FastQCFastQC Report
Thu 26 May 2016
SRR937505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937398
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73500.2502214544981647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57410.19544508439101543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44300.15081374740501627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36440.1240553714546003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98900.038.3680761
GGTATCA60950.036.9800951
ATCAACG120350.031.1218593
TCAACGC121200.030.8256864
TATCAAC123750.030.3830622
CAACGCA123650.030.2517995
AACGCAG128350.029.2751546
ACGCAGA146650.025.3952567
CGCAGAG146250.025.3668388
GCAGAGT159300.022.6311959
GTACATG95650.022.3215731
TACATGG90800.022.1439342
AGAGTAC128600.021.88411110-11
GAGTACT84550.021.60015312-13
ACATGGG93700.020.9505713
AGTACTT88400.020.60569212-13
GTACTTT89150.020.27198614-15
TAAGGTG14100.019.8885655
CAGAGTA153350.018.59990510-11
TACTTTT101300.017.88743614-15
Produced by FastQC (version 0.11.5)