##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937507_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2785124 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.085095672580465 31.0 31.0 34.0 30.0 34.0 2 32.29917123977245 34.0 31.0 34.0 31.0 34.0 3 32.36989879086173 34.0 31.0 34.0 30.0 34.0 4 36.026056290491915 37.0 35.0 37.0 35.0 37.0 5 35.92838559432183 37.0 35.0 37.0 35.0 37.0 6 35.956235700816194 37.0 35.0 37.0 35.0 37.0 7 35.89046771346626 37.0 35.0 37.0 35.0 37.0 8 35.859157796923945 37.0 35.0 37.0 35.0 37.0 9 37.53925067609198 39.0 37.0 39.0 35.0 39.0 10-11 37.44936975876119 39.0 37.0 39.0 34.5 39.0 12-13 37.33582203162229 39.0 37.0 39.0 34.0 39.0 14-15 38.544815419349376 40.0 38.0 41.0 34.0 41.0 16-17 38.4823903711289 40.0 38.0 41.0 34.0 41.0 18-19 38.44120890129129 40.0 38.0 41.0 34.0 41.0 20-21 38.31362104523892 40.0 38.0 41.0 34.0 41.0 22-23 38.255146090443375 40.0 38.0 41.0 33.5 41.0 24-25 38.289910251751806 40.0 38.0 41.0 34.0 41.0 26-27 38.11741021225626 40.0 38.0 41.0 33.0 41.0 28-29 37.96015240255012 40.0 37.0 41.0 33.0 41.0 30-31 38.03006419103781 40.0 37.0 41.0 33.0 41.0 32-33 37.94552145613625 40.0 37.0 41.0 33.0 41.0 34-35 37.8141580051732 40.0 37.0 41.0 33.0 41.0 36-37 37.632819220975435 40.0 37.0 41.0 33.0 41.0 38-39 37.50740810822067 40.0 37.0 41.0 32.5 41.0 40-41 37.315670505155246 40.0 36.5 41.0 32.0 41.0 42-43 37.12597841245129 39.5 36.0 41.0 31.0 41.0 44-45 36.848977998825184 39.0 36.0 41.0 31.0 41.0 46-47 36.55369114624699 39.0 36.0 41.0 30.0 41.0 48-49 36.249162694371954 39.0 35.0 40.0 30.0 41.0 50-51 35.99321969147513 39.0 35.0 40.0 29.0 41.0 52-53 35.73436945715882 38.5 35.0 40.0 28.5 41.0 54-55 35.49888963651169 38.0 35.0 40.0 28.0 41.0 56-57 35.15793641503933 38.0 34.0 40.0 27.0 41.0 58-59 34.862815982340464 38.0 34.0 40.0 27.0 41.0 60-61 34.479987785104 37.5 34.0 40.0 26.0 41.0 62-63 34.068971973958796 37.0 33.0 39.5 26.0 41.0 64-65 33.619459133596926 36.0 33.0 39.0 25.0 40.5 66-67 33.138721112596784 36.0 32.0 39.0 23.5 40.0 68-69 32.65509614652704 35.0 31.5 38.0 22.5 40.0 70-71 32.19070335827058 35.0 31.0 37.5 22.0 40.0 72-73 31.68995150664746 35.0 31.0 37.0 20.5 39.0 74-75 31.183725572003254 34.0 30.0 36.5 19.0 39.0 76-77 29.90354792102614 33.5 28.5 35.0 18.0 37.0 78-79 30.559210469623615 34.0 30.0 35.5 18.0 37.5 80-81 30.380807640880622 34.0 30.0 35.0 18.0 37.0 82-83 29.965676573107697 34.0 30.0 35.0 13.5 36.5 84-85 29.504094252176923 34.0 29.0 35.0 7.5 36.0 86-87 29.089418998938648 34.0 29.0 35.0 4.5 36.0 88-89 28.591091994467753 33.5 29.0 35.0 2.0 35.0 90-91 27.97776418572387 33.0 27.0 35.0 2.0 35.0 92-93 27.409625926888715 33.0 26.0 35.0 2.0 35.0 94-95 26.8413851591527 32.0 25.5 34.0 2.0 35.0 96-97 26.08206528685976 32.0 24.5 34.0 2.0 35.0 98-99 24.993426684054285 32.0 20.5 34.0 2.0 35.0 100-101 22.02051991221935 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23330110073973032 1101 2 0.22525232500375836 1101 3 0.18653005621221297 1101 4 0.14673728321130852 1101 5 0.1271031912904519 1101 6 0.09251248096889952 1101 7 0.11031114882016624 1101 8 0.08714872718112332 1101 9 0.18931553326615358 1101 10-11 0.1935443215197452 1101 12-13 0.1983350385966247 1101 14-15 0.30487268340676366 1101 16-17 0.18538950049789094 1101 18-19 0.21780262565103925 1101 20-21 0.24679456490216722 1101 22-23 0.2714119142528659 1101 24-25 0.2543629150679507 1101 26-27 0.12644327043032177 1101 28-29 