##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937505_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2937398 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28566574907452 31.0 31.0 34.0 28.0 34.0 2 31.38003702596652 31.0 31.0 34.0 28.0 34.0 3 31.42452163445335 33.0 31.0 34.0 28.0 34.0 4 35.06541946307583 37.0 35.0 37.0 32.0 37.0 5 35.0378307604213 37.0 35.0 37.0 32.0 37.0 6 35.086588538563724 37.0 35.0 37.0 32.0 37.0 7 35.00640703098456 37.0 35.0 37.0 32.0 37.0 8 34.97561174890158 37.0 35.0 37.0 32.0 37.0 9 36.5427116107521 39.0 37.0 39.0 32.0 39.0 10-11 36.42891702111869 39.0 37.0 39.0 32.0 39.0 12-13 36.296653705081845 39.0 37.0 39.0 32.0 39.0 14-15 37.38057593829641 40.0 37.0 41.0 32.0 41.0 16-17 37.30253680298006 40.0 37.0 41.0 32.0 41.0 18-19 37.22755275247005 40.0 37.0 41.0 31.0 41.0 20-21 37.133873244279464 40.0 37.0 41.0 31.0 41.0 22-23 36.982857447305406 39.0 36.0 41.0 31.0 41.0 24-25 36.82287742416928 39.0 36.0 41.0 30.0 41.0 26-27 36.63371255784882 39.0 36.0 41.0 30.0 41.0 28-29 36.436977726545734 39.0 36.0 40.0 30.0 41.0 30-31 36.434151756077995 39.0 36.0 40.0 30.0 41.0 32-33 36.27832932411611 39.0 35.0 40.0 30.0 41.0 34-35 36.057727961958165 39.0 35.0 40.0 29.0 41.0 36-37 36.06310840410458 39.0 35.0 40.0 29.0 41.0 38-39 36.025516630705134 39.0 35.0 41.0 29.0 41.0 40-41 35.85309770756295 39.0 35.0 40.5 28.0 41.0 42-43 35.6066699167086 39.0 35.0 40.0 27.0 41.0 44-45 35.400205726292455 38.5 35.0 40.0 26.5 41.0 46-47 35.11187588471157 38.0 34.5 40.0 25.5 41.0 48-49 34.814086310401244 38.0 34.0 40.0 24.0 41.0 50-51 33.88900703956359 37.5 33.0 39.5 23.5 40.5 52-53 34.02198731666597 38.0 33.5 39.5 23.0 40.5 54-55 34.35769412248527 38.0 34.0 40.0 23.0 41.0 56-57 34.09895730847505 38.0 33.5 40.0 22.5 41.0 58-59 33.826483166394205 38.0 33.0 40.0 22.0 41.0 60-61 33.511826793645255 37.0 33.0 40.0 20.5 41.0 62-63 33.16749092223798 37.0 32.5 40.0 19.5 41.0 64-65 32.785712048554544 36.0 32.0 39.0 18.0 41.0 66-67 32.37402932799709 36.0 31.5 39.0 16.5 40.5 68-69 31.85556128246836 35.0 31.0 39.0 12.5 40.0 70-71 31.406539052590084 35.0 31.0 38.0 8.0 40.0 72-73 30.97952882108587 35.0 30.0 37.0 7.5 39.5 74-75 30.507471748806257 35.0 30.0 37.0 6.5 39.0 76-77 30.058013248460032 34.0 29.0 36.0 2.0 39.0 78-79 29.57336169630401 34.0 29.0 36.0 2.0 38.0 80-81 29.120321284347575 34.0 29.0 35.0 2.0 37.0 82-83 28.66160935630786 34.0 28.5 35.0 2.0 37.0 84-85 28.181018540899125 34.0 27.0 35.0 2.0 36.0 86-87 27.722164820701856 33.0 26.0 35.0 2.0 36.0 88-89 27.306628689745143 33.0 26.0 35.0 2.0 35.5 90-91 26.83766125666321 33.0 25.0 35.0 2.0 35.0 92-93 26.352417683950215 32.5 24.5 35.0 2.0 35.0 94-95 25.76205539732784 32.0 23.5 34.5 2.0 35.0 96-97 25.072733759606294 32.0 19.0 34.0 2.0 35.0 98-99 24.293047452200895 31.5 8.5 34.0 2.0 35.0 100-101 22.440861435869433 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14685960212267446 1101 2 0.18901113587297402 1101 3 0.3078353716058828 1101 4 0.20594473382764988 1101 5 0.2031095116831736 1101 6 0.11141780514815736 1101 7 0.27052550486466487 1101 8 0.05858856685562586 1101 9 0.16476675410763164 1101 10-11 0.15719977852914013 1101 12-13 0.25183728640656255 1101 14-15 0.4559971764572808 1101 16-17 0.3781653290711091 1101 18-19 0.5295271529848122 1101 20-21 0.5034285288313853 1101 22-23 0.37984586321331193 1101 24-25 0.4376189561955215 1101 26-27 0.47972730169519195 1101 28-29 0.5338317133689259 1101 30-31 0.49573223511078623 1101 32-33 0.491341269478049 1101 34-35 0.4801451786293285 1101 36-37 