##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937508_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 297829 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.813084018010336 33.0 31.0 34.0 30.0 34.0 2 31.907131273314555 33.0 31.0 34.0 30.0 34.0 3 31.925312847304998 33.0 31.0 34.0 30.0 34.0 4 35.53363171484308 37.0 35.0 37.0 33.0 37.0 5 35.56779897189327 37.0 35.0 37.0 33.0 37.0 6 35.63676136306404 37.0 35.0 37.0 33.0 37.0 7 35.5543785192174 37.0 35.0 37.0 33.0 37.0 8 35.56917895839559 37.0 35.0 37.0 33.0 37.0 9 37.173552609047476 39.0 37.0 39.0 34.0 39.0 10-11 37.086237404685235 39.0 37.0 39.0 33.5 39.0 12-13 37.00552330364068 39.0 37.0 39.0 33.0 39.0 14-15 38.08876905875519 40.0 38.0 41.0 33.0 41.0 16-17 38.06444469813215 40.0 38.0 41.0 33.0 41.0 18-19 38.13733048158507 40.0 38.0 41.0 33.5 41.0 20-21 38.048950908071404 40.0 38.0 41.0 33.0 41.0 22-23 37.91641512411484 40.0 38.0 41.0 33.0 41.0 24-25 37.84907782653805 40.0 37.5 41.0 33.0 41.0 26-27 37.856229581404094 40.0 37.5 41.0 33.0 41.0 28-29 37.81379919349693 40.0 37.0 41.0 33.0 41.0 30-31 37.6305010593327 40.0 37.0 41.0 33.0 41.0 32-33 37.322621369980766 39.5 36.0 41.0 32.5 41.0 34-35 37.2549567033432 39.5 36.0 41.0 32.5 41.0 36-37 37.077250704263186 39.5 36.0 41.0 31.5 41.0 38-39 36.94769649698317 39.0 36.0 41.0 31.0 41.0 40-41 36.82066051324753 39.5 36.0 41.0 31.0 41.0 42-43 36.49428027492286 39.0 35.0 41.0 30.0 41.0 44-45 36.1808470632477 39.0 35.0 41.0 30.0 41.0 46-47 35.73318414257846 39.0 35.0 41.0 28.5 41.0 48-49 35.313191462214895 39.0 35.0 41.0 26.5 41.0 50-51 34.28936403103795 38.0 34.0 39.5 24.0 40.5 52-53 34.443924198113685 38.0 34.5 39.5 24.0 40.5 54-55 34.87114921649672 39.0 35.0 40.0 24.0 41.0 56-57 34.62457987637201 39.0 35.0 40.0 22.5 41.0 58-59 34.39981331569457 39.0 34.5 40.0 22.0 41.0 60-61 34.03415886297171 38.0 34.0 40.0 19.5 41.0 62-63 33.76250465871355 38.0 34.0 40.0 18.0 41.0 64-65 33.38157130433907 37.5 33.5 40.0 14.0 41.0 66-67 32.9608164416494 37.0 33.0 39.5 10.0 41.0 68-69 32.46222328920287 36.5 32.0 39.0 7.5 41.0 70-71 31.9314086270981 36.0 31.5 39.0 2.0 40.0 72-73 31.447431244103157 35.5 31.0 38.0 2.0 40.0 74-75 30.984145264564567 35.0 31.0 37.0 2.0 39.5 76-77 30.553144589680656 35.0 31.0 37.0 2.0 39.0 78-79 30.037032995443695 34.5 30.0 36.0 2.0 39.0 80-81 29.495384935650996 34.0 29.0 36.0 2.0 38.0 82-83 29.01021223588032 34.0 29.0 35.5 2.0 37.0 84-85 28.46622054937565 34.0 28.5 35.0 2.0 37.0 86-87 28.12854356023087 34.0 28.5 35.0 2.0 36.0 88-89 27.624885420828733 34.0 26.5 35.0 2.0 36.0 90-91 27.125627457366477 33.0 26.0 35.0 2.0 35.5 92-93 26.661016892243538 33.0 25.0 35.0 2.0 35.0 94-95 26.149236642502913 33.0 24.5 35.0 2.0 35.0 96-97 25.445054041077263 32.0 21.5 35.0 2.0 35.0 98-99 24.650250647183448 32.0 10.0 34.0 2.0 35.0 100-101 22.141341172283425 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1853937996899404 1101 2 0.1950108812521485 1101 3 -0.11501962008195221 1101 4 -0.22784586033423437 1101 5 0.044462313346897986 1101 6 0.12783955093287602 1101 7 0.0357649803514164 1101 8 0.05117580002039546 1101 9 -0.1121885210895428 1101 10-11 -0.12518966214369698 1101 12-13 0.07127201494198943 1101 14-15 -0.12926064005383608 1101 16-17 -0.12585194434209512 1101 18-19 -0.15808055266454346 1101 20-21 0.24258145808273213 1101 22-23 -0.1078101867711112 1101 24-25 -0.09919929032621866 1101 26-27 -0.245157836435709 1101 28-29 -0.050451008110599105 1101 30-31 0.22719562021939765 1101 32-33 0.22172082441154117 1101 34-35 0.08310756214454784 1101 36-37 0.0806653536972135 1101 38-39 0.31438009638418407 1101 40-41 0.3306731685979827 1101 42-43 