##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937509_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267493 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.748135465227126 33.0 31.0 34.0 30.0 34.0 2 31.845995969988 33.0 31.0 34.0 30.0 34.0 3 31.853349433443118 33.0 31.0 34.0 30.0 34.0 4 35.48927635489527 37.0 35.0 37.0 33.0 37.0 5 35.49922801718176 37.0 35.0 37.0 33.0 37.0 6 35.600755907631225 37.0 35.0 37.0 33.0 37.0 7 35.564702627732316 37.0 35.0 37.0 33.0 37.0 8 35.56446710755048 37.0 35.0 37.0 33.0 37.0 9 37.15450123928477 39.0 37.0 39.0 34.0 39.0 10-11 37.0804002347725 39.0 37.0 39.0 33.5 39.0 12-13 37.02560440833966 39.0 37.0 39.0 33.0 39.0 14-15 38.11038606617743 40.0 38.0 41.0 33.0 41.0 16-17 38.08552747174693 40.0 38.0 41.0 33.0 41.0 18-19 38.153418967972996 40.0 38.0 41.0 33.5 41.0 20-21 38.10939912446307 40.0 38.0 41.0 33.5 41.0 22-23 37.982564029712925 40.0 38.0 41.0 33.0 41.0 24-25 37.82250937407708 40.0 37.5 41.0 33.0 41.0 26-27 37.65904154501239 40.0 37.0 41.0 32.5 41.0 28-29 37.53149802050895 40.0 37.0 41.0 33.0 41.0 30-31 37.52115008617048 39.5 37.0 41.0 33.0 41.0 32-33 37.28571962630798 39.0 36.0 41.0 32.5 41.0 34-35 37.05914360375785 39.0 36.0 41.0 32.0 41.0 36-37 36.99019600512911 39.0 36.0 41.0 31.5 41.0 38-39 36.91763709704553 39.0 36.0 41.0 31.0 41.0 40-41 36.82370753627198 39.5 36.0 41.0 31.0 41.0 42-43 36.52392025211874 39.0 35.0 41.0 30.5 41.0 44-45 36.2247479373292 39.0 35.0 41.0 30.0 41.0 46-47 35.7953441772308 39.0 35.0 41.0 29.0 41.0 48-49 35.38453716545854 39.0 35.0 41.0 26.5 41.0 50-51 34.41369867622704 38.0 34.0 40.0 25.0 40.5 52-53 34.56670454927792 38.5 34.5 40.0 24.0 40.5 54-55 34.979939661972466 39.0 35.0 40.0 24.0 41.0 56-57 34.7313967094466 39.0 35.0 40.5 23.0 41.0 58-59 34.492468961804605 39.0 35.0 40.0 22.0 41.0 60-61 34.18136736288426 38.0 34.0 40.0 20.5 41.0 62-63 33.895731850926936 38.0 34.0 40.0 19.0 41.0 64-65 33.56257360005682 38.0 34.0 40.0 16.0 41.0 66-67 33.11599742797007 37.0 33.0 40.0 10.5 41.0 68-69 32.60214472902095 36.5 32.5 39.0 7.5 41.0 70-71 32.10498779407311 36.0 32.0 39.0 4.0 40.0 72-73 31.64868239542717 35.5 31.5 38.5 2.0 40.0 74-75 31.216594452938956 35.0 31.0 37.5 2.0 40.0 76-77 30.827926338259317 35.0 31.0 37.0 2.0 39.0 78-79 30.355487807157573 35.0 31.0 36.5 2.0 39.0 80-81 29.87417614666552 34.5 30.0 36.0 2.0 38.5 82-83 29.414623934084254 34.0 29.5 35.5 2.0 37.0 84-85 28.912928562616592 34.0 29.0 35.0 2.0 37.0 86-87 28.60775609081359 34.0 29.0 35.0 2.0 36.0 88-89 28.185556257546928 34.0 28.5 35.0 2.0 36.0 90-91 27.75534126126665 34.0 27.5 35.0 2.0 36.0 92-93 27.349330636689558 34.0 26.5 35.0 2.0 35.0 94-95 26.84763152680631 33.5 25.5 35.0 2.0 35.0 96-97 26.275113741294163 33.0 25.0 35.0 2.0 35.0 98-99 25.700425805535097 33.0 23.5 35.0 2.0 35.0 100-101 23.916911844422096 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22685935950713443 1101 2 0.23220523669501958 1101 3 0.3435709468516599 1101 4 0.42411148624982786 1101 5 0.43609295675168624 1101 6 0.14963834596126446 1101 7 0.44233103149898056 1101 8 0.14528736198961667 1101 9 0.3517137772756769 1101 10-11 0.42043579987320356 1101 12-13 0.545153808809907 1101 14-15 0.46993024840164566 1101 16-17 0.535621898939624 1101 18-19 0.44216542571906103 1101 20-21 0.5159610605761671 1101 22-23 0.40414272531624107 1101 24-25 0.36855167485664 1101 26-27 0.592425073492322 1101 28-29 0.610812357488669 1101 30-31 0.3729968007486164 1101 32-33 0.3012816777984213 1101 34-35 0.4859855934080386 1101 36-37 0.43917585164017936 1101 38-39 0.4165908133128937 1101 40-41 