0.5377557835265847 1101 38-39 0.5887135735874978 1101 40-41 0.6361604811628823 1101 42-43 0.6497182545854159 1101 44-45 0.473382705786058 1101 46-47 0.5356375184664515 1101 48-49 0.6467364051744156 1101 50-51 0.5245926369464584 1101 52-53 0.6641023985358245 1101 54-55 0.7476657984547828 1101 56-57 0.8509386109888268 1101 58-59 0.8033693919659939 1101 60-61 0.7172078213964426 1101 62-63 0.8706076348617344 1101 64-65 0.6417845847572323 1101 66-67 0.7068304760157318 1101 68-69 0.694884016582229 1101 70-71 0.7498470919732547 1101 72-73 0.6227128663310886 1101 74-75 0.6369561464015554 1101 76-77 0.6226965716871433 1101 78-79 0.8068944417642854 1101 80-81 0.9188840186403517 1101 82-83 0.8980351730596503 1101 84-85 0.6810335516172081 1101 86-87 0.9178590784137519 1101 88-89 1.0556922826236423 1101 90-91 1.0791159300538844 1101 92-93 1.0897719889122932 1101 94-95 1.0236086918918907 1101 96-97 0.891722371202615 1101 98-99 0.8757623146307694 1101 100-101 1.242617204695513 1102 1 -0.05549348151232891 1102 2 -0.09875105511935445 1102 3 -0.0444093648619841 1102 4 -0.24563386820524613 1102 5 -0.08813921933847269 1102 6 0.008860699362180924 1102 7 -0.005390447583671687 1102 8 0.03649334807037974 1102 9 -0.011238889237993988 1102 10-11 -0.07953871125638301 1102 12-13 0.05538507532178727 1102 14-15 0.12310821435132624 1102 16-17 0.12797644313906176 1102 18-19 0.07184126261608981 1102 20-21 0.2550112141233498 1102 22-23 0.21601635758334226 1102 24-25 0.20125035336005226 1102 26-27 0.3173577723823158 1102 28-29 0.24425148648052186 1102 30-31 0.19457709520284538 1102 32-33 0.06740436005458861 1102 34-35 0.15191282147651464 1102 36-37 0.23742715157325023 1102 38-39 0.3507310978089748 1102 40-41 0.26155849331972547 1102 42-43 0.24440537133987306 1102 44-45 0.11916744481651875 1102 46-47 0.33974302424849157 1102 48-49 0.4062905977662652 1102 50-51 0.1782228654540816 1102 52-53 0.19486989635690577 1102 54-55 0.35107053250085585 1102 56-57 0.24839234695966894 1102 58-59 0.15714014090611528 1102 60-61 0.2389731410100282 1102 62-63 0.3166952390728426 1102 64-65 0.21097188948240841 1102 66-67 0.3229728662992599 1102 68-69 0.2902697930420999 1102 70-71 0.16545218168527143 1102 72-73 0.21500057558051822 1102 74-75 0.06387299012678227 1102 76-77 0.22689247237665455 1102 78-79 0.2576949717431525 1102 80-81 0.2565411009123899 1102 82-83 0.23466862452589154 1102 84-85 0.3707844305739201 1102 86-87 0.3939127352538456 1102 88-89 0.5407046098419421 1102 90-91 0.4891332909449915 1102 92-93 0.28771853201109465 1102 94-95 0.23814819179122182 1102 96-97 0.5047421947213415 1102 98-99 0.5638919989385229 1102 100-101 0.8213080836803854 1103 1 0.08158814595597619 1103 2 0.04166338858316365 1103 3 0.11644758079089712 1103 4 -0.0556988653162378 1103 5 -0.06955557630773512 1103 6 0.001606922852424475 1103 7 0.12562493274273123 1103 8 0.22846437253366503 1103 9 0.1306009494270839 1103 10-11 0.05564246453708677 1103 12-13 0.04379013929594322 1103 14-15 0.23065628770745406 1103 16-17 0.25608887397706326 1103 18-19 0.11496193331612403 1103 20-21 0.1392994946338817 1103 22-23 0.12485852769096795 1103 24-25 0.08831275823068552 1103 26-27 -0.022878834188439612 1103 28-29 -0.20299383658277037 1103 30-31 -0.07137642433114166 1103 32-33 -0.01691539605448611 1103 34-35 0.04950898505070711 1103 36-37 0.18227785606459435 1103 38-39 0.10890991967731622 1103 40-41 0.09410808424936334 1103 42-43 0.023381470119289816 1103 44-45 -0.09845237042171817 1103 46-47 -0.05705365912272242 1103 48-49 -0.12256623097726305 