##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937510_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 245568 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08174517852489 31.0 31.0 34.0 30.0 34.0 2 32.32281078967944 34.0 31.0 34.0 31.0 34.0 3 32.372858027104506 34.0 31.0 34.0 30.0 34.0 4 36.01007053036226 37.0 35.0 37.0 35.0 37.0 5 35.85476120667188 37.0 35.0 37.0 35.0 37.0 6 35.969601088089654 37.0 35.0 37.0 35.0 37.0 7 35.93278032968465 37.0 35.0 37.0 35.0 37.0 8 35.93701948136565 37.0 35.0 37.0 35.0 37.0 9 37.58953935366171 39.0 37.0 39.0 35.0 39.0 10-11 37.51889497002867 39.0 37.0 39.0 35.0 39.0 12-13 37.487323266875165 39.0 37.0 39.0 35.0 39.0 14-15 38.58033212796455 40.0 38.0 41.0 34.0 41.0 16-17 38.586424126922076 40.0 38.0 41.0 34.0 41.0 18-19 38.68254210646339 40.0 38.0 41.0 34.5 41.0 20-21 38.62954252997133 40.0 38.0 41.0 34.0 41.0 22-23 38.501751042481104 40.0 38.0 41.0 34.0 41.0 24-25 38.33529002150117 40.0 38.0 41.0 34.0 41.0 26-27 38.081460125097735 40.0 37.0 41.0 33.5 41.0 28-29 38.10176610959083 40.0 37.0 41.0 33.5 41.0 30-31 38.19819968399791 40.0 37.0 41.0 34.0 41.0 32-33 38.04842650508209 40.0 37.0 41.0 34.0 41.0 34-35 37.867501058769875 40.0 37.0 41.0 33.0 41.0 36-37 37.690287415298414 40.0 36.5 41.0 33.0 41.0 38-39 37.510675250847015 40.0 36.0 41.0 33.0 41.0 40-41 37.40929599947876 40.0 36.5 41.0 33.0 41.0 42-43 37.14774115519938 40.0 36.0 41.0 32.5 41.0 44-45 36.803716282251756 40.0 36.0 41.0 31.5 41.0 46-47 36.35523968921032 40.0 36.0 41.0 30.5 41.0 48-49 35.94563013096169 39.0 35.5 41.0 29.5 41.0 50-51 35.685871123273394 39.0 35.0 41.0 28.0 41.0 52-53 35.5198621155851 39.0 35.0 41.0 27.5 41.0 54-55 35.355461623664326 39.0 35.0 41.0 27.0 41.0 56-57 35.073816621058114 39.0 35.0 40.0 26.0 41.0 58-59 34.824431522022415 39.0 35.0 40.0 25.0 41.0 60-61 34.43360902071932 38.0 34.0 40.0 23.0 41.0 62-63 34.115564731561115 38.0 34.0 40.0 22.0 41.0 64-65 33.76576549061767 37.5 34.0 40.0 21.5 41.0 66-67 33.33118321605421 37.0 33.5 39.5 19.5 41.0 68-69 32.84845338154808 36.5 33.0 39.0 16.5 40.0 70-71 32.36241489119104 36.0 32.0 39.0 12.0 40.0 72-73 31.883115878290333 35.5 32.0 38.0 8.0 40.0 74-75 31.456126205368776 35.0 31.0 37.0 6.5 39.5 76-77 30.102051163017983 34.0 30.0 36.0 2.0 38.5 78-79 30.709679192728693 35.0 31.0 36.0 2.0 39.0 80-81 30.465349719833203 35.0 31.0 36.0 2.0 38.5 82-83 30.11108328446703 34.0 31.0 35.5 2.0 37.0 84-85 29.731351397576233 34.0 30.5 35.0 2.0 37.0 86-87 29.512145312092784 34.0 30.0 35.0 2.0 36.0 88-89 29.10618036551994 34.0 30.0 35.0 2.0 36.0 90-91 28.687760620276258 34.0 29.0 35.0 2.0 36.0 92-93 28.31235950938233 34.0 29.0 35.0 2.0 35.0 94-95 27.933038913864998 34.0 29.0 35.0 2.0 35.0 96-97 27.544452045869168 34.0 28.5 35.0 2.0 35.0 98-99 27.024756890148552 33.5 27.0 35.0 2.0 35.0 100-101 25.475153521631483 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11871986806448831 1101 2 0.10553193866380894 1101 3 0.1980938038427027 1101 4 0.24587435112858458 1101 5 -0.2076174613610462 1101 6 -0.18567117378767506 1101 7 0.12803759993152397 1101 8 0.10021747560492855 1101 9 0.2478012207844671 1101 10-11 0.06987246470030328 1101 12-13 0.21871135386530938 1101 14-15 0.21414276733606386 1101 16-17 0.013179535361963701 1101 18-19 0.3073998281900998 1101 20-21 -0.0010662266443048907 1101 22-23 0.10719884116068812 1101 24-25 0.3197747888914009 1101 26-27 0.01485615110961902 1101 28-29 -0.08006800488446686 1101 30-31 0.042033424679104314 1101 32-33 -0.11197188867620866 1101 34-35 -0.04888488815556258 1101 36-37 0.11338665082683974 1101 38-39 0.5670580056825756 1101 40-41 0.4281832385051061 