##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267493 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02014632158598 31.0 31.0 34.0 30.0 34.0 2 32.256268388331655 33.0 31.0 34.0 30.0 34.0 3 32.306217358958925 34.0 31.0 34.0 30.0 34.0 4 35.94427143887877 37.0 35.0 37.0 35.0 37.0 5 35.83092267835046 37.0 35.0 37.0 35.0 37.0 6 35.9368581607743 37.0 35.0 37.0 35.0 37.0 7 35.89436732923852 37.0 35.0 37.0 35.0 37.0 8 35.88966814084854 37.0 35.0 37.0 35.0 37.0 9 37.543038509418935 39.0 37.0 39.0 35.0 39.0 10-11 37.460288680451455 39.0 37.0 39.0 34.5 39.0 12-13 37.43363564654028 39.0 37.0 39.0 34.0 39.0 14-15 38.513432127195856 40.0 38.0 41.0 34.0 41.0 16-17 38.528054939755435 40.0 38.0 41.0 34.0 41.0 18-19 38.62577712313967 40.0 38.0 41.0 34.0 41.0 20-21 38.54198427622405 40.0 38.0 41.0 34.0 41.0 22-23 38.42512140504611 40.0 38.0 41.0 34.0 41.0 24-25 38.244249382226826 40.0 37.5 41.0 33.5 41.0 26-27 38.16026961453197 40.0 37.0 41.0 34.0 41.0 28-29 38.0920846526825 40.0 37.0 41.0 33.5 41.0 30-31 38.14598139016722 40.0 37.0 41.0 34.0 41.0 32-33 37.96510002130897 40.0 37.0 41.0 33.5 41.0 34-35 37.79234783713966 40.0 37.0 41.0 33.0 41.0 36-37 37.60415038898214 40.0 36.5 41.0 33.0 41.0 38-39 37.43816660622895 40.0 36.0 41.0 33.0 41.0 40-41 37.32806839805154 40.0 36.0 41.0 33.0 41.0 42-43 37.05673606412131 40.0 36.0 41.0 32.5 41.0 44-45 36.71272519280878 40.0 36.0 41.0 31.0 41.0 46-47 36.25990399748778 39.5 36.0 41.0 30.0 41.0 48-49 35.86587312565189 39.0 35.0 41.0 29.0 41.0 50-51 35.60763833072268 39.0 35.0 41.0 28.0 41.0 52-53 35.45749608400968 39.0 35.0 41.0 27.0 41.0 54-55 35.26561068887784 39.0 35.0 40.5 26.5 41.0 56-57 34.972791811374506 39.0 35.0 40.0 25.5 41.0 58-59 34.745748486876295 39.0 35.0 40.0 25.0 41.0 60-61 34.35967857102803 38.0 34.0 40.0 23.0 41.0 62-63 34.019959400806755 38.0 34.0 40.0 22.0 41.0 64-65 33.68124212596217 37.5 34.0 40.0 21.0 41.0 66-67 33.199259419872675 37.0 33.0 39.5 18.5 41.0 68-69 32.761238611851525 36.5 33.0 39.0 16.0 40.0 70-71 32.27607077568385 36.0 32.0 39.0 12.5 40.0 72-73 31.798867633919393 35.0 31.5 38.0 8.0 40.0 74-75 31.3494670888584 35.0 31.0 37.0 6.5 39.5 76-77 29.958241897918825 34.0 29.5 36.0 2.0 38.5 78-79 30.58202270713626 34.5 31.0 36.0 2.0 39.0 80-81 30.37377987461354 34.0 31.0 36.0 2.0 38.0 82-83 30.02212020501471 34.0 30.5 35.5 2.0 37.0 84-85 29.61521422990508 34.0 30.0 35.0 2.0 37.0 86-87 29.35809161361232 34.0 30.0 35.0 2.0 36.0 88-89 28.911614509538566 34.0 29.5 35.0 2.0 36.0 90-91 28.47441802215385 34.0 29.0 35.0 2.0 35.5 92-93 28.15181892610274 34.0 29.0 35.0 2.0 35.0 94-95 27.724959157809735 34.0 28.5 35.0 2.0 35.0 96-97 27.277895122489184 33.0 27.0 35.0 2.0 35.0 98-99 26.880389393367302 33.0 26.5 35.0 2.0 35.0 100-101 25.032621414392153 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33553439097493865 1101 2 0.048000636164857724 1101 3 0.2195724564842294 1101 4 0.14824051115775916 1101 5 0.04677710060409623 1101 6 0.09164863833189685 1101 7 7.367492659398067E-4 1101 8 0.08280511161656534 1101 9 0.13059549618520094 1101 10-11 0.40497281300366694 1101 12-13 0.124710634533713 1101 14-15 0.28524210227106295 1101 16-17 0.2091429895423076 1101 18-19 0.24308811150016396 1101 20-21 0.26859395496322236 1101 22-23 -0.009580592090237872 1101 24-25 0.29303094753458936 1101 26-27 0.3510364797874246 1101 28-29 0.20905210216986347 1101 30-31 -0.18498777486230011 1101 32-33 0.2514384538805956 1101 34-35 -0.23979463013406388 1101 36-37 -0.056806650348320886 1101 38-39 -0.366457728856993 1101 40-41 -0.04366911923230532 1101 42-43 -0.07549364943425729 1101 44-45 0.0383457847491826 1101 46-47 0.24977975330879332 1101 48-49 0.13590000146130166 1101 50-51 -0.06764560338842074 1101 52-53 -0.09423750459345825 1101 54-55 0.04240877378814645 1101 56-57 0.06684772614478618 1101 58-59 0.28081960678934337 1101 60-61 0.30320241354855426 1101 62-63 0.38204988084171276 1101 64-65 0.28588447440685627 1101 66-67 0.2673370680475742 1101 68-69 0.5302156748785691 1101 70-71 0.35647222850151294 1101 72-73 -0.17256898057503278 1101 74-75 -0.05837355401434152 1101 76-77 -0.08200458274548339 1101 78-79 0.12445743388093788 1101 80-81 0.017634134981992844 1101 82-83 0.30927763514704054 1101 84-85 0.3619113140722092 1101 86-87 0.40433367202823334 1101 88-89 0.20682183597703485 1101 90-91 0.1907716316521828 1101 92-93 -0.13975403782108842 1101 94-95 0.11671050831212781 1101 96-97 -0.12915734043098226 1101 98-99 0.03471286649338623 1101 100-101 0.6864331534191983 1102 1 0.12431326886272487 1102 2 0.1041062467259124 1102 3 0.2888793871772961 1102 4 0.0030259897056126306 1102 5 0.12268469136316895 1102 6 0.13125259872793293 1102 7 0.03374004959596988 1102 8 0.04320115122052215 1102 9 0.28571100773636005 1102 10-11 0.16899921564392884 1102 12-13 0.06035419889015259 1102 14-15 -0.15700212215138265 1102 16-17 -0.011979122668911657 1102 18-19 0.15067887057606555 1102 20-21 0.17618471403912395 1102 22-23 0.35840620658962763 1102 24-25 0.2534269871385533 1102 26-27 0.03255463160260774 1102 28-29 -0.030221825222874088 1102 30-31 0.2655072746426512 1102 32-33 0.4692602360588154 1102 34-35 0.3509644457735277 1102 36-37 0.4085398843051493 1102 38-39 0.41242015893178774 1102 40-41 0.7088061282924443 1102 42-43 1.1753314330739997 1102 44-45 1.0119431444851585 1102 46-47 1.0468094562790995 1102 48-49 1.3504214536065149 1102 50-51 1.0379649576676826 1102 52-53 1.3859605152085237 1102 54-55 1.3328378166924395 1102 56-57 1.0866497063428042 1102 58-59 1.1323047553041903 1102 60-61 1.3477568689940966 1102 62-63 1.550366712524884 1102 64-65 1.3106369496543806 1102 66-67 1.0973700713479033 1102 68-69 1.4444070940204838 1102 70-71 1.5231388951681772 1102 72-73 1.626110887411766 1102 74-75 1.322814564797536 1102 76-77 1.1968733050432974 1102 78-79 0.873632351372688 1102 80-81 1.5869410656750595 1102 82-83 1.4033370410876351 1102 84-85 1.2430994328840903 1102 86-87 1.0330465433153648 1102 88-89 1.2183729910925472 1102 90-91 1.2864812026092771 1102 92-93 1.2826882064033356 1102 94-95 1.37908674593589 1102 96-97 1.3559911744204989 1102 98-99 1.2343828334900877 1102 100-101 1.3250470148053388 1103 1 0.33127454006971746 1103 2 0.1858974929431838 1103 3 0.3246347198990662 1103 4 0.21568112103344106 1103 5 0.07943244281563011 1103 6 -0.05605431087676038 1103 7 0.06140799210907488 1103 8 0.02231311992538565 1103 9 0.30397353686467454 1103 10-11 0.049607404258317445 1103 12-13 0.13873440112762125 1103 14-15 0.03474916160319452 1103 16-17 0.10229875879335282 1103 18-19 0.197943181671711 1103 20-21 0.08914246348819432 1103 22-23 0.4012999528332628 1103 24-25 0.368548403433536 1103 26-27 0.24170909337465218 1103 28-29 0.1685097092724277 1103 30-31 0.2706370208441129 1103 32-33 0.24664084951952248 1103 34-35 0.4826033285116367 1103 36-37 0.3882845041345391 1103 38-39 0.6496221538916629 1103 40-41 0.6743319140978628 1103 42-43 0.6982017712260244 1103 44-45 0.3697864368016113 1103 46-47 0.4275599913965067 1103 48-49 0.9819917991394718 1103 50-51 0.8799972439634018 1103 52-53 1.175234969810532 1103 54-55 