0.5467970564301794 1101 44-45 1.074613664156935 1101 46-47 1.1944792709226917 1101 48-49 0.8470215247499482 1101 50-51 0.77419233193514 1101 52-53 0.9018042675656446 1101 54-55 0.8471825669092752 1101 56-57 0.8053326821202447 1101 58-59 0.8445139254719365 1101 60-61 0.8520308280271038 1101 62-63 0.7477091908660825 1101 64-65 0.7023895680931176 1101 66-67 0.6991520096506605 1101 68-69 0.4577781350160066 1101 70-71 0.7686293010340215 1101 72-73 0.6016676496022164 1101 74-75 0.7722527635292025 1101 76-77 0.835941794452598 1101 78-79 0.7381532243976388 1101 80-81 0.8045182341105637 1101 82-83 0.9494872245655905 1101 84-85 1.104352327863456 1101 86-87 0.8649487403846123 1101 88-89 0.33056458746800743 1101 90-91 0.3998782302539574 1101 92-93 0.9789919908703126 1101 94-95 1.2473742555146146 1101 96-97 1.127674501284158 1101 98-99 1.2441395462938338 1101 100-101 1.1238723042987964 1102 1 0.10530721960336109 1102 2 0.07942646566772638 1102 3 0.08498037991804708 1102 4 -0.1414822239705984 1102 5 0.016107334991922073 1102 6 -0.013502440409112637 1102 7 0.2786221232085637 1102 8 0.3617818606264578 1102 9 0.4949543360533113 1102 10-11 0.19439908244505233 1102 12-13 0.2912936599636353 1102 14-15 0.1514752906820931 1102 16-17 0.22728658379643463 1102 18-19 0.07536100577701887 1102 20-21 0.3317589472602194 1102 22-23 0.16502531106439022 1102 24-25 0.234891618764685 1102 26-27 0.2310326397547655 1102 28-29 0.10312042046082581 1102 30-31 -0.1504017823779975 1102 32-33 -0.3559848032940849 1102 34-35 -0.07782317378619297 1102 36-37 0.04354414157599962 1102 38-39 -0.28854198153788957 1102 40-41 0.08738312530794445 1102 42-43 0.2393295239626454 1102 44-45 0.3322976468409209 1102 46-47 0.7765139029573263 1102 48-49 0.2403115680399921 1102 50-51 0.23068583842865564 1102 52-53 0.2948778606392395 1102 54-55 0.4535678482945542 1102 56-57 0.5301162319037971 1102 58-59 0.6145355704935795 1102 60-61 0.7339572349535075 1102 62-63 0.684289277446176 1102 64-65 0.5825843732879292 1102 66-67 -0.043488682990030725 1102 68-69 0.10171752895539754 1102 70-71 -0.07563476823004933 1102 72-73 -0.18750983957528078 1102 74-75 0.1897852310616699 1102 76-77 0.34968638319718437 1102 78-79 0.2618545230989362 1102 80-81 0.6559251605174907 1102 82-83 0.7245954496738136 1102 84-85 1.1085731070842364 1102 86-87 0.7440612944971683 1102 88-89 0.4062139381173573 1102 90-91 0.5748782302539581 1102 92-93 0.6018274887058084 1102 94-95 0.4589543420947031 1102 96-97 0.7652286138382713 1102 98-99 0.746412273566559 1102 100-101 0.5286342090607015 1103 1 0.2495046114787698 1103 2 0.20076054954051514 1103 3 0.1730468026593961 1103 4 0.10926362460806871 1103 5 0.5311837961984125 1103 6 0.3196995485204255 1103 7 0.3904149743203007 1103 8 0.40137281662608615 1103 9 0.5447713920623158 1103 10-11 0.16266064544345937 1103 12-13 0.24986857318251054 1103 14-15 0.2780912959358659 1103 16-17 0.6270379676035489 1103 18-19 0.2671285261823044 1103 20-21 0.36127391114050766 1103 22-23 0.21129137729948866 1103 24-25 0.16518995819615157 1103 26-27 0.15487633991426009 1103 28-29 0.2659264998303712 1103 30-31 0.12747606732647654 1103 32-33 0.17490927682599988 1103 34-35 0.07528218318913815 1103 36-37 0.12228511164823885 1103 38-39 0.02624833650320113 1103 40-41 0.4542608811949833 1103 42-43 0.26091691489988733 1103 44-45 0.3762651860096966 1103 46-47 0.4924065758159415 1103 48-49 0.4877878791385939 1103 50-51 0.8538634348260672 1103 52-53 0.592799804536412 1103 54-55 0.7207552017048187 1103 56-57 0.693269435769075 1103 58-59 0.8532540243823163 1103 60-61 1.122581871410965 1103 62-63 0.7323800927196444 1103 64-65 0.4865809958604004 