1103 50-51 -0.2276061905702349 1103 52-53 -0.22730848485305444 1103 54-55 -0.07765454838018826 1103 56-57 0.06302445341913199 1103 58-59 -0.1298482963079337 1103 60-61 -0.061206269901667554 1103 62-63 0.0061952042248591965 1103 64-65 -0.04404363473243933 1103 66-67 0.1506860066871809 1103 68-69 0.18185456694612867 1103 70-71 0.13968535741578592 1103 72-73 0.23459615910438814 1103 74-75 0.25276749965826895 1103 76-77 0.28399123156358996 1103 78-79 0.30287054937659263 1103 80-81 0.1180884863018683 1103 82-83 0.20086563029716586 1103 84-85 0.00868419877862081 1103 86-87 0.19789529777225212 1103 88-89 0.16531245760796054 1103 90-91 0.290129493638851 1103 92-93 0.2813181938732363 1103 94-95 0.28664759263071815 1103 96-97 0.4611628795854017 1103 98-99 0.6990432729133751 1103 100-101 0.9119773673182472 1104 1 -0.046550787980592645 1104 2 -0.03238880245573483 1104 3 0.08943274432258974 1104 4 -0.043606747684975744 1104 5 0.009087260422255383 1104 6 -0.0883346471482298 1104 7 -0.030240103255245288 1104 8 0.02169387813392376 1104 9 0.09474827428341825 1104 10-11 0.05060399611235766 1104 12-13 0.0961540666044769 1104 14-15 0.10345315479900563 1104 16-17 0.03728036220326203 1104 18-19 -0.01577927894280151 1104 20-21 0.05755762129304287 1104 22-23 0.050865773768379086 1104 24-25 -0.002392369974444364 1104 26-27 0.09169479380837942 1104 28-29 0.11855790684859357 1104 30-31 -0.14385863193151494 1104 32-33 -0.09690096931922909 1104 34-35 -0.03241158699172786 1104 36-37 -0.055588622606912 1104 38-39 -0.10868617821789428 1104 40-41 -0.18065300522739136 1104 42-43 -0.1372245474475804 1104 44-45 -0.08501971125419772 1104 46-47 -0.06250069854318241 1104 48-49 -0.12078694797566669 1104 50-51 -0.2495801914585556 1104 52-53 -0.21400933324149207 1104 54-55 -0.12643662548575207 1104 56-57 -0.15667798225450014 1104 58-59 -0.32917253804355084 1104 60-61 -0.2591919929427817 1104 62-63 -0.03708758388805933 1104 64-65 -0.18885022523696904 1104 66-67 -0.11146064159314761 1104 68-69 0.05401578829494724 1104 70-71 -0.05050773358971483 1104 72-73 -0.036671439618899626 1104 74-75 -0.08932641701098021 1104 76-77 -0.14958609091697994 1104 78-79 -0.20812338037656986 1104 80-81 -0.07269062626454215 1104 82-83 0.013502537948845372 1104 84-85 -0.019653404041996225 1104 86-87 -0.014465169185701399 1104 88-89 -0.12111578057433903 1104 90-91 0.2050866868641421 1104 92-93 0.0707290907065854 1104 94-95 0.12076200111735957 1104 96-97 0.03718144496881237 1104 98-99 0.09204350876811418 1104 100-101 0.27517778703639095 1105 1 0.08930294162313501 1105 2 0.09618262611569506 1105 3 0.0033958645357117234 1105 4 0.016999312921086585 1105 5 0.07686947820447187 1105 6 0.02510607879248994 1105 7 0.005134938369799613 1105 8 0.06405776424781351 1105 9 0.10545631624145813 1105 10-11 0.19369527982863843 1105 12-13 0.09167625275166102 1105 14-15 0.1431343069801656 1105 16-17 0.21979056033845268 1105 18-19 0.23805334676481493 1105 20-21 0.016800461785884124 1105 22-23 0.2600857204883198 1105 24-25 0.1478365677544886 1105 26-27 0.18948554472413548 1105 28-29 0.2245508722165539 1105 30-31 0.07175283992996384 1105 32-33 0.0024663300480654016 1105 34-35 0.2332810537009138 1105 36-37 0.21666516527188406 1105 38-39 0.2215127894810749 1105 40-41 0.06788595581156898 1105 42-43 -0.049902494500535965 1105 44-45 -0.017025205759694018 1105 46-47 -0.04116994596243018 1105 48-49 -0.03753686472558826 1105 50-51 -0.08894187144523613 1105 52-53 -0.023487355219515393 1105 54-55 0.09789321134408624 1105 56-57 