FastQCFastQC Report
Thu 26 May 2016
SRR937510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245568
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43031.7522641386499869No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33431.3613337242637478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.9142070628094866No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22270.9068771175397446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.7032675267135784No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15780.6425918686473807No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14320.5831378681261402No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13080.5326426896012509No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12890.5249055251498567No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12730.5183900182434193No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12500.5090239770654157No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12500.5090239770654157No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11730.4776681000781861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.4703381548084441No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC9250.3766777430284076No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA9000.36649726348709927No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8480.345321866041178No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC8180.33310529059160804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8110.3302547563200417No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT7060.2874967422465468No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG6400.26062027625749284No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC5960.2427026322647902No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG5280.21501172791243156No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC5200.2117539744592129No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.16940317956737036No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT3840.1563721657544957No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG3590.1461916862131874No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3470.14130505603335938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.14049061767005472No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA3260.13275345321866042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3180.12949569976544176No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3140.12786682303883243No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3010.12257297367735209No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG2950.1201296585874381No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA2880.11727912431587179No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT2740.11157805577273912No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG2660.10832030231952047No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC2570.10465532968464947No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA2500.10180479541308314No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCACG250.005013489657.0088279
AGATTGT250.005017548356.9971776
GTGTAGG502.7940816E-547.575431
TGTAGGG554.9293994E-543.215012
GTAAGCG250.001615301937.99811694-95
CATTCCG550.002573717634.5508089
GGATGAC1051.8943247E-631.66518
AGCGGGA300.003949710631.66509882-83
GATGACC1153.8435974E-628.9175229
CATGGGC1201.6942396E-423.7731174
ATAGGTG1000.001640618923.7731174
TATAGGT1000.001642574423.7682553
CATGGGG2653.092282E-1123.3245684
GAGTACT14750.022.86794912-13
GGGATGA1302.933338E-421.9219917
GTACTTT15150.021.7893514-15
GTACATG9250.021.601821
CATTTAC1553.842925E-521.485681
TAGGTCG1353.7697828E-421.1230185
TAGAGTA1150.003694136220.6722764
Produced by FastQC (version 0.11.5)