1.218950070090301 1103 56-57 1.1152349929864869 1103 58-59 1.3099162864253628 1103 60-61 1.0471948639117983 1103 62-63 1.1285096705906525 1103 64-65 1.2211379039031627 1103 66-67 1.2202520081038841 1103 68-69 1.0221599172261264 1103 70-71 0.9331917215370744 1103 72-73 0.8661281798758402 1103 74-75 0.9847416201228789 1103 76-77 1.0050113073419311 1103 78-79 1.0463092037224584 1103 80-81 0.891784010033394 1103 82-83 0.8707344198031386 1103 84-85 0.9977573267884985 1103 86-87 0.9109870530212874 1103 88-89 1.0127824639887173 1103 90-91 0.8764125599622652 1103 92-93 1.0353585213581518 1103 94-95 0.9991639293730863 1103 96-97 0.8989692741474045 1103 98-99 1.415215613253686 1103 100-101 1.9854557074317896 1104 1 0.08527798071852644 1104 2 0.06878763794195208 1104 3 0.02494437829179219 1104 4 0.034516215651279936 1104 5 0.07042777336368289 1104 6 -0.1345305949760487 1104 7 -0.08179181128242163 1104 8 -0.05520961293281346 1104 9 0.12502047714483666 1104 10-11 0.04895859619737308 1104 12-13 -0.004037778769159672 1104 14-15 -0.031340786787076524 1104 16-17 -0.1612352790537841 1104 18-19 -0.17071742289943614 1104 20-21 -0.30516080512816757 1104 22-23 -0.08209645906155316 1104 24-25 -0.21404191359768276 1104 26-27 -0.022449176470516363 1104 28-29 0.11545981986471077 1104 30-31 0.2591452538251886 1104 32-33 0.25665805276356934 1104 34-35 0.21049224470726102 1104 36-37 0.1837772541959808 1104 38-39 0.369150293483699 1104 40-41 0.12130168554048026 1104 42-43 0.3511730172324192 1104 44-45 0.6039766555285695 1104 46-47 0.7412242823770896 1104 48-49 1.1931886534034177 1104 50-51 0.8558832110314754 1104 52-53 0.5794817134821955 1104 54-55 0.7866128865071076 1104 56-57 0.8275230244438418 1104 58-59 0.8569302947812218 1104 60-61 1.1909353406874175 1104 62-63 0.9408566845990123 1104 64-65 0.9293168176411797 1104 66-67 1.2651131533484161 1104 68-69 1.1903781526648132 1104 70-71 1.5085615912839394 1104 72-73 1.2817290646141082 1104 74-75 1.2926698580191633 1104 76-77 1.3146697000674124 1104 78-79 1.5099562914590017 1104 80-81 1.6269867625524377 1104 82-83 1.3536442256522427 1104 84-85 1.2854299736304746 1104 86-87 1.2956614201876668 1104 88-89 1.184927954281175 1104 90-91 1.5274865339116381 1104 92-93 1.232807526027603 1104 94-95 1.2728872029046627 1104 96-97 0.97351338818034 1104 98-99 1.0913719170138236 1104 100-101 1.3596852478594137 1105 1 0.2015780046821014 1105 2 0.22523144335772116 1105 3 0.010201986726976031 1105 4 0.21489484014205829 1105 5 -0.13017610873552599 1105 6 0.09035318168342599 1105 7 0.053017678293421966 1105 8 0.10186683087260207 1105 9 0.2641817520070475 1105 10-11 -0.01157818789081233 1105 12-13 0.018483189359287167 1105 14-15 -0.24506233004132838 1105 16-17 -0.07246461047002839 1105 18-19 -0.07789211736384516 1105 20-21 -0.1666177905103865 1105 22-23 0.19443840794060208 1105 24-25 -0.14514184170695188 1105 26-27 -0.03991383019685202 1105 28-29 0.09739607675423656 1105 30-31 0.08946396937754031 1105 32-33 0.03273761183378099 1105 34-35 0.21378483996247866 1105 36-37 0.12875209244184305 1105 38-39 0.2645684578738283 1105 40-41 -0.009956402166203304 1105 42-43 -0.011630721085346352 1105 44-45 0.34405813168481103 1105 46-47 0.3505940403449799 1105 48-49 0.3857072251743219 1105 50-51 0.4706783224620992 1105 52-53 0.26065592867510645 1105 54-55 0.21893016424495215 1105 56-57 0.33277104744887964 1105 58-59 0.32659240836856895 1105 60-61 0.6270819977686557 1105 62-63 0.4613503342515415 1105 64-65 0.26508005751656327 1105 66-67 0.3149142556728819 1105 68-69 