##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937505_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2937398 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.990503159599072 31.0 31.0 34.0 30.0 34.0 2 32.22360606223603 33.0 31.0 34.0 30.0 34.0 3 32.307890861231606 34.0 31.0 34.0 30.0 34.0 4 35.95218965901114 37.0 35.0 37.0 35.0 37.0 5 35.815537765056014 37.0 35.0 37.0 35.0 37.0 6 35.88809756117489 37.0 35.0 37.0 35.0 37.0 7 35.83645423602794 37.0 35.0 37.0 35.0 37.0 8 35.80805665422255 37.0 35.0 37.0 33.0 37.0 9 37.47989581255247 39.0 37.0 39.0 35.0 39.0 10-11 37.400089977592415 39.0 37.0 39.0 34.0 39.0 12-13 37.314852975320335 39.0 37.0 39.0 34.0 39.0 14-15 38.50481616723372 40.0 38.0 41.0 34.0 41.0 16-17 38.468862578377184 40.0 38.0 41.0 34.0 41.0 18-19 38.44780431524771 40.0 38.0 41.0 34.0 41.0 20-21 38.31829207346094 40.0 38.0 41.0 34.0 41.0 22-23 38.188779321018124 40.0 38.0 41.0 33.0 41.0 24-25 38.0431837973608 40.0 37.0 41.0 33.0 41.0 26-27 37.98386718449458 40.0 37.0 41.0 33.0 41.0 28-29 37.88445692412128 40.0 37.0 41.0 33.0 41.0 30-31 37.989333927509996 40.0 37.0 41.0 33.0 41.0 32-33 37.935712150685745 40.0 37.0 41.0 33.0 41.0 34-35 37.826648108291764 40.0 37.0 41.0 33.0 41.0 36-37 37.665321995861646 40.0 37.0 41.0 33.0 41.0 38-39 37.52930518778865 40.0 37.0 41.0 32.5 41.0 40-41 37.367023637927176 40.0 37.0 41.0 32.0 41.0 42-43 37.169217280055335 40.0 36.0 41.0 31.5 41.0 44-45 36.91381845429186 39.0 36.0 41.0 31.0 41.0 46-47 36.62927955285596 39.0 36.0 41.0 30.5 41.0 48-49 36.36483939186995 39.0 35.0 41.0 30.0 41.0 50-51 36.14502869546449 39.0 35.0 40.0 29.5 41.0 52-53 35.8991733500193 39.0 35.0 40.0 29.0 41.0 54-55 35.65331749391809 38.5 35.0 40.0 28.0 41.0 56-57 35.37841926766478 38.0 34.5 40.0 28.0 41.0 58-59 35.110881467203285 38.0 34.0 40.0 27.5 41.0 60-61 34.76571560952925 37.5 34.0 40.0 26.5 41.0 62-63 34.375115663590705 37.0 33.5 40.0 26.0 41.0 64-65 33.99889238707183 36.5 33.0 39.0 26.0 41.0 66-67 33.54769374119544 36.0 33.0 39.0 25.5 40.0 68-69 33.12726586591262 35.5 32.0 39.0 24.5 40.0 70-71 32.710614632405964 35.0 32.0 38.0 23.5 40.0 72-73 32.24944695951996 35.0 31.0 37.0 22.5 39.5 74-75 31.797726593400007 35.0 31.0 37.0 22.0 39.0 76-77 30.493379514795066 33.5 29.5 35.5 20.0 37.5 78-79 31.15038394524678 34.0 31.0 36.0 21.0 38.0 80-81 31.02778377325783 34.0 31.0 35.5 20.0 37.0 82-83 30.695231119514617 34.0 31.0 35.0 19.5 37.0 84-85 30.321584102665014 34.0 30.0 35.0 18.0 36.0 86-87 29.93291716001713 34.0 30.0 35.0 16.0 36.0 88-89 29.51739583808527 34.0 29.5 35.0 9.0 35.5 90-91 29.043270098229794 34.0 29.0 35.0 4.5 35.0 92-93 28.644942735032842 33.5 29.0 35.0 2.0 35.0 94-95 28.177675956748114 33.0 28.5 35.0 2.0 35.0 96-97 27.628481567700394 33.0 27.0 35.0 2.0 35.0 98-99 27.042137122718813 33.0 26.5 35.0 2.0 35.0 100-101 25.051486553745868 31.5 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2513496342054431 1101 2 0.2095609834809693 1101 3 0.16816591825458005 1101 4 0.06642679157266684 1101 