1101 42-43 0.21083966244034258 1101 44-45 0.13811165728565555 1101 46-47 0.2509543486552488 1101 48-49 0.1748562274768446 1101 50-51 0.12524624713528 1101 52-53 0.2045953422560629 1101 54-55 0.08485797334488865 1101 56-57 0.3791083200767602 1101 58-59 0.24122732300094896 1101 60-61 0.6225938505946544 1101 62-63 0.5595342453240377 1101 64-65 0.5873826042861054 1101 66-67 0.5701879958653961 1101 68-69 0.3615548608697239 1101 70-71 0.6446081131703849 1101 72-73 0.5388438982532229 1101 74-75 0.6617491341259587 1101 76-77 0.8399240779330412 1101 78-79 0.7712476582026007 1101 80-81 0.511455828503685 1101 82-83 0.46668253454193476 1101 84-85 0.354810691878658 1101 86-87 0.07383249785708301 1101 88-89 0.11684975535569819 1101 90-91 0.6764746091510361 1101 92-93 0.5126003493747966 1101 94-95 0.37646152983534265 1101 96-97 0.045610870702599726 1101 98-99 0.20498824827218343 1101 100-101 0.19794356303064475 1102 1 0.04989848574964384 1102 2 -0.019912545028645923 1102 3 -0.08737658353513922 1102 4 0.01337700036913958 1102 5 0.033133745515200985 1102 6 -0.008548837746232607 1102 7 0.14511048348268218 1102 8 0.011591548370731175 1102 9 0.003470595563697998 1102 10-11 0.06757056902151248 1102 12-13 -0.047107555825029124 1102 14-15 -0.2278624134010201 1102 16-17 -0.40477642837287675 1102 18-19 -0.1689571716921563 1102 20-21 -0.05515783149536446 1102 22-23 -0.052367860829185986 1102 24-25 -0.014753646290515121 1102 26-27 -0.05982299642364808 1102 28-29 -0.19721742205154413 1102 30-31 -0.09245968729546661 1102 32-33 -0.31345546338479124 1102 34-35 -0.3294335753096931 1102 36-37 0.09115069180176505 1102 38-39 -0.40975819589519347 1102 40-41 -0.5410502478954626 1102 42-43 -0.3585351167896178 1102 44-45 -0.33547835802107073 1102 46-47 -0.6884133659332363 1102 48-49 -0.986971159783252 1102 50-51 -1.0626967647568648 1102 52-53 -0.6886402635369464 1102 54-55 -0.5130285214154995 1102 56-57 -0.45199965119370233 1102 58-59 -0.18385804141680495 1102 60-61 -0.16108682761093007 1102 62-63 -0.34702998404132046 1102 64-65 -0.11946421073803748 1102 66-67 -0.32038659497411714 1102 68-69 -0.3380919070567998 1102 70-71 -0.20807645058800617 1102 72-73 -0.06192143790596205 1102 74-75 -0.3946561408063367 1102 76-77 -0.48361295256266246 1102 78-79 0.06637894278990686 1102 80-81 -0.052243688745814865 1102 82-83 0.21290883855700926 1102 84-85 0.110032639364821 1102 86-87 0.2723842463558448 1102 88-89 0.451825617830675 1102 90-91 0.28071928123392453 1102 92-93 0.3215430080348689 1102 94-95 0.127898006934128 1102 96-97 0.1238105645681351 1102 98-99 0.3315983978070953 1102 100-101 0.35446069478620856 1103 1 0.1315251932746193 1103 2 0.10950328181267821 1103 3 0.11404122867487843 1103 4 0.11864470144166717 1103 5 0.0061694174114421685 1103 6 -0.026298465489603018 1103 7 -0.05467803159101692 1103 8 -0.08248687369275842 1103 9 -0.19149476838459378 1103 10-11 0.10886947491071908 1103 12-13 0.040486047834953354 1103 14-15 0.31242222804571185 1103 16-17 0.06108386209519523 1103 18-19 -0.018673393449212483 1103 20-21 0.06408298077939634 1103 22-23 -0.07640808612536887 1103 24-25 0.11919939542380575 1103 26-27 -0.18292125059399922 1103 28-29 0.05234018601077395 1103 30-31 0.11226527439986711 1103 32-33 0.3218409392134518 1103 34-35 0.4938578207066229 1103 36-37 0.4807415332096454 1103 38-39 0.4796546097328971 1103 40-41 0.3746039526699363 1103 42-43 0.35602497298358315 1103 44-45 0.39129606525090566 1103 46-47 0.7176322975467642 1103 48-49 0.933862263467141 1103 50-51 1.0415998320622322 1103 52-53 1.291835145945349 1103 54-55 1.371544948016286 1103 56-57 1.3217438478028285 1103 58-59 1.2963691969785032 