0.0453601799832839 1105 70-71 0.19330637920630167 1105 72-73 0.6570322038424727 1105 74-75 0.49891955847447633 1105 76-77 0.4788201913207111 1105 78-79 0.5960190760264616 1105 80-81 0.7229177474553872 1105 82-83 0.6738407269702371 1105 84-85 0.843019237948706 1105 86-87 0.9530829394783424 1105 88-89 1.0033271874467609 1105 90-91 1.0879370491285059 1105 92-93 0.7174372887882079 1105 94-95 0.48142782116491034 1105 96-97 0.5292430790502145 1105 98-99 0.7262245410253243 1105 100-101 1.0526926765677764 1106 1 -0.13155690414183496 1106 2 -0.08296150463521457 1106 3 0.02330792557253858 1106 4 0.008176186253926687 1106 5 -0.031747630849942254 1106 6 -0.15438360820485997 1106 7 -0.09133615229173131 1106 8 -0.12078531111997393 1106 9 -0.1605062254519254 1106 10-11 -0.17974792021615116 1106 12-13 -0.04144101247812415 1106 14-15 -0.043403501142783796 1106 16-17 0.0684217518134389 1106 18-19 0.11253588430292183 1106 20-21 0.17469220810063746 1106 22-23 0.06913333025740798 1106 24-25 0.1325234955154997 1106 26-27 -0.15594256206581036 1106 28-29 0.02765482231448857 1106 30-31 0.3346670568629122 1106 32-33 0.42611420171016334 1106 34-35 0.25279013789687355 1106 36-37 0.3089413894238078 1106 38-39 0.10021839735434668 1106 40-41 0.28135558068991173 1106 42-43 -0.23844481716249533 1106 44-45 0.3366420156461629 1106 46-47 0.8544285939890486 1106 48-49 0.9561528866224265 1106 50-51 0.5766965378320705 1106 52-53 0.5370662308756238 1106 54-55 0.5525462525041718 1106 56-57 0.7128978407103759 1106 58-59 0.6466412207978749 1106 60-61 0.47997502711563556 1106 62-63 0.4427381152702523 1106 64-65 0.673011085157107 1106 66-67 0.6786065389437042 1106 68-69 0.48889848583482376 1106 70-71 0.4112955452280893 1106 72-73 0.5115820778429132 1106 74-75 -0.027976883774069705 1106 76-77 0.4712106113853949 1106 78-79 0.5596280210033449 1106 80-81 0.678525224090901 1106 82-83 0.524913211443522 1106 84-85 0.6543373719646226 1106 86-87 0.7246864079387692 1106 88-89 0.8228799386025862 1106 90-91 0.9892307769297659 1106 92-93 0.9495493192662146 1106 94-95 0.27635609070476264 1106 96-97 0.6126706640255115 1106 98-99 0.49317495474042516 1106 100-101 0.6295970557966974 1107 1 -0.03792239914852047 1107 2 0.09750558665990638 1107 3 0.019749696430444885 1107 4 -0.20012765899259222 1107 5 0.030000422936332427 1107 6 -0.02713268424480475 1107 7 0.03801825519430935 1107 8 0.03385021612701422 1107 9 0.20424174969944175 1107 10-11 0.3098132970520737 1107 12-13 0.20100020793549334 1107 14-15 0.14296120751492225 1107 16-17 -0.025822173640676738 1107 18-19 0.008138227622886518 1107 20-21 0.03200918537515207 1107 22-23 -0.10580965944141241 1107 24-25 0.002097687541919413 1107 26-27 0.025694223339556288 1107 28-29 -0.11239393132237296 1107 30-31 0.0782288245754259 1107 32-33 0.14448331392215863 1107 34-35 -0.3947879072395537 1107 36-37 -0.15353993478787942 1107 38-39 -0.4187129543795507 1107 40-41 -0.2125207266152671 1107 42-43 -0.15419735313795258 1107 44-45 -0.4372531640345798 1107 46-47 -0.7177975599780879 1107 48-49 -0.40920450898974536 1107 50-51 -0.128029419547822 1107 52-53 0.015559586226736144 1107 54-55 -0.05654000997912334 1107 56-57 -0.4099888464013546 1107 58-59 -0.21154074035648307 1107 60-61 -0.0011919147731660473 1107 62-63 -0.16980808273242332 1107 64-65 -0.0403745403835174 1107 66-67 0.0633277782297057 1107 68-69 -0.374090033470047 1107 70-71 -0.34807183701614974 1107 72-73 -0.4752367473517083 1107 74-75 -0.47028835660571033 1107 76-77 -0.356864013132963 1107 78-79 -0.13423649106711366 1107 