##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 297829 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07740347649155 31.0 31.0 34.0 30.0 34.0 2 32.29400427762239 34.0 31.0 34.0 30.0 34.0 3 32.32577754348972 34.0 31.0 34.0 30.0 34.0 4 35.99346604931017 37.0 35.0 37.0 35.0 37.0 5 35.90750732803051 37.0 35.0 37.0 35.0 37.0 6 35.97042262506338 37.0 35.0 37.0 35.0 37.0 7 35.91949742973317 37.0 35.0 37.0 35.0 37.0 8 35.91141225334 37.0 35.0 37.0 35.0 37.0 9 37.570562302529304 39.0 37.0 39.0 35.0 39.0 10-11 37.49209109925494 39.0 37.0 39.0 34.5 39.0 12-13 37.443707630888866 39.0 37.0 39.0 34.0 39.0 14-15 38.51062018809451 40.0 38.0 41.0 34.0 41.0 16-17 38.53919195242908 40.0 38.0 41.0 34.0 41.0 18-19 38.621133267747595 40.0 38.0 41.0 34.0 41.0 20-21 38.55010761208613 40.0 38.0 41.0 34.0 41.0 22-23 38.41264786169245 40.0 38.0 41.0 34.0 41.0 24-25 38.3255609762649 40.0 38.0 41.0 34.0 41.0 26-27 38.229426953050236 40.0 37.5 41.0 34.0 41.0 28-29 38.13972447276793 40.0 37.5 41.0 34.0 41.0 30-31 38.194584812090156 40.0 37.5 41.0 34.0 41.0 32-33 38.039529394384026 40.0 37.0 41.0 33.5 41.0 34-35 37.859367287940394 40.0 37.0 41.0 33.0 41.0 36-37 37.66896104811822 40.0 36.5 41.0 33.0 41.0 38-39 37.50675555436173 40.0 36.0 41.0 33.0 41.0 40-41 37.40051170302422 40.0 36.0 41.0 33.0 41.0 42-43 37.131283387447155 40.0 36.0 41.0 32.5 41.0 44-45 36.81137162600015 40.0 36.0 41.0 31.0 41.0 46-47 36.37787623099161 40.0 36.0 41.0 30.5 41.0 48-49 35.97347974844626 39.0 35.0 41.0 29.5 41.0 50-51 35.703427134362336 39.0 35.0 41.0 28.0 41.0 52-53 35.53733182463763 39.0 35.0 41.0 27.5 41.0 54-55 35.35877970244671 39.0 35.0 40.5 27.0 41.0 56-57 35.053686847150544 39.0 35.0 40.0 26.0 41.0 58-59 34.81193067162701 38.5 35.0 40.0 26.0 41.0 60-61 34.44356157392329 38.0 34.0 40.0 23.5 41.0 62-63 34.11967605572325 38.0 34.0 40.0 22.5 41.0 64-65 33.74801480043918 37.5 33.5 40.0 22.0 41.0 66-67 33.26786679604739 37.0 33.0 39.0 20.0 41.0 68-69 32.79043847308355 36.0 32.5 39.0 18.5 40.0 70-71 32.293136329907426 36.0 32.0 39.0 15.0 40.0 72-73 31.800580198704623 35.0 31.5 38.0 10.5 40.0 74-75 31.361281137834126 35.0 31.0 37.0 7.5 39.0 76-77 30.026236531701077 33.5 29.5 36.0 7.0 38.5 78-79 30.604872258913673 34.0 31.0 36.0 2.0 39.0 80-81 30.351579261925465 34.0 31.0 36.0 2.0 38.0 82-83 29.9937245869274 34.0 30.5 35.5 2.0 37.0 84-85 29.60049390757784 34.0 30.0 35.0 2.0 37.0 86-87 29.318850749926973 34.0 30.0 35.0 2.0 36.0 88-89 28.808081147235495 34.0 29.0 35.0 2.0 36.0 90-91 28.33432271538366 34.0 29.0 35.0 2.0 35.5 92-93 28.032730190814192 34.0 29.0 35.0 2.0 35.0 94-95 27.63093923022943 34.0 27.0 35.0 2.0 35.0 96-97 27.19007887076141 33.0 27.0 35.0 2.0 35.0 98-99 26.566128550275494 33.0 25.5 35.0 2.0 35.0 100-101 24.209014232999472 31.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -3.092735586562867E-5 1101 2 0.1240578921715354 1101 3 0.2370731863621245 1101 4 0.18148523177539033 1101 5 0.0722782553321153 1101 6 -0.10077850514818465 1101 7 0.020175311832531406 1101 8 -0.0028356746687521195 1101 9 0.0695621681467884 1101 10-11 0.02364246626406441 1101 12-13 -0.30828899114992225 1101 14-15 -0.2191346171239843 1101 16-17 -0.015682489385135057 1101 18-19 -0.04210385161338337 1101 20-21 0.1294728434831569 1101 22-23 0.3376363002755909 1101 24-25 -0.03584384156319942 1101 26-27 0.4365205352531092 1101 28-29 -0.06014616007279017 1101 30-31 0.049689361802961685 1101 32-33 -0.05515922281163199 1101 34-35 0.04291604000565741 1101 36-37 -0.14915676240620002 1101 38-39 -0.09523786646548871 