##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2740761 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0597293963246 31.0 31.0 34.0 30.0 34.0 2 32.29992509379694 34.0 31.0 34.0 31.0 34.0 3 32.38006123116901 34.0 31.0 34.0 30.0 34.0 4 36.02018453998725 37.0 35.0 37.0 35.0 37.0 5 35.8503860789029 37.0 35.0 37.0 35.0 37.0 6 35.932262608815584 37.0 35.0 37.0 35.0 37.0 7 35.89062636253216 37.0 35.0 37.0 35.0 37.0 8 35.857726375995576 37.0 35.0 37.0 35.0 37.0 9 37.54869906569745 39.0 37.0 39.0 35.0 39.0 10-11 37.46465215317936 39.0 37.0 39.0 34.5 39.0 12-13 37.37490572873739 39.0 37.0 39.0 34.0 39.0 14-15 38.58376560378669 40.0 38.0 41.0 34.0 41.0 16-17 38.5498365235057 40.0 38.0 41.0 34.0 41.0 18-19 38.534313827436975 40.0 38.0 41.0 34.0 41.0 20-21 38.42707809984161 40.0 38.0 41.0 34.0 41.0 22-23 38.29155442594228 40.0 38.0 41.0 34.0 41.0 24-25 38.142394393381984 40.0 37.5 41.0 33.0 41.0 26-27 37.90755614955117 40.0 37.0 41.0 33.0 41.0 28-29 37.89748175780376 40.0 37.0 41.0 33.0 41.0 30-31 38.05095628549881 40.0 37.0 41.0 33.0 41.0 32-33 38.03157225310781 40.0 37.0 41.0 33.0 41.0 34-35 37.92239454662409 40.0 37.0 41.0 33.0 41.0 36-37 37.765123810503724 40.0 37.0 41.0 33.0 41.0 38-39 37.61713808683063 40.0 37.0 41.0 33.0 41.0 40-41 37.457466375214764 40.0 37.0 41.0 32.0 41.0 42-43 37.260174090334765 40.0 36.5 41.0 31.5 41.0 44-45 37.01331765155736 39.5 36.0 41.0 31.0 41.0 46-47 36.74111131908255 39.0 36.0 41.0 31.0 41.0 48-49 36.46082584362519 39.0 36.0 41.0 30.0 41.0 50-51 36.25087211179669 39.0 35.0 41.0 30.0 41.0 52-53 36.008812698371 39.0 35.0 40.0 29.0 41.0 54-55 35.79333513575244 39.0 35.0 40.0 29.0 41.0 56-57 35.5268998281864 38.0 35.0 40.0 28.0 41.0 58-59 35.2522005749498 38.0 34.0 40.0 28.0 41.0 60-61 34.8989676589823 38.0 34.0 40.0 27.0 41.0 62-63 34.50539466958264 37.0 34.0 40.0 26.0 41.0 64-65 34.14821759358076 36.5 33.5 39.5 26.0 41.0 66-67 33.740992921309086 36.0 33.0 39.0 26.0 40.5 68-69 33.289557170435515 35.5 32.5 39.0 25.0 40.0 70-71 32.898490419266764 35.0 32.0 38.0 24.5 40.0 72-73 32.45583817779077 35.0 32.0 37.5 23.5 39.5 74-75 32.012072741840676 35.0 31.0 37.0 22.5 39.0 76-77 30.716658621455863 33.5 29.5 35.5 21.0 38.0 78-79 31.35386649912196 34.0 31.0 36.0 22.0 38.0 80-81 31.201037412601828 34.0 31.0 35.5 21.0 37.0 82-83 30.866781525277105 34.0 31.0 35.0 20.0 37.0 84-85 30.536336805726584 34.0 31.0 35.0 19.5 36.5 86-87 30.205043051911495 34.0 30.0 35.0 18.0 36.0 88-89 29.83484550458796 34.0 30.0 35.0 15.0 36.0 90-91 29.380937082802916 34.0 29.5 35.0 7.5 35.0 92-93 28.943024400887197 34.0 29.0 35.0 3.5 35.0 94-95 28.51994956875116 33.5 29.0 35.0 2.0 35.0 96-97 28.06234874182754 33.0 29.0 35.0 2.0 35.0 98-99 27.371277356909268 33.0 27.0 35.0 2.0 35.0 100-101 25.715187679626204 31.5 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.032306730241714376 1101 2 0.10362371831676143 1101 3 0.09822254667898989 1101 4 0.05215782755455223 1101 5 -0.10089386288506574 1101 6 0.023185195724309438 1101 7 0.02629009035590002 1101 8 -0.0585724262954983 1101 9 0.01295210517816514 1101 10-11 0.019957550993005668 1101 12-13 0.0937612423743488 1101 14-15 0.16524486877094802 1101 16-17 0.06839483350821496 1101 18-19 0.1558069829754274 1101 20-21 0.06276864912010183 1101 22-23 -0.03238764944480721 1101 24-25 0.1141202668118737 1101 26-27 0.11473732056523289 1101 28-29 0.10018610216388169 1101 30-31 0.09473058093552567 