##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937510_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 245568 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.813583203023196 33.0 31.0 34.0 30.0 34.0 2 31.920588187385977 33.0 31.0 34.0 30.0 34.0 3 31.913563656502475 33.0 31.0 34.0 30.0 34.0 4 35.53968350925202 37.0 35.0 37.0 33.0 37.0 5 35.56732147511077 37.0 35.0 37.0 33.0 37.0 6 35.66092080401355 37.0 35.0 37.0 33.0 37.0 7 35.61396028798541 37.0 35.0 37.0 33.0 37.0 8 35.637289874902265 37.0 35.0 37.0 33.0 37.0 9 37.225689014855355 39.0 37.0 39.0 34.0 39.0 10-11 37.11540184388845 39.0 37.0 39.0 33.5 39.0 12-13 37.04498957518895 39.0 37.0 39.0 33.0 39.0 14-15 38.14373819064373 40.0 38.0 41.0 33.0 41.0 16-17 38.1530207518895 40.0 38.0 41.0 33.0 41.0 18-19 38.23025190578577 40.0 38.0 41.0 33.5 41.0 20-21 38.1998224524368 40.0 38.0 41.0 34.0 41.0 22-23 38.05006352619233 40.0 38.0 41.0 33.5 41.0 24-25 37.86948218008861 40.0 37.5 41.0 33.0 41.0 26-27 37.711747866171486 40.0 37.0 41.0 33.0 41.0 28-29 37.79064454652072 40.0 37.0 41.0 33.0 41.0 30-31 37.6600208496221 40.0 37.0 41.0 33.0 41.0 32-33 37.364815448266874 39.5 36.0 41.0 33.0 41.0 34-35 37.19929103140474 39.5 36.0 41.0 32.5 41.0 36-37 37.1358198136565 40.0 36.0 41.0 32.5 41.0 38-39 37.003913376335674 40.0 36.0 41.0 32.0 41.0 40-41 36.89992995830076 40.0 36.0 41.0 31.5 41.0 42-43 36.58915453153506 39.5 36.0 41.0 31.0 41.0 44-45 36.28614477456346 39.0 35.0 41.0 30.0 41.0 46-47 35.83140107831639 39.0 35.0 41.0 29.0 41.0 48-49 35.41012469051343 39.0 35.0 41.0 27.0 41.0 50-51 34.47176953023195 38.0 34.0 40.0 24.5 40.5 52-53 34.59754935496481 38.5 35.0 40.0 24.0 40.5 54-55 34.95120496155851 39.0 35.0 40.0 24.0 41.0 56-57 34.72790428720354 39.0 35.0 40.5 23.0 41.0 58-59 34.50537732929372 39.0 35.0 40.0 22.0 41.0 60-61 34.163472032186604 38.0 34.0 40.0 19.5 41.0 62-63 33.8638849524368 38.0 34.0 40.0 18.0 41.0 64-65 33.497530215663275 38.0 34.0 40.0 13.0 41.0 66-67 33.084180349231175 37.0 33.0 40.0 8.0 41.0 68-69 32.59754935496481 36.5 32.5 39.0 6.5 41.0 70-71 32.08333129723742 36.0 32.0 39.0 2.0 40.0 72-73 31.658398081183215 36.0 31.5 38.5 2.0 40.0 74-75 31.224892901355226 35.0 31.0 37.5 2.0 40.0 76-77 30.82874397315611 35.0 31.0 37.0 2.0 39.0 78-79 30.344686604117797 35.0 31.0 36.5 2.0 39.0 80-81 29.809136369559553 34.5 30.0 36.0 2.0 38.5 82-83 29.319003290330986 34.0 29.5 35.5 2.0 37.0 84-85 28.85803728498827 34.0 29.0 35.0 2.0 37.0 86-87 28.56501661454261 34.0 29.0 35.0 2.0 36.0 88-89 28.127201019676832 34.0 28.5 35.0 2.0 36.0 90-91 27.714445693249935 34.0 27.0 35.0 2.0 36.0 92-93 27.298055935626792 34.0 26.5 35.0 2.0 35.0 94-95 26.796750798149596 33.5 25.5 35.0 2.0 35.0 96-97 26.238101055512118 33.0 25.0 35.0 2.0 35.0 98-99 25.626180935626792 33.0 22.0 35.0 2.0 35.0 100-101 23.744362050430023 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12998170228841133 1101 2 -0.0878123553549699 1101 3 0.15978372349738024 1101 4 -0.07499020504118903 1101 5 0.07062199841785599 1101 6 0.16254645641411258 1101 7 0.06909348952183336 1101 8 0.2628684164168149 1101 9 0.030408351122403587 1101 10-11 0.05121850996938804 1101 12-13 0.008036760608419513 1101 14-15 0.22544436091630615 1101 16-17 0.32455290314658214 1101 18-19 0.2942999708682805 1101 20-21 0.2526497204264686 1101 22-23 0.3468863500894699 1101 24-25 0.3415905010632372 1101 26-27 0.3558701642729005 1101 28-29 0.10832054200975705 1101 30-31 0.43110812787592323 1101 32-33 0.2510215166908125 1101 34-35 0.22227718700587218 1101 36-37 0.5469104698053115 1101 38-39 0.6731155610886148 1101 40-41 0.503027712653072 1101 42-43 0.6275668335948694 