FastQCFastQC Report
Thu 26 May 2016
SRR937507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785124
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.24875014541542853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54770.1966519264492353No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47150.16929228285706488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.12372878191419844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90400.039.2824131
GGTATCA54200.038.5921861
ATCAACG113550.030.9622443
TCAACGC114550.030.7350854
TATCAAC116100.030.283832
CAACGCA116500.030.1401525
AACGCAG121400.028.9158136
ACGCAGA140550.024.8083747
CGCAGAG139700.024.6872848
TACATGG82950.022.0523872
GCAGAGT151700.021.763689
GTACATG86950.021.4866621
AGAGTAC124850.021.30654510-11
GAGTACT84050.020.93844812-13
AGTACTT88850.020.4220812-13
ACATGGG86000.019.943283
GTACTTT89050.019.54802114-15
TAAGGTG13150.018.427715
CAGAGTA146150.017.8437910-11
TACTTTT100150.017.8082714-15
Produced by FastQC (version 0.11.5)