FastQCFastQC Report
Thu 26 May 2016
SRR937506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740761
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70210.25616972804268595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.20373903452362319No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39820.1452881152351482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34470.1257679892555389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92750.038.102161
GGTATCA55800.038.1020281
ATCAACG112550.031.0411913
TCAACGC112800.030.9330864
TATCAAC114100.030.6617322
CAACGCA114550.030.500345
AACGCAG119650.029.2583266
CGCAGAG134950.025.5544418
ACGCAGA136350.025.3965687
GCAGAGT149550.022.4561679
TAAGGTG11900.021.9699045
GTACATG86600.021.6947151
GTATAGG7700.021.619821
TACATGG83800.021.5548022
GAGTACT79000.021.37427312-13
AGAGTAC119650.021.35798510-11
ACATGGG86600.020.0341783
AGTACTT82950.020.0128812-13
GTACTTT84600.019.87448314-15
CAGAGTA141050.018.77424610-11
Produced by FastQC (version 0.11.5)