917468282184515 39.0 35.5 41.0 31.5 41.0 38-39 36.74559161008787 39.0 35.0 41.0 31.0 41.0 40-41 36.5932886251848 39.0 35.0 41.0 30.5 41.0 42-43 36.26788028679802 39.0 35.0 41.0 30.0 41.0 44-45 35.93054383023586 39.0 35.0 41.0 30.0 41.0 46-47 35.40849494457061 39.0 35.0 40.0 27.5 41.0 48-49 34.96067625038148 39.0 35.0 40.0 24.5 41.0 50-51 33.947461980238046 37.5 34.0 39.5 23.5 40.5 52-53 34.07493767414577 38.0 34.0 39.5 23.0 40.5 54-55 34.42785856688188 38.0 34.5 40.0 22.5 41.0 56-57 34.135539410946365 38.0 34.0 40.0 20.0 41.0 58-59 33.89124782503577 38.0 34.0 40.0 18.5 41.0 60-61 33.51431413815928 38.0 33.5 40.0 13.5 41.0 62-63 33.16297071378462 38.0 33.0 40.0 10.0 41.0 64-65 32.75019123831267 37.0 32.5 39.5 7.5 41.0 66-67 32.26496712286418 36.5 31.5 39.0 3.5 41.0 68-69 31.75696682163906 36.0 31.0 39.0 2.0 40.0 70-71 31.22059388909367 35.5 31.0 38.5 2.0 40.0 72-73 30.705958708378418 35.0 30.0 37.5 2.0 40.0 74-75 30.18384640689964 35.0 29.5 37.0 2.0 39.0 76-77 29.696999242973725 34.0 29.0 36.5 2.0 39.0 78-79 29.12896002029306 34.0 29.0 36.0 2.0 38.5 80-81 28.494669108175486 34.0 27.5 36.0 2.0 37.5 82-83 27.925642976896825 34.0 26.5 35.0 2.0 37.0 84-85 27.337738750629203 33.0 26.0 35.0 2.0 36.5 86-87 26.84684882859102 33.0 25.5 35.0 2.0 36.0 88-89 26.254560984213427 32.5 24.5 35.0 2.0 35.5 90-91 25.680439788666803 32.0 21.5 35.0 2.0 35.0 92-93 25.00363650055687 32.0 19.0 34.0 2.0 35.0 94-95 24.23548669655137 31.0 9.5 34.0 2.0 35.0 96-97 23.380025207785877 31.0 2.0 34.0 2.0 35.0 98-99 22.282489704839023 31.0 2.0 34.0 2.0 35.0 100-101 19.171519958145563 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15636197257427398 1101 2 0.05450993465508347 1101 3 -0.252080166763875 1101 4 -0.10369654448897592 1101 5 -0.28163473546355533 1101 6 -0.3931509333479113 1101 7 -0.01470919152485095 1101 8 -0.3821493841204813 1101 9 -0.07683700953644035 1101 10-11 -0.06270650961577218 1101 12-13 0.2913166650983072 1101 14-15 0.31019111343863415 1101 16-17 0.05723703745378117 1101 18-19 -0.0648947179378041 1101 20-21 0.2371936860832804 1101 22-23 0.3646162011781371 1101 24-25 0.3078061719362708 1101 26-27 0.08630468476221154 1101 28-29 0.12101954321160946 1101 30-31 -0.0705581690804351 1101 32-33 -0.16023248127814327 1101 34-35 0.10080156046755917 1101 36-37 0.27518616834004916 1101 38-39 0.4117012063461303 1101 40-41 0.3447213292564726 1101 42-43 -0.02323683166249424 1101 44-45 0.21020202594078086 1101 46-47 0.3213661614053436 1101 48-49 0.49404111823312746 1101 50-51 0.509898410453637 1101 52-53 0.5883777906457794 1101 54-55 0.5297837200173845 1101 56-57 0.4139037981421225 1101 58-59 0.455666343728673 1101 60-61 0.555123940342007 1101 62-63 0.6524795056032744 1101 64-65 0.4433528600351835 1101 66-67 1.0028501742055056 1101 68-69 0.8737026918079529 1101 70-71 0.4375764240968927 1101 72-73 0.3925836594154397 1101 74-75 0.18135091047270535 1101 76-77 0.44158787779205966 1101 78-79 0.6621780636641539 1101 80-81 0.9992399361232707 1101 82-83 0.6608147630920662 1101 84-85 1.128774943332953 1101 86-87 1.1065711188820266 1101 88-89 1.0185316926556034 1101 90-91 0.9218797358706041 1101 92-93 1.0800963626253797 1101 94-95 1.0676612361807862 1101 96-97 1.3990137774226987 1101 98-99 2.23360999219112 1101 100-101 2.1375614521012203 1102 1 0.004596931535314042 1102 2 0.0887565099975518 1102 3 0.07326229898955106 1102 4 -0.17903901024240554 1102 5 0.3553515659063109 1102 6 -0.050685179923256385 1102 7 -0.07635302714128755 1102 8 -0.05338226083281228 1102 9 0.19028627813479204 1102 10-11 -0.1363366466020821 1102 12-13 -0.13334086914827026 1102 14-15 -0.02028833861616164 1102 16-17 0.07949731142637972 1102 18-19 0.229625830007393 1102 20-21 0.0642484806038297 1102 22-23 0.21393126967128495 1102 24-25 0.22390206234722854 1102 26-27 0.27466084914577493 1102 28-29 0.4429373514307926 1102 30-31 0.6999897761250438 1102 32-33 0.9647675187218496 1102 34-35 0.720664574166193 1102 36-37 0.8830628806688097 1102 38-39 0.8586190145653134 1102 40-41 0.947461055283874 1102 42-43 0.8158042642279142 1102 44-45 0.9276677793654287 1102 46-47 1.1004757504464422 1102 48-49 1.06938358398655 1102 50-51 1.1794189583988413 1102 52-53 1.1928298454403077 1102 54-55 1.2917700213872436 1102 56-57 1.2752051680051366 1102 58-59 1.7501868916738772 1102 60-61 1.4986170910269436 1102 62-63 1.6096712864251899 1102 64-65 1.3731473805831271 1102 66-67 1.5028501742055056 1102 68-69 1.438771184958636 1102 70-71 1.7749051912201814 1102 72-73 1.719638453935982 1102 74-75 1.4844331022535293 1102 76-77 1.6881632202578132 1102 78-79 1.766630118458675 1102 80-81 1.88451390872601 1102 82-83 2.299513393229052 1102 84-85 2.358226998127474 1102 86-87 1.9901327627176393 1102 88-89 2.057915254299438 1102 90-91 1.8944824755966287 1102 92-93 1.9636580064609959 1102 94-95 2.0864968526191454 1102 96-97 2.1387398048199593 1102 98-99 1.9065551976705741 1102 100-101 1.9920135068957414 1103 1 0.26114338573342977 1103 2 0.26259864959311585 1103 3 0.26746600787036456 1103 4 0.2608314584930369 1103 5 0.15192224897405282 1103 6 0.2513835927913135 1103 7 0.16856822903241664 1103 8 0.05876944916625604 1103 9 0.27632206231147904 1103 10-11 -0.0168114392588663 1103 12-13 0.1909532320538574 1103 14-15 0.33038960379368376 1103 16-17 0.45012912579596076 1103 18-19 0.4168171448895137 1103 20-21 0.3552511830546692 1103 22-23 -0.0477754678182265 1103 24-25 -0.11746873254607237 1103 26-27 -0.07655292403472913 1103 28-29 -0.08900282189880926 1103 30-31 0.28196023554168903 1103 32-33 0.18818565309889124 1103 34-35 0.4514096119073159 1103 36-37 0.4849289695701273 1103 38-39 0.8547517142205194 1103 40-41 0.7671190554702463 1103 42-43 0.798564491606534 1103 44-45 0.9376971335728754 1103 46-47 1.0719951801361276 1103 48-49 1.0067845717789226 1103 50-51 1.0410930801023142 1103 52-53 0.9618518377988892 1103 54-55 1.3250847171285542 1103 56-57 1.2764632054666976 1103 58-59 1.4626787377273658 1103 60-61 1.7338700963386557 1103 62-63 1.6644774554681518 1103 64-65 1.3084050452928437 1103 66-67 1.532285921107004 1103 68-69 1.5706903909972176 1103 70-71 1.7023467157752101 1103 72-73 1.7274895395757248 1103 74-75 1.8227519914530461 1103 76-77 1.7120775805224646 1103 78-79 1.419598154988357 1103 80-81 1.5259965291714472 1103 82-83 1.544271570472553 1103 84-85 1.5552263458117537 1103 86-87 1.8648764958273212 1103 88-89 2.313809392776747 1103 90-91 2.051830346251741 1103 92-93 2.264655117634234 1103 94-95 2.476826272058151 1103 96-97 2.5073680780470617 1103 98-99 2.486906516280211 1103 100-101 2.5107209898889415 1104 1 0.26282733800286806 1104 2 0.16322316169303974 1104 3 0.2326829143050695 1104 4 0.06224776271964316 1104 5 0.17583214079964193 1104 6 0.4517850648555495 1104 7 0.21723556481919104 1104 8 0.154230584440036 1104 9 0.4834481914516573 1104 10-11 0.28796719350571465 1104 12-13 0.3198592206788149 1104 14-15 