1103 66-67 0.44499053644894104 1103 68-69 0.458411402677136 1103 70-71 0.170176283463352 1103 72-73 0.16676354441946728 1103 74-75 0.1167202155817968 1103 76-77 0.07398509789837604 1103 78-79 0.17093135957515315 1103 80-81 -0.05447919261131773 1103 82-83 -0.18507712749102367 1103 84-85 -0.12919779074858084 1103 86-87 0.043686024772053855 1103 88-89 0.2402133752127682 1103 90-91 -0.018606149143735706 1103 92-93 -0.18874855032224502 1103 94-95 -0.3313167902816936 1103 96-97 0.4271950271978753 1103 98-99 0.526541741621724 1103 100-101 1.0499307659609762 1104 1 -0.13529153906768343 1104 2 0.02203325646729226 1104 3 -0.2527523692604845 1104 4 0.012570349961492866 1104 5 0.06053960954467641 1104 6 -0.05362657330433507 1104 7 0.19203942879454416 1104 8 0.02160296321864763 1104 9 -0.1058123671230291 1104 10-11 0.0358375636425734 1104 12-13 -0.0031294871703551053 1104 14-15 -0.14125015656142637 1104 16-17 -0.10177511755442481 1104 18-19 -0.2064297937848707 1104 20-21 0.061076216336523714 1104 22-23 -0.11440148703711372 1104 24-25 -0.018969279373322934 1104 26-27 -0.06944198602106155 1104 28-29 -0.34531879093019313 1104 30-31 -0.25152445488621566 1104 32-33 -0.2038581147216192 1104 34-35 -0.28389290726556027 1104 36-37 -0.13346389055251962 1104 38-39 -0.1946756069486284 1104 40-41 -0.035681861913673174 1104 42-43 -0.025922392795294513 1104 44-45 0.039955551185570926 1104 46-47 0.5052287623950775 1104 48-49 0.7640337294127519 1104 50-51 0.3418851812544972 1104 52-53 0.3626671998141191 1104 54-55 0.430502861072938 1104 56-57 0.047157122739868385 1104 58-59 0.40661297100471216 1104 60-61 0.48522594616198234 1104 62-63 0.3277631730285009 1104 64-65 0.46856465806339287 1104 66-67 -0.021783681164549762 1104 68-69 0.3726193179294697 1104 70-71 0.4930855676589623 1104 72-73 0.6963711388840146 1104 74-75 0.5680984840737189 1104 76-77 0.35769112945494186 1104 78-79 0.34586328542095046 1104 80-81 0.2122595891410768 1104 82-83 0.33158705244855113 1104 84-85 0.5733321432288143 1104 86-87 0.6693807541539769 1104 88-89 0.26582511007792675 1104 90-91 0.5677314613602 1104 92-93 0.8549308110862412 1104 94-95 0.2806045794075871 1104 96-97 0.6330727175257493 1104 98-99 1.251806578057252 1104 100-101 1.6117849940187163 1105 1 0.28267974727528866 1105 2 -0.03766662088643358 1105 3 -0.10440448671232616 1105 4 0.06597558767882816 1105 5 -0.24585964014870854 1105 6 0.23906515167943354 1105 7 -0.03123089802170398 1105 8 0.03879147775583647 1105 9 0.3155858003973506 1105 10-11 -0.10136393697065671 1105 12-13 -0.06498469242250593 1105 14-15 -0.09064955054454771 1105 16-17 0.028193860152811112 1105 18-19 0.14239965574979863 1105 20-21 0.007707051052626923 1105 22-23 0.21580556873087176 1105 24-25 -0.054221064958774434 1105 26-27 -0.15823428094928005 1105 28-29 -0.10126172434047476 1105 30-31 0.03179745550986013 1105 32-33 -0.24067905116198318 1105 34-35 -0.09078815291883302 1105 36-37 0.08086949087739725 1105 38-39 0.20046182902279952 1105 40-41 -0.06445864569550253 1105 42-43 -0.05745871773577704 1105 44-45 -0.07074172895294595 1105 46-47 0.04856979118088134 1105 48-49 0.11381909843375126 1105 50-51 -0.07219930038463218 1105 52-53 -0.21706189621070138 1105 54-55 -0.3557589526490119 1105 56-57 -0.09319169769155877 1105 58-59 0.10088106133009234 1105 60-61 0.06282676861345493 1105 62-63 -0.37030154091710443 1105 64-65 -0.5243290725476797 1105 66-67 -0.5272847272651191 1105 68-69 -0.6216992738027258 1105 70-71 -0.6950051184387256 1105 72-73 -0.5950039422789608 1105 74-75 -0.5506339306149748 1105 76-77 -0.6239209475957743 1105 78-79 -0.34059512306328443 1105 80-81 -0.518008789763762 1105 