0.11681415061263323 1105 58-59 0.1936827316867138 1105 60-61 0.2546857959350106 1105 62-63 0.22933453565905637 1105 64-65 0.16214877576203435 1105 66-67 0.23442576366826273 1105 68-69 0.2512477230268786 1105 70-71 0.3187511325441541 1105 72-73 0.29777536357790524 1105 74-75 0.37304142348186176 1105 76-77 0.4019176137117242 1105 78-79 0.433644227016039 1105 80-81 0.4735808106318977 1105 82-83 0.5393360794073878 1105 84-85 0.4152689652553718 1105 86-87 0.24297842888289622 1105 88-89 0.5068052567217016 1105 90-91 0.5362773711798248 1105 92-93 0.4921087527112462 1105 94-95 0.42419814830350333 1105 96-97 0.39383832975069666 1105 98-99 0.4211435753681805 1105 100-101 0.6019811919647964 1106 1 -0.0183912306569205 1106 2 0.012416259801870666 1106 3 -0.1476136344874135 1106 4 -0.08225563706871242 1106 5 -0.07868486233149952 1106 6 -0.012686983931679663 1106 7 -0.02501461839106156 1106 8 -0.0802109276134999 1106 9 -0.06302832348092835 1106 10-11 -0.1861084519825127 1106 12-13 -0.3234874650624704 1106 14-15 -0.4098677634132102 1106 16-17 -0.4178427576856336 1106 18-19 -0.23638686333636372 1106 20-21 -0.2489492903501258 1106 22-23 -0.25473435941135847 1106 24-25 -0.2864269845739713 1106 26-27 -0.07780506487549843 1106 28-29 0.030447392643466742 1106 30-31 0.08644115095552962 1106 32-33 0.15750088520509564 1106 34-35 0.011014483263799946 1106 36-37 -0.0720831762263856 1106 38-39 -0.2498086172199976 1106 40-41 -0.054589476492388656 1106 42-43 -0.047296550867720555 1106 44-45 -0.07046905727457897 1106 46-47 -0.0589649405677406 1106 48-49 0.11717218668432139 1106 50-51 -0.04933256706825517 1106 52-53 0.05853156992555597 1106 54-55 0.2131233603540892 1106 56-57 0.08193777278042091 1106 58-59 -0.08369831182070442 1106 60-61 -0.05297866911616467 1106 62-63 0.24282754079129631 1106 64-65 0.180163020651662 1106 66-67 0.16805899421093784 1106 68-69 0.10198511841369395 1106 70-71 0.04251646001623399 1106 72-73 0.2339112003375945 1106 74-75 0.27045060684289624 1106 76-77 0.21136642615158152 1106 78-79 0.21434081597005417 1106 80-81 0.37420056174728344 1106 82-83 0.07328478978559971 1106 84-85 0.25091754878554084 1106 86-87 0.15246405641621052 1106 88-89 0.25577762353125166 1106 90-91 0.40794660761291723 1106 92-93 0.3723285679796682 1106 94-95 0.3239435409492337 1106 96-97 0.4014562291762367 1106 98-99 0.4587503755897977 1106 100-101 0.46139719887582586 1107 1 -0.05086751841358961 1107 2 -0.16624402619208212 1107 3 -0.10728519898856703 1107 4 -0.1405584332855483 1107 5 -0.12911189665918954 1107 6 -0.055514080259818854 1107 7 -0.21188543675619087 1107 8 -0.03067164317187121 1107 9 -0.1066492457571897 1107 10-11 -0.2194677401633527 1107 12-13 -0.08175268282662529 1107 14-15 -0.07601548108815592 1107 16-17 -0.07289240332250557 1107 18-19 -0.10487080136645943 1107 20-21 -0.07188992811318684 1107 22-23 -0.10594756728315247 1107 24-25 -0.12496645313493104 1107 26-27 -0.08927878827784497 1107 28-29 -0.06344862556748154 1107 30-31 -0.0708895236260716 1107 32-33 -0.17179328971749896 1107 34-35 -0.041773655048345404 1107 36-37 -0.049808510672839645 1107 38-39 -0.006794587398353258 1107 40-41 0.054998852579437596 1107 42-43 -0.0482568743069649 1107 44-45 -0.005638192270616571 1107 46-47 -0.09820916713074013 1107 48-49 -0.16772384093677317 1107 50-51 -0.2108578598614841 1107 52-53 -0.26456793067962536 1107 54-55 -0.06061529353655715 1107 56-57 -0.16908846608786376 1107 58-59 -0.05886670465783794 1107 60-61 -0.10510223786361195 1107 62-63 0.0424099698773901 1107 64-65 -0.1171328310529205 