5 0.06732134236316512 1101 6 0.04267736254276144 1101 7 0.07637575485247083 1101 8 0.05188152325646911 1101 9 0.18800267426297523 1101 10-11 0.09928825735906344 1101 12-13 0.10874167481046015 1101 14-15 0.12099442589981635 1101 16-17 0.14276002818667166 1101 18-19 0.07175284501187207 1101 20-21 0.1635166154389367 1101 22-23 0.1273429266230366 1101 24-25 0.19785236684064245 1101 26-27 0.1497463755775641 1101 28-29 0.0011559740325566281 1101 30-31 0.1572701872101021 1101 32-33 0.20478623954993225 1101 34-35 0.13804513532692653 1101 36-37 0.13566094112113092 1101 38-39 0.09187157224894804 1101 40-41 0.12667036371518492 1101 42-43 0.19215393907157363 1101 44-45 0.3183419612083114 1101 46-47 0.35774159193272936 1101 48-49 0.4277687666128429 1101 50-51 0.3683431679751408 1101 52-53 0.3416408013014447 1101 54-55 0.39776518601883026 1101 56-57 0.3300398171158321 1101 58-59 0.11054058339315986 1101 60-61 0.24645148105827275 1101 62-63 0.28675489678828114 1101 64-65 0.11731495329012631 1101 66-67 0.20649142977419643 1101 68-69 0.1820581221992299 1101 70-71 0.08545721677184304 1101 72-73 0.1616369910597868 1101 74-75 0.2323001051492959 1101 76-77 0.277245122854584 1101 78-79 0.2231140579066384 1101 80-81 0.1629867424428113 1101 82-83 0.1778140621027937 1101 84-85 0.20616946409648662 1101 86-87 0.1681040007822716 1101 88-89 0.15364644322890086 1101 90-91 0.250948800420133 1101 92-93 0.04007709314012331 1101 94-95 0.143444726454792 1101 96-97 0.22584887190978264 1101 98-99 0.3457611975100896 1101 100-101 0.6986801838961831 1102 1 0.058802004396529384 1102 2 0.11779691700551354 1102 3 0.1138267514928728 1102 4 0.14237653650977933 1102 5 0.003089706116782054 1102 6 0.07495059734282705 1102 7 0.08210893217714954 1102 8 0.012575508334272456 1102 9 0.13012064665733192 1102 10-11 0.08886876585154369 1102 12-13 0.16323713183274435 1102 14-15 0.15811941727569234 1102 16-17 0.07455932333167681 1102 18-19 0.05182837122428907 1102 20-21 0.021898208670307895 1102 22-23 0.07883775144418337 1102 24-25 0.04805758580893382 1102 26-27 0.01766097069427275 1102 28-29 -0.0010185726200901968 1102 30-31 0.10441605952799193 1102 32-33 0.07284121429709245 1102 34-35 0.005824842092600591 1102 36-37 0.04059140603538225 1102 38-39 -0.09790859353137193 1102 40-41 0.04431806857158449 1102 42-43 0.08078459702541352 1102 44-45 0.02968624049196933 1102 46-47 0.036247597843605206 1102 48-49 0.09809614542804468 1102 50-51 0.14083861654930274 1102 52-53 0.14522449473599153 1102 54-55 0.1387412538966757 1102 56-57 0.08201815613053043 1102 58-59 0.1332242138891928 1102 60-61 0.048068170042057545 1102 62-63 -0.027284474277173842 1102 64-65 0.11494652223314006 1102 66-67 0.057097652942609045 1102 68-69 0.15225187988173872 1102 70-71 0.0766511758012669 1102 72-73 0.20701889546365493 1102 74-75 0.3426904506352493 1102 76-77 0.18833708488872247 1102 78-79 0.0974250079402168 1102 80-81 0.13557962602628137 1102 82-83 0.2852253180992008 1102 84-85 0.1862417518620596 1102 86-87 