FastQCFastQC Report
Thu 26 May 2016
SRR937507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785124
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77250.2773664655505464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.2333468815033011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.150442134712853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64550.038.8734321
GTATCAA100500.035.2294121
ATCAACG124850.028.0763873
TCAACGC125350.027.9643964
CAACGCA127600.027.5085185
TATCAAC128400.027.3741172
AACGCAG131450.026.7033086
CGCAGAG151150.023.0381288
ACGCAGA152200.023.0040457
GAGTACT93800.021.44855712-13
GTACATG87950.021.019821
TACATGG86400.020.8352582
AGAGTAC135550.020.60768510-11
GCAGAGT168750.020.353829
AGTACTT98100.020.31470312-13
GTACTTT99650.019.97147414-15
ACATGGG87450.019.8790073
CAGAGTA160750.018.07161110-11
TAAGGTG13600.017.8116725
TACTTTT111050.017.66464614-15
Produced by FastQC (version 0.11.5)