1107 66-67 -0.1682284304390791 1107 68-69 0.0027614468115544355 1107 70-71 0.04525445821481 1107 72-73 0.03243836113413323 1107 74-75 -0.0066955862103910135 1107 76-77 -0.013571995077573007 1107 78-79 0.022562383072312286 1107 80-81 -0.028899851464778692 1107 82-83 -0.004743942061914197 1107 84-85 0.09796818621659042 1107 86-87 0.1248981796042905 1107 88-89 0.11850339386289832 1107 90-91 0.20655002612466689 1107 92-93 0.14931585380335122 1107 94-95 0.11618228104039119 1107 96-97 0.2154507450125358 1107 98-99 0.3853181463273252 1107 100-101 0.7172261646276787 1108 1 -0.24975701918366866 1108 2 -0.22347952036033192 1108 3 -0.21123714309808506 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-0.7800156671643492 2316 60-61 -0.9063721018003577 2316 62-63 -0.8635714190957273 2316 64-65 -0.7710094875432887 2316 66-67 -0.8592015389477012 2316 68-69 -0.6484240948528139 2316 70-71 -0.6636740621099797 2316 72-73 -0.9945752631134468 2316 74-75 -1.1896450706073018 2316 76-77 -1.054726293851303 2316 78-79 -0.8793829118055925 2316 80-81 -0.9447957560964895 2316 82-83 -1.0699914439748461 2316 84-85 -1.2472854844906287 2316 86-87 -1.2558039442358435 2316 88-89 -0.9203725847538742 2316 90-91 -1.218615836103087 2316 92-93 -1.1830605311039726 2316 94-95 -1.3146136899911376 2316 96-97 -1.3811155289114119 2316 98-99 -1.5131416641513553 2316 100-101 -1.5734100450384823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14398.0 3 3624.0 4 3446.0 5 3899.0 6 4251.0 7 5040.0 8 6276.0 9 7783.0 10 9404.0 11 11398.0 12 14126.0 13 16257.0 14 18613.0 15 21031.0 16 24391.0 17 28166.0 18 25602.0 19 21574.0 20 22424.0 21 23908.0 22 25804.0 23 29112.0 24 32437.0 25 37045.0 26 42192.0 27 49392.0 28 58329.0 29 69161.0 30 83057.0 31 101334.0 32 125512.0 33 159619.0 34 209361.0 35 285440.0 36 401133.0 37 518561.0 38 378817.0 39 45472.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94519158319563 18.641235182367964 14.903363046586438 23.51021018784997 2 15.38038156293576 19.703762228574472 40.09726180997242 24.818594398517345 3 19.352826390916213 23.49720954610096 29.259048701913965 27.890915361068856 4 12.88693991259654 16.318647380405825 36.855046083319074 33.93936662367856 5 14.215801244661847 36.653034721006 34.19197862587393 14.939185408458226 6 32.82290395469046 35.52872325218252 17.207545084194578 14.440827708932435 7 29.070990185896132 31.89539401764109 20.443661366331796 18.589954430130977 8 26.494646064318196 34.54864807323823 19.652410431098954 19.304295431344613 9 25.999529175750503 16.921048232191414 20.08986601979509 36.98955657226299 10-11 24.992216173537447 26.310067042417206 27.238308607351037 21.45940817669431 12-13 25.920397738342572 23.403722092241257 27.674373057465086 23.001507111951085 14-15 22.85878427314878 25.065168065549813 25.70891699732573 26.36713066397568 16-17 22.529296873334022 28.116792734249174 26.473390525065412 22.880519867351392 18-19 22.566595843977204 26.85926617322766 28.50277218292177 22.071365799873366 20-21 23.025270862812025 25.957932785594696 28.79257832056954 22.22421803102374 22-23 22.761049864414602 25.773285386748025 28.70964699010995 22.75601775872742 24-25 22.6764912819332 26.02359783892577 28.688831749199405 22.611079129941626 26-27 22.392113299586736 26.27807814303421 28.63214037510649 22.69766818227256 28-29 22.44900222069907 26.536415677746504 28.52330846973739 22.491273631817034 30-31 22.50477548604869 26.198545918450034 28.46962497443151 22.82705362106977 32-33 22.368874716866152 26.16503916062895 