1101 40-41 0.29470746703717765 1101 42-43 0.24717307877535433 1101 44-45 0.6143594330292075 1101 46-47 0.07576187856545147 1101 48-49 0.3753254825522063 1101 50-51 0.48167707968481466 1101 52-53 0.4807932245781501 1101 54-55 0.21721870892952921 1101 56-57 0.4767448313120042 1101 58-59 0.23911133790259953 1101 60-61 0.5980123413934919 1101 62-63 0.8277786153640392 1101 64-65 0.9062627454882772 1101 66-67 0.7628882770053735 1101 68-69 0.6509607112895281 1101 70-71 0.8890678879055756 1101 72-73 0.726991369885436 1101 74-75 0.8477593384129349 1101 76-77 0.756275470266818 1101 78-79 0.7626620669314477 1101 80-81 0.7291549573427147 1101 82-83 0.7311352609281236 1101 84-85 0.8000665350378746 1101 86-87 1.2279825136104385 1101 88-89 0.8099156703404624 1101 90-91 0.8773967933658007 1101 92-93 1.001379329401523 1101 94-95 1.2385610693709275 1101 96-97 1.295692095997385 1101 98-99 0.6789031474035703 1101 100-101 1.3507428015365122 1102 1 -0.10132962865456818 1102 2 -0.007976739863096327 1102 3 0.09183509112403243 1102 4 0.057459257749414405 1102 5 -0.012570229516370546 1102 6 0.006797252427574563 1102 7 -0.18047403881681845 1102 8 -0.08443740627048868 1102 9 0.00960545819007308 1102 10-11 -0.032418139796533296 1102 12-13 0.09106165820072931 1102 14-15 -0.07151556950493898 1102 16-17 -0.07498984869248915 1102 18-19 -0.09838090789043719 1102 20-21 0.025901414911722043 1102 22-23 -0.046887509248215054 1102 24-25 0.012532781813426652 1102 26-27 0.09799239672497606 1102 28-29 -0.21057906050569386 1102 30-31 0.15639931851291777 1102 32-33 0.3555550629026527 1102 34-35 0.5493013213909421 1102 36-37 0.18958782633838922 1102 38-39 0.15779243656481157 1102 40-41 0.6225213198510318 1102 42-43 0.7098354164376914 1102 44-45 0.3354633291331055 1102 46-47 0.5697012725048438 1102 48-49 0.8897194219461468 1102 50-51 0.7933653913731291 1102 52-53 0.9594728782578059 1102 54-55 0.9022836439944655 1102 56-57 0.9636495932167648 1102 58-59 0.6682238920151491 1102 60-61 0.58946255784371 1102 62-63 0.7616530742384953 1102 64-65 0.758968373193909 1102 66-67 1.00747702159412 1102 68-69 1.0397053000341216 1102 70-71 0.8207778446155345 1102 72-73 0.7313203742144445 1102 74-75 0.3001402907938875 1102 76-77 0.3640676780590262 1102 78-79 0.4469694262388053 1102 80-81 0.17125452444227918 1102 82-83 0.5426071223999855 1102 84-85 0.6235513835227273 1102 86-87 0.5986535092814336 1102 88-89 0.6349156703404653 1102 90-91 0.6338902998593063 1102 92-93 0.7728079008300917 1102 94-95 0.7224355282453843 1102 96-97 0.3695016198069112 1102 98-99 0.9765221950226177 1102 100-101 1.1786648794585908 1103 1 0.1775051588489518 1103 2 0.04193413357718612 1103 3 0.24693406851402955 1103 4 0.12984878772408592 1103 5 0.07537423056431436 1103 6 0.24196002567084207 1103 7 0.21997628485331688 1103 8 0.19756841041025552 1103 9 0.056926970527065635 1103 10-11 0.16598696387173106 1103 12-13 0.193158477789801 1103 14-15 0.08901543715609961 1103 16-17 0.3014338308271647 1103 18-19 0.30540931633321833 1103 20-21 0.008634316684876353 1103 22-23 0.08624411660787246 1103 24-25 0.3104781800684222 1103 26-27 0.12935556400145742 1103 28-29 0.21291282428650504 1103 30-31 0.07976454977288938 1103 32-33 -0.015891601887666695 1103 34-35 0.07881628527123041 1103 36-37 -0.04955280599109102 1103 38-39 -0.17517031791496862 1103 40-41 -0.11330286628275132 1103 42-43 0.13412474939575958 1103 44-45 0.20617821795944735 1103 46-47 0.32498788475739815 1103 48-49 0.12726046707233252 1103 50-51 -0.012080710512606174 1103 52-53 -0.16943696319074064 1103 54-55 -0.013843572442347352 1103 56-57 0.07358955006075263 1103 58-59 0.327009894454406 1103 60-61 0.1603087910738381 1103 62-63 0.42321607264359784 1103 64-65 0.23772810675239953 1103 66-67 0.6095972955410218 1103 68-69 0.45250668853210385 1103 70-71 0.48278647581921064 1103 72-73 0.4874654159631966 1103 74-75 0.3693306463619521 1103 76-77 0.44971361107338126 1103 78-79 0.4694340101584977 1103 80-81 0.53958175964258 1103 82-83 0.27388585398831466 1103 84-85 0.17021148296920074 1103 86-87 0.5274226245830569 1103 88-89 0.6932185130086346 1103 90-91 0.8166232016324564 1103 92-93 0.5680935749083353 1103 94-95 0.3964809358821242 1103 96-97 0.33050171362434 1103 98-99 0.5862651352609163 1103 100-101 1.1303254479922273 1104 1 -0.24897475461294505 1104 2 -0.030886560965690535 1104 3 -0.09456505491466771 1104 4 -0.12058017269965404 1104 5 0.084326448103198 1104 6 -0.07449153910217632 1104 7 -0.08567666751342529 1104 8 -0.33666449644938723 1104 9 0.14748424606886346 1104 10-11 -0.12586465312256223 1104 12-13 -0.17139726841482883 1104 14-15 0.011443539658991142 1104 16-17 -0.2889182897293665 1104 18-19 -0.3442279323519202 1104 20-21 -0.2881456825691018 1104 22-23 -0.28139827960235664 1104 24-25 -0.14042450186675381 1104 26-27 -0.37565674529766113 1104 28-29 -0.3459386808050766 1104 30-31 -0.23602492391132301 1104 32-33 -0.26752817915649274 1104 34-35 -0.15963442158094665 1104 36-37 -0.15164072422750507 1104 38-39 -0.30580741739648687 1104 40-41 -0.5389062084439615 1104 42-43 -0.2607286580420478 1104 44-45 -0.0074720487420378845 1104 46-47 -0.0929128202297278 1104 48-49 -0.09907210780924203 1104 50-51 -0.18390733589960462 1104 52-53 0.2050734624126065 1104 54-55 0.397902482986801 1104 56-57 0.33562841760522844 1104 58-59 0.47563535605311813 1104 60-61 0.7763099474019413 1104 62-63 0.9088692115148689 1104 64-65 0.6538570186849597 1104 66-67 0.5378256889909778 1104 68-69 0.4568009553827821 1104 70-71 0.3174828464410169 1104 72-73 0.13365699341852633 1104 74-75 0.010253470786587116 1104 76-77 0.509796046076552 1104 78-79 0.3553118869909042 1104 80-81 0.3268313773082916 1104 82-83 0.41936871422182165 1104 84-85 0.37865361061290415 1104 86-87 0.6507254333413108 1104 88-89 0.6174184085660919 1104 90-91 0.893337178282092 1104 92-93 0.8841441549374345 1104 94-95 1.109082114590766 1104 96-97 1.1296969465685045 1104 98-99 1.4539774706706652 1104 100-101 1.863358198188056 1105 1 0.0422740730329636 1105 2 -0.21519865965986895 1105 3 0.056596477442305115 1105 4 -0.06594847224513956 1105 5 -0.03550617580560811 1105 6 -0.0260642972004419 1105 7 -0.12375112101605623 1105 8 0.057841671936735395 1105 9 0.053172868823352815 1105 10-11 -0.1074136034147557 1105 12-13 -0.09238962105893478 1105 14-15 -0.16557290937065972 1105 16-17 -2.847135083143826E-4 1105 18-19 -0.11856780681985413 1105 20-21 -0.189749102235794 1105 22-23 -0.016094549712740047 1105 24-25 0.12312069689236438 1105 26-27 -0.09363344250564865 1105 28-29 -0.09644369487331517 1105 30-31 -0.12973749876162088 1105 32-33 -0.12716132250834278 1105 34-35 -0.03672662272083471 1105 36-37 -0.4618365975411223 1105 38-39 -0.46445397969358027 1105 40-41 -0.7223525687354311 1105 42-43 -0.6268548394142357 1105 44-45 -0.500492032870163 1105 46-47 -0.2715435106561088 1105 48-49 -0.3290158348130632 1105 50-51 -0.3321109287139734 1105 52-53 -0.5328061999494196 1105 54-55 -0.6064262090267647 1105 56-57 -0.3342727439271229 1105 58-59 -0.1622515207955928 1105 60-61 -0.5555474221962129 1105 62-63 0.025879167506502654 1105 