FastQCFastQC Report
Thu 26 May 2016
SRR937505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937398
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83570.2845034959511786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.23541242964011005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.14945199799278136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68800.036.5530171
GTATCAA106500.034.639221
ATCAACG128950.028.291483
TCAACGC129100.028.2218134
TATCAAC132150.027.6423512
CAACGCA131850.027.633195
AACGCAG136550.026.679346
TAAGGTG14950.023.1951645
ACGCAGA156250.023.165597
CGCAGAG155900.023.1566588
GTACATG91400.021.9493831
GAGTACT92850.021.87069512-13
TACATGG89400.021.7320942
GTAAGGT13650.021.5761934
ACATGGG87850.021.1963023
AGAGTAC136650.020.89167410-11
GCAGAGT172700.020.683979
GTACTTT98000.020.64690614-15
AGTACTT97850.020.46186312-13
GGTAAGG14950.018.7467773
Produced by FastQC (version 0.11.5)