80-81 0.05198201421435655 1107 82-83 0.029788575129924055 1107 84-85 -0.03209614222897628 1107 86-87 -0.047292046099133955 1107 88-89 -0.2832771739239561 1107 90-91 -0.09527225051381549 1107 92-93 0.007935731155807701 1107 94-95 -0.05817642482562846 1107 96-97 0.26818100451462 1107 98-99 0.33426365490556265 1107 100-101 0.5683699582107913 1108 1 0.08674916259787224 1108 2 3.238568251120455E-4 1108 3 0.014143750348310391 1108 4 0.16117241659319603 1108 5 -0.05851593598818994 1108 6 -0.12095804507696784 1108 7 -0.040195040706059615 1108 8 -0.1146758609704932 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-0.42389397960473474 2316 58-59 -0.650559583761293 2316 60-61 -0.8229917848186545 2316 62-63 -1.1590218579111067 2316 64-65 -0.9959463402535604 2316 66-67 -0.722214781874257 2316 68-69 -0.6140348028007736 2316 70-71 -0.9132602710642352 2316 72-73 -0.9297689110943992 2316 74-75 -0.7693949193087697 2316 76-77 -0.9452493282726664 2316 78-79 -0.784740064816301 2316 80-81 -0.726737933803836 2316 82-83 -1.0750197419590926 2316 84-85 -1.0492663826448343 2316 86-87 -1.1558365555208354 2316 88-89 -1.235031559888867 2316 90-91 -0.7749831017158897 2316 92-93 -0.569257251300332 2316 94-95 -0.2019710966904782 2316 96-97 -0.19353114623261902 2316 98-99 -0.22660379148820198 2316 100-101 -1.3225819586183185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 5.0 6 12.0 7 42.0 8 107.0 9 176.0 10 324.0 11 502.0 12 823.0 13 1142.0 14 1355.0 15 2106.0 16 2878.0 17 3874.0 18 3341.0 19 1943.0 20 1649.0 21 1671.0 22 1749.0 23 1749.0 24 1990.0 25 2116.0 26 2475.0 27 2938.0 28 3592.0 29 4441.0 30 5651.0 31 6990.0 32 8845.0 33 11373.0 34 15724.0 35 22296.0 36 34533.0 37 56758.0 38 55842.0 39 6478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.96341969725872 23.710993166440378 17.272785077630285 19.05280205867062 2 14.187660985521116 25.12327425390571 37.62939590942567 23.059668851147507 3 19.450228604113004 28.030640054132256 32.98590991166124 19.533221430093498 4 11.138983076192648 20.320157910674297 41.85791777728763 26.682941235845426 5 10.14055436526187 41.337715608302396 36.514969656858895 12.006760369576842 6 25.624969625373375 41.656043335713456 17.14661692081662 15.572370118096549 7 22.606946723839503 36.59721936648809 24.49746348502578 16.298370424646627 8 23.444725656372317 33.127222020763156 22.234226690044228 21.1938256328203 9 22.912375277110055 19.228166718381416 25.608146755242196 32.25131124926634 10-11 22.189178782248508 28.470838489231493 29.123752771100552 20.216229957419447 12-13 25.09117418896563 27.093735104165695 29.12265288383352 18.692437823035153 14-15 21.535142975704037 29.388993356835506 29.867510551677988 19.20835311578247 16-17 17.50195332214301 32.1019615466573 30.783048528372703 19.613036602826988 18-19 17.08082080652578 31.73522297779755 33.11974518959374 18.064211026082923 20-21 18.892083157316268 28.981506058102458 32.49580362850617 19.63060715607511 22-23 18.599399983177097 28.598745759226894 33.36467377590026 19.437180481695748 24-25 20.495154069740273 29.41503032795312 32.004822565118644 18.084993037187957 26-27 18.75813108429411 28.072054491349274 35.18366904430787 17.98614538004875 28-29 17.62188978467992 30.886534036424408 33.62275712141406 17.86881905748162 30-31 18.782547580684355 29.049919063302593 32.80291446878984 19.364618887223216 32-33 18.39450004299178 29.622270489321217 33.796772251984166 18.18645721570284 34-35 19.758647889851325 29.288616898386127 32.56795504929101 18.384780162471543 36-37 18.870400346924967 