0.611257286888268 1101 42-43 0.6670381129843292 1101 44-45 0.6877798134020239 1101 46-47 0.1992349870681096 1101 48-49 0.46348429714052486 1101 50-51 0.25271527075305755 1101 52-53 0.5337662171201245 1101 54-55 0.5435606016066004 1101 56-57 0.6053190987822319 1101 58-59 0.5031010509357898 1101 60-61 0.41290367637854075 1101 62-63 0.5681691977389534 1101 64-65 0.5756094410772761 1101 66-67 0.8432217453117232 1101 68-69 0.40389710556384983 1101 70-71 0.366154369266976 1101 72-73 0.4718051173955047 1101 74-75 0.4322053753689339 1101 76-77 0.761224610766547 1101 78-79 0.94429921292204 1101 80-81 1.101463167850003 1101 82-83 1.166835934757227 1101 84-85 0.9671539628308423 1101 86-87 1.5009575204786678 1101 88-89 1.574097421447565 1101 90-91 1.6361601828520271 1101 92-93 1.6144189962988627 1101 94-95 1.2601997877629678 1101 96-97 1.2427253158016072 1101 98-99 1.3833280612578385 1101 100-101 1.3724344743481574 1102 1 -0.16918024445326196 1102 2 -0.1836363474633913 1102 3 -0.03926733697672091 1102 4 -0.05113603850264781 1102 5 0.14896424388039975 1102 6 0.15623900602727048 1102 7 0.0825950579016208 1102 8 0.07598043129654286 1102 9 -0.11693308741079278 1102 10-11 -0.2445807017769539 1102 12-13 -0.04230493706467797 1102 14-15 0.01778503388019459 1102 16-17 -0.06833849709997963 1102 18-19 0.031274336610152886 1102 20-21 0.01926139060916654 1102 22-23 0.22922523356706392 1102 24-25 0.2563404537345306 1102 26-27 0.12707853883885178 1102 28-29 0.3038816644193645 1102 30-31 0.10897039810835452 1102 32-33 0.23362491212184722 1102 34-35 0.12624961981067884 1102 36-37 0.35831776583159325 1102 38-39 0.6113102852600889 1102 40-41 0.6030064618057693 1102 42-43 0.5284242515981958 1102 44-45 0.17952898831951813 1102 46-47 0.3708521487842802 1102 48-49 0.1961575644672564 1102 50-51 0.12070206943293016 1102 52-53 0.5172645669551059 1102 54-55 0.5864648920356359 1102 56-57 0.8115897258449394 1102 58-59 0.8380845492856253 1102 60-61 0.8056429503059306 1102 62-63 0.9840107818973678 1102 64-65 0.9584477249056604 1102 66-67 1.0560930324404367 1102 68-69 1.011157831636453 1102 70-71 1.0592236761976714 1102 72-73 0.5906169985836272 1102 74-75 0.9553076855999585 1102 76-77 0.9691454028457542 1102 78-79 0.9426490479055367 1102 80-81 0.77803082461568 1102 82-83 1.1618854397077207 1102 84-85 1.2097282202565829 1102 86-87 0.9415515798846066 1102 88-89 0.9470347151769403 1102 90-91 1.0404506118949293 1102 92-93 0.7530328576850032 1102 94-95 0.515975365320724 1102 96-97 0.7592269659666258 1102 98-99 1.302469975449256 1102 100-101 0.8707843093316541 1103 1 0.21236110456981905 1103 2 0.13393342868355163 1103 3 0.22967265606411402 1103 4 0.17200001312168212 1103 5 0.17429137723271282 1103 6 0.08799703806050019 1103 7 0.09440454555965516 1103 8 0.16264309117275388 1103 9 0.2920125994901639 1103 10-11 0.2790013688901709 1103 12-13 0.4125290447360541 1103 14-15 0.5103039343325335 1103 16-17 0.5032143450851905 1103 18-19 0.45810296196470546 1103 20-21 0.38658390560827627 1103 22-23 0.38136201274019044 1103 24-25 0.44202883087896083 1103 26-27 0.06794674706542736 1103 28-29 -0.0786033891978164 1103 30-31 0.27694454376572963 1103 32-33 0.4515626803395705 1103 34-35 0.2559905491751735 1103 36-37 0.2310230874819723 1103 38-39 0.17538729999031233 1103 40-41 0.4344734743217131 1103 42-43 0.26568951167053 1103 44-45 -0.00732979340529738 1103 46-47 0.07191922034767373 1103 48-49 0.4135364908582986 1103 50-51 0.33759069120244334 1103 52-53 0.35875382770226594 1103 54-55 0.6269861965622567 1103 56-57 0.9583858751084335 1103 58-59 1.0406067184354413 1103 