64-65 -0.24939671481190828 1105 66-67 -0.13727892106601303 1105 68-69 -0.36014046298529223 1105 70-71 -0.36990442720446026 1105 72-73 0.04764227586568026 1105 74-75 0.25677248095901106 1105 76-77 0.5609647929202133 1105 78-79 0.3605604260573507 1105 80-81 -0.21705975608756845 1105 82-83 -0.23682492260092047 1105 84-85 0.013934254145116398 1105 86-87 0.12432943016693443 1105 88-89 0.06569955711237441 1105 90-91 0.550874513250708 1105 92-93 0.07947638204947438 1105 94-95 0.00756526876434549 1105 96-97 -0.05184659285866644 1105 98-99 0.6278105274486734 1105 100-101 1.300330638848905 1106 1 -0.2558750832000207 1106 2 -0.044340376226735145 1106 3 -0.06574066645173104 1106 4 0.04836834865850648 1106 5 0.05409643715029944 1106 6 -0.0022936566633333655 1106 7 -0.03501949336227739 1106 8 -0.03898286081594193 1106 9 0.200514549099168 1106 10-11 0.0509151935367953 1106 12-13 0.20621317335224632 1106 14-15 0.00727230928294631 1106 16-17 0.0341010603984202 1106 18-19 -0.09232030182983664 1106 20-21 -0.08925010023978786 1106 22-23 -0.1120390243997349 1106 24-25 -0.13746721818657193 1106 26-27 -0.09746214872956926 1106 28-29 -0.3863366362632661 1106 30-31 -0.16935825724466014 1106 32-33 0.0934338507814445 1106 34-35 0.008392230481845786 1106 36-37 0.06534540209596429 1106 38-39 -0.03917726040487679 1106 40-41 0.08918798651770032 1106 42-43 -0.025013068410792982 1106 44-45 0.262736056405835 1106 46-47 0.013640666444239002 1106 48-49 0.1579012401279627 1106 50-51 0.1418502398579733 1106 52-53 0.13068499946992773 1106 54-55 0.19470788641870485 1106 56-57 0.030316259883427676 1106 58-59 0.062163285954540015 1106 60-61 0.4682504366315854 1106 62-63 0.4965015590869797 1106 64-65 0.23927140349692877 1106 66-67 0.2726285367456356 1106 68-69 0.24576590609472504 1106 70-71 0.33441420825189994 1106 72-73 0.4934415863356527 1106 74-75 0.5713524120060072 1106 76-77 0.5852797992711487 1106 78-79 0.5590906383600185 1106 80-81 0.34852725171500865 1106 82-83 0.47291015270301884 1106 84-85 0.6387028986742393 1106 86-87 0.8138050244329484 1106 88-89 0.7364308218556168 1106 90-91 0.6308599968290061 1106 92-93 0.6409897190119125 1106 94-95 0.49061734642720367 1106 96-97 0.7391985895038786 1106 98-99 0.4250070435074669 1106 100-101 1.0680588188525313 1107 1 0.04610120138495688 1107 2 -0.06463023129919776 1107 3 0.08419345739543616 1107 4 -0.11592677650618555 1107 5 -0.061055078001217566 1107 6 -0.09728706904805051 1107 7 -0.1209219966560795 1107 8 -0.17442423103992155 1107 9 0.03516540548915259 1107 10-11 -0.04598862728006736 1107 12-13 0.028676941468184225 1107 14-15 0.027957421272397198 1107 16-17 -0.11754584342239838 1107 18-19 -0.03579856269939796 1107 20-21 -0.03490227415282732 1107 22-23 0.07504924964506188 1107 24-25 -0.05228935257392919 1107 26-27 -0.09805503410505878 1107 28-29 -0.41505863889831573 1107 30-31 -0.027329271737407623 1107 32-33 0.12083833035983815 1107 34-35 0.05799697356485467 1107 36-37 -0.11159794441260118 1107 38-39 -0.03673984275007314 1107 40-41 -0.07082518871287391 1107 42-43 0.15634398033756014 1107 44-45 0.1321036453386384 1107 46-47 -0.3285859475215105 1107 48-49 -0.42431219860721825 1107 50-51 -0.5694145822764156 1107 52-53 -0.1802504419002986 1107 54-55 -0.14672821371303968 1107 56-57 0.10271415184654842 1107 58-59 -0.002922353044141346 1107 60-61 -0.26488526824324765 1107 62-63 -0.005540601650835697 1107 64-65 -0.03299473616051074 1107 66-67 0.06347175150189344 1107 68-69 0.4065695951592829 1107 70-71 0.33467771286322545 1107 72-73 0.2416629302091664 