0.22286515124755013 1102 88-89 0.29374897527192445 1102 90-91 0.34716868814458834 1102 92-93 0.39465154010492753 1102 94-95 0.44094267426510925 1102 96-97 0.3393101098131268 1102 98-99 0.5130261320301024 1102 100-101 0.8047421339762302 1103 1 0.05809020621806482 1103 2 0.09967011980504026 1103 3 0.050900734887243004 1103 4 0.08128419834494593 1103 5 -0.061511261982701626 1103 6 -0.026095966308702145 1103 7 0.06484276777110409 1103 8 -0.06070957740300997 1103 9 0.014842166299224857 1103 10-11 -0.011666094497968515 1103 12-13 0.03287824256165095 1103 14-15 0.016251475397631054 1103 16-17 0.051224663526689085 1103 18-19 0.02663665110530644 1103 20-21 0.09147794198205617 1103 22-23 0.021404901261284692 1103 24-25 0.08247098182366841 1103 26-27 0.05203322360520701 1103 28-29 -0.016149792142158503 1103 30-31 0.042436783961377955 1103 32-33 -0.044516678996131986 1103 34-35 -0.03734911229926041 1103 36-37 0.05631166890159278 1103 38-39 0.03731889635709251 1103 40-41 0.07308047051947852 1103 42-43 0.11498957961266854 1103 44-45 0.02920758656842537 1103 46-47 0.036975583472745654 1103 48-49 0.20153687800011966 1103 50-51 0.10636069555755512 1103 52-53 0.12119827106588588 1103 54-55 0.09953142631550804 1103 56-57 -0.047273710349983844 1103 58-59 0.06312715663283086 1103 60-61 -0.14688651633731808 1103 62-63 -0.17917986737368352 1103 64-65 -0.12639892470912173 1103 66-67 -0.013447481941575745 1103 68-69 0.08871411253587524 1103 70-71 -0.07771190422094776 1103 72-73 0.056553618669489936 1103 74-75 0.061418656593584586 1103 76-77 0.035731917045094264 1103 78-79 0.06841777080081002 1103 80-81 0.09466292170131041 1103 82-83 0.0017299978462261834 1103 84-85 0.07214203266936536 1103 86-87 0.16077076492055298 1103 88-89 0.06221806960654419 1103 90-91 -5.761976327107732E-4 1103 92-93 0.15631358660337824 1103 94-95 0.1336034639878747 1103 96-97 0.08653160135181537 1103 98-99 0.09774877580052177 1103 100-101 0.4181689498372343 1104 1 0.08181719316513991 1104 2 0.04974096230571945 1104 3 0.03478342039474569 1104 4 0.020018605247742016 1104 5 0.0036546282570455446 1104 6 -0.02446159624744837 1104 7 -0.04418056979728391 1104 8 0.05871684796876053 1104 9 0.02989047348194873 1104 10-11 -0.0011709889142395014 1104 12-13 0.015524627442161432 1104 14-15 0.08465998097378957 1104 16-17 0.02885358310538777 1104 18-19 -0.12996147135322644 1104 20-21 -0.15028702618616308 1104 22-23 -0.18021177969438895 1104 24-25 -0.08517250221567707 1104 26-27 -0.12671122864368556 1104 28-29 -0.14064042337207638 1104 30-31 -0.10862903844861194 1104 32-33 -0.09517801913310109 1104 34-35 -0.05682563528390716 1104 36-37 -0.13309354675916296 1104 38-39 -0.2081503684991759 1104 40-41 -0.14004665346436695 1104 42-43 -0.06478583330529375 1104 44-45 -0.1467085025123609 1104 46-47 -0.2712604382603203 1104 48-49 -0.24767864675085605 1104 50-51 -0.3819732747557367 1104 52-53 -0.2887566496903631 1104 54-55 -0.24603471974916147 1104 56-57 -0.2796280743513151 1104 58-59 -0.3170320367095272 1104 60-61 -0.2932050734572087 1104 62-63 -0.18440973105997216 1104 64-65 -0.40636259102802796 1104 66-67 -0.3098003843321422 1104 68-69 -0.24272648650073592 1104 70-71 -0.36666262607418076 1104 72-73 -0.48015846294658004 1104 74-75 -0.5169436937591634 1104 76-77 -0.4941077533780316 1104 78-79 -0.426579526338287 1104 80-81 -0.35759766852087793 1104 82-83 -0.2252870766597681 1104 84-85 -0.30929714607409764 1104 86-87 -0.3035577979493702 1104 88-89 -0.24085656378566966 1104 90-91 -0.32003881896017816 1104 92-93 -0.48439937473366257 1104 94-95 -0.5356014817310992 1104 96-97 -0.3366538235138208 1104 98-99 -0.1974806747095812 1104 100-101 0.20723518886654446 1105 1 -0.004161411563462281 1105 2 0.008788165102920686 1105 3 0.09544775605908029 1105 4 0.10916279439192778 1105 5 -0.026153064050646435 1105 6 0.012441084405232061 1105 7 0.02330402893731076 1105 8 -0.04145797720606481 1105 9 0.03967027701175141 1105 10-11 -0.05703387602712695 1105 12-13 -0.044411269993730684 1105 14-15 -0.04047946291564841 1105 16-17 -0.06472367297187276 1105 18-19 -0.02918516432691831 1105 20-21 -0.023184961584099995 1105 22-23 0.025360772753167282 1105 24-25 -0.05559062575880347 1105 26-27 -0.07556445874691065 1105 28-29 -0.2047877343943867 1105 30-31 -0.08188598983056039 1105 32-33 -0.034693711773797986 1105 34-35 -0.1126968474051182 1105 36-37 -0.09522412451474338 1105 38-39 -0.08096401318782398 1105 40-41 -0.031419863587579755 1105 42-43 -0.16881721858667476 1105 44-45 -0.2333614329152951 1105 46-47 -0.2275624695623506 1105 48-49 -0.17236958081679177 1105 50-51 -0.18528662806909324 1105 52-53 -0.04636674792545392 1105 54-55 -0.06398760020204008 1105 56-57 -0.12028533375857364 1105 58-59 -0.12390641233390198 1105 60-61 -0.13424320199533923 1105 62-63 -0.13781257821282367 1105 64-65 -0.1545373329527706 1105 66-67 -0.2773432581250148 1105 68-69 -0.16037654477579366 1105 70-71 -0.12725932104587656 1105 72-73 -0.010873581161696677 1105 74-75 -0.020800253865726148 1105 76-77 -0.1496990370943152 1105 78-79 -0.1679818739906338 1105 80-81 -0.15608772013592898 1105 82-83 -0.12124008198777503 1105 84-85 0.026406733379783276 1105 86-87 0.07085112645955505 1105 88-89 0.059731389927286216 1105 90-91 0.19427811410675133 1105 92-93 -0.1274334240177133 1105 94-95 -0.15120983171445346 1105 96-97 -0.03840311588810863 1105 98-99 0.0371971474682411 1105 100-101 0.4067398508712081 1106 1 -0.25698250478948736 1106 2 -0.1286365644337195 1106 3 -0.1771129706198309 1106 4 -0.10665238654878806 1106 5 -0.1832182814419525 1106 6 -0.3217891156355961 1106 7 -0.20895820387988095 1106 8 -0.16023731992906676 1106 9 -0.2107760604332043 1106 10-11 -0.4902252343218265 1106 12-13 -0.45474501157364955 1106 14-15 -0.37403386906267855 1106 16-17 -0.3178908645269942 1106 18-19 -0.29521631499906675 1106 20-21 -0.2558253692474679 1106 22-23 -0.22624095719172743 1106 24-25 -0.15089770373389655 1106 26-27 -0.23113465528848565 1106 28-29 -0.19613135772860346 1106 30-31 -0.181632020361576 1106 32-33 -0.2766830572670358 