1101 32-33 0.01799529057399951 1101 34-35 0.026174743442695103 1101 36-37 0.15649051616741616 1101 38-39 0.10077779629586558 1101 40-41 0.08967364224302088 1101 42-43 0.05633532107122363 1101 44-45 0.11537363403010659 1101 46-47 0.059830284204259954 1101 48-49 0.05626965933669226 1101 50-51 -0.006919754500138708 1101 52-53 0.210344647044856 1101 54-55 0.21124313638455305 1101 56-57 0.10240159766748036 1101 58-59 0.08849312382451302 1101 60-61 0.10424219466199247 1101 62-63 0.03916778606335214 1101 64-65 0.0744932767056099 1101 66-67 -0.05464298131884959 1101 68-69 -0.1609969401117155 1101 70-71 -0.20268154735679644 1101 72-73 -0.14417528222628562 1101 74-75 -0.08945722237823617 1101 76-77 -0.19195220832769166 1101 78-79 -0.12911166649246297 1101 80-81 -0.2410231793278932 1101 82-83 -0.25934516682293207 1101 84-85 -0.28304895873042923 1101 86-87 -0.3556527985833071 1101 88-89 -0.3636139894294921 1101 90-91 -0.20083257709401892 1101 92-93 -0.18140433423672775 1101 94-95 -0.3458929788633647 1101 96-97 -0.2655932078589558 1101 98-99 -0.24068815296812573 1101 100-101 -0.05618738835752879 1102 1 0.10178975239097099 1102 2 0.10009122775367985 1102 3 -0.04345268537503699 1102 4 -0.004621716452376745 1102 5 -0.09839753988176625 1102 6 0.011770226629856495 1102 7 0.06818313142980514 1102 8 0.056003864963855676 1102 9 0.056323782226343155 1102 10-11 0.08370952833723067 1102 12-13 0.04995982041845792 1102 14-15 0.10757346983056948 1102 16-17 0.09577611036969103 1102 18-19 0.11040322159720262 1102 20-21 0.15541159820256212 1102 22-23 0.0985000323719305 1102 24-25 0.11045541307559148 1102 26-27 0.08013310725578293 1102 28-29 -0.006586615657468542 1102 30-31 0.024563585634709284 1102 32-33 -0.004704751737712343 1102 34-35 -0.033622690588828164 1102 36-37 -0.08558097329853354 1102 38-39 -0.061182092050771075 1102 40-41 -0.015225188461869266 1102 42-43 0.14345897739875824 1102 44-45 0.04031162023859736 1102 46-47 0.1511890299122527 1102 48-49 0.21526898600677669 1102 50-51 0.04579760435916569 1102 52-53 0.15916881601844324 1102 54-55 0.06761685374138438 1102 56-57 0.12924805183899934 1102 58-59 0.07655876473464218 1102 60-61 -0.014858875554253359 1102 62-63 0.030794847791518976 1102 64-65 -0.014795069016933837 1102 66-67 0.03292938423069103 1102 68-69 -0.012869632282871635 1102 70-71 -5.821397110281623E-4 1102 72-73 0.10598627728280974 1102 74-75 -0.1514220549925227 1102 76-77 -0.23696197640089878 1102 78-79 -0.028743807173619018 1102 80-81 0.06390543309096941 1102 82-83 1.65219090220603E-4 1102 84-85 0.04244650091278501 1102 86-87 0.20948420262256917 1102 88-89 0.258768729590674 1102 90-91 0.06449409628175928 1102 92-93 -0.18645382480783823 1102 94-95 -0.2965012447306634 1102 96-97 -0.13374192601160928 1102 98-99 -0.18918063995507595 1102 100-101 -0.28665020180450185 1103 1 0.0807313833148271 1103 2 0.011828797893471688 1103 3 0.04197307348680113 1103 4 0.013251442260731494 1103 5 -0.14954935356343668 1103 6 -0.1092997854638611 1103 7 -0.026570092934903755 1103 8 -0.07389333845843993 1103 9 -0.043091625617293516 1103 10-11 -0.034230906360470215 1103 12-13 -0.017996341742481547 1103 14-15 -0.016584524039949144 1103 16-17 -0.12467405188139224 1103 18-19 -0.12173361953945516 1103 20-21 -0.12595929200001876 1103 22-23 -0.06760700263975394 1103 24-25 -0.037286357940054415 1103 26-27 -0.06584770430010423 1103 28-29 -0.0904509425783786 1103 30-31 -0.157526120059849 1103 32-33 -0.04583418077915269 1103 34-35 -0.11542319875020013 1103 36-37 -0.04341465842626491 1103 38-39 -0.04740591404029715 1103 40-41 -0.03767644741009235 1103 42-43 -0.11995622011940554 1103 44-45 -0.10268303899655962 1103 46-47 -0.1575036563948089 1103 48-49 -0.2898037546844421 1103 50-51 -0.42178109868379465 1103 52-53 -0.2385062893320864 1103 54-55 -0.3219261202608834 1103 56-57 -0.271878926535031 1103 58-59 -0.3331476622110543 1103 60-61 -0.40866488410247825 1103 62-63 -0.22861580894979028 1103 64-65 -0.32813233343362214 1103 66-67 -0.36552472350101795 1103 68-69 -0.3571691545435698 1103 70-71 -0.3823736808709768 1103 72-73 -0.39110442370196097 1103 74-75 -0.333585374604624 1103 76-77 -0.3991989287095947 1103 78-79 -0.1929561758140892 1103 80-81 -0.2788542062008368 1103 82-83 -0.42526012663228485 1103 84-85 -0.46581037034548345 1103 86-87 -0.31346775302880303 1103 88-89 -0.3241154242624127 1103 90-91 -0.23381883986192165 1103 92-93 -0.294408728771721 1103 94-95 -0.3309259857674185 1103 96-97 -0.11236698892146535 1103 98-99 -0.09833270903197189 1103 100-101 0.13362855457098988 1104 1 0.06018875901839493 1104 2 0.056015594328748364 1104 3 0.040225887745712896 1104 4 0.09906728419318966 1104 5 -0.1262877447454258 1104 6 -0.08779708846573442 1104 7 -0.07275178852589903 1104 8 0.005285542873643578 1104 9 -0.08198556323455364 1104 10-11 -0.06158125227979383 1104 12-13 -0.054708495823163616 1104 14-15 0.05557849777056134 1104 16-17 0.042452619084834 1104 18-19 -0.04556101165952242 1104 20-21 -0.18401437525518816 1104 22-23 -0.13947907112004998 1104 24-25 -0.15234264311828838 1104 26-27 -0.2322319679023508 1104 28-29 -0.3227203785539885 1104 30-31 -0.26547860012551894 1104 32-33 -0.2124031301224889 1104 34-35 -0.19481021798097942 1104 36-37 -0.13672419759137 1104 38-39 -0.17099365442491177 1104 40-41 -0.14741715449264348 1104 42-43 -0.12491268705186087 1104 44-45 -0.11157067384647235 1104 46-47 -0.14513484190137405 1104 48-49 -0.17702892471258025 1104 50-51 -0.07692884727666893 1104 52-53 -0.045133722493439166 1104 54-55 -0.08411024901323572 1104 56-57 -0.16236321358944394 1104 58-59 -0.10773431793338517 1104 60-61 -0.22523529920567142 1104 62-63 -0.331691705994821 1104 64-65 -0.38007446523474897 1104 66-67 -0.3575070171220389 1104 68-69 -0.3099130569825945 1104 70-71 -0.15091700873213654 1104 72-73 -0.06145943073761373 1104 74-75 -0.2829338443513407 1104 76-77 -0.22459321810922006 1104 78-79 -0.2797995154013293 1104 80-81 -0.20109931056293817 1104 82-83 -0.18579600843341026 1104 84-85 -0.26308039262503513 1104 86-87 -0.10054707995187684 1104 88-89 -0.02975346952507607 1104 90-91 -0.04824338254485028 1104 92-93 2.1339833897116023E-4 1104 94-95 -0.12504022709011764 1104 96-97 -0.1828715034618007 1104 98-99 -0.02352032629276124 1104 100-101 0.24721907989932035 1105 1 -0.062369609820851935 1105 2 -0.011953515528460912 1105 3 -0.02053240336437767 1105 4 -0.0034420001227957187 1105 5 -0.20403489467633307 1105 6 -0.08064672572967169 1105 7 -0.05085627666500159 1105 8 -0.12744598925441153 1105 9 -0.09864515270945162 1105 10-11 -0.04823612762525187 1105 12-13 -0.11607944383099067 1105 14-15 -0.12401022142566376 1105 16-17 -0.12632167127674876 1105 18-19 -0.06454707798840786 1105 20-21 -0.09328987473698902 1105 22-23 -0.07055993823198037 1105 24-25 -0.03556753947940905 1105 26-27 -0.0993532541759592 1105 28-29 -0.2078883239132736 1105 30-31 -0.2012633407404465 1105 32-33 -0.1252284410391198 1105 34-35 -0.06493667180409801 1105 36-37 -0.03092743774568163 1105 38-39 0.034293187756112786 1105 40-41 0.07167345035534822 1105 42-43 -0.0864963106700074 1105 44-45 -0.022472727911413415 1105 46-47 0.002961693393423559 1105 48-49 0.12101759043020621 1105 50-51 0.0475558372005338 1105 52-53 0.019481698107661316 1105 54-55 0.0844178990176303 1105 56-57 0.0368666919840237 1105 58-59 -0.04752804778137687 1105 60-61 7.863354097210618E-4 1105 62-63 0.017366007904307423 1105 64-65 0.11024213713743336 1105 66-67 -0.1520954844182114 1105 68-69 -0.08835617075504842 1105 70-71 -0.13436177784775083 1105 72-73 -0.07375467198588126 1105 74-75 0.017974615171922892 1105 76-77 0.06861087850523973 1105 78-79 0.020286850328744777 1105 80-81 0.00178512489307181 1105 82-83 -0.005598230911530777 1105 84-85 0.020561398312320733 1105 86-87 0.010334642180779952 1105 88-89 0.12110784870385416 1105 90-91 0.024375442305448303 1105 92-93 0.20128829182123908 1105 94-95 0.15226555797436703 1105 96-97 0.04431750793847655 1105 98-99 0.032197085038419004 1105 100-101 0.16028621486016448 1106 1 -0.14913382752298432 1106 2 -0.016981115584336237 1106 3 -0.09970184394398274 1106 4 -0.039948992197416544 1106 5 -0.11370978905600992 1106 6 -0.07060077703330592 1106 7 0.023947765659315223 1106 8 -0.01342773675607134 1106 9 -0.10873599798043188 1106 10-11 -0.10093708741425189 1106 12-13 -0.29722510464508645 1106 14-15 -0.1894656631626077 1106 16-17 -0.039881888013830746 1106 18-19 0.011483910625095461 1106 20-21 -0.1607773626511957 1106 22-23 -0.1750523166983129 1106 24-25 -0.07814237163878346 1106 26-27 0.006003859527559996 1106 28-29 -0.09319535299355408 1106 30-31 0.03168054126228981 1106 32-33 -0.052260079065703735 1106 34-35 -0.003012186935599459 1106 36-37 -0.03912124401378492 1106 38-39 -0.006492175779165166 1106 40-41 -0.09094665931927182 1106 42-43 -0.21146193531287594 1106 44-45 -0.032941792811001847 1106 46-47 -0.12921404903266165 1106 48-49 -0.19840716200372555 1106 50-51 -0.049968048684164046 1106 52-53 0.06355761776184465 1106 54-55 -0.042187811101058514 1106 56-57 -0.02731708828942203 1106 58-59 -0.09810090993347842 1106 60-61 -0.0868840674984952 1106 62-63 0.006639490626831446 1106 64-65 0.04056192772892331 1106 66-67 0.12131222908541162 1106 68-69 0.21280733077868774 1106 70-71 0.06756600850622618 1106 72-73 -0.012868477093029185 1106 74-75 0.1200267514325688 1106 76-77 0.10241260253370754 1106 78-79 0.12484330845158098 1106 80-81 0.11541794710082343 1106 82-83 -0.04691727413378288 1106 84-85 -0.06263145560420114 1106 86-87 0.02872400704390543 1106 88-89 -0.09101454389356434 1106 90-91 0.08869073779746728 1106 92-93 0.10860393922984457 1106 94-95 -0.13809020429691188 1106 96-97 -0.01483921647999864 1106 98-99 -0.07864493888101975 1106 100-101 0.039153487887574556 1107 1 -0.1409377629180426 1107 2 -0.12496515920459927 1107 3 -0.13545038574334 1107 4 -0.08220859871546082 1107 5 -0.16743978569571993 1107 6 -0.15326729047728804 1107 7 -0.1522172235157342 1107 8 -0.10669763733847049 1107 9 -0.131935206617662 1107 10-11 -0.12134227508101247 1107 12-13 -0.1883074277562855 1107 14-15 -0.08177381267592665 1107 16-17 -0.1715933718648941 1107 18-19 -0.2176311591582163 1107 20-21 -0.17115662565190348 1107 22-23 -0.2064676419696525 1107 24-25 -0.13702695368750284 1107 26-27 -0.1419873093070123 