1107 74-75 0.22675425653828896 1107 76-77 0.19410720375072543 1107 78-79 -0.03267484253589714 1107 80-81 -0.06892992878564996 1107 82-83 -0.010620809088816685 1107 84-85 0.17052108049242065 1107 86-87 0.2947668162643069 1107 88-89 0.29123978101240056 1107 90-91 0.3643909586208345 1107 92-93 0.39448115511204307 1107 94-95 -0.07828910943577583 1107 96-97 0.1438099202021661 1107 98-99 0.7652573728882288 1107 100-101 1.3276108610132695 1108 1 -0.3398323024513594 1108 2 -0.3404188075992849 1108 3 -0.5094126807119785 1108 4 -0.05885090267839388 1108 5 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-1.0409528871442753 2316 84-85 -1.0649119903737194 2316 86-87 -1.2180370180390163 2316 88-89 -1.0727793905042056 2316 90-91 -1.2428097717226514 2316 92-93 -0.9678148622336131 2316 94-95 -1.3265146029882082 2316 96-97 -0.9794127204460104 2316 98-99 -1.0473981103937504 2316 100-101 -2.0520079909780584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 3.0 5 8.0 6 14.0 7 34.0 8 91.0 9 192.0 10 293.0 11 553.0 12 815.0 13 1209.0 14 1495.0 15 2137.0 16 2946.0 17 4240.0 18 3680.0 19 2070.0 20 1818.0 21 1929.0 22 1857.0 23 2074.0 24 2197.0 25 2539.0 26 2782.0 27 3403.0 28 4218.0 29 5178.0 30 6427.0 31 8068.0 32 10047.0 33 13102.0 34 17736.0 35 25427.0 36 38992.0 37 63197.0 38 60514.0 39 6542.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.78077294004187 23.752734457695293 17.191044083994473 19.275448518268362 2 14.271947997004993 25.329299698820464 37.43691850021321 22.961833803961333 3 19.38360602896293 27.93582894882634 33.05218766473379 19.628377357476943 4 11.005979941510061 20.235437113242835 41.957633407089304 26.800949538157802 5 10.078771354894165 41.53258300204147 36.383904587944556 12.004741055119803 6 25.53814437143462 41.794788284552546 17.067176131269957 15.599891212742884 7 22.54011530106202 36.39907463678823 24.56812466213834 16.492685400011418 8 23.516514510004065 33.076362610759865 22.094221852136627 21.312901027099443 9 22.760375920410706 19.25937366744004 25.544188107941135 32.43606230420812 10-11 22.301798353412035 28.347401856104863 29.043609062949084 20.307190727534014 12-13 25.054103014826488 27.05553680767868 29.01936291916195 18.87099725833287 14-15 21.57479627571526 29.538258530901963 29.63462255186701 19.252322641515768 16-17 17.508536777815458 32.02072330095457 30.809625657676047 19.66111426355392 18-19 17.157496415728488 31.8216157593787 32.77115391718066 18.249733907712145 20-21 19.061273415281924 29.049723163291684 32.13353971574293 19.75546370568346 22-23 18.712436120419305 28.57406673695379 33.11178339030172 19.601713752325185 24-25 20.52570875996595 29.6311778837679 31.71729177893841 18.125821577327738 26-27 18.862324205535774 28.12593909541293 34.83101738764012 18.180719311411174 28-29 17.771976361560675 30.852864146128532 33.35471089920086 18.020448593109933 30-31 18.8788812315854 28.916570833788015 32.55323971090648 19.651308223720108 32-33 18.439943726097862 29.736526664629704 33.568423491332275 18.25510611794016 34-35 19.993519771412455 28.991636140201255 32.43371196223336 18.581132126152927 36-37 18.861494347427552 28.426714658411374 33.82192466146681 18.889866332694265 38-39 18.098624534754297 30.45699425672329 33.04104906715823 18.403332141364185 40-41 17.911482614994874 30.616277488554655 33.04183447856736 18.43040541788311 42-43 17.968104612036832 30.11776913622534 30.97140228217579 20.942723969562035 44-45 19.143063877365666 28.593636942065654 31.596707501129007 20.666591679439676 46-47 20.81949709397003 