1106 34-35 -0.45344878657637366 1106 36-37 -0.4083493432182692 1106 38-39 -0.26673179013938864 1106 40-41 -0.48776354159767266 1106 42-43 -0.7236853768113889 1106 44-45 -0.5285829431360369 1106 46-47 -0.5218473819351388 1106 48-49 -0.5661943956477984 1106 50-51 -0.6669126158800083 1106 52-53 -0.6602125947455093 1106 54-55 -0.7710925487338116 1106 56-57 -0.5720559210504419 1106 58-59 -0.6456971203501141 1106 60-61 -0.4970665187072001 1106 62-63 -0.5947059310836593 1106 64-65 -0.7581730968112836 1106 66-67 -0.7363573061814677 1106 68-69 -0.5923747842124172 1106 70-71 -0.5958140370228975 1106 72-73 -0.4947518507794051 1106 74-75 -0.624091559671573 1106 76-77 -0.5428744847075322 1106 78-79 -0.49959856690172444 1106 80-81 -0.5307827198443249 1106 82-83 -0.6721629197389198 1106 84-85 -0.7805744654071454 1106 86-87 -0.5786593421491979 1106 88-89 -0.6587099647223553 1106 90-91 -0.7528719967030355 1106 92-93 -0.8109357058244946 1106 94-95 -1.4317823064177446 1106 96-97 -1.1991499337518121 1106 98-99 -0.8051901473149492 1106 100-101 -0.3494527394533513 1107 1 -0.06285397600588283 1107 2 -0.003707606618348791 1107 3 -0.1086283046348413 1107 4 -0.07897110225167836 1107 5 -0.07894735225012539 1107 6 -0.04465215518111876 1107 7 -0.019361460714620193 1107 8 -0.05776221998174691 1107 9 -0.08599908539832768 1107 10-11 -0.1356105683741191 1107 12-13 -0.18038546541269795 1107 14-15 -0.16408577047202044 1107 16-17 -0.15682369506246374 1107 18-19 -0.08210880333891168 1107 20-21 -0.022959741023271363 1107 22-23 -0.00499313417638092 1107 24-25 0.010864928991367151 1107 26-27 -0.1709229304916562 1107 28-29 -0.1724246184463567 1107 30-31 -0.09600485968931594 1107 32-33 -0.1385535693584643 1107 34-35 -0.060830968406371255 1107 36-37 -0.2515079075988709 1107 38-39 -0.1923180202153958 1107 40-41 -0.14172089977473945 1107 42-43 -0.03278874587547165 1107 44-45 0.01614109714863332 1107 46-47 -0.11084972328669096 1107 48-49 -0.12406654439491405 1107 50-51 -0.1294312386532468 1107 52-53 -0.1546650900950297 1107 54-55 -0.2525343149157635 1107 56-57 -0.2193998976763467 1107 58-59 -0.21568758769183205 1107 60-61 -0.1436544875301422 1107 62-63 -0.26195889736674616 1107 64-65 -0.11028546215516144 1107 66-67 -0.12834863925688467 1107 68-69 -0.2778970217256358 1107 70-71 -0.36974812905095433 1107 72-73 -0.27993759557581654 1107 74-75 -0.30242182464538914 1107 76-77 -0.16255080399707467 1107 78-79 -0.20079789036529405 1107 80-81 -0.16507708385764275 1107 82-83 -0.038879037033407116 1107 84-85 -0.10909984892841251 1107 86-87 -0.191217416527369 1107 88-89 -0.18160059553434138 1107 90-91 -0.16063385140429887 1107 92-93 -0.009073978905945523 1107 94-95 -0.08834201461080227 1107 96-97 -0.13964454715183194 1107 98-99 0.11621815424729576 1107 100-101 0.6518217586705681 1108 1 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-0.017332702093206365 2316 72-73 0.12093278545274444 2316 74-75 0.01647571584419083 2316 76-77 0.1465753836909478 2316 78-79 0.026093340841057966 