0.09824362374531859 1101 30-31 0.24659763230010157 1101 32-33 0.1746082506324811 1101 34-35 0.17450190400582954 1101 36-37 0.29593071992780295 1101 38-39 0.10757375222328847 1101 40-41 0.1127027350950982 1101 42-43 0.1819701525249826 1101 44-45 0.26531040949606677 1101 46-47 0.28985639916136563 1101 48-49 0.3219897731177568 1101 50-51 0.33886812589001636 1101 52-53 0.34572293367682505 1101 54-55 0.24671227919712635 1101 56-57 0.3797795036323137 1101 58-59 0.4559308541416911 1101 60-61 0.45656932622573265 1101 62-63 0.3482876112389377 1101 64-65 0.3775097094817994 1101 66-67 0.411270215494703 1101 68-69 0.39230828889126457 1101 70-71 0.3652526164847032 1101 72-73 0.33687090706439804 1101 74-75 0.39481014735786957 1101 76-77 0.2849864504545998 1101 78-79 0.4650241749956052 1101 80-81 0.4862542532198937 1101 82-83 0.36397268769473 1101 84-85 0.4917456481978668 1101 86-87 0.6513667651759683 1101 88-89 1.0016455104044368 1101 90-91 1.0642663075730212 1101 92-93 1.1498834223131915 1101 94-95 1.247748358995807 1101 96-97 1.51811379060225 1101 98-99 2.010749420917019 1101 100-101 2.559711150852344 1102 1 0.21267809597774345 1102 2 0.2260060167065845 1102 3 0.20045024400797473 1102 4 0.14620762172056345 1102 5 0.10674635906969598 1102 6 0.12704652943635608 1102 7 0.1274695372855703 1102 8 0.11979074409709511 1102 9 0.1695753307861807 1102 10-11 0.1623704777526953 1102 12-13 0.15620553104464818 1102 14-15 0.2213518548090505 1102 16-17 0.3139714618118319 1102 18-19 0.21871986736005766 1102 20-21 0.27107500699508336 1102 22-23 0.28309801194573936 1102 24-25 0.14740623032547262 1102 26-27 0.2507119544158414 1102 28-29 0.16577282992000875 1102 30-31 0.2199738679822758 1102 32-33 0.28737829077602584 1102 34-35 0.36786442752826076 1102 36-37 0.35744833278749155 1102 38-39 0.2969468184842512 1102 40-41 0.3796112762022119 1102 42-43 0.3602238559063977 1102 44-45 0.3894052066776581 1102 46-47 0.45526543867109126 1102 48-49 0.4750331201808038 1102 50-51 0.5772951614663953 1102 52-53 0.5443702444247194 1102 54-55 0.6674502609552633 1102 56-57 0.6758481014049522 1102 58-59 0.6073196178239044 1102 60-61 0.7080859282658807 1102 62-63 0.6746825347580696 1102 64-65 0.5323859993824698 1102 66-67 0.5847144255610957 1102 68-69 0.5297612601888559 1102 70-71 0.39404084660925065 1102 72-73 0.5651433309828313 1102 74-75 0.48551942362995604 1102 76-77 0.4035550365486458 1102 78-79 0.4647445347996566 1102 80-81 0.5181965484442053 1102 82-83 0.5376353620051937 1102 84-85 0.5858558303113703 1102 86-87 0.707925001153729 1102 88-89 0.770515508574622 1102 90-91 1.109438411776292 1102 92-93 1.1088838062655917 1102 94-95 0.9915844718509774 1102 96-97 1.28216510167098 1102 98-99 1.7728836535782584 1102 100-101 2.4149116484498876 1103 1 0.15612263444628383 1103 2 0.14810913174116536 1103 3 0.10984091772619564 1103 4 0.06931933160316817 1103 5 0.10628497031751749 1103 6 0.02816460407314736 1103 7 0.03164965188354785 1103 8 0.06225368643247009 1103 9 0.08883644538569513 1103 10-11 0.16477427940316147 1103 12-13 0.14399102316950518 1103 14-15 0.1512678675925585 1103 16-17 0.14936024909127354 1103 18-19 0.18542071311408392 1103 20-21 0.16868175412820818 1103 22-23 0.07183070576951422 1103 24-25 0.15702659561036114 1103 26-27 0.0999406686290385 1103 28-29 0.16705676344762566 1103 30-31 0.13096281740828175 1103 32-33 0.106190686734557 1103 34-35 0.21666289793535043 1103 36-37 0.06817735208617393 