30.231441531885743 31.32670089212266 17.622360482021563 48-49 19.657420869022157 29.772117557390317 31.36413848214915 19.206323091438378 50-51 17.23606499031323 30.34459370981335 32.94155371035057 19.477787589522848 52-53 18.946106658518815 31.638624848486884 29.911123497040247 19.504144995954054 54-55 18.97330347279815 29.481682441938162 30.72820309640767 20.816810988856023 56-57 20.422625063375293 29.552024819611255 29.578717989181712 20.446632127831744 58-59 21.103552709776416 28.93892132733884 30.426519915790607 19.531006047094138 60-61 19.73044935180926 30.665079626228476 31.509523921444853 18.094947100517413 62-63 18.341397244727677 30.62495593108797 31.85636724429119 19.17727957989316 64-65 18.551249206759586 31.69352212175443 30.353491433003505 19.401737238482486 66-67 19.33894953144254 30.184098929251345 28.90584865812261 21.5711028811835 68-69 18.558803877392734 29.529696570851062 30.935033190186317 20.976466361569894 70-71 20.77148296505713 29.499477888318467 29.798810726960774 19.93022841966363 72-73 19.2617240094148 28.201921236682793 32.39190945139661 20.1444453025058 74-75 18.273740972168593 31.939300739686193 29.592987922599885 20.19397036554533 76-77 18.434907279009096 29.42124507687297 30.70906459747036 21.434783046647574 78-79 18.684379291472624 29.288282873729553 31.11567375910338 20.911664075694443 80-81 20.173153050911765 29.630593394195998 29.47295260031763 20.723300954574604 82-83 19.040719879796193 28.94830061025258 31.440515063249702 20.570464446701532 84-85 18.499939562432008 29.412613991968517 30.67878104140645 21.408665404193027 86-87 18.04055347195874 30.34459370981335 30.145318286667855 21.469534531560054 88-89 18.31285737789134 29.53943370188934 29.23707899499377 22.91062992522555 90-91 18.917180983593347 27.61378212661934 30.417117183669905 23.051919706117406 92-93 20.163453886730622 29.08156241500767 30.343394186440847 20.41158951182086 94-95 18.819020310312293 27.947412777130502 30.914887401831255 22.318679510725953 96-97 17.009592752888402 28.716478247585027 31.98949732900423 22.28443167052235 98-99 18.23065662059981 29.400694360503376 29.341431967444297 23.027217051452517 100-101 17.35619890630745 27.95799844525745 30.572182197022485 24.113620451412615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 467.0 1 300.0 2 155.5 3 172.0 4 134.0 5 122.5 6 215.0 7 359.5 8 512.0 9 620.0 10 585.0 11 525.0 12 559.0 13 611.0 14 624.0 15 630.5 16 686.5 17 704.5 18 720.5 19 788.5 20 880.5 21 965.5 22 1066.5 23 1237.5 24 1397.0 25 1709.0 26 2368.0 27 3112.5 28 3314.0 29 3780.0 30 4817.0 31 5760.5 32 7338.5 33 9308.0 34 10844.5 35 12539.0 36 15537.5 37 17864.5 38 17139.0 39 15063.5 40 13294.0 41 11370.5 42 9124.0 43 8685.0 44 9290.5 45 9207.5 46 8544.0 47 12168.0 48 20839.0 49 19085.5 50 9409.5 51 4652.5 52 2949.5 53 2208.5 54 1846.5 55 1617.5 56 1409.0 57 1249.5 58 1112.5 59 944.5 60 816.5 61 686.0 62 519.5 63 367.5 64 251.0 65 192.5 66 135.0 67 107.5 68 88.5 69 69.0 70 52.5 71 46.0 72 42.0 73 36.5 74 29.5 75 25.5 76 20.5 77 12.0 78 6.5 79 6.0 80 5.5 81 3.