60-61 0.76299699241941 1103 62-63 1.2374560944797466 1103 64-65 0.955859127387761 1103 66-67 1.1568058193740853 1103 68-69 1.2747255622114295 1103 70-71 1.1409622014878984 1103 72-73 1.4169173602491867 1103 74-75 1.5776307805292582 1103 76-77 1.1955079752052278 1103 78-79 1.623545092781594 1103 80-81 1.382623871779817 1103 82-83 1.4236448424850607 1103 84-85 1.2646423378664267 1103 86-87 1.385403792581915 1103 88-89 1.5967094270570712 1103 90-91 1.551279490309927 1103 92-93 1.4738017396850225 1103 94-95 1.1444351761580371 1103 96-97 1.1618092369732764 1103 98-99 1.3553016164408334 1103 100-101 1.941546337611161 1104 1 0.14691013381533224 1104 2 0.10398995362825403 1104 3 -0.021445554798503963 1104 4 0.04379160810816529 1104 5 -0.005988789884519008 1104 6 -0.10281151440938885 1104 7 0.13223202159799285 1104 8 -0.05301677611650035 1104 9 0.47075922028151496 1104 10-11 0.2804611870273064 1104 12-13 0.037075616375283005 1104 14-15 0.19670354111553223 1104 16-17 0.2866500786806725 1104 18-19 -0.06283584363864492 1104 20-21 -0.03200542838042253 1104 22-23 0.4011064216858742 1104 24-25 -0.06930564933731631 1104 26-27 0.13933037940752513 1104 28-29 0.08363794774000866 1104 30-31 -0.17113115050803884 1104 32-33 -0.03569217343285658 1104 34-35 0.057140708919583005 1104 36-37 0.0024558719498983805 1104 38-39 0.15538238477773092 1104 40-41 -0.12207604644506631 1104 42-43 0.12713458416990875 1104 44-45 0.41315681264041615 1104 46-47 1.195020719487509 1104 48-49 1.4231948835837827 1104 50-51 0.5608543923575198 1104 52-53 0.6620322744950897 1104 54-55 0.7793971083341944 1104 56-57 1.035539967022899 1104 58-59 1.049336135982756 1104 60-61 0.9192860069192861 1104 62-63 1.3068490657257499 1104 64-65 1.1474246225954303 1104 66-67 1.4768648019250747 1104 68-69 1.5246739524793114 1104 70-71 1.3761010562433675 1104 72-73 1.2607413956387177 1104 74-75 1.520943633810166 1104 76-77 1.6758323792356968 1104 78-79 1.5185414063721545 1104 80-81 1.7279470470071487 1104 82-83 1.7272624389461093 1104 84-85 2.1239247168293147 1104 86-87 1.848741221925735 1104 88-89 1.6318531970251229 1104 90-91 1.5870868139766756 1104 92-93 1.6146373258241233 1104 94-95 1.7183059060671049 1104 96-97 1.4069751253979526 1104 98-99 1.2107157231009467 1104 100-101 1.1164050252493993 1105 1 0.22633500800656492 1105 2 0.13449079235339312 1105 3 0.2938192427092865 1105 4 0.17165166082803296 1105 5 0.18656333089190014 1105 6 0.04643363296659686 1105 7 0.06593918670702692 1105 8 0.07107620255591485 1105 9 0.11048124280727478 1105 10-11 0.030765524434443137 1105 12-13 -0.08388292663934038 1105 14-15 -0.25173163741310844 1105 16-17 0.11145237918390904 1105 18-19 0.0016022105190458547 1105 20-21 0.17985174871039789 1105 22-23 0.10808461483229337 1105 24-25 -0.060429541021953526 1105 26-27 0.08964909495988138 1105 28-29 0.32166334912988503 1105 30-31 0.30160788615622636 1105 32-33 0.2519617925388644 1105 34-35 0.1477326092311131 1105 36-37 -0.1703981898761242 1105 38-39 -0.3609607736215068 1105 40-41 -0.04762121778463069 1105 42-43 -0.1959519243371588 1105 44-45 -0.4758486989819204 1105 46-47 -0.5469634645527535 1105 48-49 -0.37122160335606935 1105 50-51 -0.30766226232734795 1105 52-53 -0.14318699221950482 1105 54-55 -0.05207185883570986 1105 56-57 -0.11518612931067196 1105 58-59 -0.19960707034363168 1105 60-61 -0.1346272449378887 1105 62-63 -0.06773037352658662 1105 64-65 0.05764269113125664 1105 66-67 0.15528799866603293 1105 68-69 0.09712063352412059 1105 70-71 0.3173279913020757 1105 72-73 0.3405784433262298 1105 74-75 0.0668866005007942 1105 76-77 -0.06595530348656098 1105 78-79 0.12120615595279105 1105 80-81 0.05132591113367724 1105 82-83 0.26305443511193616 1105 84-85 0.06685785845404979 1105 86-87 0.11247474696767412 1105 88-89 0.12562266743010753 1105 90-91 0.33609232559900803 1105 92-93 0.04238235338223362 1105 94-95 0.637702023967897 1105 96-97 0.42282463414466065 1105 98-99 0.8319570363048783 1105 100-101 0.6442027726733137 1106 1 -0.019929650604069593 1106 2 0.16585178606333884 1106 3 -0.07622262486220066 1106 4 0.10945549835804513 1106 5 0.3155888681262624 1106 6 -0.07336516458087772 1106 7 0.041403996120287445 1106 8 0.09361722682323403 1106 9 0.19198411004143878 1106 10-11 0.24927059418065767 1106 12-13 -0.13975926848507925 1106 14-15 0.02916801931250035 1106 16-17 -0.5605816704619073 1106 18-19 -0.008671218454907148 1106 20-21 -0.10360758418639904 1106 22-23 0.260851644615812 1106 24-25 0.3155204163167795 1106 26-27 0.29345090855990463 1106 28-29 0.3435644543544072 1106 30-31 0.3286630680122826 1106 32-33 0.3060324713491056 1106 34-35 0.43522988089410575 1106 36-37 0.6128478366730761 1106 38-39 0.4366660692461295 1106 40-41 0.7030831892237259 1106 42-43 0.7538714778494793 1106 44-45 0.4716134515429147 1106 46-47 0.4327228262978977 1106 48-49 0.23483133630303854 1106 50-51 0.34211847858976085 1106 52-53 0.5332669633730944 1106 54-55 0.8248454179863245 1106 56-57 0.4250990067093312 1106 58-59 0.43467175577697503 1106 60-61 0.6988499461647493 1106 62-63 0.7617535243244831 1106 64-65 0.34525901749989174 1106 66-67 0.8415242825718181 1106 68-69 0.374840537486655 1106 70-71 0.7287860145971159 1106 72-73 0.8952195926009949 1106 74-75 0.8117275632565146 1106 76-77 0.946332134257748 1106 78-79 0.8314468448826879 1106 80-81 0.419309124420181 1106 82-83 0.6017700332626212 1106 84-85 0.7632534772408803 1106 86-87 0.6092850729791408 1106 88-89 0.7051016046684353 1106 90-91 0.8806683916346891 1106 92-93 0.9290224311646433 1106 94-95 0.9479139413439306 1106 96-97 0.6294788000672327 1106 98-99 1.1554633958104006 1106 100-101 1.5607950301489595 1107 1 -0.02323231632711753 1107 2 0.1346927076643425 1107 3 -0.09613691282319436 1107 4 -0.12129861031538525 1107 5 0.09725907336334672 1107 6 -0.05504323330777794 1107 7 -0.18048402778472195 1107 8 -0.05777461087433977 1107 9 -0.06446395160832452 1107 10-11 -0.22775818619206234 1107 12-13 -0.11093957830657786 1107 14-15 0.19597229705095742 1107 16-17 -0.1599073762101213 1107 18-19 -0.09664401078014606 1107 20-21 0.2781845051428462 1107 22-23 0.08585950810562792 1107 24-25 -0.1596997613980946 1107 26-27 -0.11757370416322743 1107 28-29 -0.07654248910710493 1107 30-31 0.007301897925003686 1107 32-33 -0.06899701673770409 1107 34-35 0.09423947435168856 1107 36-37 0.057636308797007985 1107 38-39 0.24315541421742637 1107 40-41 0.29798568195000286 1107 42-43 0.0424812128498786 1107 44-45 0.24350344132037804 1107 46-47 0.061782352915805916 1107 48-49 0.47604388643278384 1107 50-51 0.5262912394610382 1107 52-53 0.4706932431560631 1107 54-55 0.8967570323607816 1107 56-57 0.853531420014356 1107 58-59 1.014682764662723 1107 60-61 0.8399602709268876 1107 62-63 0.5273428928862458 1107 64-65 0.7011900546941945 1107 66-67 1.0364896832166295 1107 68-69 1.1321240949294449 1107 70-71 1.0725777893867665 1107 72-73 1.4124602940242852 1107 74-75 1.1766131494796817 1107 76-77 0.9666395967095873 1107 78-79 0.9942014253655067 1107 80-81 1.145620361347131 1107 82-83 1.185201660218663 1107 84-85 1.064651212555816 1107 86-87 0.9727060842239297 1107 88-89 1.014569246407838 1107 90-91 0.9886537655436278 1107 92-93 0.6924198519399809 