1103 38-39 0.11291268208057659 1103 40-41 0.09668687376091611 1103 42-43 0.08001668126790662 1103 44-45 0.21486372199519366 1103 46-47 0.3251136499676548 1103 48-49 0.16746785850154566 1103 50-51 0.0948744586204242 1103 52-53 0.1196515324639833 1103 54-55 0.1724716362235128 1103 56-57 0.1665212507201872 1103 58-59 0.12836135552290528 1103 60-61 0.14375758493788027 1103 62-63 0.0325061934233446 1103 64-65 0.1299869803614797 1103 66-67 0.22390446797222552 1103 68-69 0.24533192045399943 1103 70-71 0.19854797349858444 1103 72-73 0.08894737469606895 1103 74-75 0.18727267196105046 1103 76-77 0.1604239435172481 1103 78-79 0.23620123310367092 1103 80-81 0.2672051652768985 1103 82-83 0.19685869190326244 1103 84-85 0.3727559381519505 1103 86-87 0.44416067062157794 1103 88-89 0.5346341972393276 1103 90-91 0.6973646925567536 1103 92-93 0.653472135646826 1103 94-95 0.6723625150304073 1103 96-97 0.8488685123029214 1103 98-99 1.232347156329812 1103 100-101 1.879446763364129 1104 1 0.1495553823580451 1104 2 0.15322264641239514 1104 3 0.18149169947817256 1104 4 0.11455921166314198 1104 5 0.1289745184006179 1104 6 0.0920353472729758 1104 7 0.15740730876938613 1104 8 0.1413471754022737 1104 9 0.21193561008905704 1104 10-11 0.20600232609410085 1104 12-13 0.24761331916437257 1104 14-15 0.24300726575062725 1104 16-17 0.19291748832879563 1104 18-19 0.18158423849425276 1104 20-21 0.25104280574525717 1104 22-23 0.20973362930774897 1104 24-25 0.0993951434792919 1104 26-27 0.22667417687491564 1104 28-29 0.17772687482051452 1104 30-31 0.19639572595401233 1104 32-33 0.2299645141065767 1104 34-35 0.2098185522510505 1104 36-37 0.26004391880281474 1104 38-39 0.35819807509835044 1104 40-41 0.4064627893745296 1104 42-43 0.36885663629040266 1104 44-45 0.28189627714618126 1104 46-47 0.3203896726705935 1104 48-49 0.36060477218756404 1104 50-51 0.34686230398344975 1104 52-53 0.37618398052566704 1104 54-55 0.3495652322826288 1104 56-57 0.2622229534402578 1104 58-59 0.2990643968593787 1104 60-61 0.4202447877650357 1104 62-63 0.5178309417634566 1104 64-65 0.3773860486844711 1104 66-67 0.4650615679936365 1104 68-69 0.6184685664167304 1104 70-71 0.5777565834793066 1104 72-73 0.5497272526142574 1104 74-75 0.6584910806138673 1104 76-77 0.6617371262116158 1104 78-79 0.554498780758415 1104 80-81 0.6332913186287961 1104 82-83 0.5320736736980791 1104 84-85 0.6629304937313059 1104 86-87 0.7009394241019784 1104 88-89 0.9144558935340363 1104 90-91 1.000099485874383 1104 92-93 0.9842107841797016 1104 94-95 1.1625384092261655 1104 96-97 1.2410402835601495 1104 98-99 1.5074368436290193 1104 100-101 2.1919132979539775 1105 1 0.029132415152631097 1105 2 0.02167228869691229 1105 3 -0.01880509914436601 1105 4 0.012564952881128022 1105 5 1.8168644185578842E-4 1105 6 -0.09001933026703313 1105 7 -0.06866663420525043 1105 8 -0.04153435599605615 1105 9 0.1309940395693161 1105 10-11 0.020148590286162005 1105 12-13 0.06428116004190088 1105 14-15 0.11767765279595466 1105 16-17 0.13648639528507545 1105 18-19 0.16625306840136034 1105 20-21 0.1542518118378382 1105 22-23 0.009703877646614956 1105 24-25 -0.055904982572620554 1105 26-27 0.014743626137118326 1105 28-29 0.026479705827931355 1105 30-31 -0.08984882013571394 1105 32-33 -0.04898320149984414 1105 34-35 -0.08460043101397474 1105 36-37 0.02063454536920517 1105 38-39 -0.03066573902870573 1105 40-41 -0.051124714389302994 1105 42-43 -0.17647560629827552 