28.410463077538477 34.00201126758457 18.717125307951985 38-39 18.151689950765068 30.250324307551974 33.3352648480521 18.262720893630863 40-41 17.840466853338217 30.449955699775323 33.37302284545764 18.336554601428823 42-43 17.847648319106217 30.189532879411622 31.2527341865865 20.71008461489566 44-45 19.00479638719518 28.72542459055003 31.891675670017534 20.378103352237254 46-47 20.75493564317571 30.306213620543343 31.57334958297974 17.365501153301206 48-49 19.611167395034638 29.87797811531517 31.46474861024401 19.046105879406188 50-51 17.378585607847683 30.354626102365295 33.031518581794664 19.23526970799236 52-53 18.878437940432086 31.79036460767198 30.053870568575626 19.277326883320313 54-55 18.96143076641258 29.62526122178898 30.821928050453657 20.59137996134478 56-57 20.487265087310696 29.66301921919452 29.520959426975658 20.328756266519125 58-59 21.090832283461623 29.08618169447425 30.4234129491239 19.39957307294023 60-61 19.803882718426276 30.68566280239109 31.5604520492125 17.95000242997013 62-63 18.2909459312954 30.67837289200091 31.91429308430501 19.11638809239868 64-65 18.558429566381175 31.62680892584105 30.558743593290295 19.256017914487483 66-67 19.363497362547804 30.229763021836085 28.976646117842336 21.430093497773772 68-69 18.644786966387905 29.43310666073505 31.010718037481354 20.911388335395692 70-71 20.862415091236034 29.643392537374808 29.864145977651752 19.63004639373741 72-73 19.33680383562156 28.34490686654766 32.434367318709874 19.88392197912091 74-75 18.373377994938185 31.824571110272043 29.760778786734605 20.041272108055164 76-77 18.294123584542398 29.50525808151989 30.779310112788 21.42130822114971 78-79 18.56403718975824 29.360768319170973 31.262126485552894 20.813068005517902 80-81 20.12688182494495 29.577409502304736 29.623578934775864 20.67212973797445 82-83 19.08778921317567 28.9299159230335 31.42773829595541 20.55455656783542 84-85 18.373568858357867 29.475396046544233 30.800504696481145 21.35053039861676 86-87 17.964395329971254 30.190509658196664 30.4450957595152 21.399999252316885 88-89 18.291693614412342 29.525258604898074 29.284504641242947 22.89854313944664 90-91 18.732170031070414 27.642948212235986 30.68530642308857 22.939575333605028 92-93 20.171408276944934 28.990429548768176 30.56132939549142 20.27683277879547 94-95 18.758060958604524 27.967460830750714 31.108290684242206 22.16618752640256 96-97 17.073904737694072 28.631403438594656 32.017660275222156 22.27703154848912 98-99 18.056734194913513 29.517221011390955 29.465630876321995 22.96041391737354 100-101 17.183985941428855 28.16201007655565 30.523737859993833 24.130266122021666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 448.0 1 296.0 2 148.5 3 166.5 4 149.5 5 121.5 6 198.5 7 339.5 8 473.5 9 561.5 10 507.0 11 494.0 12 542.5 13 555.5 14 600.5 15 608.5 16 617.5 17 676.5 18 736.5 19 745.5 20 790.5 21 924.0 22 1055.0 23 1160.5 24 1267.0 25 1601.5 26 2249.5 27 2928.0 28 3121.5 29 3518.0 30 4400.0 31 5165.0 32 6636.0 33 8476.0 34 9702.5 35 11230.5 36 13961.5 37 16022.0 38 15501.0 39 13564.0 40 11922.0 41 10230.5 42 8146.5 43 7768.5 44 8325.0 45 8185.0 46 7615.0 47 10980.0 48 18674.0 49 16822.5 50 8143.0 51 3988.0 52 2537.0 53 1977.0 54 1644.5 55 1429.0 56 1193.5 57 1015.5 58 950.0 59 872.5 60 739.5 61 620.0 62 460.5 63 292.0 64 195.5 65 162.0 66 128.0 67 95.0 68 81.0 69 69.0 70 42.0 71 27.5 72 20.0 73 21.5 74 18.5 75 19.0 76 18.0 77 10.5 78 7.5 79 4.5 80 2.0 81 2.