28.505191476700926 22.96089464580397 34-35 22.963124322144544 26.308996666460832 28.248494858107094 22.479384153287533 36-37 22.932742906951553 25.971704566195246 28.348466420921014 22.747086105932183 38-39 22.557510915108953 26.310094501527725 28.299170352793652 22.833224230569673 40-41 22.901436759455535 26.203547859119936 28.236546455722717 22.65846892570182 42-43 22.674561897093085 26.141145010812817 28.06205546048584 23.122237631608257 44-45 22.721811527264943 26.388212926783712 28.138276684723596 22.75169886122775 46-47 23.011481195299723 26.64294011390792 27.595805977863176 22.749772712929182 48-49 22.929302199584928 26.61135776118685 27.528280791920174 22.93105924730805 50-51 22.973886827679813 26.808736554278646 27.484818438832587 22.732558179208954 52-53 23.03872132429103 26.746825601426604 27.3167270326891 22.897726041593266 54-55 22.8815798924516 26.769823595577886 27.40544388922641 22.943152622744105 56-57 22.928246759393254 26.944308619110878 27.288959125793856 22.838485495702017 58-59 22.943861803175253 26.585563001995194 27.444774134138232 23.025801060691325 60-61 22.873000653870985 26.761912850628512 27.548041780889438 22.817044714611065 62-63 22.658620473824662 26.793095632506102 27.745022279442725 22.803261614226507 64-65 22.64037581808582 26.733652950156966 27.63110861287299 22.994862618884223 66-67 22.68072905651109 26.796425671849523 27.40513259604429 23.117712675595094 68-69 22.76641436234748 26.663129562719128 27.59046816998701 22.979987904946388 70-71 22.77966760783899 26.58792769545244 27.456677221099884 23.175727475608685 72-73 22.769467791080615 26.595588803462554 27.525347160447666 23.10959624500916 74-75 22.71142309110576 27.059208392076894 27.144675795242545 23.084692721574797 76-77 22.94759411924244 26.72114845006661 27.30775725755692 23.023500173134035 78-79 22.85748009953134 26.77657779485 27.327213716057308 23.038728389561353 80-81 22.829774918144455 26.902921831067346 27.133283919269747 23.134019331518452 82-83 22.977972155603847 26.857424546871854 27.15633731224137 23.00826598528293 84-85 22.92014249688806 26.951193161595555 27.097909223636172 23.030755117880208 86-87 23.01140012064757 26.90972870670208 27.07630557842356 23.00256559422679 88-89 22.954789050363335 26.800247521881605 26.93937624212934 23.30558718562572 90-91 22.92036919667862 26.732221204180444 27.113026060580836 23.234383538560095 92-93 22.934677374786702 26.51588444673083 27.297552874810926 23.25188530367154 94-95 22.826949616455053 26.50825790444748 27.35404730191528 23.310745177182195 96-97 22.85098874199926 26.441565777642545 27.285847611068892 23.421597869289304 98-99 22.702556937917215 26.63226122934083 27.151327694307515 23.51385413843444 100-101 22.885416025374617 26.17694322789625 27.514178913959224 23.423461832769906 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3940.0 1 2201.0 2 456.0 3 416.0 4 349.0 5 326.5 6 459.5 7 867.0 8 1425.5 9 1787.0 10 1798.5 11 1769.5 12 1878.0 13 2061.5 14 2219.0 15 2367.0 16 2569.5 17 2799.0 18 3144.0 19 3473.0 20 3894.5 21 4580.0 22 5456.5 23 6793.5 24 8559.5 25 10897.5 26 14237.5 27 18048.5 28 21484.0 29 25355.0 30 29772.5 31 35348.0 32 42534.5 33 47331.5 34 50964.0 35 55888.5 36 62627.5 37 70047.5 38 75391.5 39 79653.5 40 83807.0 41 88618.5 42 92148.0 43 95353.5 44 99955.5 45 104694.5 46 108150.0 47 112357.0 48 116039.0 49 116241.0 50 113750.0 51 109618.5 52 108081.0 53 109124.5 54 109126.5 55 102866.0 56 92505.0 57 82916.0 58 75048.5 59 66112.5 60 54780.0 61 45335.0 62 37139.5 63 28260.5 64 20617.5 65 15319.0 66 11252.0 67 8624.5 68 6729.5 69 5150.0 70 3926.0 71 2804.0 72 2088.5 73 1686.0 74 1220.0 75 854.5 76 642.0 77 399.5 78 255.0 79 183.5 80 122.0 81 74.5 82 48.0 83 32.5 84 24.5 85 20.0 86 16.5 87 10.5 88 11.5 89 11.5 90 9.0 91 8.5 92 6.0 93 4.5 94 3.0 95 2.0 96 4.0 97 5.0 98 3.