1103 60-61 1.2959903643871797 1103 62-63 1.333449741459873 1103 64-65 1.4482163607028653 1103 66-67 1.5532026063465807 1103 68-69 1.3422961030426919 1103 70-71 1.2934827112411185 1103 72-73 1.5434865337809534 1103 74-75 1.669409764802328 1103 76-77 1.2954608297805024 1103 78-79 1.8320137212702363 1103 80-81 1.3395429272795631 1103 82-83 1.848518152525802 1103 84-85 1.943596160373044 1103 86-87 1.6424397071987649 1103 88-89 1.1703726300665913 1103 90-91 1.500043176872591 1103 92-93 1.5692878881574472 1103 94-95 1.7956131065262646 1103 96-97 1.988077165055845 1103 98-99 2.0376559303390884 1103 100-101 2.224580895912691 1104 1 -0.0895531079535381 1104 2 -0.17700467810747966 1104 3 -0.1469890868703203 1104 4 0.06969537156960115 1104 5 0.028854452368229033 1104 6 0.05588684971143465 1104 7 -0.17455374080564212 1104 8 0.022766968603477267 1104 9 -0.06695815471851319 1104 10-11 -0.03511707336504344 1104 12-13 -0.017583511739069024 1104 14-15 0.18811669297584643 1104 16-17 0.010089240818267342 1104 18-19 -0.025482837188938845 1104 20-21 -0.21278359345583198 1104 22-23 -0.14206882341358806 1104 24-25 -0.1758633891224406 1104 26-27 -0.11374516870368012 1104 28-29 -0.1957448115801057 1104 30-31 0.09418214510402123 1104 32-33 0.21400589983176133 1104 34-35 0.11734720584089331 1104 36-37 0.42924050564737826 1104 38-39 0.09826676151711666 1104 40-41 0.30011306306650454 1104 42-43 0.4295423825433531 1104 44-45 0.7651517345430179 1104 46-47 0.9182970172533444 1104 48-49 1.1228845551768316 1104 50-51 0.637526948889672 1104 52-53 0.8920249253882062 1104 54-55 0.7981848685645829 1104 56-57 0.8187349094860608 1104 58-59 0.7791317170135059 1104 60-61 0.9940247213495184 1104 62-63 1.1382079939156995 1104 64-65 0.8956877708722928 1104 66-67 1.1558310024644598 1104 68-69 1.043093570027942 1104 70-71 0.8972075015495875 1104 72-73 0.7989758795827022 1104 74-75 0.9559226412883568 1104 76-77 0.836656735264441 1104 78-79 1.0066894737506438 1104 80-81 0.8377877132614522 1104 82-83 0.9694509234040005 1104 84-85 0.9476965763145166 1104 86-87 1.0466797536111478 1104 88-89 1.0030078110453786 1104 90-91 1.0525570170055332 1104 92-93 0.9911292404097267 1104 94-95 0.7649534017168804 1104 96-97 0.7610301528529284 1104 98-99 1.0302417489964704 1104 100-101 1.0673786185593777 1105 1 0.0736473797697954 1105 2 0.09342611139467039 1105 3 0.08510232192247003 1105 4 0.04147428343523529 1105 5 -0.4793611292123501 1105 6 -0.16456213108445894 1105 7 -0.08040150425982517 1105 8 0.04613048965129707 1105 9 -0.06930827320774569 1105 10-11 -0.03421734180953706 1105 12-13 -0.19102520618433516 1105 14-15 -0.010728897356216294 1105 16-17 -0.06271035012344583 1105 18-19 -0.07172805582862907 1105 20-21 -0.12401991311128313 1105 22-23 0.0763250328456877 1105 24-25 -0.023887421296450384 1105 26-27 0.08395977834490509 1105 28-29 -0.10682380112749001 1105 30-31 0.006155949641026837 1105 32-33 -0.046326263358899666 1105 34-35 0.27520830302173493 1105 36-37 0.41964828562122136 1105 38-39 0.32370605667823327 1105 40-41 0.41640459575675237 1105 42-43 0.08432079291926442 1105 44-45 0.1565693771480099 1105 46-47 0.093838085328116 1105 48-49 0.06247962681118935 1105 50-51 0.0223072275606242 1105 52-53 0.3095200334051569 1105 54-55 0.3377828901384774 1105 56-57 0.26065060021439734 1105 58-59 0.06968109408460776 1105 60-61 -0.023945326931155364 1105 62-63 0.21126324628302484 1105 64-65 0.38873083015624843 1105 66-67 0.6062572687260044 1105 68-69 0.485382919762948 1105 70-71 -0.028060697119009603 1105 72-73 0.004901324777737415 1105 74-75 0.25944191914316406 1105 76-77 0.02375664971619429 1105 78-79 