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050842451952013694 2 0.0 3 0.0 4 0.0 5 0.019439761040475824 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003925336363942234 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.8692077923534447E-4 24-25 1.8692077923534447E-4 26-27 3.7384155847068894E-4 28-29 0.002803811688530167 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01719671168965169 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.8692077923534447E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0020561285715887893 86-87 0.0 88-89 0.0 90-91 0.013458296104944801 92-93 0.0011215246754120668 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.015140583118062904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267493.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.65805086488244 #Duplication Level Percentage of deduplicated Percentage of total 1 80.79855308042767 26.387232563095107 2 7.829849584468509 5.114152519879025 3 2.849195265459374 2.7914749171006346 4 1.6266398040248173 2.124915418347396 5 0.9981913505345817 1.6299491949322036 6 0.7440646534948144 1.457982078035687 7 0.5506078435861627 1.2587245273708096 8 0.44185993269076673 1.1544227325574874 9 0.38691361981730354 1.1372260208678358 >10 3.0861512397261843 20.929519650981522 >50 0.4109526316994436 9.318000844881922 >100 0.248403122782115 12.88295394645841 >500 0.011447148515304838 2.8165223015181704 >1k 0.016026007921426774 9.092948226682568 >5k 0.0011447148515304837 1.9039750572912189 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5093 1.9039750572912189 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3438 1.2852672780222287 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2618 0.9787172000762636 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.8392742987666967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2076 0.7760950753851503 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1825 0.6822608442090073 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1680 0.6280538182307573 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1605 0.6000157013454558 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1346 0.5031907377015473 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1323 0.49459238185672144 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1290 0.48225561042718873 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1271 0.47515262081624565 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1267 0.4736572545823629 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1257 0.46991883899765596 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1082 0.40449656626528546 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 978 0.3656170441843338 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 946 0.3536541143132717 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 920 0.34393423379303384 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 899 0.33608356106514936 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 883 0.3301020961296183 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 652 0.24374469612288918 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 622 0.23252944936876851 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 621 0.23215560781029784 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 512 0.19140687793699274 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 501 0.18729462079381518 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.18505157144299103 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 491 0.1835562052091083 