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32804543340738984 2 0.29284421110111736 3 0.29492087895477564 4 0.2955336661902813 5 0.2808267725381443 6 0.213488264103128 7 0.2217608917824551 8 0.2202629674289967 9 0.21679050642779765 10-11 0.22610146803395387 12-13 0.22560783387201871 14-15 0.20872214116030582 16-17 0.2211651264146023 18-19 0.20882427236622345 20-21 0.21296058620588698 22-23 0.21173501173487558 24-25 0.20376877767330132 26-27 0.2157351506333156 28-29 0.21580323810392738 30-31 0.2227992257092842 32-33 0.2241609751215191 34-35 0.213760613985575 36-37 0.2104924153962112 38-39 0.20123251939301381 40-41 0.20221978771688415 42-43 0.20165806608433723 44-45 0.2175564904721798 46-47 0.20708804186562393 48-49 0.21600750051576262 50-51 0.22264602890040777 52-53 0.23161655315350524 54-55 0.2286887919172002 56-57 0.19515571264091552 58-59 0.18206589641580745 60-61 0.19156409856614595 62-63 0.19267051996358683 64-65 0.18907890588881726 66-67 0.20215170024627235 68-69 0.1913598361543107 70-71 0.1964493745825387 72-73 0.20945408146938208 74-75 0.1998707699807789 76-77 0.20913066598397628 78-79 0.20630503595358887 80-81 0.19435568486122753 82-83 0.20793913524827073 84-85 0.18875549040341144 86-87 0.19467910034663333 88-89 0.20221978771688415 90-91 0.1989515891275203 92-93 0.2056241612474714 94-95 0.20407517129105418 96-97 0.19724940236222668 98-99 0.20363260273207787 100-101 0.1869511724322002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2937398.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.61641569756759 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06865131931487 40.24912014992141 2 13.438185794865387 14.41014711597301 3 4.839885437337674 7.784919286107014 4 2.1407475044747 4.591168324137838 5 1.196705109287353 3.2081519303476886 6 0.7403388557296787 2.381658950751838 7 0.48733251287396867 1.829031581523364 8 0.3516421342589176 1.5083032677764803 9 0.2687780282305623 1.296982304278415 >10 1.323160828120533 12.877357297956731 >50 0.09784797654741008 3.576322509102087 >100 0.042917142048774296 4.020340072657388 >500 0.0028555176827555987 1.0259917116317494 >1k 8.249273304305148E-4 0.7951129558817575 >5k 1.2691189698930998E-4 0.44539254195327715 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7350 0.2502214544981647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5741 0.19544508439101543 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4430 0.15081374740501627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3644 0.1240553714546003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.404373530587275E-5 7 0.0 0.0 0.0 0.0 3.404373530587275E-5 8 0.0 0.0 0.0 0.0 3.404373530587275E-5 9 0.0 0.0 0.0 3.404373530587275E-5 3.404373530587275E-5 10-11 0.0 0.0 0.0 3.404373530587275E-5 3.404373530587275E-5 12-13 0.0 3.404373530587275E-5 0.0 3.404373530587275E-5 1.0213120591761825E-4 14-15 0.0 3.404373530587275E-5 0.0 3.404373530587275E-5 1.1915307357055461E-4 16-17 0.0 3.404373530587275E-5 0.0 3.404373530587275E-5 1.36174941223491E-4 18-19 0.0 3.404373530587275E-5 0.0 3.404373530587275E-5 1.36174941223491E-4 20-21 0.0 3.404373530587275E-5 0.0 3.404373530587275E-5 2.72349882446982E-4 22-23 0.0 3.404373530587275E-5 0.0 