1105 44-45 0.011854719056621832 1105 46-47 0.05784503956960663 1105 48-49 0.08222556909231571 1105 50-51 0.09037202173756498 1105 52-53 0.034348837037846636 1105 54-55 0.1229904921160454 1105 56-57 0.28378554837744474 1105 58-59 0.14611501103149038 1105 60-61 0.2861796293834331 1105 62-63 0.2981173474980423 1105 64-65 0.39624375541924906 1105 66-67 0.37762188466260227 1105 68-69 0.3927401106805135 1105 70-71 0.4808863415574649 1105 72-73 0.40051760122922886 1105 74-75 0.2889293634179886 1105 76-77 0.44337931918277107 1105 78-79 0.5384150237742915 1105 80-81 0.48104076028031173 1105 82-83 0.437873677018775 1105 84-85 0.5124539062244011 1105 86-87 0.6895659429287697 1105 88-89 0.8877172724225666 1105 90-91 1.0828133668865831 1105 92-93 1.0227598275269294 1105 94-95 1.0182979134907946 1105 96-97 1.401332451154893 1105 98-99 1.4340898445754213 1105 100-101 1.9344669687390592 1106 1 -0.17291380970428705 1106 2 -0.006581755341876772 1106 3 -0.0362100071272593 1106 4 0.05749440865511701 1106 5 -0.05721827744686436 1106 6 -0.05276170297175753 1106 7 -0.08380428723889821 1106 8 -0.13827016323454444 1106 9 -0.1896631856547728 1106 10-11 -0.2912348230049773 1106 12-13 -0.3590211555633829 1106 14-15 -0.12006481079285436 1106 16-17 -0.11573511467521769 1106 18-19 -0.04656594988088614 1106 20-21 0.021465317939792783 1106 22-23 0.006324807402911858 1106 24-25 0.025345388537388658 1106 26-27 0.022087666019473318 1106 28-29 -0.02318238714769194 1106 30-31 -0.139089816855396 1106 32-33 -0.04286973211505796 1106 34-35 -0.41641034513457953 1106 36-37 0.05291596611767346 1106 38-39 0.10199836430285814 1106 40-41 -0.08626110716365076 1106 42-43 -0.0914141625985394 1106 44-45 -0.13817551504865122 1106 46-47 -0.07974316343393184 1106 48-49 -0.02707041855714465 1106 50-51 0.07731076707672457 1106 52-53 -0.0357421803428295 1106 54-55 -0.09109242082790558 1106 56-57 0.04074272500884746 1106 58-59 -0.02194238408524285 1106 60-61 -0.05239872542075119 1106 62-63 -0.025259129519803025 1106 64-65 -0.021111105728053303 1106 66-67 -0.1619569221384367 1106 68-69 -0.005081337236241268 1106 70-71 0.10250439331776562 1106 72-73 0.09260959531085788 1106 74-75 -0.002958807957885057 1106 76-77 0.038730052980863405 1106 78-79 0.11560348222269567 1106 80-81 0.17611132874208124 1106 82-83 0.1956027624704184 1106 84-85 0.3484408528463625 1106 86-87 0.47197914559906096 1106 88-89 0.6578059874064444 1106 90-91 0.4688507844698684 1106 92-93 0.5696850977859 1106 94-95 0.2556313987015315 1106 96-97 0.5824977547622652 1106 98-99 0.655650699952183 1106 100-101 1.2710628387239211 1107 1 -0.002295301099984215 1107 2 0.05160344703273978 1107 3 0.03117993527646945 1107 4 0.04788371971740446 1107 5 -0.028851537143893324 1107 6 0.037188092660230154 1107 7 -0.0067091364500839745 1107 8 0.036286655677095325 1107 9 0.04149987614519546 1107 10-11 -0.02639436092605507 1107 12-13 0.001874694911698782 1107 14-15 -0.043202323259116326 1107 16-17 1.4996906538300436E-4 1107 18-19 0.004624813387891891 1107 20-21 0.05293484087106748 1107 22-23 0.07375655648528578 1107 24-25 -0.014599984069178618 1107 26-27 0.04602168659805983 1107 28-29 0.03994872707905728 1107 30-31 0.09153399937940065 1107 32-33 0.15843972576744392 1107 34-35 0.08017059562475026 1107 36-37 0.05505537745541034 1107 38-39 0.09399753544001044 1107 40-41 0.10751042479058981 1107 42-43 0.07359922159756849 1107 44-45 -0.07823806753421536 1107 