82-83 -0.36509789091773115 1105 84-85 -0.3671753959097792 1105 86-87 -0.13195031864019668 1105 88-89 0.056758303931186305 1105 90-91 0.2942032166448101 1105 92-93 0.7023083451746892 1105 94-95 0.051995532441278414 1105 96-97 0.6761522211690654 1105 98-99 0.5265973579432632 1105 100-101 1.4499279851448996 1106 1 -0.3795412652451233 1106 2 -0.06602807978681469 1106 3 0.03952583446350033 1106 4 -0.020270102758480846 1106 5 0.15853157741616286 1106 6 0.04407331716664231 1106 7 -0.19107484648840511 1106 8 -0.3321575333129374 1106 9 -0.3141365730375938 1106 10-11 -0.7101463721003967 1106 12-13 -0.37082755215757857 1106 14-15 -0.48488834568154715 1106 16-17 -0.2727134162035654 1106 18-19 -0.383729903313899 1106 20-21 -0.2152107497094775 1106 22-23 -0.048611052571978064 1106 24-25 -0.21662353275046797 1106 26-27 -0.4841188753967458 1106 28-29 0.08039314773355954 1106 30-31 -0.3716139035901236 1106 32-33 -0.23174237905165995 1106 34-35 0.13429803833502518 1106 36-37 -0.33675888872703297 1106 38-39 -0.05369349668940515 1106 40-41 -0.3701926322678162 1106 42-43 -0.19097350634039145 1106 44-45 -0.08436901982575051 1106 46-47 -0.19924367280025024 1106 48-49 0.24182671955514223 1106 50-51 -0.05719294945014042 1106 52-53 -0.0733039575425849 1106 54-55 -0.27067457594786504 1106 56-57 -0.20473225294468733 1106 58-59 0.15847496443297615 1106 60-61 -0.02513367413740042 1106 62-63 -0.5990440558871626 1106 64-65 -0.4295368388332861 1106 66-67 -0.23894322844457605 1106 68-69 -0.6376764104385444 1106 70-71 -0.2998771924724757 1106 72-73 -0.01023711230255131 1106 74-75 -0.11021476893833082 1106 76-77 0.028474261985063976 1106 78-79 0.22397573522014724 1106 80-81 0.25137970597203463 1106 82-83 0.37004999512835823 1106 84-85 0.09342159193272082 1106 86-87 0.08497038540625823 1106 88-89 0.5592442411476597 1106 90-91 0.8809388363145629 1106 92-93 0.9881911250694451 1106 94-95 1.149863433003791 1106 96-97 1.0621983108079682 1106 98-99 1.3312607584150449 1106 100-101 1.6104523908788835 1107 1 -0.25727512558767884 1107 2 -0.12755640653253408 1107 3 -0.21343859241396856 1107 4 0.1442885270175438 1107 5 -0.18784523417804166 1107 6 0.007050919274675493 1107 7 -0.27816312053320047 1107 8 -0.10936438443282981 1107 9 -0.04681114484260718 1107 10-11 -0.14710289383953068 1107 12-13 -0.26555745993096735 1107 14-15 -0.011238806814610314 1107 16-17 -0.25275294189526676 1107 18-19 -0.1642305620754243 1107 20-21 0.00712126610079622 1107 22-23 0.13597392766517657 1107 24-25 0.17369267278313316 1107 26-27 0.12398389140167154 1107 28-29 0.12018234404449402 1107 30-31 0.26540849430184466 1107 32-33 0.20646578959128448 1107 34-35 -0.12163081541991971 1107 36-37 -0.22457180045298486 1107 38-39 -0.32049191566173363 1107 40-41 -0.41821634768282934 1107 42-43 -0.02219880278307329 1107 44-45 0.1774228115313008 1107 46-47 -0.17710928544847349 1107 48-49 -0.3768821078493332 1107 50-51 -0.28703484668334056 1107 52-53 -0.2825266189391513 1107 54-55 -0.34478524788463005 1107 56-57 -0.30051617916340234 1107 58-59 -0.15423913306373294 1107 60-61 0.10398358541464603 1107 62-63 -0.3388991283509313 1107 64-65 -0.054833281521034394 1107 66-67 0.2117155330837477 1107 68-69 0.22648696242048771 1107 70-71 0.16454968499788336 1107 72-73 0.36426881655120624 1107 74-75 0.20427798468486102 1107 76-77 0.20963368227491586 1107 78-79 -0.07747354014216867 1107 80-81 -0.07082055753257421 1107 82-83 0.2779551334682786 1107 84-85 -0.10736892190127278 1107 86-87 -0.05290840247252859 1107 88-89 0.1487699328472658 1107 90-91 0.06967401418017616 1107 92-93 -0.24171664831659 1107 94-95 0.20651692443989234 1107 96-97 0.26140779697397676 