1101 44-45 0.6771509708341057 1101 46-47 0.9611261830288456 1101 48-49 1.033039834290058 1101 50-51 0.8822349520012693 1101 52-53 0.7282270541212768 1101 54-55 0.8770883991946121 1101 56-57 1.1302992955400342 1101 58-59 1.364017246686707 1101 60-61 1.3402498412807873 1101 62-63 1.3179445580413542 1101 64-65 1.2915701764328986 1101 66-67 1.5062780393219057 1101 68-69 1.519768644201072 1101 70-71 1.271378614721133 1101 72-73 1.0834200862528256 1101 74-75 1.2132318605902235 1101 76-77 1.2016284625374531 1101 78-79 1.3908258333594041 1101 80-81 1.6386111016108842 1101 82-83 1.2052322127389665 1101 84-85 1.442129655416064 1101 86-87 1.4002722312153857 1101 88-89 1.2424549654501966 1101 90-91 1.4992964472073282 1101 92-93 1.4294167516292866 1101 94-95 1.7864504595973933 1101 96-97 1.6254270728649338 1101 98-99 1.1462051637150417 1101 100-101 1.5528187028005682 1102 1 -0.054272294540854915 1102 2 -0.2700565564617641 1102 3 -0.18723146548177994 1102 4 -0.38652911944128476 1102 5 -0.21486016336243097 1102 6 -0.1174723826298063 1102 7 -0.047390673906875236 1102 8 -0.07085347219734217 1102 9 -0.20452630094400348 1102 10-11 -0.037648792515014406 1102 12-13 0.11145133731864831 1102 14-15 0.023513358914662774 1102 16-17 0.1378579779140381 1102 18-19 -0.11166965117340055 1102 20-21 -0.24604332090168413 1102 22-23 -0.1402795512410293 1102 24-25 -0.1075676291616503 1102 26-27 -0.09051509417523818 1102 28-29 -0.06524003729084171 1102 30-31 -0.22165885669960517 1102 32-33 0.22057879915872292 1102 34-35 -0.1597962852654149 1102 36-37 0.09310733781426705 1102 38-39 0.17004244204940733 1102 40-41 0.17488689079978315 1102 42-43 0.3360679521631056 1102 44-45 0.3994163658653065 1102 46-47 0.7076703959100428 1102 48-49 0.6348884154745704 1102 50-51 0.8574203988398352 1102 52-53 0.9638563959321829 1102 54-55 0.8038398415589043 1102 56-57 0.6096823168517034 1102 58-59 0.7353230279183052 1102 60-61 1.0166833747789568 1102 62-63 0.6922586990987014 1102 64-65 0.7679154019127026 1102 66-67 1.1181760729966967 1102 68-69 0.8348339921346692 1102 70-71 0.9480652978719597 1102 72-73 0.9940347100606637 1102 74-75 1.1315999284107825 1102 76-77 1.099927061383564 1102 78-79 1.1946701757590255 1102 80-81 0.8766836319299678 1102 82-83 0.7330315769212312 1102 84-85 0.7481816982748875 1102 86-87 0.19203603670225888 1102 88-89 0.4853432263709543 1102 90-91 0.3877280968011121 1102 92-93 0.8488857260788336 1102 94-95 0.5792680741034602 1102 96-97 0.38663041679523147 1102 98-99 0.5595808848971906 1102 100-101 0.8479028897780765 1103 1 0.2852041723660683 1103 2 0.0130958637458356 1103 3 0.10569673754374875 1103 4 0.17042074999914547 1103 5 -0.2771187360549803 1103 6 -0.08790765313629123 1103 7 -0.2698922384637257 1103 8 0.10242389675753572 1103 9 -0.08541496923636771 1103 10-11 0.10599371163915805 1103 12-13 0.05187948639393625 1103 14-15 0.09569877508678104 1103 16-17 0.12593497569129397 1103 18-19 -0.03836207008620107 1103 20-21 -0.2962925709933941 1103 22-23 -0.3953994286660958 1103 24-25 -0.08991511184362366 1103 26-27 0.22549841496393697 1103 28-29 -0.06994989292001463 1103 30-31 -0.17515914803087895 1103 32-33 -0.1224314082259852 1103 34-35 0.01901098291041592 1103 36-37 0.003609290812811139 1103 38-39 0.1350718988784294 1103 40-41 0.03495076787956464 1103 42-43 0.05929244988640647 1103 44-45 0.12721584362702032 1103 46-47 0.49308872604232334 1103 48-49 0.5915663757240353 1103 50-51 0.5694070623414262 1103 52-53 0.5688543458231976 1103 54-55 0.07755097041365389 1103 56-57 0.2740686868639983 1103 58-59 0.2781595155421215 1103 60-61 0.2523218218753627 1103 62-63 0.21373628817054424 