46-47 0.10169418653159568 1107 48-49 0.20868471306118153 1107 50-51 0.19572969907967774 1107 52-53 0.24012555059665885 1107 54-55 0.18584592462127603 1107 56-57 0.21778734424695045 1107 58-59 0.4190871750542371 1107 60-61 0.540444819446293 1107 62-63 0.46079319305325583 1107 64-65 0.5801707233929108 1107 66-67 0.5676847400951104 1107 68-69 0.5961223315724666 1107 70-71 0.6435621871126926 1107 72-73 0.5658872510346775 1107 74-75 0.5735746791917116 1107 76-77 0.40925779867992773 1107 78-79 0.46628093218494726 1107 80-81 0.5660452499540618 1107 82-83 0.6265896303261727 1107 84-85 0.6404096081100654 1107 86-87 0.7738221536441223 1107 88-89 1.0071425941825183 1107 90-91 1.0326786766740739 1107 92-93 1.1182402226182226 1107 94-95 1.0669360457865125 1107 96-97 1.1443734568418122 1107 98-99 1.6160712872572525 1107 100-101 2.117196690379288 1108 1 -0.02780278210958187 1108 2 0.005441093456028057 1108 3 -0.034492348753886404 1108 4 -0.022031321981287988 1108 5 -0.037304647597352414 1108 6 -0.04724990488593761 1108 7 -0.0829337283973004 1108 8 -0.08980037982286149 1108 9 -0.07982793174478076 1108 10-11 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82-83 0.6903101483911804 2316 84-85 0.5987004365820034 2316 86-87 0.7420933340341271 2316 88-89 0.739962552975129 2316 90-91 0.9968471211787389 2316 92-93 1.0996410963896182 2316 94-95 1.1408025268822755 2316 96-97 1.4980124816116387 2316 98-99 1.8677773974114196 2316 100-101 2.459317509189294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 23.0 5 44.0 6 131.0 7 261.0 8 792.0 9 1456.0 10 2426.0 11 3932.0 12 5838.0 13 8212.0 14 10647.0 15 14266.0 16 18046.0 17 23027.0 18 21805.0 19 16384.0 20 15991.0 21 16064.0 22 17770.0 23 19568.0 24 23159.0 25 27890.0 26 34079.0 27 41764.0 28 52243.0 29 64153.0 30 78895.0 31 97944.0 32 123672.0 33 159621.0 34 214222.0 35 298836.0 36 433880.0 37 568601.0 38 347538.0 39 21925.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.978976513092626 19.51457900941617 15.402843601895736 23.103600875595472 2 15.996487050486802 20.511941299561528 38.99025680723731 24.501314842714365 3 19.625445761122307 23.650867968535692 29.569204100068795 27.15448217027321 4 13.618316455568943 17.021827394399676 36.16804853212999 33.19180761790139 5 14.15597252946311 36.97021966591421 34.14076298349822 14.733044821124466 6 32.90844500998878 36.10920016487596 16.69598911933544 14.286365705799813 7 29.098740307433353 32.15842454411366 20.181507178854513 18.56132796959848 8 26.695687516965133 34.929324511224635 19.30265941480523 19.072328557005 9 26.044118681968918 16.830417604386735 19.982916380024733 37.14254733361962 10-11 25.096261423189777 26.571707399742344 26.93027312248934 21.40175805457854 12-13 26.00642412319121 23.55851756472194 27.536888618070236 22.89816969401662 14-15 22.799182370336112 25.289035604877913 25.629451327840343 26.28233069694563 16-17 22.482069020984344 28.33301138477138 26.451066451619386 22.733853142624888 18-19 22.49506664694283 26.983161252425386 28.573234082216807 21.94853801841498 20-21 22.924563919070618 26.09062196433582 28.94003291420709 22.044781202386467 22-23 22.81598506337295 25.843362895407708 28.821801730638036 22.518850310581307 24-25 22.7466198512345 26.145125087002484 28.795404437472477 22.31285062429054 26-27 22.444855924523207 26.39474152875262 28.725402754977615 22.434999791746556 28-29 22.498751801830835 26.615265378331067 28.616547597867086 