1107 28-29 -0.37296769322451695 1107 30-31 -0.13933737077476138 1107 32-33 -0.14401731031748 1107 34-35 -0.07646690608175533 1107 36-37 -0.18424551219339946 1107 38-39 -0.20164122125311934 1107 40-41 -0.3160931357955832 1107 42-43 -0.30175204141688283 1107 44-45 -0.30370056609079654 1107 46-47 -0.1905899833066087 1107 48-49 -0.22705638628243463 1107 50-51 -0.13528116022047243 1107 52-53 -0.11412215385004032 1107 54-55 -0.1983690648995946 1107 56-57 -0.16967143390133543 1107 58-59 -0.23122183562865928 1107 60-61 -0.29027926269061055 1107 62-63 -0.2958758520978577 1107 64-65 -0.3340431124879828 1107 66-67 -0.40509642821184144 1107 68-69 -0.4568409287546302 1107 70-71 -0.7213985235904588 1107 72-73 -0.35941407446320284 1107 74-75 -0.29306532631643023 1107 76-77 -0.4137228067511387 1107 78-79 -0.4099982162255884 1107 80-81 -0.4660890555083661 1107 82-83 -0.4784698074379108 1107 84-85 -0.48641800885731 1107 86-87 -0.42309227068982835 1107 88-89 -0.3260098037916883 1107 90-91 -0.36546357956176934 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2316 52-53 0.033549343407784704 2316 54-55 -0.04859287268790524 2316 56-57 0.032928155554778016 2316 58-59 -0.03421838019671242 2316 60-61 0.04980180657708644 2316 62-63 0.142325582426146 2316 64-65 0.06360208391055266 2316 66-67 0.11389279219311987 2316 68-69 0.19043856045762908 2316 70-71 0.13540143833591145 2316 72-73 0.10426944854941667 2316 74-75 0.19530750214060077 2316 76-77 0.2128184549136094 2316 78-79 0.07704485331012734 2316 80-81 -0.2154237063895117 2316 82-83 -0.04498843783945006 2316 84-85 -0.05767642982886301 2316 86-87 0.05091340027640712 2316 88-89 -0.010132413387996309 2316 90-91 -0.08916835617354124 2316 92-93 -0.04536881590423647 2316 94-95 0.011110736814998745 2316 96-97 -0.04564307722031913 2316 98-99 0.17964017354495354 2316 100-101 0.07428753711018032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 9.0 4 28.0 5 50.0 6 104.0 7 318.0 8 719.0 9 1460.0 10 2442.0 11 3790.0 12 5699.0 13 7474.0 14 9864.0 15 12837.0 16 16690.0 17 21534.0 18 19127.0 19 13679.0 20 12499.0 21 12740.0 22 13878.0 23 15471.0 24 17856.0 25 21521.0 26 26706.0 27 33858.0 28 42386.0 29 52917.0 30 65681.0 31 83352.0 32 106022.0 33 137926.0 34 187099.0 35 267084.0 36 409389.0 37 609771.0 38 466793.0 39 41973.0 40 7.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.97485258398716 19.532795089369476 15.446102719959583 23.046249606683784 2 16.027409905497052 20.47679458369409 38.934587875411246 24.561207635397615 3 19.587078187408533 23.687873550448217 29.64063630502623 27.084411957117023 4 13.595061223894103 17.068915191406763 36.26778703717217 33.068236547526965 5 14.196655532063499 37.0129462568685 34.06612012964502 14.724278081422973 6 32.84737341198302 36.10617635029103 16.73166686186793 14.31478337585802 7 29.122787430206426 32.150267754101876 20.193406137930307 18.533538677761396 8 26.721009237945225 34.88272052907933 19.281834497790943 19.1144357351845 9 26.059441155212 16.804748754086912 19.934062109027384 37.201747981673705 10-11 25.142925632698365 26.56395431779714 26.906249760559202 21.386870288945296 12-13 26.044942102383427 23.55697472478814 27.530727634994246 22.867355537834186 14-15 22.839222391153406 25.26106800264598 25.594825670680514 26.304883935520095 16-17 22.46345084449173 28.35104921589296 26.43577823823383 22.74972170138148 18-19 22.525532142350247 26.954703456448776 