0.5281552003699161 1105 80-81 0.5147840848358349 1105 82-83 0.29867892422998565 1105 84-85 0.11722960088750867 1105 86-87 0.13922427311516472 1105 88-89 0.2989899934106397 1105 90-91 0.2556701865188913 1105 92-93 0.18343579812529143 1105 94-95 0.2602433316066488 1105 96-97 0.23888102357674867 1105 98-99 0.28685207181818484 1105 100-101 0.21332687662008354 1106 1 -0.04886576267903564 1106 2 0.0978460572937152 1106 3 -0.15846425797851538 1106 4 -0.03215405321560638 1106 5 0.027136089181972523 1106 6 0.06963375520146542 1106 7 -0.05324894977608352 1106 8 0.0972099568079372 1106 9 -0.1933015361077608 1106 10-11 -0.08167306579260014 1106 12-13 -0.07101295691163756 1106 14-15 -0.06388563700813421 1106 16-17 0.07324636911300786 1106 18-19 0.04424193345325733 1106 20-21 -0.12086572539117668 1106 22-23 -0.14860734096128425 1106 24-25 0.002147387053469174 1106 26-27 0.15804746272198145 1106 28-29 0.21533717473123914 1106 30-31 0.3305881448127721 1106 32-33 -0.06368450354671751 1106 34-35 0.18820784367400734 1106 36-37 0.15666150462801198 1106 38-39 -0.00980080801834049 1106 40-41 -0.21893455598111444 1106 42-43 -0.09433995326559597 1106 44-45 -0.3212032967661429 1106 46-47 -0.148544398211925 1106 48-49 -0.217875602097088 1106 50-51 -0.2799333685706529 1106 52-53 -0.22105210970718048 1106 54-55 -0.381223898000151 1106 56-57 -0.5187195830143025 1106 58-59 -0.6168344982187577 1106 60-61 -0.17205944932180728 1106 62-63 -0.4596804581011895 1106 64-65 -0.4082732018960229 1106 66-67 -0.12413113529584052 1106 68-69 -0.017893760684152937 1106 70-71 -0.2172966487343757 1106 72-73 -0.505684088380022 1106 74-75 -0.7486936394747126 1106 76-77 -0.6244034074470761 1106 78-79 -0.36868550804635447 1106 80-81 -0.7501983068346618 1106 82-83 -0.7720810410637462 1106 84-85 -0.8497841285056396 1106 86-87 -0.5558249791688148 1106 88-89 -0.444887922171457 1106 90-91 -0.34624886620401796 1106 92-93 -0.5402818561389857 1106 94-95 -0.5536136581345836 1106 96-97 -0.5041802738253871 1106 98-99 -0.3261729881522122 1106 100-101 0.3337831620281406 1107 1 -0.37614938052869107 1107 2 -0.19215094779762865 1107 3 -0.2168350276700437 1107 4 -0.16084527813324456 1107 5 -0.25465486619388855 1107 6 -0.10685621185454153 1107 7 -0.23459066885364876 1107 8 -0.2521161887446297 1107 9 -0.2787462800430731 1107 10-11 -0.260349316166959 1107 12-13 -0.3532681231290624 1107 14-15 -0.6583001323660298 1107 16-17 -0.19259669767345144 1107 18-19 -0.07745617975428587 1107 20-21 -0.1853760578260406 1107 22-23 -0.10643902709485786 1107 24-25 -0.19141355933766135 1107 26-27 -0.38352186941339284 1107 28-29 -0.2594456943912533 1107 30-31 -0.5391483032255664 1107 32-33 -0.5933191246907725 1107 34-35 -0.3716753548884313 1107 36-37 -0.14512046602188633 1107 38-39 -0.20411048157329503 1107 40-41 -0.46089322624167295 1107 42-43 -0.1322255477549632 1107 44-45 -0.2683431590003451 1107 46-47 -0.7249145689217187 1107 48-49 -0.9463122597790274 1107 50-51 -0.4741082712361191 1107 52-53 -0.5414324631063963 1107 54-55 -0.8555425562810655 1107 56-57 -0.8537450397378734 1107 58-59 -0.5429501016928597 1107 60-61 -0.5731525909809818 1107 62-63 -0.4368742286911953 1107 64-65 -0.3352423543368701 1107 66-67 -0.13903080585588867 1107 68-69 -0.2443537786536112 1107 70-71 -0.05067490569754085 1107 72-73 0.03450758577391966 1107 74-75 -0.13123332201439197 1107 76-77 0.0660578084858372 1107 78-79 0.3877984691923402 1107 80-81 0.6285528162560858 1107 82-83 0.18073417307222428 1107 84-85 -0.16010158882309966 1107 86-87 -0.040086734185283746 1107 88-89 -0.028251204591342116 1107 90-91 0.0903938411934071 1107 92-93 0.1545070028008162 1107 