0.4867462448107389 1104 16-17 0.4312264827715424 1104 18-19 0.48199501931797784 1104 20-21 0.5113178720253373 1104 22-23 0.21599167838451905 1104 24-25 0.26698537696658065 1104 26-27 0.39869520800497327 1104 28-29 0.03625646214266709 1104 30-31 -0.02658219782509974 1104 32-33 0.2596922885399593 1104 34-35 0.20985725325893867 1104 36-37 0.2549189327685255 1104 38-39 0.46061766715907737 1104 40-41 0.7027608531729328 1104 42-43 0.5733609675739686 1104 44-45 0.41099924130120513 1104 46-47 0.024487652534922688 1104 48-49 -0.04004825971432524 1104 50-51 -0.09300974135413753 1104 52-53 0.08742337789033883 1104 54-55 0.24148931175329125 1104 56-57 -0.031666603834068496 1104 58-59 0.30698006481557627 1104 60-61 0.5955629702095138 1104 62-63 0.4686371521337023 1104 64-65 0.32979458471517376 1104 66-67 0.4889213621686821 1104 68-69 0.3669600464003615 1104 70-71 0.34897323523545154 1104 72-73 0.48194476428855637 1104 74-75 0.26730981458229763 1104 76-77 0.6404594250635611 1104 78-79 0.616046242420051 1104 80-81 0.7201640322382481 1104 82-83 0.9215883988432445 1104 84-85 1.0876229109952043 1104 86-87 1.1815598904966649 1104 88-89 1.0899273809556256 1104 90-91 1.160685035668493 1104 92-93 1.1692497423693737 1104 94-95 1.553446100317327 1104 96-97 1.4844730183838486 1104 98-99 1.8485269021394686 1104 100-101 1.2428331790268885 1105 1 0.06057390712765809 1105 2 -0.05514603785841388 1105 3 0.0332596217328458 1105 4 0.1855029557564336 1105 5 -0.027585384698305404 1105 6 -0.024425753748609225 1105 7 0.3163224447252091 1105 8 0.16256080284127705 1105 9 0.2968299397295482 1105 10-11 0.23680042894118003 1105 12-13 0.06883663297122666 1105 14-15 0.34207277869230523 1105 16-17 0.07540333971606117 1105 18-19 -0.12702938395003116 1105 20-21 0.08024508941200281 1105 22-23 0.3008584482889276 1105 24-25 0.34044081563801853 1105 26-27 0.4670830971489863 1105 28-29 0.16141265373769187 1105 30-31 0.20648770125109905 1105 32-33 0.37726584543641906 1105 34-35 0.36412649018956955 1105 36-37 0.23767110877108877 1105 38-39 0.16309003326148996 1105 40-41 0.038164281378200826 1105 42-43 -0.2607875879875152 1105 44-45 -0.10046014888408905 1105 46-47 -0.10815505585403429 1105 48-49 0.06358286112724443 1105 50-51 0.21603602144823242 1105 52-53 0.27896500228292354 1105 54-55 0.26720619112531807 1105 56-57 0.1523190852779095 1105 58-59 -0.13973323683444505 1105 60-61 0.33813786207687 1105 62-63 0.1425625005611053 1105 64-65 0.15406969551776228 1105 66-67 0.15075187426351278 1105 68-69 0.026334212043547467 1105 70-71 -0.03207017801813805 1105 72-73 0.39980667489890465 1105 74-75 0.20672349536405576 1105 76-77 0.42588688274498665 1105 78-79 0.5925080355529602 1105 80-81 0.8404395702315206 1105 82-83 0.5102358063297636 1105 84-85 0.4360236158598383 1105 86-87 0.2590576210461393 1105 88-89 0.31360442479607187 1105 90-91 0.838951709455003 1105 92-93 0.5978554987638844 1105 94-95 0.041468741423752675 1105 96-97 0.011135057151406613 1105 98-99 0.7092413785638847 1105 100-101 0.516443474322454 1106 1 -0.003810087294574771 1106 2 -0.8330375982170466 1106 3 -0.13631757914303932 1106 4 -0.2371436512833185 1106 5 0.06997107385517864 1106 6 -0.27199365108282336 1106 7 -0.10804542646322801 1106 8 -0.04534176053501682 1106 9 -0.04205211009995935 1106 10-11 -0.5360308327018615 1106 12-13 -0.39354611952624197 1106 14-15 -0.7281558879816217 1106 16-17 -0.3941477153752899 1106 18-19 -0.4045886749635059 1106 20-21 -0.39163185109544685 1106 22-23 -0.07886549580266689 1106 24-25 -0.16161816489425718 1106 26-27 -0.41061999567944696 1106 28-29 0.057096420473719434 1106 30-31 0.08325935050540068 1106 32-33 -0.007147998756501295 1106 34-35 0.19453294819835065 1106 36-37 0.2929966782589517 1106 38-39 0.42557736889710185 1106 40-41 0.2448793903734412 1106 42-43 0.1962734079489934 1106 44-45 0.35097950337698336 1106 46-47 0.782561011819972 1106 48-49 0.6072953446499483 1106 50-51 0.45504662138505125 1106 52-53 0.19474834088173054 1106 54-55 0.23083631542673544 1106 56-57 0.14241092234608743 1106 58-59 0.23314377993153812 1106 60-61 0.5055580357284555 1106 62-63 0.4563119150044699 1106 64-65 0.6653886414068779 1106 66-67 0.8165270423046493 1106 68-69 0.8660115294860624 1106 70-71 0.9675049529831377 1106 72-73 0.5459888456526656 1106 74-75 0.7432295470237698 1106 76-77 0.7445440788274738 1106 78-79 0.7788512207631619 1106 80-81 0.9934959035031206 1106 82-83 0.9796343443446425 1106 84-85 0.6231511746061464 1106 86-87 0.7555551753258776 1106 88-89 1.125022506784891 1106 90-91 1.5781311675836172 1106 92-93 1.9259524124719007 1106 94-95 1.6639181656717845 1106 96-97 1.4374638621801132 1106 98-99 1.7023127456615939 1106 100-101 0.8682676874977453 1107 1 0.11475851599606557 1107 2 0.2735819902978207 1107 3 0.2698143156145143 1107 4 0.35498490934705984 1107 5 0.37074662082805787 1107 6 0.27912100354508596 1107 7 0.3682481009811909 1107 8 0.21929681800853018 1107 9 0.15952797625600112 1107 10-11 0.3611134148933175 1107 12-13 0.3103378279974933 1107 14-15 0.32193885507313524 1107 16-17 0.5608260361390478 1107 18-19 0.5787924613318864 1107 20-21 0.472450694438173 1107 22-23 0.4667250692386986 1107 24-25 0.3024571325368015 1107 26-27 0.34902256932050335 1107 28-29 0.1842643797186767 1107 30-31 0.42998786764716357 1107 32-33 0.32735711115579846 1107 34-35 0.33910153078012684 1107 36-37 0.7002572061279082 1107 38-39 0.7823859257998933 1107 40-41 0.7978681972322192 1107 42-43 0.9208765743343648 1107 44-45 0.8098775847006934 1107 46-47 1.0539247940869743 1107 48-49 0.7287838978251386 1107 50-51 0.7565055608841007 1107 52-53 0.8002082329885454 1107 54-55 1.1302067659480812 1107 56-57 1.2449663962166824 1107 58-59 1.0460857847567056 1107 60-61 0.7789512867095425 1107 62-63 1.0632369592696733 1107 64-65 0.8513801300635961 1107 66-67 1.5234034207384397 1107 68-69 1.374238125880641 1107 70-71 1.3495213751126265 1107 72-73 1.571890457837764 1107 74-75 1.297031176811398 1107 76-77 1.611468916004025 1107 78-79 1.88528442949816 1107 80-81 1.8821495166822437 1107 82-83 1.769677522326976 1107 84-85 1.7118679498217766 1107 86-87 1.7005265453208018 1107 88-89 1.7726508240861136 1107 90-91 1.795517294715335 1107 92-93 2.1978494479979567 1107 94-95 2.173899838326765 1107 96-97 1.7125448856121928 1107 98-99 2.243740828952646 1107 100-101 1.3588865719112206 1108 1 0.03125794621560729 1108 2 0.33899076995917454 1108 3 0.2449860060976654 1108 4 0.20830425868803104 1108 5 0.1971703156274245 1108 6 0.1644993276846165 1108 7 0.23488921996950296 1108 8 0.12998751293899602 1108 9 0.31311658468068515 1108 10-11 0.3931522204721247 1108 12-13 0.41085617694516685 1108 14-15 0.5928871109398628 1108 16-17 0.2855010595857621 1108 18-19 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-0.4164250827254179 2316 54-55 -0.15243311927429914 2316 56-57 -0.6374243007552991 2316 58-59 -0.5348532018777377 2316 60-61 -0.6813862501023635 2316 62-63 -1.0594316203573015 2316 64-65 -0.17141493147835263 2316 66-67 -0.07558283615199457 2316 68-69 -0.39471528346769347 2316 70-71 -0.14119069919077631 2316 72-73 -0.5225934204375555 2316 74-75 -0.45037311224697163 2316 76-77 -0.6653282598624664 2316 78-79 -0.88426363362953 2316 80-81 -0.5340043004286841 2316 82-83 0.15091666753577115 2316 84-85 0.21042379064334327 2316 86-87 0.10490055423117184 2316 88-89 0.11337800070772275 2316 90-91 -0.35647809907761285 2316 92-93 0.1309650562438236 2316 94-95 0.778945900397293 2316 96-97 0.3728276765205969 2316 98-99 0.6480770820674984 2316 100-101 1.5743305800664729 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1111.0 3 179.0 4 143.0 5 160.0 6 158.0 7 191.0 8 283.0 9 339.0 10 427.0 11 634.0 12 988.0 13 1419.0 14 1620.0 15 2097.0 16 2928.0 17 4003.0 18 3253.0 19 2039.0 20 2048.0 21 2146.0 22 2289.0 23 2568.0 24 2752.0 25 3047.0 26 3477.0 27 3980.0 28 4701.0 29 5614.0 30 6710.0 31 8499.0 32 10532.0 33 13639.0 34 17916.0 35 24729.0 36 33761.0 37 45249.0 38 33302.0 39 3372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.82390599836969 23.262222398950236 16.611328707477583 19.3025428952025 2 14.097774244833069 24.706279809220984 38.181637519872815 23.014308426073132 3 18.866597248165842 28.39309418394843 33.07884298965876 19.661465578226966 4 10.738273704960772 19.793811155182144 42.651044854419865 26.816870285437215 5 9.907353309406561 40.96883557696512 36.62425825016594 12.499552863462386 6 24.964739629478068 41.90402027818942 17.44583808437856 15.685402007953945 7 22.079898289596535 36.41550289040307 24.93732493692763 16.567273883072765 8 22.74424887758751 33.076403512257144 22.526918034089555 21.652429576065796 9 22.739337396791086 19.185136327153383 25.67607301509095 32.399453260964584 10-11 21.750179822522124 28.383743249204212 29.355182266518838 20.51089466175483 12-13 24.798389900719815 27.291818573758796 29.119869348311124 18.78992217721026 14-15 21.57491045549991 29.489790039751202 29.788967005111438 19.146332499637452 16-17 17.37245039848077 32.26442754642321 30.758106680015572 19.605015375080452 18-19 16.99230349073936 31.680619693132726 33.067778936392074 18.259297879735836 20-21 18.765952966929063 29.152637987577297 32.330816613134225 19.750592432359422 22-23 18.53401872648761 28.487381271128875 33.36206793813983 19.616532064243692 24-25 20.2181353026821 29.625642319281596 31.865591152231904 18.2906312258044 26-27 18.48749677239955 28.354618944524994 34.88807674737323 18.269807535702228 28-29 17.50784695458699 30.951368747268464 33.390083038658666 18.150701259485874 30-31 18.527187075931487 29.151872905113958 32.57203825085583 19.748901768098722 32-33 18.19884895116287 29.931640333990906 33.57629721455717 18.293213500289056 34-35 19.589099332117556 29.32464808038428 32.43838675200941 18.64786583548875 36-37 18.564941540099557 28.585957705463805 33.75517955115191 19.09392120328472 38-39 17.95235399847072 30.43107814222302 33.11704948312331 18.49951837618296 40-41 17.79730695900858 30.664918970448046 33.1369161105815 18.40085795996187 42-43 17.720073255125673 30.0995538746956 31.124490014817873 21.055882855360853 44-45 18.713439838236493 28.716200782201245 31.813423749282453 20.75693563027981 46-47 20.569848449633195 30.06334960279859 31.501578277184805 17.865223670383415 48-49 19.294746357017274 29.65462437793649 31.533181687244845 19.5174475778014 50-51 16.9562176639354 