22.26943522197102 30-31 22.508681414224505 26.296512192577943 28.603794491181965 22.59101190201559 32-33 22.497444902254607 26.23416202937146 28.59623387658371 22.67215919179022 34-35 22.995708629131055 26.357551764302055 28.36351271972092 22.283226886845974 36-37 23.055381415966696 26.090526734555976 28.42929322306168 22.424798626415647 38-39 22.63993198141122 26.424794470330013 28.39600146636358 22.539272081895184 40-41 22.96165269481718 26.324824316619296 28.25822117794396 22.455301810619563 42-43 22.77143054636661 26.301021850893974 28.128647463685823 22.79890013905359 44-45 22.78157094621281 26.534707251813565 28.183825926601475 22.499895875372154 46-47 23.105596016550788 26.797460364421838 27.62049373744221 22.476449881585165 48-49 23.112687262757422 26.768233658537287 27.50620798212216 22.61287109658313 50-51 23.107786224239927 26.98953439775033 27.415098932758468 22.48758044525127 52-53 23.249974372770186 26.941893240999903 27.220354860385903 22.587777525844007 54-55 23.041631180514763 26.961007840225427 27.318352791473554 22.679008187786252 56-57 23.077087411547925 27.12143157719369 27.239128311701744 22.562352699556644 58-59 23.142057588818307 26.75234567653002 27.35713023908451 22.74846649556716 60-61 23.027430735579458 26.93657445772612 27.45737712216763 22.578617684526794 62-63 22.803437118060092 26.991078314645954 27.62049373744221 22.58499082985174 64-65 22.776687860217354 26.92903439846843 27.524842700001866 22.769435041312345 66-67 22.854978808842983 26.95976911620452 27.30181851867278 22.88343355627972 68-69 22.88241026252332 26.856003538801144 27.48730397641182 22.77428222226371 70-71 22.97163429707259 26.75367416316114 27.278480240018038 22.996211299748236 72-73 22.942179490355706 26.791763655341118 27.351524297525525 22.914532556777644 74-75 22.870705218151866 27.250240922845805 26.95831496191911 22.92073889708322 76-77 23.0661184205802 26.890831431562834 27.229110804402247 22.813939343454727 78-79 22.92660937179099 26.959392113241638 27.233975937875655 22.880022577091722 80-81 22.98450625537678 27.07145175582847 27.025852349841518 22.918189638953237 82-83 23.014953588134073 27.03663310434838 27.042952406855363 22.90546090066218 84-85 22.964451826526812 27.126854766146693 26.988620247156135 22.92007316017036 86-87 23.035814563373123 27.113801755325795 26.920165852579636 22.93021782872145 88-89 22.94646486116956 27.053391518654106 26.792218227985543 23.207925392190795 90-91 22.981023212523645 27.006031866409852 26.904842441817877 23.108102479248625 92-93 22.94523812644172 26.837434613766792 27.0873730806469 23.12995417914459 94-95 22.831317384791486 26.83225235213944 27.04037594017358 23.2960543228955 96-97 22.809487117988283 26.75410502369015 27.08486228979392 23.351545568527648 98-99 22.652656961987596 26.930215139974372 26.85388082439625 23.56324707364179 100-101 22.79968988929953 26.511574210395533 27.236326904652806 23.452408995652128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 186.0 2 143.5 3 221.0 4 217.5 5 195.5 6 342.5 7 792.0 8 1375.0 9 1707.0 10 1684.5 11 1664.0 12 1808.0 13 1974.5 14 2253.5 15 2443.5 16 2592.0 17 2818.5 18 3115.0 19 3487.5 20 3911.0 21 4540.5 22 5425.0 23 6678.5 24 8386.0 25 10722.5 26 13782.5 27 17299.5 28 20660.5 29 24483.0 30 28995.0 31 34110.5 32 40148.5 33 44560.0 34 48331.0 35 53193.0 36 59673.5 37 66588.0 38 71913.5 39 75728.5 40 79320.5 41 84794.0 42 88216.5 43 90739.0 44 95627.0 45 100152.5 46 102859.0 47 106762.0 48 109944.0 49 110321.5 50 108346.5 51 104323.5 52 102654.5 53 103579.0 54 104800.5 55 98546.5 56 87413.0 57 78243.5 58 70889.5 59 62268.0 60 50901.0 61 41863.0 62 34704.0 63 26439.0 64 18731.0 65 13444.5 66 9788.5 67 7739.5 68 6167.0 69 4701.5 70 3553.0 71 2499.0 72 1798.5 73 1484.5 74 1128.5 75 752.0 76 508.5 77 321.0 78 201.5 79 124.5 80 77.0 81 42.0 82 26.0 83 15.0 84 10.5 85 8.5 86 6.5 87 4.