94-95 0.24683557816370794 1107 96-97 0.43192335001408466 1107 98-99 0.633770108583338 1107 100-101 0.8777184720011881 1108 1 -0.17771086393792856 1108 2 -0.14549374466677278 1108 3 -0.026016628979164125 1108 4 -0.15095785477802792 1108 5 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-0.7729020571235523 2316 50-51 -0.7772878659251461 2316 52-53 -0.3184066070616822 2316 54-55 -0.5775201942964401 2316 56-57 -0.26104762534234993 2316 58-59 -0.2999032812875484 2316 60-61 -0.6620065392688943 2316 62-63 -0.8014793999001313 2316 64-65 -1.0558922495150682 2316 66-67 -1.3775702887349937 2316 68-69 -1.4671001098905023 2316 70-71 -1.26650299794073 2316 72-73 -1.3786999613958955 2316 74-75 -1.04604813682921 2316 76-77 -0.9577367407804118 2316 78-79 -0.9924950318558814 2316 80-81 -0.6173940740304289 2316 82-83 -0.9662609352436391 2316 84-85 -1.0063978851193944 2316 86-87 -0.6727561961000283 2316 88-89 -0.45546993275346637 2316 90-91 -0.5250848450399985 2316 92-93 -0.15562577148289947 2316 94-95 -0.5096983142192393 2316 96-97 0.02650755686244466 2316 98-99 -0.15738991936914104 2316 100-101 -0.6201850919401117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 4.0 6 19.0 7 33.0 8 68.0 9 169.0 10 251.0 11 479.0 12 742.0 13 1027.0 14 1240.0 15 1795.0 16 2610.0 17 3653.0 18 3052.0 19 1757.0 20 1500.0 21 1486.0 22 1533.0 23 1567.0 24 1858.0 25 1969.0 26 2168.0 27 2507.0 28 3167.0 29 3933.0 30 4864.0 31 6080.0 32 7759.0 33 10306.0 34 14045.0 35 20110.0 36 31138.0 37 52759.0 38 53286.0 39 6632.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.051250086571095 23.626778781323456 17.13496535119346 19.187005780911992 2 14.324341933802451 25.2879039614282 37.524840370080796 22.862913734688558 3 19.407251759186863 28.030117930675008 33.118728824602556 19.443901485535577 4 11.254723742507167 20.27381417774303 41.883714490487364 26.587747589262445 5 10.18245499714914 41.29388287040808 36.577747006597704 11.945915125845076 6 25.723221266614544 41.626351967683085 17.07755082095387 15.572875944748501 7 22.497638128746416 36.36711623664321 24.765441751368257 16.369803883242113 8 23.494103466249676 33.01407349491791 22.227244592129267 21.264578446703155 9 22.90852228303362 19.375081443836333 25.522462210059942 32.19393406307011 10-11 22.27387118842846 28.39009968725567 29.06140050820954 20.274628616106334 12-13 25.00157807393531 27.15732888078924 29.133281070239565 18.707811975035888 14-15 21.556147380766223 29.435431326557204 29.78543621318739 19.222985079489185 16-17 17.52691718790722 32.04855681522022 30.851739640344018 19.57278635652854 18-19 17.107074211623665 31.73907023716445 33.0661975501694 18.08765800104248 20-21 18.944039940057337 28.95246937711754 32.44926048996612 19.654230192859004 22-23 18.694121551596005 28.497983234683883 33.346568037578415 19.461327176141697 24-25 20.52739170979814 29.45978083374395 31.93588715096084 18.076940305497075 26-27 18.812330747459942 28.17583939079266 35.16299146865392 17.848838393093477 28-29 17.6402078480559 30.800117281034677 33.67004121125247 17.889633659656955 30-31 18.88388684938703 28.945316238167667 32.8047596068682 19.366037305577105 32-33 18.3967373599166 29.70562125358353 33.811205043002346 18.086436343497525 34-35 19.889195660672403 29.0984574537399 32.66956606724003 18.342780818347666 36-37 18.847121774824082 28.500659695074276 33.98529124315871 18.666927286942926 38-39 18.134525130564917 30.445396886803017 33.244627240982624 18.175450741649446 40-41 17.7641223612197 30.57931000781861 33.40704000521241 18.249527625749284 42-43 17.825193608230208 30.136090854849318 31.229827172963475 20.808888363957003 44-45 