28.594813630228977 21.924950770972004 20-21 22.993832734776948 26.03380958792102 28.910911969339903 22.061445707962132 22-23 22.811016359181004 25.85897011154717 28.772889937111508 22.55712359216032 24-25 22.81341290037634 26.088794324326248 28.822115994183346 22.275676781114072 26-27 22.48125797127995 26.314258938004308 28.76748390966755 22.43699918104819 28-29 22.444132763453347 26.62803654840708 28.64814532377566 22.279685364363917 30-31 22.51036082129109 26.270795755953653 28.631915507290294 22.586927915464965 32-33 22.428825424763417 26.213741365992878 28.639162626730315 22.718270582513398 34-35 22.99573001804973 26.300213699771707 28.41318524307665 22.290871039101916 36-37 23.042997831436562 26.020462613488366 28.456598356401496 22.47994119867358 38-39 22.618585675711817 26.379872958155076 28.42693999326097 22.574601372872138 40-41 22.918756643915675 26.319200936820614 28.26087075498598 22.50117166427773 42-43 22.78034720997006 26.274249934910216 28.141227402624864 22.80417545249486 44-45 22.78250456716219 26.47768265821062 28.23372778582299 22.506084988804204 46-47 23.08639826675876 26.799545819573467 27.644457141647887 22.46959877201989 48-49 23.081079700521023 26.74506341306791 27.501842554619888 22.672014331791182 50-51 23.078995775077757 26.98690746601172 27.445776455111638 22.488320303798893 52-53 23.24806061686276 26.897948394156284 27.233403344442802 22.620587644538155 54-55 23.091798226842837 26.918655074265867 27.307999493571312 22.681547205319983 56-57 23.099898166968956 27.114695517048006 27.23774528315311 22.547661032829932 58-59 23.13778910309947 26.722979493651582 27.3613058562932 22.777925546955753 60-61 23.047558688991852 26.92339828244783 27.439550548187164 22.589492480373153 62-63 22.80308279342854 27.003485528289406 27.618077606912824 22.57535407136923 64-65 22.81289758574352 26.92779487157034 27.50714126478011 22.752166277906028 66-67 22.886070693504468 26.936770480899284 27.308747461015386 22.868411364580858 68-69 22.877697106752468 26.818190276350258 27.50144941496176 22.802663201935523 70-71 22.977870014933806 26.751347527201386 27.322137902575232 22.948644555289572 72-73 22.93689599348502 26.789876972125626 27.33893980540441 22.934287228984942 74-75 22.87357416425584 27.249384386307305 26.983162705540543 22.893878743896312 76-77 23.106921763699937 26.846850199634336 27.23482638581036 22.811401650855366 78-79 23.017676477445498 26.956400065529245 27.203046890991224 22.82287656603403 80-81 23.01915416922526 27.033860303762346 27.019940812059133 22.927044714953258 82-83 23.02671233776374 27.048121029380212 27.04343254197017 22.88173409088587 84-85 22.94003648989869 27.144055194939646 27.0497017522704 22.866206562891257 86-87 23.073956466835305 27.135565633048632 26.91144904645097 22.879028853665094 88-89 22.962144455499768 27.10044764939373 26.769499420051584 23.167908475054922 90-91 22.96581842622782 27.00270072983379 26.928427466621272 23.103053377317117 92-93 22.947084245279182 26.79784456958254 27.142755761244686 23.11231542389359 94-95 22.80342941248799 26.900120076139437 27.076129585906976 23.22032092546559 96-97 22.817093500673717 26.787395909384294 27.088626115155606 23.306884474786383 98-99 22.641094310983974 27.004363816807263 26.832346974517645 23.52219489769112 100-101 22.5730952327086 26.817154454753318 27.128579591938013 23.481170720600073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 