1107 94-95 0.5571378148990149 1107 96-97 0.8449285916847522 1107 98-99 1.1076435483621054 1107 100-101 1.4442777697888118 1108 1 -0.11177567017659129 1108 2 -0.1381629954634711 1108 3 -0.09841348482765966 1108 4 -0.04423709787972285 1108 5 -0.006401438460429176 1108 6 -0.18323313360652094 1108 7 -0.11336564604049215 1108 8 -0.3132275857150688 1108 9 -0.5163499970317815 1108 10-11 -0.375703872403065 1108 12-13 -0.12845028629347865 1108 14-15 -0.09552790091182572 1108 16-17 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-0.8369011357143386 2316 94-95 -0.26849571336608946 2316 96-97 -0.9741584778409162 2316 98-99 -1.3228470299354882 2316 100-101 -2.1669957844672005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1082.0 3 156.0 4 122.0 5 139.0 6 158.0 7 203.0 8 261.0 9 290.0 10 410.0 11 609.0 12 958.0 13 1349.0 14 1610.0 15 2156.0 16 2804.0 17 4021.0 18 3193.0 19 1936.0 20 1844.0 21 1821.0 22 1963.0 23 2084.0 24 2238.0 25 2548.0 26 2893.0 27 3128.0 28 3789.0 29 4584.0 30 5628.0 31 7171.0 32 9186.0 33 12011.0 34 15860.0 35 23347.0 36 33977.0 37 52457.0 38 51493.0 39 8014.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.49115884078412 23.304725537709025 16.624155082259918 19.579960539246947 2 13.995178084657237 24.783744970922275 38.34847524737626 22.872601697044235 3 18.93996775282163 28.32239679028085 32.947242116314825 19.7903933405827 4 10.826336886865679 19.84340959116254 42.437650693895 26.89260282807678 5 9.90747128160523 41.14716115293483 36.47761014966558 12.467757415794367 6 25.025288665432832 41.719179673158045 17.62189136738624 15.633640294022882 7 22.2017812381741 36.56071908967264 24.81893492897903 16.41856474317423 8 22.912935640262862 32.97003439413126 22.4127595483054 21.70427041730047 9 23.076231402977022 19.31018204570063 25.47515838612871 32.13842816519364 10-11 21.79094243760608 28.424312364880304 29.364576815427906 20.420168382085706 12-13 24.75828464056022 27.38591465916308 29.08920940671314 18.76659129356356 14-15 21.451408984912764 29.562179829367196 29.731314209722886 19.25509697599715 16-17 17.53592590580491 32.176797981292864 30.564979477601455 19.722296635300772 18-19 17.063444493560397 31.768136898652145 32.823609623064186 18.344808984723272 20-21 18.869825998834873 29.29303714360107 32.10807590938977 19.72906094817429 22-23 18.52212596581597 28.63366892999298 33.26939826738469 19.574806836806367 24-25 20.126646052190463 29.820818872921308 31.820335662606823 18.232199412281407 26-27 18.513974224486738 28.39277686198112 34.750674359358605 18.342574554173535 28-29 17.566395471039268 30.878460273243803 33.29430564115629 18.26083861456064 30-31 18.610598495564446 29.091437082556602 32.47332577680771 19.82463864507124 32-33 18.200485241356056 29.872694400989218 33.6002473044749 18.32657305317983 34-35 19.674879597177394 29.316284306140684 32.35955813896855 18.649277957713366 36-37 18.665018309872348 28.644882133123545 33.52139585850355 19.168703698500558 38-39 17.885857585116124 30.594609577355058 33.044290912768496 18.475241924760322 40-41 17.882125553197824 30.568920536164107 33.08511614579139 18.463837764846676 42-43 17.846922033847516 30.283710790299743 30.81805650242357 21.051310673429178 44-45 18.751159092058604 28.847389490524012 31.709386819226598 20.692064598190786 46-47 20.53881829352354 30.332926244493063 31.365570773518748 17.76268468846465 48-49 19.32703998381389 29.969220162947668 31.382132707370484 19.321607145867954 50-51 17.05470071699025 30.34571782396175 32.945955009657915 19.653626449390078 52-53 18.59109961083516 31.87179482375161 