30.304108021330872 32.99530307485675 19.744371239876973 52-53 18.563564458753152 31.69677457819114 30.14368330054254 19.595977662513167 54-55 18.74689584748827 29.306378362914664 31.09039689445683 20.856328895140237 56-57 20.15861067692558 29.44625290716727 29.78945256873427 20.605683847172873 58-59 20.683238571482477 28.79694484880139 30.768772478219336 19.75104410149679 60-61 19.605783170814053 30.35270986832959 31.783806327329057 18.257700633527296 62-63 18.139207874384812 30.556934773369136 32.011336508403076 19.292520843842972 64-65 18.544210283825972 31.230587749330752 30.790113355628463 19.43508861121482 66-67 19.163489037178266 29.848466793771845 29.35434540832539 21.6336987607245 68-69 18.36072020114155 29.23940372493178 31.391030453243722 21.008845620682944 70-71 20.771591146066076 29.018778363670666 30.23286759813706 19.976762892126196 72-73 19.127165552154977 27.958586638967724 32.786305706551424 20.12794210232588 74-75 18.137022214719114 31.59256795002929 30.24419309029881 20.02621674495278 76-77 18.213166953699343 29.178808446904913 30.984631336817454 21.62339326257829 78-79 18.686559692983675 28.97148736855069 31.473741920361682 20.868211018103953 80-81 20.123462675183525 29.307091556233516 29.84892903676868 20.720516731814282 82-83 19.15927665617335 28.484294779491353 31.848975660135654 20.507452904199646 84-85 18.607209593728093 28.984528743255776 31.086085797858924 21.322175865157206 86-87 18.49134840899297 29.94862466214807 30.778335375504668 20.781691553354293 88-89 18.812150581412666 28.966962255344924 29.716095846201206 22.5047913170412 90-91 19.461987607244996 27.18481887511916 30.860938989513826 22.49225452812202 92-93 20.850612517279185 28.607973052417496 30.766480766480765 19.77493366382255 94-95 19.510199379953963 27.56801738194399 31.30398342836601 21.61779980973603 96-97 18.07967394736999 28.24776504189864 32.34467512915188 21.327885881579498 98-99 19.360451604829883 29.24804697198723 29.612930008799303 21.77857141438358 100-101 19.40074712974677 27.0118583511907 31.038256141180113 22.549138377882414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 656.0 1 399.0 2 153.5 3 163.0 4 131.5 5 116.5 6 170.5 7 313.5 8 474.0 9 585.0 10 544.5 11 463.5 12 501.5 13 531.0 14 550.0 15 564.5 16 576.5 17 597.0 18 633.0 19 678.5 20 751.5 21 851.5 22 957.0 23 1107.5 24 1243.5 25 1485.0 26 2058.0 27 2685.5 28 2867.0 29 3207.5 30 4132.0 31 5042.5 32 6430.0 33 8127.0 34 9342.0 35 10549.5 36 12948.5 37 15240.5 38 14737.0 39 12908.5 40 11508.5 41 9825.0 42 7827.0 43 7412.5 44 7822.5 45 7603.5 46 7270.5 47 10008.0 48 16179.0 49 15261.5 50 8165.0 51 3956.5 52 2440.5 53 1891.0 54 1578.5 55 1395.5 56 1202.0 57 1050.5 58 936.0 59 816.5 60 688.5 61 551.5 62 445.0 63 324.0 64 220.0 65 165.0 66 124.5 67 102.5 68 81.0 69 63.5 70 45.0 71 38.0 72 30.5 73 25.0 74 21.0 75 20.0 76 20.0 77 13.0 78 7.0 79 6.0 80 5.0 81 3.0 82 3.5 83 2.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32421334665065416 2 0.2786332306789852 3 0.2730843469954777 4 0.2730843469954777 5 0.2782368818444489 6 0.23939469605989624 7 0.24098009139804122 8 0.2429618355707225 9 0.24930341692330255 10-11 0.2639683238011439 12-13 0.25425777735500565 14-15 0.24276366115345438 16-17 0.23662025421814248 18-19 0.22413526593025052 20-21 0.23245859145551184 22-23 0.22750423102380868 24-25 0.2292878007792218 26-27 0.22512613801659118 28-29 0.2429618355707225 30-31 0.25703221919675945 32-33 