1103 64-65 0.35717067285078485 1103 66-67 0.42650706848645825 1103 68-69 0.7328842305947063 1103 70-71 0.6710739946500546 1103 72-73 0.7063342849328045 1103 74-75 0.9410369037447381 1103 76-77 1.2720013400689005 1103 78-79 0.9136272097908353 1103 80-81 0.9951745359201283 1103 82-83 0.8163846920078761 1103 84-85 0.9128917697050021 1103 86-87 0.7086041542706134 1103 88-89 0.8767360056713294 1103 90-91 0.8642651390333569 1103 92-93 0.8956659759763284 1103 94-95 1.0212391999369892 1103 96-97 1.0970579184676872 1103 98-99 1.6039927194210257 1103 100-101 1.4639768231819055 1104 1 -0.054333867104119804 1104 2 -0.1193269215870636 1104 3 -0.21124476515544544 1104 4 0.02634570353759358 1104 5 -0.3700024986045136 1104 6 -0.03545772835975214 1104 7 -0.19129601635295046 1104 8 -0.015386854788722815 1104 9 0.07100065757661156 1104 10-11 -0.18361168478355694 1104 12-13 0.20653989918881166 1104 14-15 0.09974019223773212 1104 16-17 0.20277598378396533 1104 18-19 0.2406382937396785 1104 20-21 0.11159065073388774 1104 22-23 -0.07851200819154514 1104 24-25 0.2480447099800216 1104 26-27 -0.008269542792930906 1104 28-29 -0.06676395823167525 1104 30-31 -0.01444152607546556 1104 32-33 0.2511341836793832 1104 34-35 0.3134247807608972 1104 36-37 0.3478279009979701 1104 38-39 0.37895814921029825 1104 40-41 0.6873348106733559 1104 42-43 0.44927937182117006 1104 44-45 0.3215578596156945 1104 46-47 0.49153017465929594 1104 48-49 0.8517264591089955 1104 50-51 0.6888501137588676 1104 52-53 0.813886156004429 1104 54-55 0.7344680869461016 1104 56-57 1.078536861933081 1104 58-59 1.2272878084121146 1104 60-61 0.8228991250406423 1104 62-63 0.6410251954146062 1104 64-65 0.8897675046157332 1104 66-67 1.129664487092704 1104 68-69 1.2227462717562005 1104 70-71 0.7882947582910695 1104 72-73 0.949040559454172 1104 74-75 0.9563028407930254 1104 76-77 0.8891385220900041 1104 78-79 1.2047321588060456 1104 80-81 1.39013210595661 1104 82-83 1.049606910952388 1104 84-85 1.2251330354257206 1104 86-87 1.2343620429005675 1104 88-89 0.8931229197395893 1104 90-91 0.5510749760983629 1104 92-93 0.16067379331607157 1104 94-95 0.567538500801323 1104 96-97 0.6357119593931806 1104 98-99 0.7542528057559217 1104 100-101 1.2057882827136517 1105 1 -0.04920097232846388 1105 2 -0.02227391117377664 1105 3 0.04781892403892485 1105 4 -0.16719982877899042 1105 5 -0.5214917264086836 1105 6 -0.26154109365076295 1105 7 -0.21201442495890888 1105 8 -0.010116296168511951 1105 9 -0.2944181846704552 1105 10-11 -0.08854005041872881 1105 12-13 -0.27448707309158493 1105 14-15 -0.02005685192286677 1105 16-17 0.005356197556245945 1105 18-19 -0.36633634661353653 1105 20-21 -0.0824662044339135 1105 22-23 -0.24588174377863226 1105 24-25 -0.1686932468918627 1105 26-27 0.04382638924046489 1105 28-29 0.19371570193529664 1105 30-31 0.16246143074724984 1105 32-33 -0.21085584552501757 1105 34-35 0.34216210831235117 1105 36-37 0.0647025384012423 1105 38-39 -0.392259290832186 1105 40-41 -0.41842543790821907 1105 42-43 -0.10951783950265792 1105 44-45 -0.20075843290031514 1105 46-47 -0.1612521099705333 1105 48-49 -0.1029673863338374 1105 50-51 -0.08654149071323758 1105 52-53 -0.1867726638230991 1105 54-55 -0.15235256656384166 1105 56-57 -0.07159047712313793 1105 58-59 -0.16235495391125454 1105 60-61 -0.017774641147148884 1105 62-63 0.1397813042477125 1105 64-65 -0.09620553293699174 1105 66-67 -0.22301061640100528 1105 68-69 -0.08705146072362169 1105 70-71 0.09068814256002256 1105 72-73 -0.12485542567813113 1105 74-75 -0.1956190448083177 1105 76-77 -0.03346489787321971 1105 78-79 0.08886836734710712 1105 80-81 -0.019294917456075922 