30.07174348472102 19.465362080692213 54-55 18.77301096282084 29.503497197932223 30.75922827437218 20.964263564874756 56-57 20.106633606627987 29.620695983729668 29.681934461719393 20.590735947922948 58-59 20.841751661351964 28.80500624469856 30.670738672004465 19.682503421945015 60-61 19.61476448987006 30.478023860931703 31.749720511375234 18.157491137823005 62-63 18.1615592626162 30.692926538775573 31.81353418377666 19.331980014831572 64-65 18.48758536873112 31.366255498523426 30.73049604218671 19.415663090558738 66-67 19.235941251605993 30.0469342882507 29.187283916859265 21.529840543284053 68-69 18.45021628808449 29.368925675549146 31.34997471957454 20.83088331679182 70-71 20.70774981553409 29.18119608819905 30.27506957267582 19.835984523591037 72-73 19.133697071179864 28.16627052580909 32.62215279611763 20.07787960689341 74-75 17.999168473924914 31.819169297959764 30.168477670528393 20.01318455758693 76-77 18.31865750340438 29.21979279638714 30.988384834538678 21.473164865669798 78-79 18.558309499326654 29.13742435395326 31.524009132779295 20.780257013940787 80-81 20.118731038615678 29.556350425109557 29.792688864751486 20.53222967152328 82-83 19.08439981045956 28.730921874502503 31.783796539950515 20.400881775087417 84-85 18.49830156810019 29.16715041702402 31.168191572631837 21.166356442243952 86-87 18.549432192724876 30.17262689518787 30.664401929645834 20.61353898244142 88-89 18.771047239463737 29.256927093673564 29.710180214939374 22.261845451923328 90-91 19.36516973880863 27.44176290131064 30.904524495353392 22.28854286452734 92-93 20.823500325893963 28.864840162122878 30.811401044359037 19.50025846762412 94-95 19.38096870194838 27.775686371459983 31.494893534932416 21.34845139165922 96-97 18.02397228204888 28.35808596310516 32.36613915160596 21.25180260324 98-99 19.13672972466754 29.369357557595055 29.902603483798462 21.59130923393894 100-101 18.730314481425857 27.746984206643173 30.970259652224065 22.552441659706904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 695.0 1 417.5 2 161.5 3 169.0 4 144.0 5 137.5 6 196.5 7 357.0 8 541.0 9 659.0 10 616.0 11 509.5 12 513.5 13 557.0 14 591.5 15 594.0 16 612.5 17 648.5 18 692.0 19 728.5 20 786.0 21 917.0 22 1027.5 23 1177.5 24 1364.5 25 1637.0 26 2192.0 27 2813.0 28 2981.0 29 3374.0 30 4392.0 31 5374.5 32 6850.5 33 8664.0 34 9947.5 35 11265.0 36 13792.0 37 16052.0 38 15394.5 39 13637.0 40 12224.5 41 10270.0 42 8183.5 43 7706.0 44 8148.5 45 8048.0 46 7680.0 47 10885.5 48 18091.0 49 16621.0 50 8284.5 51 3917.5 52 2405.5 53 1889.5 54 1617.0 55 1426.5 56 1247.0 57 1050.0 58 937.5 59 856.5 60 715.0 61 562.0 62 468.5 63 361.0 64 228.0 65 187.0 66 155.0 67 105.0 68 77.5 69 56.5 70 49.0 71 39.0 72 26.5 73 24.0 74 23.5 75 25.5 76 21.0 77 11.0 78 6.5 79 7.0 80 7.5 81 4.5 82 2.0 83 1.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33608356106514936 2 0.296830197425727 3 0.3001947714519632 4 0.30281166236125806 5 0.28598879223007706 6 0.21495889612064617 7 0.22430493508241336 8 0.2194449948222944 9 0.217575787029941 10-11 0.22430493508241336 12-13 0.22374417274470731 14-15 0.2039305701457608 16-17 0.22037959871847113 18-19 0.20598669871734962 20-21 0.21290276754905735 22-23 0.2076689857304677 24-25 0.19757526365175912 26-27 0.21421121300370477 28-29 0.22000575716000043 30-31 0.23009947923870905 32-33 0.23009947923870905 34-35 0.21551965845835216 36-37 0.20953819352282116 38-39 0.19421068962552293 40-41 0.195332214300935 42-43 0.19850986754793584 44-45 0.21738886625070564 46-47 0.20822974806817374 48-49 0.22337033118623664 50-51 0.22935179612176768 