0.24553810299520815 34-35 0.24256548673618628 36-37 0.23463851004546118 38-39 0.21819003341220672 40-41 0.21521741715318488 42-43 0.22988232403102618 44-45 0.22889145194468558 46-47 0.23959287047716438 48-49 0.2459344518297444 50-51 0.25723039361402755 52-53 0.2806149748516664 54-55 0.24732167275062128 56-57 0.21977542875035178 58-59 0.21065940555601795 60-61 0.2132356729805036 62-63 0.21799185899493861 64-65 0.20966853346967734 66-67 0.21521741715318488 68-69 0.21402837064957608 70-71 0.21819003341220672 72-73 0.21739733574313425 74-75 0.220171777584888 76-77 0.23364763795912058 78-79 0.2338458123763887 80-81 0.22472978918205488 82-83 0.220171777584888 84-85 0.2035251265343654 86-87 0.2094703590524092 88-89 0.2162082892395255 90-91 0.21521741715318488 92-93 0.21997360316761988 94-95 0.21700098690859798 96-97 0.22373891709571428 98-99 0.2292878007792218 100-101 0.21383019623230798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 252303.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.96749146859134 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41561942101025 27.654843580932447 2 7.592676864914062 5.158083732654784 3 2.746759080990887 2.799015469495012 4 1.5460729746444033 2.100648823042136 5 0.9836524661322504 1.6706103375703023 6 0.7362807901891459 1.5005766875542503 7 0.5577531184000186 1.3261832003582992 8 0.43406728042846643 1.179534131579886 9 0.35005425841005355 1.0701418532478806 >10 2.981295434125623 20.985481742190938 >50 0.42473250020419834 10.089455931954832 >100 0.20419831740586458 11.290789249434212 >500 0.010501627752301608 2.766118516228503 >1k 0.016335865392469166 10.408516743756515 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4411 1.7482947091394079 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3341 1.3242014561856181 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2451 0.9714509934483537 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2273 0.9009009009009009 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1897 0.7518737391152701 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1545 0.6123589493585094 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1437 0.5695532752285941 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1397 0.5536993218471441 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1338 0.5303147406095052 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1317 0.521991415084244 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1258 0.4986068338466051 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1244 0.49305795016309756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 0.47324050843628496 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1158 0.4589719503929799 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 959 0.3800985323202657 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 938 0.3717752067950044 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 887 0.35156141623365555 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 805 0.3190608118016829 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 803 0.31826811413261036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 769 0.3047922537583778 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 685 0.2714989516573326 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 630 0.24969976575783878 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 503 0.19936346377173478 