1107 98-99 0.6165045001805289 1107 100-101 0.8331137874533248 1108 1 -0.1605573080258722 1108 2 -0.2801992027814677 1108 3 -0.140420689600667 1108 4 -0.27463730418450183 1108 5 -0.18754685395638404 1108 6 -0.559402618662233 1108 7 -0.3323404436363546 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-0.30672645241940444 2316 46-47 0.21168450663186889 2316 48-49 0.03252106458020165 2316 50-51 -0.593687819409574 2316 52-53 -0.21980837176352708 2316 54-55 -0.19805467616261296 2316 56-57 -0.5623557318280135 2316 58-59 -1.1447104328469138 2316 60-61 -1.48457056270815 2316 62-63 -0.8789653157296797 2316 64-65 -1.2580741807564522 2316 66-67 -1.1412457815345327 2316 68-69 -1.4915776273318855 2316 70-71 -1.2015235775834263 2316 72-73 -1.2073619035218321 2316 74-75 -0.990529729568248 2316 76-77 -1.0085336120306856 2316 78-79 -0.3970216401079334 2316 80-81 -0.3941344911169651 2316 82-83 -0.8533811668854803 2316 84-85 -0.5780410661441131 2316 86-87 -0.4796800560158374 2316 88-89 -0.6010874219399085 2316 90-91 -0.5603043037474755 2316 92-93 -1.5109737520481872 2316 94-95 -1.9463188623911059 2316 96-97 -2.308476945694057 2316 98-99 -2.045141054994641 2316 100-101 -1.8376268264916717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1184.0 3 250.0 4 154.0 5 153.0 6 164.0 7 203.0 8 269.0 9 343.0 10 470.0 11 678.0 12 1078.0 13 1580.0 14 1733.0 15 2427.0 16 3138.0 17 4386.0 18 3651.0 19 2168.0 20 2135.0 21 2128.0 22 2385.0 23 2473.0 24 2788.0 25 3136.0 26 3413.0 27 3902.0 28 4528.0 29 5530.0 30 6685.0 31 8493.0 32 10726.0 33 14009.0 34 19000.0 35 26770.0 36 38252.0 37 57967.0 38 52537.0 39 6943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72478160609988 23.333389439666057 16.603659255020116 19.33816969921395 2 14.03677628656427 24.78024418808136 38.399720004846074 22.7832595205083 3 18.99544731095236 28.2764723894383 33.23362058232695 19.494459717282382 4 10.819760398438552 19.839480414591463 42.44514739534257 26.895611791627406 5 9.987313103847463 40.99852939691813 36.61296890196092 12.401188597273496 6 25.285793151736303 41.73084950247081 17.481856006032288 15.501501339760592 7 22.392817709315164 36.32213933697789 24.81990412840331 16.465138825303637 8 22.900658016526144 33.020649265411215 22.409585836657076 21.669106881405565 9 22.901184706515888 19.32619816908993 25.513260635433493 32.25935648896069 10-11 21.950685890563808 28.244116790728906 29.377927299471047 20.42727001923624 12-13 25.023730502278802 27.28132594602236 28.971072349420705 18.72387120227813 14-15 21.49354998300413 29.638947131063404 29.658467140756095 19.20903574517637 16-17 17.48497491471339 32.112532881837446 30.76393575608451 19.638556447364657 18-19 17.03458511991707 31.88387261798184 32.71972455758914 18.36181770451195 20-21 19.081057431807093 29.209786797247045 31.92202197654265 19.787133794403218 22-23 18.709962930742915 28.55577972481587 33.0949548455055 19.63930249893571 24-25 20.343785544585106 29.72301303666014 31.721081430330933 18.212119988423822 26-27 18.658699321339686 28.263938787633002 34.85719189780887 18.220169993218445 28-29 17.585877846536526 30.87546513134754 33.32503067216213 18.213626349953802 30-31 18.710733898479088 29.156842252302738 32.383794218279554 19.748629630938616 32-33 18.245112690847403 29.861223308290924 33.44087433018284 18.452789670678836 34-35 19.810992081190935 29.15165234660989 32.38043944761888 18.656916124580285 36-37 18.830580973887642 28.525538552663903 33.63678278516115 19.0070976882873 38-39 18.123904169288807 30.43876977543253 33.03914870990648 18.398177345372186 40-41 17.836735518000282 30.611922116555952 32.98436543030401 