52-53 0.23907167664200557 54-55 0.23795015196659353 56-57 0.18841614546922725 58-59 0.17439708702657641 60-61 0.18224775975446086 62-63 0.18505157144299103 64-65 0.18299544287140224 66-67 0.195892976638641 68-69 0.18243468053369621 70-71 0.1891638285861686 72-73 0.20280904547034878 74-75 0.19215456105393414 76-77 0.2084166688474091 78-79 0.20280904547034878 80-81 0.18804230391075655 82-83 0.1986967883271712 84-85 0.18019163118287207 86-87 0.18729462079381518 88-89 0.1996313922233479 90-91 0.195892976638641 92-93 0.2000052337818186 94-95 0.19720142209328842 96-97 0.19364992728781688 98-99 0.20299596624958413 100-101 0.18019163118287207 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267493.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.53396163637927 #Duplication Level Percentage of deduplicated Percentage of total 1 81.45617105717885 27.31548115277783 2 7.571821941784372 5.078263730265839 3 2.816022117925107 2.832971330090881 4 1.5495925352002764 2.0785590650970307 5 0.9732332972876557 1.6318184027245572 6 0.6867259004916334 1.3817184001076666 7 0.5250777583304533 1.2325556182778614 8 0.43589257644842305 1.169376394896315 9 0.33890369115171515 1.022830503975805 >10 2.9776702600862865 20.770636988631477 >50 0.42362961393964393 9.925493377396792 >100 0.21961851038449962 12.315462460699907 >500 0.00891851818820303 2.4654850781141935 >1k 0.016722221602880682 10.779347496943844 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4778 1.7862149663729516 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3496 1.3069500884135286 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2440 0.912173402668481 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2379 0.889369067601769 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.7431970182397296 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1689 0.6314183922569936 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1593 0.5955296026438075 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1452 0.5428179428994403 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1450 0.5420702597824989 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1363 0.509546044195549 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1336 0.4994523221168405 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1308 0.48898475847966116 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1294 0.48375097666107153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1243 0.46468505717906633 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1025 0.3831875974324562 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 995 0.3719723506783355 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 917 0.34281270911762174 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 908 0.33944813509138555 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 877 0.32785904677879424 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 812 0.3035593454781994 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 755 0.28225037664537017 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 755 0.28225037664537017 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 576 0.21533273767911681 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 477 0.17832242339051863 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.1749578493642824 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 450 0.16822870131181003 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 442 0.1652379688440445 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 397 0.1484150987128635 