249.0 1 180.0 2 163.0 3 213.5 4 205.5 5 231.0 6 371.5 7 776.5 8 1361.0 9 1788.5 10 1776.5 11 1681.0 12 1854.5 13 2055.5 14 2271.0 15 2445.5 16 2576.0 17 2796.0 18 3093.5 19 3450.0 20 3856.0 21 4441.0 22 5339.5 23 6611.5 24 8265.0 25 10565.0 26 13649.5 27 17019.5 28 20171.5 29 23972.0 30 28281.5 31 33193.0 32 39479.5 33 43879.5 34 47374.0 35 52005.0 36 58382.5 37 65251.5 38 70256.0 39 74573.5 40 78270.5 41 83187.0 42 86749.0 43 89319.5 44 94019.5 45 97918.5 46 100755.0 47 105500.5 48 109162.5 49 109133.0 50 106893.5 51 103001.5 52 101357.0 53 102399.5 54 103185.0 55 96727.0 56 85923.0 57 76931.0 58 69811.5 59 61763.5 60 50378.0 61 41316.5 62 34018.5 63 25549.0 64 18193.5 65 13148.0 66 9661.0 67 7667.0 68 6010.0 69 4561.0 70 3475.5 71 2413.5 72 1751.5 73 1492.5 74 1107.0 75 730.0 76 515.0 77 321.5 78 185.0 79 109.5 80 68.0 81 36.0 82 23.5 83 15.0 84 7.5 85 7.5 86 5.5 87 2.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045060477728630845 2 0.0 3 0.0 4 3.648621678431647E-5 5 0.012952606958432348 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006950624297412288 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013499900210197095 24-25 4.1959149301963947E-4 26-27 0.0022803885490197797 28-29 0.0024445765245492035 30-31 0.0010033709615687029 32-33 0.0 34-35 0.0 36-37 2.3716040909805707E-4 38-39 3.83105276235323E-4 40-41 5.4729325176474706E-5 42-43 0.011511401395451848 44-45 0.0 46-47 0.0 48-49 3.648621678431647E-5 50-51 1.8243108392158237E-5 52-53 2.006741923137406E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8243108392158237E-4 84-85 9.121554196079119E-5 86-87 0.0 88-89 0.0 90-91 0.007497917549177035 92-93 0.002024985031529564 94-95 0.0 96-97 0.0 98-99 0.0014959348881569755 100-101 0.01076343395137336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2740761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.1598997311165 #Duplication Level Percentage of deduplicated Percentage of total 1 73.45398318254875 38.31352397652861 2 14.303201866012587 14.921071503302699 3 5.136000998452895 8.036798912946518 4 2.3264595516119457 4.853915877623093 5 1.317004379413812 3.434740818783287 6 0.7702145458447868 2.410458808962691 7 0.5139233328860614 1.8764332658972718 8 0.3734737304439063 1.5584281865728147 9 0.27105457151834705 1.2724361344851953 >10 1.3858101162357541 13.299977867924628 >50 0.1000402704876553 3.5876050502151897 >100 0.04512578589859312 4.173168212753248 >500 0.002518416437972309 0.8875216016483553 >1k 0.0010493401824640943 0.847894563052549 >5k 1.399120243285459E-4 0.5260252193039353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7792 0.28430060118339395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6624 0.2416846999793123 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4093 0.14933808529820733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 3.648621678431647E-5 10-11 0.0 0.0 0.0 0.0 3.648621678431647E-5 12-13 0.0 0.0 0.0 0.0 7.297243356863295E-5 14-15 0.0 0.0 0.0 0.0 9.121554196079117E-5 16-17 0.0 0.0 0.0 0.0 1.2770175874510765E-4 18-19 0.0 0.0 0.0 0.0 2.1891730070589883E-4 20-21 0.0 0.0 0.0 1.8243108392158237E-5 2.554035174902153E-4 22-23 0.0 0.0 0.0 1.2770175874510765E-4 2.7364662588237355E-4 24-25 0.0 0.0 0.0 2.7364662588237355E-4 3.2837595105884827E-4 26-27 0.0 0.0 0.0 3.83105276235323E-4 4.013483846274812E-4 28-29 0.0 0.0 0.0 8.756692028235953E-4 4.013483846274812E-4 