18.566976935139756 42-43 18.04980160800164 30.150535607914144 30.74217790327086 21.057484880813355 44-45 18.95514927520865 28.718836833167554 31.550008330935036 20.776005560688755 46-47 20.752284405785197 30.140186522637038 31.318365966098728 17.789163105479037 48-49 19.493180012657035 29.847375011781814 31.342823865242973 19.316621110318177 50-51 17.06933639445363 30.336061186498302 32.92662400398574 19.66797841506233 52-53 18.627092820646638 31.724846168738797 30.06893997424221 19.57912103637236 54-55 18.8824218513307 29.380398704647515 30.78195797510284 20.955221468918946 56-57 20.420312289675596 29.50380266523085 29.51086956521739 20.565015479876163 58-59 20.970740184343736 28.90620137172734 30.51479308853984 19.608265355389083 60-61 19.69363586203184 30.439595192915874 31.715215255628674 18.151553689423608 62-63 18.29020861372813 30.746971736204575 31.830753701211307 19.13206594885599 64-65 18.564947288206586 31.41747370298036 30.511776253702948 19.505802755110107 66-67 19.33667547061891 29.94364349040257 29.07188398970442 21.647797049274097 68-69 18.712306136834293 29.2101469145917 31.297030585757373 20.780516362816627 70-71 20.89328522041939 29.135565705249522 30.174535483274035 19.796613591057056 72-73 19.32046446368307 28.025457347334985 32.647718899203575 20.006359289778366 74-75 18.235975982139703 31.715150464929653 30.078349100171437 19.97052445275921 76-77 18.48230301439384 29.265567525181645 30.770913472055355 21.481215988369158 78-79 18.830364684463976 29.138244924468555 31.309290250066464 20.722100141001008 80-81 20.27007246462155 29.440119523722196 29.687613041949394 20.60219496970686 82-83 19.298652925055393 28.741468272995075 31.588377503999837 20.371501297949692 84-85 18.69874110250686 29.19488096579964 30.967734337804444 21.138643593889057 86-87 18.71760056129583 30.142056974218416 30.497073219156075 20.643269245329684 88-89 18.90594557488921 29.238438786051503 29.42166169211149 22.4339539469478 90-91 19.52250901521691 27.42443287849187 30.658300141517454 22.394757964773763 92-93 21.068004125438915 28.896074944352 30.62616827147138 19.409752658737702 94-95 19.629971718762356 27.84044738528938 31.211651668021002 21.31792922792726 96-97 18.193244859945178 28.429605773485918 32.23826903111712 21.13888033545178 98-99 19.39189666719392 29.29810154911707 29.723999367295225 21.586002416393786 100-101 19.062518925247794 27.495441118086816 30.925873589438197 22.516166367227193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 758.0 1 476.0 2 207.0 3 201.5 4 148.0 5 135.0 6 219.0 7 373.0 8 551.0 9 668.5 10 632.0 11 555.5 12 576.5 13 628.5 14 655.5 15 657.0 16 682.5 17 701.5 18 709.0 19 748.0 20 842.5 21 962.0 22 1084.5 23 1256.5 24 1462.0 25 1756.5 26 2296.0 27 3057.0 28 3343.0 29 3803.0 30 4916.0 31 5912.0 32 7603.0 33 9708.5 34 11039.5 35 12351.5 36 15304.5 37 17780.5 38 17070.5 39 15166.0 40 13438.5 41 11446.0 42 9177.5 43 8613.5 44 9101.0 45 8984.5 46 8631.5 47 12084.0 48 19977.5 49 18585.0 50 9534.0 51 4605.0 52 2883.0 53 2258.5 54 1876.5 55 1656.0 56 1425.0 57 1185.5 58 1062.5 59 976.0 60 865.5 61 698.0 62 541.0 63 392.5 64 263.0 65 203.0 66 147.0 67 116.5 68 95.0 69 72.5 70 61.0 71 51.5 72 41.0 73 32.5 74 29.0 75 28.5 76 27.0 77 16.0 78 10.0 79 7.5 80 3.5 81 1.0 82 1.5 83 1.5 84 0.5 85 1.