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07991162714174913 2 0.0 3 0.0 4 0.0 5 0.0016788156962552338 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.357631392510468E-4 12-13 0.005875854936893318 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010072894177531402 24-25 8.394078481276169E-4 26-27 8.394078481276169E-4 28-29 0.0030218682532594204 30-31 5.036447088765701E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.394078481276169E-4 40-41 1.678815696255234E-4 42-43 0.014437814987795011 44-45 1.678815696255234E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.036447088765701E-4 84-85 3.357631392510468E-4 86-87 0.0 88-89 0.0 90-91 0.007722552202774076 92-93 0.002686105114008374 94-95 0.0 96-97 0.0 98-99 3.357631392510468E-4 100-101 0.010240775747156925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 297829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.81322168089743 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64992770372247 25.657340285868734 2 7.999029013498824 5.089497664767366 3 2.893961941550834 2.7619875834791108 4 1.5778530644122892 2.0078635727212597 5 0.9804852821665664 1.5596197818211122 6 0.7609578992918131 1.4525113404000283 7 0.5815364805961013 1.2950384280912874 8 0.4126692630001372 1.0502670995772743 9 0.352510316731575 1.0093039965886463 >10 3.070217099916622 20.420106839830908 >50 0.43483308530960746 9.811670455194088 >100 0.25963334705379476 13.94390740995672 >500 0.007387940769823428 1.7328735616746522 >1k 0.017942141869571184 10.261928824929742 >5k 0.0010554201099747754 1.9460831550990667 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5796 1.9460831550990667 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3964 1.330965083991149 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2828 0.9495381578019602 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.8256415594183238 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2284 0.7668830100493907 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2014 0.6762269624516082 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1884 0.632577754348972 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1748 0.5869139674108297 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1542 0.517746760725114 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1538 0.5164037081681099 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1514 0.5083453928260847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1507 0.5059950508513275 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1442 0.4841704468000094 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1430 0.48014128912899684 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1179 0.3958647411769841 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1095 0.3676606374798962 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1074 0.3606096115556242 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1061 0.35624469074536064 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 970 0.32569024507351535 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 900 0.3021868253259421 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 785 0.2635740643120717 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 771 0.258873380362557 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 648 0.2175745142346783 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 577 0.19373533134785398 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.17123920101803383 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 500 0.16788156962552336 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 485 0.16284512253675767 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 456 0.1531079914984773 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 422 0.1416920447639417 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 396 0.1329622031434145 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 386 0.12960457175090404 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 