1105 82-83 0.04628822898536811 1105 84-85 0.23604289510693022 1105 86-87 0.11053341472077349 1105 88-89 0.2529417934526812 1105 90-91 0.7083165859786966 1105 92-93 0.7557945933396688 1105 94-95 0.6852263382006534 1105 96-97 0.1308199763455029 1105 98-99 0.37087374835993714 1105 100-101 1.0379318071047337 1106 1 -0.16958069978214496 1106 2 0.06206233136182249 1106 3 -0.2622714143472322 1106 4 -0.18443047237619226 1106 5 -0.042494125224585844 1106 6 -0.2568353313887286 1106 7 0.0370132890205781 1106 8 -0.06545238558820188 1106 9 -0.042440437515857354 1106 10-11 -0.5564673714007071 1106 12-13 -0.24885546996802788 1106 14-15 -0.36118888887483536 1106 16-17 -0.5918213658592606 1106 18-19 -0.1107961026488482 1106 20-21 -0.2582943063070289 1106 22-23 -0.36355642351119144 1106 24-25 -0.07244458665606146 1106 26-27 0.23348085767305804 1106 28-29 0.06262296473322948 1106 30-31 0.07012233004802937 1106 32-33 0.06539077316533337 1106 34-35 -0.17839115050458076 1106 36-37 -0.22451810162325359 1106 38-39 -0.3075861932973467 1106 40-41 -0.1897876591096619 1106 42-43 -0.06449665846861308 1106 44-45 0.1840014303161439 1106 46-47 0.9383191654849838 1106 48-49 0.8745603021263193 1106 50-51 0.7758022034632504 1106 52-53 0.46657291878292995 1106 54-55 0.2563699363708807 1106 56-57 0.5066568561081155 1106 58-59 0.5510682074218707 1106 60-61 0.44167005849048024 1106 62-63 0.30875491727277193 1106 64-65 0.5505509617294706 1106 66-67 0.5812153826803055 1106 68-69 0.3802531888961411 1106 70-71 0.5848289071187942 1106 72-73 0.9601953577649347 1106 74-75 0.6819035397882232 1106 76-77 0.940893291275966 1106 78-79 0.8657631767177989 1106 80-81 0.6183103497311855 1106 82-83 0.49437172819427033 1106 84-85 0.49075120930077176 1106 86-87 0.26409846346267685 1106 88-89 -0.017027072979207247 1106 90-91 -0.14280881595056982 1106 92-93 0.15848943333354626 1106 94-95 0.18285814547876456 1106 96-97 0.9459784513110527 1106 98-99 1.1485443450015929 1106 100-101 1.2755421781556215 1107 1 -0.27595985521790567 1107 2 -0.402807390111672 1107 3 -0.04160654462576119 1107 4 -0.06184886111533672 1107 5 -0.17936145077115384 1107 6 -0.11266042872325954 1107 7 -0.18284295536396655 1107 8 -0.28135535054776994 1107 9 -0.03135927549129747 1107 10-11 -0.3521247538505463 1107 12-13 -0.12392285541276493 1107 14-15 -0.2599909254127155 1107 16-17 -0.32190522330161286 1107 18-19 -0.22367420986658004 1107 20-21 0.02075630764924341 1107 22-23 -0.1935803827804392 1107 24-25 -0.43974018414033367 1107 26-27 -0.06726786949075603 1107 28-29 -0.17307634643777448 1107 30-31 -0.06521759208434474 1107 32-33 -0.027975504163208598 1107 34-35 0.09230366830345105 1107 36-37 0.22489789118895942 1107 38-39 0.08706789475296972 1107 40-41 0.3847556173204012 1107 42-43 0.23542846881500168 1107 44-45 0.6085147576596626 1107 46-47 1.0785707378120293 1107 48-49 1.0164890233003305 1107 50-51 0.46060304203767544 1107 52-53 0.8207957399868917 1107 54-55 0.40325523136938557 1107 56-57 0.6965190903099767 1107 58-59 0.908869944468897 1107 60-61 0.6344074050014115 1107 62-63 0.7711388645623813 1107 64-65 0.6825665352544803 1107 66-67 0.8786547357800387 1107 68-69 0.9356739735622099 1107 70-71 1.3666947352110377 1107 72-73 1.149937795620584 1107 74-75 1.1427750582069045 1107 76-77 1.290009793222655 1107 78-79 0.9713786304464591 1107 80-81 1.2785978609620905 1107 82-83 1.1025178797366486 1107 84-85 1.3249830152306963 1107 86-87 1.1959193679250895 1107 88-89 1.0990256374131242 1107 90-91 1.2993295488293093 1107 92-93 1.479019532165534 1107 94-95 1.6779764443706462 1107 96-97 1.607568747807008 1107 98-99 1.5912068187961417 1107 100-101 