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25988066978031016 2 0.22865469782996284 3 0.21555993539917204 4 0.22462554015895025 5 0.22563282957670344 6 0.2555157489700466 7 0.25685880152705076 8 0.24309251281775784 9 0.23805606572899216 10-11 0.2462822626406428 12-13 0.2494720124635277 14-15 0.23436267119723064 16-17 0.24930413089390221 18-19 0.23738453945049004 20-21 0.23301961864022644 22-23 0.2291583425388394 24-25 0.22395401388044817 26-27 0.2348663159061072 28-29 0.24695378891914488 30-31 0.247625315197647 32-33 0.24611438107101724 34-35 0.2523259991471616 36-37 0.23167656608322224 38-39 0.22831893469071177 40-41 0.22395401388044817 42-43 0.23167656608322224 44-45 0.24963989403315326 46-47 0.25534786740042104 48-49 0.2555157489700466 50-51 0.258537617223306 52-53 0.2791870502872454 54-55 0.25753032780555285 56-57 0.22462554015895025 58-59 0.20565492279126613 60-61 0.2017936466898791 62-63 0.21119501458890838 64-65 0.20129000198100253 66-67 0.2061585675001427 68-69 0.20666221220901926 70-71 0.20531915965201508 72-73 0.21018772517115525 74-75 0.21270594871553813 76-77 0.23100503980472017 78-79 0.22428977701969924 80-81 0.21807815894355487 82-83 0.21136289615853393 84-85 0.20968408046227868 86-87 0.22143579033606534 88-89 0.21824604051318036 90-91 0.23251597393134987 92-93 0.2177423958043038 94-95 0.2133774749940402 96-97 0.22747952684258416 98-99 0.23234809236172435 100-101 0.20380822552538538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 297829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.03170611323948 #Duplication Level Percentage of deduplicated Percentage of total 1 81.68086360771717 26.980582817657112 2 7.520990465347943 4.96862293463699 3 2.71198845270284 2.687448166565378 4 1.5125332899632031 1.9984622048222302 5 1.0276687877370956 1.6972826689140412 6 0.6739311634715078 1.335665767940664 7 0.510276687877371 1.1798716713281783 8 0.38524873447315455 1.0180338382091738 9 0.32832543861432434 0.9760634458027929 >10 2.9539124601028686 20.32642892397987 >50 0.42285876923702453 9.688109619949703 >100 0.2459899571042306 13.472160199309 >500 0.006098924556303239 1.4105409479936473 >1k 0.018296773668909715 10.407985790503947 >5k 0.0010164874260505398 1.852741002387276 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5518 1.852741002387276 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4010 1.3464101883966975 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2745 0.9216698172441233 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2408 0.8085176393165204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 0.7406934851878091 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1875 0.6295558860957127 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1770 0.5943007564743528 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1723 0.5785198889295535 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1615 0.5422574698904405 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1565 0.5254693129278881 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1564 0.5251335497886371 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1433 0.48114857854674997 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1406 0.47208297378697167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1335 0.4482437909001474 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1112 0.37336861084716394 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1087 0.36497453236588784 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1081 0.3629599535303815 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1058 0.35523740132760745 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1005 0.33744195494730195 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 864 0.29009935231290435 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 833 0.279690694996122 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 734 0.2464501442102683 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 