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 386 0.14430284156968592 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 362 0.1353306441663894 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 360 0.13458296104944803 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 357 0.13346143637403596 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 347 0.12972302078932907 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 328 0.12262003117838598 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 317 0.1185077740352084 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 314 0.11738624935979634 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 303 0.11327399221661875 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 291 0.1087878935149705 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 285 0.10654484416414635 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 279 0.10430179481332222 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 274 0.10243258702096877 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 0.10205874546249807 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.8692077923534447E-4 0.0 14-15 0.0 0.0 0.0 3.7384155847068894E-4 0.0 16-17 0.0 0.0 0.0 7.476831169413779E-4 0.0 18-19 0.0 0.0 0.0 7.476831169413779E-4 0.0 20-21 0.0 0.0 0.0 7.476831169413779E-4 0.0 22-23 0.0 0.0 0.0 7.476831169413779E-4 0.0 24-25 0.0 0.0 0.0 0.0022430493508241336 0.0 26-27 0.0 0.0 0.0 0.002429970130059478 0.0 28-29 0.0 0.0 0.0 0.005046861039354301 0.0 30-31 0.0 0.0 0.0 0.013084454546474113 0.0 32-33 0.0 0.0 0.0 0.019813602598946517 0.0 34-35 0.0 0.0 0.0 0.028038116885301674 0.0 36-37 0.0 0.0 0.0 0.037384155847068896 0.0 38-39 0.0 0.0 0.0 0.044300224678776645 0.0 40-41 0.0 0.0 0.0 0.058506203900662815 0.0 42-43 0.0 0.0 0.0 0.07962825195425674 0.0 44-45 0.0 0.0 0.0 0.12579768442538683 0.0 46-47 0.0 0.0 0.0 0.21963191560152975 0.0 48-49 0.0 0.0 0.0 0.27869888183989866 0.0 50-51 0.0 0.0 0.0 0.3530933519755657 0.0 52-53 0.0 0.0 0.0 0.44449761302164914 0.0 54-55 0.0 0.0 0.0 0.5609492584852688 0.0 56-57 0.0 0.0 0.0 0.6874946260275969 0.0 58-59 0.0 0.0 0.0 0.8732938805875294 0.0 60-61 0.0 0.0 0.0 1.0817105494349386 0.0 62-63 0.0 0.0 0.0 1.3248944832201217 0.0 64-65 0.0 0.0 0.0 1.5493863390817704 0.0 66-67 0.0 0.0 0.0 1.8815445637829775 0.0 68-69 0.0 0.0 0.0 2.296321772906207 0.0 70-71 0.0 0.0 0.0 2.6129655729308805 0.0 72-73 0.0 0.0 0.0 3.0785852340061233 0.0 74-75 0.0 0.0 0.0 3.549438676899956 0.0 76-77 0.0 0.0 0.0 3.955056767840654 0.0 78-79 0.0 0.0 0.0 4.515258343208981 0.0 80-81 0.0 0.0 0.0 4.996579349739993 0.0 82-83 0.0 0.0 0.0 5.584445200435152 0.0 84-85 0.0 0.0 0.0 6.328202981012588 0.0 86-87 0.0 0.0 0.0 7.2168243654974145 0.0 88-89 0.0 0.0 0.0 8.335545229220951 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGAAG 15 6.6101016E-4 95.00804 9 TCACGCA 25 0.005011682 57.015484 4 AGTACTA 25 0.005011682 57.015484 2 ATACTCG 40 5.383438E-4 47.49514 6 TACTCGC 45 9.607806E-4 42.225796 7 CTCGCCT 55 0.0025750326 34.548378 9 ACTCGCC 55 0.0025750326 34.548378 8 GGGTTGC 55 0.0025773954 34.54192 6 CATGGGG 275 0.0 32.8271 4 ATCAGCG 30 0.003945637 31.67231 42-43 ACAATGC 75 3.070656E-4 31.669346 8 GACTATC 30 0.0039510587 31.66343 56-57 CTTACTA 110 2.7213227E-6 30.229832 9 ACTACAA 65 0.0058284164 29.23871 5 ACCCTTG 170 3.610694E-9 27.943542 7 CAAACGT 35 0.008380342 27.147692 84-85 TCAGCGG 35 0.008380342 27.147692 42-43 ATGGGGG 110 9.432549E-5 25.91613 5 ATATAGG 130 9.9625795E-6 25.583872 2 CCCTTGG 195 1.556873E-8 24.361034 8 >>END_MODULE