2316 80-81 0.09574858642059425 2316 82-83 0.0841430246447068 2316 84-85 -0.006108518483674885 2316 86-87 -0.07149742312977381 2316 88-89 -0.0279390808033817 2316 90-91 0.1699674152509374 2316 92-93 0.3388210862609782 2316 94-95 0.3280836865403103 2316 96-97 0.23549438802871236 2316 98-99 0.40136120292093125 2316 100-101 9.696614395018344E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 26.0 5 66.0 6 166.0 7 334.0 8 824.0 9 1636.0 10 2627.0 11 4196.0 12 6268.0 13 8228.0 14 10808.0 15 14289.0 16 18244.0 17 23196.0 18 20765.0 19 15562.0 20 14042.0 21 14631.0 22 15615.0 23 17858.0 24 20712.0 25 24771.0 26 31064.0 27 38847.0 28 48406.0 29 60543.0 30 75238.0 31 94219.0 32 119076.0 33 153221.0 34 207258.0 35 290654.0 36 438958.0 37 635437.0 38 469630.0 39 39960.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.8896290311373 19.58626214842297 15.437677040315121 23.086431780124613 2 16.002768436555073 20.559352188569612 38.87423495215834 24.563644422716976 3 19.599591202826446 23.70220174453717 29.615292173549516 27.082914879086868 4 13.660899884864087 17.06636281498115 36.19100986655537 33.0817274335994 5 14.155165627226035 36.97676683342209 34.14940432492655 14.71866321442532 6 32.849378940136816 36.081899694900045 16.727219123864046 14.341502241099095 7 29.059562238416447 32.163874286017766 20.228719431278975 18.547844044286816 8 26.63728238393299 34.9582181236591 19.322236891289503 19.082262601118405 9 26.03760198652004 16.789723421885625 19.960556928274617 37.21211766331972 10-11 25.121059522747686 26.509839660815455 26.968374731650258 21.400726084786605 12-13 25.990305007388613 23.554249589630167 27.53785420197771 22.91759120100351 14-15 22.805881259536502 25.257217442103517 25.635443341351767 26.30145795700821 16-17 22.442260122734474 28.347554536361773 26.44219475876269 22.767990582141064 18-19 22.52165011346777 26.93262880957909 28.604210256832747 21.941510820120392 20-21 22.99683257086714 26.059100605365703 28.88466254828253 22.059404275484628 22-23 22.797530310246607 25.851858105865343 28.807628448957622 22.54298313493043 24-25 22.801259961002827 26.081141692844927 28.784559775379016 22.33303857077323 26-27 22.45232424872807 26.371410328666627 28.774990267649432 22.401275154955876 28-29 22.497610903607836 26.59209826156852 28.632269125793286 22.27802170903036 30-31 22.50583679841819 26.25815432569914 28.618083078969892 22.61792579691278 32-33 22.435349244467382 26.203463064930254 28.631258004533265 22.7299296860691 34-35 22.985189613392535 26.309883781496413 28.401139375733216 22.303787229377836 36-37 22.999453938485694 26.044274558639994 28.451898585074275 22.50437291780004 38-39 22.65898254169166 26.353306565879052 28.429191413625254 22.55851947880403 40-41 22.90930612739574 26.312334930438436 28.2949569653142 22.48340197685162 42-43 22.786284142976847 26.269590469844406 28.118889283477998 22.825236103700743 44-45 22.7977448067984 26.464459361652725 28.199923878207855 22.537871953341018 46-47 23.09698924013702 