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04272700246021362 2 0.0 3 0.0 4 0.0 5 0.001113056366610607 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005475519222842502 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.6157269837895906E-4 22-23 8.61721058021115E-4 24-25 8.976261021053282E-5 26-27 0.002010682468715935 28-29 0.003931602327221337 30-31 2.6928783063159844E-4 32-33 1.9747774246317218E-4 34-35 0.0 36-37 7.181008816842625E-5 38-39 1.0771513225263938E-4 40-41 0.0 42-43 0.008132492485074274 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.795252204210656E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.7952522042106564E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3338278654738532E-4 84-85 7.181008816842625E-5 86-87 0.0 88-89 0.0 90-91 0.008904450932884856 92-93 0.0013823441972422054 94-95 0.0 96-97 0.0 98-99 2.513353085894919E-4 100-101 0.011202373754274495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2785124.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.7335426990079 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01821228805491 37.774908032093116 2 14.576197012844306 15.081566211062643 3 5.1744315586619525 8.030750279493963 4 2.39036705384423 4.946486241854087 5 1.3074982994525297 3.3820759551803823 6 0.8027977227836788 2.491894216217745 7 0.5037072072085541 1.8240990818345226 8 0.366579013140172 1.5171544823077812 9 0.2651963418711301 1.2347591648229723 >10 1.4426010151055952 13.599185210761334 >50 0.10186973355429195 3.5961865286379484 >100 0.04692991293920215 4.293385947932897 >500 0.002501197296621159 0.9078193467888409 >1k 9.726878375602675E-4 0.8084721291951636 >5k 1.3895540536575253E-4 0.5112571718166543 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7725 0.2773664655505464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6499 0.2333468815033011 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4190 0.150442134712853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.590504408421313E-5 2 0.0 0.0 0.0 0.0 3.590504408421313E-5 3 0.0 0.0 0.0 0.0 3.590504408421313E-5 4 0.0 0.0 0.0 0.0 3.590504408421313E-5 5 0.0 0.0 0.0 0.0 3.590504408421313E-5 6 0.0 0.0 0.0 0.0 3.590504408421313E-5 7 0.0 0.0 0.0 0.0 3.590504408421313E-5 8 0.0 0.0 0.0 0.0 7.181008816842626E-5 9 0.0 0.0 0.0 0.0 7.181008816842626E-5 10-11 0.0 0.0 0.0 0.0 7.181008816842626E-5 12-13 0.0 0.0 0.0 0.0 1.7952522042106564E-4 14-15 0.0 0.0 0.0 0.0 1.7952522042106564E-4 16-17 0.0 0.0 0.0 5.3857566126319695E-5 1.7952522042106564E-4 18-19 0.0 0.0 0.0 7.181008816842626E-5 2.1543026450527875E-4 20-21 0.0 0.0 0.0 1.974777424631722E-4 2.513353085894919E-4 22-23 0.0 0.0 0.0 2.8724035267370504E-4 3.051928747158116E-4 24-25 0.0 0.0 0.0 4.1290800696845096E-4 3.2314539675791813E-4 26-27 0.0 0.0 0.0 7.00148359642156E-4 3.5905044084213127E-4 28-29 3.590504408421313E-5 0.0 0.0 0.001364391675200099 3.5905044084213127E-4 30-31 3.590504408421313E-5 0.0 0.0 0.0029442136149054766 