382 0.12826151919389986 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 374 0.12557541407989148 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 369 0.12389659838363624 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 356 0.11953167757337264 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 350 0.11751709873786635 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 347 0.11650980932011322 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 324 0.10878725711733915 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 312 0.10475809944632658 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 310 0.10408657316782449 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 308 0.10341504688932239 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 301 0.10106470491456505 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.036447088765701E-4 0.0 22-23 0.0 0.0 0.0 0.0010072894177531402 0.0 24-25 0.0 0.0 0.0 0.002350341974757327 0.0 26-27 0.0 0.0 0.0 0.004029157671012561 0.0 28-29 0.0 0.0 0.0 0.009233486329403785 0.0 30-31 0.0 0.0 0.0 0.015109341266297104 0.0 32-33 0.0 0.0 0.0 0.02333553817794775 0.0 34-35 0.0 0.0 0.0 0.03256902450735154 0.0 36-37 0.0 0.0 0.0 0.04045945827975113 0.0 38-39 0.0 0.0 0.0 0.04868565519140178 0.0 40-41 1.6788156962552336E-4 0.0 0.0 0.06429864116657545 0.0 42-43 3.357631392510467E-4 0.0 0.0 0.08511595580014035 0.0 44-45 3.357631392510467E-4 0.0 0.0 0.12910092704202747 0.0 46-47 3.357631392510467E-4 0.0 0.0 0.22865469782996284 0.0 48-49 3.357631392510467E-4 0.0 0.0 0.297989786085304 0.0 50-51 3.357631392510467E-4 0.0 0.0 0.38982100467046527 0.0 52-53 3.357631392510467E-4 0.0 0.0 0.49676156452192366 0.0 54-55 3.357631392510467E-4 0.0 0.0 0.6176362946523005 0.0 56-57 3.357631392510467E-4 0.0 0.0 0.7699048783026501 0.0 58-59 3.357631392510467E-4 0.0 0.0 0.9609541045364958 0.0 60-61 3.357631392510467E-4 0.0 0.0 1.18692269725245 0.0 62-63 3.357631392510467E-4 0.0 0.0 1.4316940257664634 0.0 64-65 3.357631392510467E-4 0.0 0.0 1.6721004334702128 0.0 66-67 3.357631392510467E-4 0.0 0.0 2.0132357829492764 0.0 68-69 3.357631392510467E-4 0.0 0.0 2.42337045754443 0.0 70-71 3.357631392510467E-4 0.0 0.0 2.7470461237824386 0.0 72-73 3.357631392510467E-4 0.0 0.0 3.194282625264833 0.0 74-75 3.357631392510467E-4 0.0 0.0 3.636818442797713 0.0 76-77 3.357631392510467E-4 0.0 0.0 4.032347420835446 0.0 78-79 3.357631392510467E-4 0.0 0.0 4.580144982523528 0.0 80-81 3.357631392510467E-4 0.0 0.0 5.080599941577214 0.0 82-83 3.357631392510467E-4 0.0 0.0 5.648879054759611 0.0 84-85 3.357631392510467E-4 0.0 0.0 6.345419687135907 0.0 86-87 3.357631392510467E-4 0.0 0.0 7.158805891971568 0.0 88-89 3.357631392510467E-4 0.0 0.0 8.175328796054112 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTCAT 25 0.005016229 57.004032 7 GTCTTCG 40 5.366483E-4 47.5273 1 CTGTGTA 50 0.0016133331 38.002686 9 GTATAGT 140 4.4929038E-10 33.94807 1 TGTACAG 60 0.0039481726 31.66359 5 TGGGACA 85 6.399587E-4 27.938461 4 CCCTGGC 105 6.885631E-5 27.144777 7 GGTCGAA 50 0.0016520335 23.751678 90-91 ATGGGTA 120 1.7077985E-4 23.747692 5 CATGGGT 140 1.7750794E-5 23.74769 4 CATGGGC 245 2.9467628E-10 23.263044 4 GCCCTGG 125 2.2519383E-4 22.797785 6 GTACATG 1140 0.0 22.512932 1 GAGTACT 1890 0.0 22.494976 12-13 CCTGGCT 150 3.0218354E-5 22.168234 8 GTGAACG 65 2.9377997E-4 21.924627 86-87 CGATTGC 65 2.9394685E-4 21.922787 92-93 GTAGGGG 130 2.936359E-4 21.920946 6 ATGGGGG 195 4.2037027E-7 21.920944 5 AGTGTAC 110 0.0028628507 21.60332 1 >>END_MODULE