1.7725472695003326 1108 1 -0.4405465155755479 1108 2 -0.2130832127492397 1108 3 -0.18527484769549574 1108 4 -0.04135439136907593 1108 5 -0.23381621309839318 1108 6 -0.2741911556411054 1108 7 -0.37224182368281333 1108 8 -0.363914842671349 1108 9 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-1.7291434015773817 2316 50-51 -1.6427163150552673 2316 52-53 -1.4524747002646876 2316 54-55 -1.9108258308248836 2316 56-57 -1.5679463184950535 2316 58-59 -1.4293550730014104 2316 60-61 -1.2318749150544974 2316 62-63 -1.5192874107695573 2316 64-65 -1.8039463927678874 2316 66-67 -2.575398373933453 2316 68-69 -2.6747732661303125 2316 70-71 -2.781837759547873 2316 72-73 -2.286365488795912 2316 74-75 -2.565715507830827 2316 76-77 -3.0305352801526055 2316 78-79 -2.187146876192255 2316 80-81 -3.102324570903736 2316 82-83 -3.390284356461816 2316 84-85 -3.0732699547203914 2316 86-87 -2.7406634412992297 2316 88-89 -2.7672916232437608 2316 90-91 -2.8205865937283434 2316 92-93 -2.7393941645500526 2316 94-95 -2.9827503201297 2316 96-97 -2.1624871571545583 2316 98-99 -2.1070112105539636 2316 100-101 -3.3196959170824734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 917.0 3 173.0 4 150.0 5 142.0 6 154.0 7 174.0 8 212.0 9 308.0 10 409.0 11 591.0 12 896.0 13 1280.0 14 1456.0 15 1917.0 16 2651.0 17 3651.0 18 3034.0 19 1765.0 20 1749.0 21 1690.0 22 1837.0 23 1991.0 24 2107.0 25 2336.0 26 2609.0 27 2839.0 28 3411.0 29 4046.0 30 4875.0 31 6117.0 32 8103.0 33 10413.0 34 14542.0 35 21123.0 36 31426.0 37 49091.0 38 48191.0 39 7192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.621195159221095 23.415756228703106 16.53660410044371 19.426444511632088 2 13.994241725032161 24.606211585975945 38.33745124864721 23.06209544034468 3 18.995033978044955 28.185850104547832 33.003626502875065 19.81548941453215 4 10.658901273039898 19.66697570319424 42.51634694319309 27.157776080572766 5 9.868047586181548 40.812542626224676 36.82497416901834 12.494435618575435 6 24.946847365220833 41.885092369281246 17.53404421156585 15.63401605393207 7 21.97976171046189 36.449730700179536 25.079973886078015 16.490533703280562 8 22.97603891228414 32.854274801684454 22.625110175301145 21.544576110730258 9 22.884212072497 19.315668785650754 25.679149359816556 32.120969782035694 10-11 21.863917037714213 28.43686614128239 29.376558501716136 20.322658319287264 12-13 24.89318724367999 27.31876517516476 29.073473301911818 18.714574279243436 14-15 21.473429064600243 29.50000204030833 29.806864413350148 19.21970448174128 16-17 17.410864575363426 32.17607753124203 30.63973476154042 19.773323131854116 18-19 17.04685718016358 31.71015344313668 32.85857973799804 18.3844096387017 20-21 18.776673439578285 29.31283507552204 32.260479652704674 19.650011832194995 22-23 18.511634928860254 28.618701572133293 33.29062636434485 19.5790371346616 24-25 20.238629502097037 29.74627511137947 31.698679765654326 18.31641562086917 26-27 18.457116512855663 28.408525291098545 34.914689970869745 18.219668225176047 28-29 17.50744685191986 31.008283347614967 33.32007181621577 18.164197984249398 30-31 18.677984431272126 29.133517655100643 32.37417998918555 19.814317924441678 32-33 18.178720591205774 29.950399598463225 33.514446550517526 18.356433259813475 34-35 19.645109026131173 29.345026996363753 32.39073920655585 18.61912477094922 36-37 18.510562338708855 28.58757407917419 33.834980670563155 19.0668829115538 38-39 18.01500587505712 30.585384163457146 32.99354559697108 18.406064364514656 40-41 17.685744067592623 30.705932509368033 33.16579326888752 18.442530154151818 42-43 17.82139031978375 30.258785127760497 30.85265466415056 