FastQCFastQC Report
Thu 26 May 2016
SRR937506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740761
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.28430060118339395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66240.2416846999793123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.14933808529820733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69250.037.7449651
GTATCAA99850.031.5083751
ATCAACG123600.025.249743
TCAACGC123900.025.2269444
TATCAAC125400.024.9251862
CAACGCA126500.024.7084455
AACGCAG130900.023.9483016
GTATAGG8100.021.708651
GTACATG84100.021.5300541
TAAGGTG15500.021.4524255
TACATGG82000.021.3758092
GTAAGGT12900.021.3573974
GAGTACT89750.020.77566112-13
ACGCAGA149850.020.765117
CGCAGAG149600.020.6727898
ACATGGG82100.020.0190283
GTACTTT92200.019.98808714-15
AGAGTAC126950.019.85191310-11
AGTACTT93150.018.92085312-13
GCAGAGT164350.018.6440249
Produced by FastQC (version 0.11.5)