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 386 0.14430284156968592 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.13047070390627044 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 335 0.1252369220876808 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 334 0.12486308052921012 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 330 0.12336771429532736 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 317 0.1185077740352084 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 302 0.11290015065814807 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 298 0.11140478442426532 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 291 0.1087878935149705 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 287 0.10729252728108774 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 286 0.10691868572261705 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 281 0.1050494779302636 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 278 0.10392795325485152 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 268 0.10018953767014463 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8692077923534447E-4 0.0 12-13 0.0 0.0 0.0 3.7384155847068894E-4 0.0 14-15 0.0 0.0 0.0 3.7384155847068894E-4 0.0 16-17 0.0 0.0 0.0 7.476831169413779E-4 0.0 18-19 0.0 0.0 0.0 7.476831169413779E-4 0.0 20-21 0.0 0.0 0.0 7.476831169413779E-4 0.0 22-23 0.0 0.0 0.0 7.476831169413779E-4 0.0 24-25 0.0 0.0 0.0 0.0022430493508241336 0.0 26-27 0.0 0.0 0.0 0.002429970130059478 0.0 28-29 0.0 0.0 0.0 0.005046861039354301 0.0 30-31 0.0 0.0 0.0 0.013084454546474113 0.0 32-33 0.0 0.0 0.0 0.019813602598946517 0.0 34-35 0.0 0.0 0.0 0.02766427532683098 0.0 36-37 0.0 0.0 0.0 0.0370103142885982 0.0 38-39 0.0 0.0 0.0 0.04392638312030595 0.0 40-41 0.0 0.0 0.0 0.057384679225250756 0.0 42-43 0.0 0.0 0.0 0.07906748961655072 0.0 44-45 0.0 0.0 0.0 0.12448923897073942 0.0 46-47 0.0 0.0 0.0 0.21794962858841166 0.0 48-49 0.0 0.0 0.0 0.27626891170983914 0.0 50-51 0.0 0.0 0.0 0.3508503026247416 0.0 52-53 0.0 0.0 0.0 0.44094611821617763 0.0 54-55 0.0 0.0 0.0 0.5564631597836205 0.0 56-57 0.0 0.0 0.0 0.6845038935598315 0.0 58-59 0.0 0.0 0.0 0.8678731779897044 0.0 60-61 0.0 0.0 0.0 1.075542163720172 0.0 62-63 0.0 0.0 0.0 1.3198476221807673 0.0 64-65 0.0 0.0 0.0 1.5424702702500626 0.0 66-67 0.0 0.0 0.0 1.8721985248212103 0.0 68-69 0.0 0.0 0.0 2.286601892385969 0.0 70-71 0.0 0.0 0.0 2.602311088514466 0.0 72-73 0.0 0.0 0.0 3.065313858680414 0.0 74-75 0.0 0.0 0.0 3.5309335197556573 0.0 76-77 0.0 0.0 0.0 3.930196304202353 0.0 78-79 0.0 0.0 0.0 4.484977176972855 0.0 80-81 0.0 0.0 0.0 4.958260589996748 0.0 82-83 0.0 0.0 0.0 5.541640341990258 0.0 84-85 0.0 0.0 0.0 6.281472786203751 0.0 86-87 0.0 0.0 0.0 7.152710538219692 0.0 88-89 0.0 0.0 0.0 8.256477739604401 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 50 0.0016108521 38.013134 2 CCTGTAC 60 0.0039388873 31.677612 3 GGCTTCG 60 0.0039425073 31.67167 9 CCCGAGT 30 0.0039532697 31.659792 82-83 CGAGTGC 30 0.0039550844 31.656822 84-85 GCAGATC 80 4.4754197E-4 29.69776 8 TCCCGAG 35 0.008396497 27.136965 82-83 CCGAGTG 35 0.008400339 27.134418 84-85 CCCTATA 90 8.911456E-4 26.398008 2 TTAGGCA 95 0.0012202836 25.013334 4 GAATATT 100 0.0016362425 23.784983 1 ACGCAGC 100 0.0016470096 23.75821 7 CTCCCGA 50 0.001654577 23.744844 80-81 GTACATG 1185 0.0 23.28319 1 GAGGGTG 145 2.3194467E-5 22.93896 7 AGCAGAT 105 0.0021874316 22.626865 7 CATGGGC 190 3.2506068E-7 22.512001 4 GAGTACT 1615 0.0 22.205225 12-13 GTACTTT 1615 0.0 21.75998 14-15 ACATGGG 1185 0.0 21.65305 3 >>END_MODULE