3.770029628842378E-4 32-33 3.590504408421313E-5 0.0 0.0 0.005044658693831944 4.308605290105575E-4 34-35 3.590504408421313E-5 0.0 0.0 0.00752210673564265 4.308605290105575E-4 36-37 3.590504408421313E-5 0.0 0.0 0.010771513225263938 4.308605290105575E-4 38-39 7.181008816842626E-5 3.590504408421313E-5 0.0 0.013823441972422055 4.488130510526641E-4 40-41 7.181008816842626E-5 3.590504408421313E-5 0.0 0.016067507227685372 4.6676557309477065E-4 42-43 7.181008816842626E-5 3.590504408421313E-5 0.0 0.018975815798506636 5.026706171789838E-4 44-45 7.181008816842626E-5 3.590504408421313E-5 0.0 0.02256632020692795 5.385756612631969E-4 46-47 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.027970029341602025 5.744807053474101E-4 48-49 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.03436112718859196 6.642433155579429E-4 50-51 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.039998219109813424 6.821958376000495E-4 52-53 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.05233160175274063 7.00148359642156E-4 54-55 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.07744718008964771 7.181008816842625E-4 56-57 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.11952789175634548 7.360534037263691E-4 58-59 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.16641987933032784 7.899109698526888E-4 60-61 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.20970340997384676 7.899109698526888E-4 62-63 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.2642790769818507 7.899109698526888E-4 64-65 1.0771513225263938E-4 3.590504408421313E-5 0.0 0.3359096399298559 8.258160139369019E-4 66-67 1.4362017633685252E-4 3.590504408421313E-5 0.0 0.4402496980385793 8.258160139369019E-4 68-69 1.4362017633685252E-4 3.590504408421313E-5 0.0 0.5692744739551991 8.258160139369019E-4 70-71 1.4362017633685252E-4 3.590504408421313E-5 0.0 0.6954088938230398 8.437685359790085E-4 72-73 1.4362017633685252E-4 3.590504408421313E-5 0.0 0.8230333730203754 8.976261021053282E-4 74-75 1.4362017633685252E-4 3.590504408421313E-5 0.0 0.9627758045961329 8.976261021053282E-4 76-77 1.4362017633685252E-4 3.590504408421313E-5 0.0 1.1245818857616392 9.155786241474347E-4 78-79 1.4362017633685252E-4 3.590504408421313E-5 0.0 1.276998797899124 9.335311461895413E-4 80-81 1.4362017633685252E-4 3.590504408421313E-5 0.0 1.4450703092573258 9.694361902737544E-4 82-83 1.4362017633685252E-4 3.590504408421313E-5 0.0 1.6397474582819291 0.0010053412343579676 84-85 1.4362017633685252E-4 3.590504408421313E-5 0.0 1.8918906303633158 0.0010053412343579676 86-87 1.7952522042106564E-4 3.590504408421313E-5 0.0 2.1545360278393346 0.0010412462784421808 88-89 1.7952522042106564E-4 3.590504408421313E-5 0.0 2.496118664734497 0.0010771513225263938 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6455 0.0 38.873432 1 GTATCAA 10050 0.0 35.229412 1 ATCAACG 12485 0.0 28.076387 3 TCAACGC 12535 0.0 27.964396 4 CAACGCA 12760 0.0 27.508518 5 TATCAAC 12840 0.0 27.374117 2 AACGCAG 13145 0.0 26.703308 6 CGCAGAG 15115 0.0 23.038128 8 ACGCAGA 15220 0.0 23.004045 7 GAGTACT 9380 0.0 21.448557 12-13 GTACATG 8795 0.0 21.01982 1 TACATGG 8640 0.0 20.835258 2 AGAGTAC 13555 0.0 20.607685 10-11 GCAGAGT 16875 0.0 20.35382 9 AGTACTT 9810 0.0 20.314703 12-13 GTACTTT 9965 0.0 19.971474 14-15 ACATGGG 8745 0.0 19.879007 3 CAGAGTA 16075 0.0 18.071611 10-11 TAAGGTG 1360 0.0 17.811672 5 TACTTTT 11105 0.0 17.664646 14-15 >>END_MODULE