609 0.20447975180388744 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 608 0.20414398866463643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 553 0.18567701600582884 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 472 0.15848020172649405 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 468 0.15713714916948987 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 424 0.14236357104244382 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 412 0.13833441337143126 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 400 0.13430525570041868 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 388 0.13027609802940615 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 374 0.12557541407989148 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 373 0.12523965094064043 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 352 0.11818862501636845 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 340 0.11415946734535588 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 334 0.11214488850984962 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 327 0.10979454653509228 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 326 0.10945878339584125 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 313 0.10509386258557762 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 305 0.10240775747156926 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.036447088765701E-4 0.0 22-23 0.0 0.0 0.0 0.0010072894177531402 0.0 24-25 0.0 0.0 0.0 0.002350341974757327 0.0 26-27 0.0 0.0 0.0 0.004029157671012561 0.0 28-29 0.0 0.0 0.0 0.008897723190152739 0.0 30-31 0.0 0.0 0.0 0.014773578127046057 0.0 32-33 0.0 0.0 0.0 0.022999775038696702 0.0 34-35 0.0 0.0 0.0 0.03240114293772601 0.0 36-37 0.0 0.0 0.0 0.040123695140500085 0.0 38-39 0.0 0.0 0.0 0.04818201048252521 0.0 40-41 0.0 0.0 0.0 0.06362711488807336 0.0 42-43 0.0 0.0 0.0 0.08478019266088929 0.0 44-45 0.0 0.0 0.0 0.12910092704202747 0.0 46-47 0.0 0.0 0.0 0.2263043558552055 0.0 48-49 0.0 0.0 0.0 0.2937927468446659 0.0 50-51 0.0 0.0 0.0 0.38461667601207405 0.0 52-53 0.0 0.0 0.0 0.49038206487615377 0.0 54-55 0.0 0.0 0.0 0.6095779793102754 0.0 56-57 0.0 0.0 0.0 0.7628538523783782 0.0 58-59 0.0 0.0 0.0 0.9547424864603513 0.0 60-61 0.0 0.0 0.0 1.177857092492672 0.0 62-63 0.0 0.0 0.0 1.4202780790319278 0.0 64-65 0.0 0.0 0.0 1.6598450788875496 0.0 66-67 0.0 0.0 0.0 1.997790678543728 0.0 68-69 0.0 0.0 0.0 2.4067501821515034 0.0 70-71 0.0 0.0 0.0 2.7275718617058784 0.0 72-73 0.0 0.0 0.0 3.1691003898210046 0.0 74-75 0.0 0.0 0.0 3.607942812822123 0.0 76-77 0.0 0.0 0.0 4.002632383011728 0.0 78-79 0.0 0.0 0.0 4.545729260750296 0.0 80-81 0.0 0.0 0.0 5.036950733474578 0.0 82-83 0.0 0.0 0.0 5.595996360327571 0.0 84-85 0.0 0.0 0.0 6.284310795792216 0.0 86-87 0.0 0.0 0.0 7.088463514298473 0.0 88-89 0.0 0.0 0.0 8.089037669266592 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACC 35 2.776093E-4 54.31922 4 CTTTGCG 70 7.856215E-8 47.529316 2 TTGCGTT 80 2.2441236E-7 41.588154 4 GCTTTGC 85 3.6110396E-7 39.141792 1 TACCTCC 50 0.0016116666 38.010643 6 TTTGCGT 110 2.7113765E-6 30.24593 3 ATATAGG 130 3.0077717E-7 29.24881 2 ACTTGCT 135 4.2032298E-7 28.15129 8 CACTACC 70 0.008356528 27.15961 3 GCTACAC 70 0.008356528 27.15961 3 CATGGGG 290 0.0 24.58413 4 TCCTACA 120 1.699528E-4 23.76466 2 AACTTGC 160 1.8500286E-6 23.752653 7 CTCGGTG 60 1.7101174E-4 23.748653 94-95 GAGTACT 1780 0.0 22.948137 12-13 CTAGTAG 125 2.2461695E-4 22.806387 6 CTTGCTA 170 3.1375093E-6 22.351673 9 GTACTTT 1825 0.0 22.252163 14-15 CAATACT 235 4.5820343E-9 22.247766 4 CTAATAC 130 2.9221788E-4 21.936607 3 >>END_MODULE