30-31 0.0 0.0 0.0 0.0025175489581178365 4.195914930196394E-4 32-33 0.0 0.0 0.0 0.004724965073568984 4.3783460141179765E-4 34-35 1.8243108392158237E-5 0.0 0.0 0.0074067020072162435 4.3783460141179765E-4 36-37 3.648621678431647E-5 0.0 0.0 0.010289113133177246 4.3783460141179765E-4 38-39 1.0945865035294941E-4 3.648621678431647E-5 0.0 0.012861391416471557 4.7432081819611413E-4 40-41 1.0945865035294941E-4 5.4729325176474706E-5 0.0 0.015470155916550184 4.7432081819611413E-4 42-43 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.01851675501804061 4.925639265882724E-4 44-45 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.02169105587827614 5.108070349804306E-4 46-47 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.025795755266511747 5.108070349804306E-4 48-49 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.03152409130164943 5.472932517647471E-4 50-51 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.03754431707106165 5.472932517647471E-4 52-53 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.04931112198400371 6.2026568533338E-4 54-55 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.07100217786227986 6.2026568533338E-4 56-57 1.0945865035294941E-4 7.297243356863295E-5 0.0 0.10878365534243956 6.2026568533338E-4 58-59 1.0945865035294941E-4 9.121554196079117E-5 0.0 0.1494840301653446 6.2026568533338E-4 60-61 1.0945865035294941E-4 1.0945865035294941E-4 0.0 0.18954589619452406 6.567519021176965E-4 62-63 1.0945865035294941E-4 1.0945865035294941E-4 0.0 0.24035295306668478 6.567519021176965E-4 64-65 1.0945865035294941E-4 1.459448671372659E-4 0.0 0.30836326115265067 6.567519021176965E-4 66-67 1.0945865035294941E-4 1.459448671372659E-4 0.0 0.4106341267990897 6.93238118902013E-4 68-69 1.0945865035294941E-4 1.459448671372659E-4 0.0 0.5373872439078051 7.297243356863295E-4 70-71 1.0945865035294941E-4 1.459448671372659E-4 0.0 0.6459154957327545 7.479674440784877E-4 72-73 1.0945865035294941E-4 1.459448671372659E-4 0.0 0.7625254445754299 8.574260944314371E-4 74-75 1.2770175874510765E-4 1.459448671372659E-4 0.0 0.8934197472891653 8.756692028235953E-4 76-77 1.459448671372659E-4 1.459448671372659E-4 0.0 1.0490517049826673 8.756692028235953E-4 78-79 1.459448671372659E-4 1.459448671372659E-4 0.0 1.1929351008716194 8.756692028235953E-4 80-81 1.459448671372659E-4 1.459448671372659E-4 0.0 1.3524345975442587 8.756692028235953E-4 82-83 1.459448671372659E-4 1.459448671372659E-4 0.0 1.539462944780665 8.756692028235953E-4 84-85 1.459448671372659E-4 1.459448671372659E-4 0.0 1.7877881362147228 8.756692028235953E-4 86-87 1.459448671372659E-4 1.459448671372659E-4 0.0 2.0547942706423505 8.939123112157535E-4 88-89 1.459448671372659E-4 1.459448671372659E-4 0.0 2.4054815432648082 9.121554196079118E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6925 0.0 37.744965 1 GTATCAA 9985 0.0 31.508375 1 ATCAACG 12360 0.0 25.24974 3 TCAACGC 12390 0.0 25.226944 4 TATCAAC 12540 0.0 24.925186 2 CAACGCA 12650 0.0 24.708445 5 AACGCAG 13090 0.0 23.948301 6 GTATAGG 810 0.0 21.70865 1 GTACATG 8410 0.0 21.530054 1 TAAGGTG 1550 0.0 21.452425 5 TACATGG 8200 0.0 21.375809 2 GTAAGGT 1290 0.0 21.357397 4 GAGTACT 8975 0.0 20.775661 12-13 ACGCAGA 14985 0.0 20.76511 7 CGCAGAG 14960 0.0 20.672789 8 ACATGGG 8210 0.0 20.019028 3 GTACTTT 9220 0.0 19.988087 14-15 AGAGTAC 12695 0.0 19.851913 10-11 AGTACTT 9315 0.0 18.920853 12-13 GCAGAGT 16435 0.0 18.644024 9 >>END_MODULE