21.067169888305198 44-45 18.651578116297713 28.977932281051793 31.59517150067425 20.775318101976243 46-47 20.507730412819143 30.277144174545334 31.44231671638015 17.77280869625537 48-49 19.277949169972086 29.737361866052918 31.557791815614642 19.426897148360347 50-51 17.03181336810338 30.386041377171342 32.945435105095726 19.63671014962955 52-53 18.61247280578946 31.75304187398213 30.14098131813859 19.49350400208982 54-55 18.61296443655506 29.5213319458897 30.975087225316766 20.890616392238474 56-57 20.16857290308894 29.67038122688599 29.633256023156335 20.527789846868732 58-59 20.6472827262083 29.001660431549013 30.648629022058856 19.70242782018383 60-61 19.596624668800217 30.508726948596976 31.748877643224148 18.145770739378662 62-63 18.190831145464227 30.640367358012906 31.845174346138233 19.323627150384638 64-65 18.329461151374097 31.385275645641297 30.844122068581477 19.441141134403132 66-67 19.128538705209717 29.901051897270207 29.288516023228635 21.681893374291445 68-69 18.32437239432483 29.336129037521726 31.507559007579406 20.831939560574046 70-71 20.626770863293945 29.081425543676705 30.37429598940899 19.91750760362036 72-73 19.133750010199336 28.01716752205096 32.78515303083463 20.063929436915068 74-75 18.105541026279177 31.62824001729913 30.336109082452396 19.930109873969293 76-77 18.283746511514043 29.26941719843977 30.958782824408793 21.488053465637392 78-79 18.60449935129048 29.22599570790936 31.399172589370956 20.770332351429204 80-81 20.057119543043655 29.53814769481844 29.79885760913913 20.605875152998777 82-83 19.04005221985374 28.69990922720736 31.92285332544596 20.337185227492938 84-85 18.552459818879008 29.136207881210733 31.079587174675694 21.23174512523456 86-87 18.518193685241087 30.042424736885042 30.822591172391288 20.616790405482583 88-89 18.656019255089138 29.13229714845184 29.65508097744054 22.556602619018477 90-91 19.257813217142264 27.335091702415408 31.02664683911739 22.380448241324938 92-93 20.79009797182214 28.924023392480873 30.767598925430857 19.518279710266135 94-95 19.29001517549728 27.747295334758416 31.394513976143468 21.568175513600835 96-97 17.877023181813545 28.44886844601279 32.50063742338171 21.173470948791955 98-99 19.14946566224632 29.46476471392175 29.780532930259696 21.60523669357224 100-101 18.72770402158771 27.870661398917758 31.010302297263394 22.39133228223114 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 601.0 1 357.0 2 136.0 3 153.5 4 123.5 5 118.0 6 181.5 7 332.5 8 507.0 9 590.5 10 536.0 11 492.0 12 520.5 13 529.0 14 548.0 15 549.5 16 564.0 17 580.0 18 590.5 19 659.0 20 765.0 21 902.5 22 999.0 23 1099.5 24 1241.0 25 1541.0 26 2072.5 27 2637.0 28 2787.5 29 3147.5 30 4119.5 31 4988.0 32 6259.0 33 7940.0 34 9150.5 35 10298.5 36 12770.0 37 14835.0 38 14046.5 39 12340.5 40 11100.5 41 9442.5 42 7432.5 43 7021.5 44 7577.0 45 7453.0 46 7061.5 47 9877.5 48 16353.5 49 15145.5 50 7636.0 51 3649.0 52 2252.0 53 1740.5 54 1502.0 55 1319.5 56 1118.5 57 972.5 58 908.0 59 823.0 60 667.5 61 531.0 62 427.5 63 303.5 64 194.0 65 149.0 66 120.5 67 93.0 68 75.5 69 54.0 70 37.5 71 35.0 72 35.5 73 29.0 74 22.5 75 23.5 76 22.0 77 14.0 78 9.0 79 7.5 80 5.5 81 3.0 82 2.0 83 2.