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 459 0.18192411505213968 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 458 0.18152776621760344 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 437 0.17320444069234214 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 415 0.1644847663325446 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 392 0.1553687431382108 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 0.1371366967495432 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 331 0.13119146423149944 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 322 0.12762432472067317 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 299 0.11850830152633936 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 297 0.11771560385726687 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 297 0.11771560385726687 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 293 0.11613020851912186 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 293 0.11613020851912186 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 280 0.11097767367015059 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 255 0.10106895280674429 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9817441726812602E-4 0.0 22-23 0.0 0.0 0.0 3.9634883453625204E-4 0.0 24-25 0.0 0.0 0.0 7.926976690725041E-4 0.0 26-27 0.0 0.0 0.0 0.00198174417268126 0.0 28-29 0.0 0.0 0.0 0.006341581352580033 0.0 30-31 0.0 0.0 0.0 0.012088639453355688 0.0 32-33 0.0 0.0 0.0 0.0198174417268126 0.0 34-35 0.0 0.0 0.0 0.02635719749666076 0.0 36-37 0.0 0.0 0.0 0.033689650935581426 0.0 38-39 0.0 0.0 0.0 0.041418453209038336 0.0 40-41 0.0 0.0 0.0 0.05806510425956092 0.0 42-43 0.0 0.0 0.0 0.07966611574178667 0.0 44-45 0.0 0.0 0.0 0.1280206735552094 0.0 46-47 0.0 0.0 0.0 0.23820564955628748 0.0 48-49 0.0 0.0 0.0 0.3141064513699797 0.0 50-51 0.0 0.0 0.0 0.40348311355790456 0.0 52-53 0.0 0.0 0.0 0.49186890365948877 0.0 54-55 0.0 0.0 0.0 0.6081972865958787 0.0 56-57 0.0 0.0 0.0 0.7526664367843426 0.0 58-59 0.0 0.0 0.0 0.9500481563833962 0.0 60-61 0.0 0.0 0.0 1.1890465036087563 0.0 62-63 0.0 0.0 0.0 1.4506367344026825 0.0 64-65 0.0 0.0 0.0 1.706281732678565 0.0 66-67 0.0 0.0 0.0 2.058834020998561 0.0 68-69 0.0 0.0 0.0 2.492439645981221 0.0 70-71 0.0 0.0 0.0 2.8550988295818915 0.0 72-73 0.0 0.0 0.0 3.340427977471532 0.0 74-75 0.0 0.0 0.0 3.813668485907817 0.0 76-77 0.0 0.0 0.0 4.2282493668327366 0.0 78-79 0.0 0.0 0.0 4.832483165083253 0.0 80-81 0.0 0.0 0.0 5.363986952196367 0.0 82-83 0.0 0.0 0.0 5.94007998319481 0.0 84-85 0.0 0.0 0.0 6.659056769043571 0.0 86-87 0.0 0.0 0.0 7.543311018893949 0.0 88-89 0.0 0.0 0.0 8.6546731509336 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 60 3.785317E-9 35.624256 94-95 TATATGG 75 3.0648804E-4 31.6786 2 TACGCTC 30 0.003949262 31.666006 42-43 CGCTCAA 35 0.008383952 27.144987 44-45 GTATTAT 125 7.305418E-6 26.62591 1 CTCGGTG 45 8.9416176E-4 26.388336 94-95 GACAATG 165 8.341158E-8 25.913702 7 GGACAAT 185 8.898496E-9 25.67514 6 CTATTCA 300 0.0 25.33784 9 GCAGATC 95 0.0012212052 25.00942 3 AATAGAC 195 1.5545083E-8 24.363308 7 TCTATTC 295 0.0 24.156841 8 CATGTAG 100 0.00164836 23.754225 8 GTATAGG 255 4.783942E-10 22.374716 1 CTATTCC 170 3.1279596E-6 22.356916 4 CAATGGG 215 4.3786713E-8 22.096954 9 GAATTAG 215 4.3786713E-8 22.096954 7 GTATTGG 305 9.094947E-12 21.824516 1 TTCTAGG 200 5.3017357E-7 21.39582 1 GATCTCG 90 5.1690513E-6 21.106472 92-93 >>END_MODULE