18.972545282772998 28.856772869429243 31.86978759447485 20.30089425332291 46-47 20.700579880114674 30.327648553557466 31.57821866041178 17.393552905916078 48-49 19.607603596559812 29.91065611154548 31.494535118582224 18.987205173312482 50-51 17.295005863956217 30.451850403961426 33.03748859786291 19.215655134219443 52-53 18.815765897836854 31.778570497784724 30.085149530883506 19.32051407349492 54-55 18.909019090435237 29.667546260099037 30.81081411258796 20.612620536877767 56-57 20.571491399530885 29.578161649726347 29.57490389627313 20.275443054469637 58-59 20.943486121970288 29.150785118582224 30.520059616888197 19.38566914255929 60-61 19.698413474068282 30.81020328381548 31.58717748240813 17.904205759708105 62-63 18.379023325514726 30.731202762574927 31.803207258274696 19.086566653635654 64-65 18.502817956737033 31.753526518113105 30.47648716445139 19.267168360698463 66-67 19.298320628094867 30.256588806359137 28.962446247068023 21.482644318477977 68-69 18.563900833984885 29.513821019025283 31.158986512900704 20.763291634089132 70-71 20.78996449048736 29.533774758926246 30.041780688037527 19.634480062548867 72-73 19.300967552775607 28.330238467552775 32.51482277821214 19.853971201459476 74-75 18.194756645817044 31.946955629397966 29.892331248371125 19.965956476413865 76-77 18.319365715402657 29.490202306489444 30.845631352619236 21.344800625488663 78-79 18.704798670836592 29.2633812223091 31.176700547302584 20.85511955955173 80-81 20.087307792546262 29.68688917122752 29.589156567630965 20.636646468595256 82-83 19.0856651864159 28.875681332755075 31.602030407242033 20.436623073586993 84-85 18.468815155069066 29.442557662236123 30.813257427677875 21.27536975501694 86-87 17.92477032838155 30.128925592911127 30.53044370602033 21.415860372686996 88-89 18.417709147771696 29.329757948918427 29.404279059160803 22.848253844149074 90-91 18.870752288795494 27.55127390610237 30.701422148372593 22.876551656729546 92-93 20.14139024236291 29.060133612150114 30.505189127774507 20.293287017712466 94-95 18.759773260359655 28.151876465989055 31.02786193640865 22.06048833724264 96-97 17.05841151941621 28.732367409434456 32.0133323560073 22.195888715142036 98-99 18.144186737849886 29.607413153827828 29.355950701142476 22.892449407179814 100-101 17.1166456237527 28.440109151631166 30.29975970349855 24.143485521117583 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 405.0 1 260.5 2 115.0 3 127.5 4 112.5 5 103.0 6 177.0 7 297.0 8 431.5 9 537.5 10 528.5 11 485.5 12 481.5 13 515.0 14 580.0 15 593.0 16 610.5 17 646.5 18 663.0 19 685.5 20 751.0 21 852.5 22 937.5 23 1080.0 24 1238.5 25 1481.0 26 2016.0 27 2690.5 28 2859.0 29 3152.5 30 4005.0 31 4855.5 32 6188.5 33 7851.5 34 9060.0 35 10384.0 36 12823.0 37 14642.5 38 13979.0 39 12359.0 40 11009.5 41 9363.0 42 7432.0 43 7111.5 44 7647.0 45 7577.0 46 7063.0 47 10058.0 48 16989.0 49 15325.0 50 7501.5 51 3693.5 52 2336.0 53 1785.5 54 1476.5 55 1309.5 56 1128.0 57 989.5 58 906.5 59 802.0 60 652.5 61 520.0 62 414.0 63 288.5 64 193.5 65 141.0 66 99.5 67 74.0 68 55.0 69 48.5 70 35.0 71 24.0 72 29.5 73 32.0 74 29.5 75 24.5 76 13.5 77 7.5 78 9.5 79 6.5 80 2.5 81 3.0 82 1.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042758014073494915 2 0.0 3 0.0 4 0.0 5 0.01140213708626531 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006311897315611155 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.108287724784988E-4 24-25 4.0721918165233256E-4 26-27 0.0012216575449569976 28-29 0.0016288767266093302 30-31 6.108287724784988E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.0360959082616628E-4 40-41 0.0 42-43 0.013641842585353142 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.108287724784988E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009773260359655981 92-93 0.002646924680740162 94-95 0.0 96-97 0.0 98-99 0.0018324863174354966 100-101 0.015474328902788636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 245568.