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3314764138649987 2 0.2862750847015898 3 0.2866823038832421 4 0.2936050299713318 5 0.28790396142819913 6 0.2113467552775606 7 0.19872296064633832 8 0.2052384675527756 9 0.19627964555642427 10-11 0.2203055772739119 12-13 0.20890344018764662 14-15 0.2064601250977326 16-17 0.2058492963252541 18-19 0.20096266614542613 20-21 0.1930218921032056 22-23 0.19831574146468595 24-25 0.18732082356007299 26-27 0.18813526192337765 28-29 0.20279515246286162 30-31 0.21399367995830076 32-33 0.20747817305186342 34-35 0.2178622621839979 36-37 0.19363272087568412 38-39 0.18894970028668231 40-41 0.18426667969768049 42-43 0.1956688167839458 44-45 0.19607603596559814 46-47 0.20238793328120927 48-49 0.2101250977326036 50-51 0.2156225566849101 52-53 0.2325221527234819 54-55 0.20890344018764662 56-57 0.18385946051602814 58-59 0.18365585092520198 60-61 0.17775117279124317 62-63 0.18996774824081314 64-65 0.18060170706280948 66-67 0.17897283033620015 68-69 0.17510424811050299 70-71 0.18508111806098515 72-73 0.18487750847015896 74-75 0.19098579619494396 76-77 0.19383633046651028 78-79 0.18976413864998698 80-81 0.19057857701329164 82-83 0.18345224133437582 84-85 0.17428980974719832 86-87 0.17428980974719832 88-89 0.17836200156372167 90-91 0.18508111806098515 92-93 0.1818233646077665 94-95 0.177547563200417 96-97 0.17938004951785247 98-99 0.18385946051602814 100-101 0.1744934193380245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 245568.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.15673051863435 #Duplication Level Percentage of deduplicated Percentage of total 1 81.37056200672406 27.793523586135 2 7.509716493001741 5.130147250456085 3 2.8267245284818427 2.8965500390930417 4 1.5701375807720737 2.1452306489444877 5 1.0503350103722073 1.793800495178525 6 0.6902882758291805 1.4146794370602032 7 0.590142826486087 1.4110144644253322 8 0.45780776842557047 1.2509773260359656 9 0.38031426595770046 1.1691262705238468 >10 2.919716731443287 21.00680870471723 >50 0.40058179737237415 9.471918165233255 >100 0.20505972960728674 11.141109590826167 >500 0.01192207730274923 2.969442272608809 >1k 0.016690908223848923 10.405671748762053 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4303 1.7522641386499869 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3343 1.3613337242637478 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.9142070628094866 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2227 0.9068771175397446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1727 0.7032675267135784 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1578 0.6425918686473807 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1432 0.5831378681261402 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1308 0.5326426896012509 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1289 0.5249055251498567 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1273 0.5183900182434193 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1250 0.5090239770654157 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1250 0.5090239770654157 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1173 0.4776681000781861 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.4703381548084441 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 925 0.3766777430284076 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 900 0.36649726348709927 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 848 0.345321866041178 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 818 0.33310529059160804 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 811 0.3302547563200417 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 706 0.2874967422465468 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 640 0.26062027625749284 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 596 0.2427026322647902 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 528 0.21501172791243156 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 520 0.2117539744592129 