5.106560295880912E-5 3.0639361775285475E-4 24-25 0.0 3.404373530587275E-5 0.0 1.1915307357055461E-4 3.404373530587275E-4 26-27 0.0 3.404373530587275E-5 0.0 2.5532801479404563E-4 3.5745922071166386E-4 28-29 0.0 3.404373530587275E-5 0.0 9.191808532585642E-4 3.744810883646002E-4 30-31 0.0 3.404373530587275E-5 0.0 0.002587323883246329 3.744810883646002E-4 32-33 0.0 3.404373530587275E-5 0.0 0.004936341619351549 3.744810883646002E-4 34-35 0.0 3.404373530587275E-5 0.0 0.007472599899639068 4.08524823670473E-4 36-37 0.0 3.404373530587275E-5 0.0 0.010059923782885397 4.08524823670473E-4 38-39 0.0 3.404373530587275E-5 0.0 0.012902575680925772 4.08524823670473E-4 40-41 0.0 3.404373530587275E-5 0.0 0.015387768358254483 4.08524823670473E-4 42-43 0.0 3.404373530587275E-5 0.0 0.018672988815271203 4.08524823670473E-4 44-45 0.0 3.404373530587275E-5 0.0 0.02209438421351141 4.4256855897634574E-4 46-47 0.0 3.404373530587275E-5 0.0 0.027677556803674543 4.766122942822185E-4 48-49 0.0 3.404373530587275E-5 0.0 0.03448630386484909 4.936341619351549E-4 50-51 0.0 3.404373530587275E-5 0.0 0.040580132484600316 5.106560295880913E-4 52-53 0.0 3.404373530587275E-5 0.0 0.05275076785644982 5.617216325469003E-4 54-55 1.7021867652936374E-5 3.404373530587275E-5 0.0 0.07601966093801385 5.787435001998367E-4 56-57 3.404373530587275E-5 3.404373530587275E-5 0.0 0.11486356292201465 5.95765367852773E-4 58-59 3.404373530587275E-5 3.404373530587275E-5 0.0 0.15740121018670267 6.468309708115822E-4 60-61 3.404373530587275E-5 3.404373530587275E-5 0.0 0.1995473544953731 6.468309708115822E-4 62-63 3.404373530587275E-5 3.404373530587275E-5 0.0 0.252008750601723 6.468309708115822E-4 64-65 3.404373530587275E-5 3.404373530587275E-5 0.0 0.3182238157716455 6.468309708115822E-4 66-67 3.404373530587275E-5 3.404373530587275E-5 0.0 0.420559284101099 6.468309708115822E-4 68-69 3.404373530587275E-5 3.404373530587275E-5 0.0 0.5469806951594575 6.468309708115822E-4 70-71 3.404373530587275E-5 3.404373530587275E-5 0.0 0.6638358165968656 6.80874706117455E-4 72-73 3.404373530587275E-5 3.404373530587275E-5 0.0 0.7831250651086438 8.17049647340946E-4 74-75 3.404373530587275E-5 3.404373530587275E-5 0.0 0.9106018319614843 8.340715149938823E-4 76-77 3.404373530587275E-5 3.404373530587275E-5 0.0 1.058623993071419 8.510933826468187E-4 78-79 3.404373530587275E-5 3.404373530587275E-5 0.0 1.1989182262669207 8.510933826468187E-4 80-81 3.404373530587275E-5 3.404373530587275E-5 0.0 1.35288101918773 8.681152502997551E-4 82-83 3.404373530587275E-5 3.404373530587275E-5 0.0 1.5307254924256093 9.53224588564437E-4 84-85 3.404373530587275E-5 3.404373530587275E-5 0.0 1.765984725256843 9.53224588564437E-4 86-87 5.106560295880912E-5 3.404373530587275E-5 0.0 2.0238319764635233 9.53224588564437E-4 88-89 6.80874706117455E-5 3.404373530587275E-5 0.0 2.3564903360048586 9.53224588564437E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9890 0.0 38.368076 1 GGTATCA 6095 0.0 36.980095 1 ATCAACG 12035 0.0 31.121859 3 TCAACGC 12120 0.0 30.825686 4 TATCAAC 12375 0.0 30.383062 2 CAACGCA 12365 0.0 30.251799 5 AACGCAG 12835 0.0 29.275154 6 ACGCAGA 14665 0.0 25.395256 7 CGCAGAG 14625 0.0 25.366838 8 GCAGAGT 15930 0.0 22.631195 9 GTACATG 9565 0.0 22.321573 1 TACATGG 9080 0.0 22.143934 2 AGAGTAC 12860 0.0 21.884111 10-11 GAGTACT 8455 0.0 21.600153 12-13 ACATGGG 9370 0.0 20.950571 3 AGTACTT 8840 0.0 20.605692 12-13 GTACTTT 8915 0.0 20.271986 14-15 TAAGGTG 1410 0.0 19.888565 5 CAGAGTA 15335 0.0 18.599905 10-11 TACTTTT 10130 0.0 17.887436 14-15 >>END_MODULE