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.03647054990878 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4886166134579 26.590598123534008 2 8.017059671872497 5.297107114933542 3 2.8621790525965465 2.8366888193901483 4 1.6369396131990583 2.1631482929371906 5 1.0304830697548288 1.70217617930675 6 0.7531401383016751 1.492865519937451 7 0.6089218139460353 1.4081639301537658 8 0.46100558383768664 1.218399791503779 9 0.3722558457726774 1.10682173573104 >10 3.106240832275321 21.28331052905916 >50 0.40060645654344423 9.182385327078446 >100 0.23420069767155202 12.234493093562678 >500 0.009861082007223243 2.4571605420901745 >1k 0.018489528763543577 11.026681000781862 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4632 1.8862392494136042 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3166 1.2892559291112848 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2349 0.9565578577013292 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2177 0.8865161584571281 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.8018145686734427 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1650 0.6719116497263488 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1521 0.6193803752931979 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1425 0.5802873338545739 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1244 0.5065806619755018 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1242 0.505766223612197 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 0.500879593432369 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1153 0.4695237164451394 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1145 0.46626596299192075 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1139 0.4638226479020068 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1036 0.42187907219181653 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 940 0.3827860307531926 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 862 0.35102293458431066 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 852 0.3469507427677873 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 829 0.3375847015897837 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 760 0.30948657805577273 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 659 0.2683574407088872 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 579 0.23577990617670053 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 553 0.2251922074537399 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 488 0.19872296064633832 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 455 0.1852847276518113 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.18039809747198332 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 443 0.18039809747198332 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 347 0.14130505603335938 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 336 0.13682564503518374 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 322 0.1311245764920511 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 319 0.12990291894709408 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 313 0.12745960385718008 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 310 0.1262379463122231 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 302 