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.16940317956737036 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 384 0.1563721657544957 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 359 0.1461916862131874 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 347 0.14130505603335938 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.14049061767005472 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 326 0.13275345321866042 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 318 0.12949569976544176 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 314 0.12786682303883243 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 301 0.12257297367735209 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 295 0.1201296585874381 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 288 0.11727912431587179 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 274 0.11157805577273912 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 266 0.10832030231952047 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 257 0.10465532968464947 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 250 0.10180479541308314 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0360959082616628E-4 0.0 12-13 0.0 0.0 0.0 4.0721918165233256E-4 0.0 14-15 0.0 0.0 0.0 4.0721918165233256E-4 0.0 16-17 0.0 0.0 0.0 6.108287724784988E-4 0.0 18-19 0.0 0.0 0.0 8.144383633046651E-4 0.0 20-21 0.0 0.0 0.0 0.0012216575449569976 0.0 22-23 0.0 0.0 0.0 0.0024433150899139952 0.0 24-25 0.0 0.0 0.0 0.0032577534532186605 0.0 26-27 0.0 0.0 0.0 0.004479410998175658 0.0 28-29 0.0 0.0 0.0 0.007533554860568152 0.0 30-31 0.0 0.0 0.0 0.014456280948657807 0.0 32-33 0.0 0.0 0.0 0.020360959082616627 0.0 34-35 0.0 0.0 0.0 0.02463676048996612 0.0 36-37 0.0 0.0 0.0 0.031152267396403442 0.0 38-39 0.0 0.0 0.0 0.03726055512118843 0.0 40-41 0.0 0.0 0.0 0.0488663017982799 0.0 42-43 0.0 0.0 0.0 0.06983808965337503 0.0 44-45 0.0 0.0 0.0 0.10974556945530363 0.0 46-47 0.0 0.0 0.0 0.1920038441490748 0.0 48-49 0.0 0.0 0.0 0.2471820432629659 0.0 50-51 0.0 0.0 0.0 0.31620569455303627 0.0 52-53 0.0 0.0 0.0 0.4092552775605942 0.0 54-55 0.0 0.0 0.0 0.5137069976544175 0.0 56-57 0.0 0.0 0.0 0.6301716836069846 0.0 58-59 0.0 0.0 0.0 0.80568315089914 0.0 60-61 0.0 0.0 0.0 1.0101071800886108 0.0 62-63 0.0 0.0 0.0 1.2467015246286162 0.0 64-65 0.0 0.0 0.0 1.482685040396143 0.0 66-67 0.0 0.0 0.0 1.8078495569455302 0.0 68-69 0.0 0.0 0.0 2.211607375553818 0.0 70-71 0.0 0.0 0.0 2.5324960906958562 0.0 72-73 0.0 0.0 0.0 2.997744005733646 0.0 74-75 0.0 0.0 0.0 3.435097406828251 0.0 76-77 0.0 0.0 0.0 3.8364119103466248 0.0 78-79 0.0 0.0 0.0 4.378217031535053 0.0 80-81 0.0 0.0 0.0 4.877671357831639 0.0 82-83 0.0 0.0 0.0 5.485445986447745 0.0 84-85 0.0 0.0 0.0 6.218644123012771 0.0 86-87 0.0 0.0 0.0 7.124910411780037 0.0 88-89 0.0 0.0 0.0 8.27510099035705 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCACG 25 0.0050134896 57.008827 9 AGATTGT 25 0.0050175483 56.997177 6 GTGTAGG 50 2.7940816E-5 47.57543 1 TGTAGGG 55 4.9293994E-5 43.21501 2 GTAAGCG 25 0.0016153019 37.998116 94-95 CATTCCG 55 0.0025737176 34.550808 9 GGATGAC 105 1.8943247E-6 31.6651 8 AGCGGGA 30 0.0039497106 31.665098 82-83 GATGACC 115 3.8435974E-6 28.917522 9 CATGGGC 120 1.6942396E-4 23.773117 4 ATAGGTG 100 0.0016406189 23.773117 4 TATAGGT 100 0.0016425744 23.768255 3 CATGGGG 265 3.092282E-11 23.324568 4 GAGTACT 1475 0.0 22.867949 12-13 GGGATGA 130 2.933338E-4 21.921991 7 GTACTTT 1515 0.0 21.78935 14-15 GTACATG 925 0.0 21.60182 1 CATTTAC 155 3.842925E-5 21.48568 1 TAGGTCG 135 3.7697828E-4 21.123018 5 TAGAGTA 115 0.0036941362 20.672276 4 >>END_MODULE