0.12298019285900443 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 295 0.1201296585874381 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 287 0.11687190513421945 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 282 0.11483580922595778 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 279 0.11361415168100078 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 274 0.11157805577273912 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.1038408913213448 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 4.0721918165233256E-4 12-13 0.0 0.0 0.0 0.0 4.0721918165233256E-4 14-15 0.0 0.0 0.0 0.0 4.0721918165233256E-4 16-17 0.0 0.0 0.0 2.0360959082616628E-4 4.0721918165233256E-4 18-19 0.0 0.0 0.0 4.0721918165233256E-4 4.0721918165233256E-4 20-21 0.0 0.0 0.0 8.144383633046651E-4 4.0721918165233256E-4 22-23 0.0 0.0 0.0 0.0024433150899139952 4.0721918165233256E-4 24-25 0.0 0.0 0.0 0.0032577534532186605 4.0721918165233256E-4 26-27 0.0 0.0 0.0 0.004479410998175658 4.0721918165233256E-4 28-29 0.0 0.0 0.0 0.007533554860568152 4.0721918165233256E-4 30-31 0.0 0.0 0.0 0.014659890539483973 4.0721918165233256E-4 32-33 0.0 0.0 0.0 0.02076817826426896 4.0721918165233256E-4 34-35 0.0 0.0 0.0 0.025043979671618453 4.0721918165233256E-4 36-37 0.0 0.0 0.0 0.031559486578055775 4.0721918165233256E-4 38-39 0.0 0.0 0.0 0.03827860307531926 4.0721918165233256E-4 40-41 0.0 0.0 0.0 0.049273520979932234 4.0721918165233256E-4 42-43 0.0 0.0 0.0 0.07024530883502736 4.0721918165233256E-4 44-45 0.0 0.0 0.0 0.10974556945530363 4.0721918165233256E-4 46-47 0.0 0.0 0.0 0.1920038441490748 4.0721918165233256E-4 48-49 0.0 0.0 0.0 0.2475892624446182 4.0721918165233256E-4 50-51 0.0 0.0 0.0 0.31783457127964554 4.0721918165233256E-4 52-53 0.0 0.0 0.0 0.40905166796976805 4.0721918165233256E-4 54-55 0.0 0.0 0.0 0.5159467031535053 4.0721918165233256E-4 56-57 0.0 0.0 0.0 0.6326149986968986 4.0721918165233256E-4 58-59 0.0 0.0 0.0 0.8091445139431848 4.0721918165233256E-4 60-61 0.0 0.0 0.0 1.013975762314308 4.0721918165233256E-4 62-63 0.0 0.0 0.0 1.2554567370341412 4.0721918165233256E-4 64-65 0.0 0.0 0.0 1.4924583007557988 4.0721918165233256E-4 66-67 0.0 0.0 0.0 1.8186408652593173 4.0721918165233256E-4 68-69 0.0 0.0 0.0 2.2236203414125617 4.0721918165233256E-4 70-71 0.0 0.0 0.0 2.547970419598645 4.0721918165233256E-4 72-73 0.0 0.0 0.0 3.017086916862132 4.0721918165233256E-4 74-75 0.0 0.0 0.0 3.4570872426374772 4.0721918165233256E-4 76-77 0.0 0.0 0.0 3.863491985926505 4.0721918165233256E-4 78-79 0.0 0.0 0.0 4.41079456606724 4.0721918165233256E-4 80-81 0.0 0.0 0.0 4.915746351316132 4.0721918165233256E-4 82-83 0.0 0.0 0.0 5.532683411519416 4.0721918165233256E-4 84-85 0.0 0.0 0.0 6.274840370080792 4.0721918165233256E-4 86-87 0.0 0.0 0.0 7.187214946572843 4.0721918165233256E-4 88-89 0.0 0.0 0.0 8.355526778733385 4.0721918165233256E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAGAT 50 0.001610709 38.01263 7 TGCGCCA 30 0.003942349 31.67719 12-13 TATGGTG 75 3.069135E-4 31.670742 2 GATCCTC 60 0.0039424724 31.67074 5 TAGGCTG 65 0.0058373823 29.228577 6 GTACTGG 70 0.008365372 27.15188 1 CTAGATA 70 0.008373692 27.146349 4 ACGCCTG 70 5.79852E-7 27.140821 88-89 ATGGTGG 90 8.9207344E-4 26.392284 3 TGGGAGA 115 1.2783971E-4 24.780748 6 GAGTACT 1560 0.0 23.910187 12-13 GTATATA 100 0.0016467674 23.757893 1 ATGGGAG 140 1.7703338E-5 23.753056 5 GTACTTT 1590 0.0 23.300138 14-15 ACATGGG 910 0.0 22.969988 3 GTACATG 965 0.0 22.650015 1 CATGGGC 210 3.4136974E-8 22.621958 4 CCCTTGG 255 4.820322E-10 22.360373 8 CCTTGGC 255 4.820322E-10 22.360373 9 TGCCGTC 65 2.9336117E-4 21.92813 42-43 >>END_MODULE