26.764725107050523 27.63226161385008 22.506024038962373 48-49 23.076324692806356 26.734647466907784 27.529858058050017 22.659169782235843 50-51 23.098282902078644 26.941769552508717 27.439471940812926 22.520475604599717 52-53 23.226287346828727 26.866158416394377 27.269814985916106 22.637739250860797 54-55 23.085993113633222 26.922058229766616 27.320455042183596 22.67149361441657 56-57 23.13299049022298 27.066233448786985 27.218494054942504 22.58228200604753 58-59 23.12487105935253 26.742290285483953 27.325033924582232 22.80780473058128 60-61 23.041072404897122 26.847451383843797 27.508427526674968 22.603048684584113 62-63 22.795770270150655 26.997311906660247 27.63838948620514 22.568528336983956 64-65 22.829320371294596 26.917411259897367 27.492495058551818 22.76077331025622 66-67 22.841337809857567 26.891248649314804 27.326889308156403 22.940524232671226 68-69 22.877356081811183 26.813339561067313 27.4964441318473 22.812860225274207 70-71 22.959792986854353 26.747754305000548 27.334906607820937 22.957546100324162 72-73 22.910464668761605 26.750416653403775 27.38136718829125 22.957751489543366 74-75 22.850121093566482 27.19546346800808 26.978621895977323 22.975793542448113 76-77 23.05387284937213 26.888916653446348 27.22494534278297 22.832265154398552 78-79 22.974023268212207 26.924407247502725 27.228758241137225 22.872811243147847 80-81 22.979861768817162 27.05499901613605 27.04010488193973 22.925034333107057 82-83 23.012703290695118 27.051767241548063 27.01339982777219 22.922129639984632 84-85 22.980790246486475 27.129785160970965 26.986918874827143 22.902505717715417 86-87 23.041429864117834 27.110251998537482 26.89766589341996 22.950652243924726 88-89 22.939911445435722 27.06837820411126 26.774359484142085 23.21735086631093 90-91 22.978891684930215 26.975382768448625 26.906777424858426 23.13894812176274 92-93 22.97654001624246 26.77084398985689 27.118638874623453 23.1339771192772 94-95 22.817388042069886 26.82062492042277 27.077995559335164 23.28399147817218 96-97 22.823345695748415 26.707463544266048 27.089757669883348 23.379433090102193 98-99 22.636480267284583 26.924312319748783 26.910762892339758 23.528444520626877 100-101 22.63407630159043 26.68390192315091 27.144609658212808 23.537412117045843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 257.0 1 194.0 2 166.0 3 211.0 4 195.5 5 205.0 6 392.0 7 832.0 8 1402.5 9 1811.5 10 1805.0 11 1716.5 12 1837.0 13 2056.5 14 2324.0 15 2537.5 16 2754.5 17 3028.5 18 3325.5 19 3687.5 20 4147.0 21 4833.0 22 5775.5 23 7064.5 24 8762.0 25 11191.0 26 14464.0 27 18166.5 28 21714.5 29 25539.0 30 30086.0 31 35704.0 32 42623.5 33 47178.5 34 50884.5 35 56120.5 36 62525.5 37 69818.5 38 75499.5 39 79761.0 40 83615.0 41 88920.5 42 92526.5 43 95682.5 44 101209.0 45 105337.5 46 108483.5 47 113351.5 48 116884.0 49 117008.5 50 114652.5 51 109900.5 52 108112.5 53 109671.5 54 110677.0 55 104162.0 56 92796.5 57 82808.0 58 74771.0 59 65505.5 60 53512.0 61 44198.0 62 36431.5 63 27773.0 64 19795.5 65 14198.0 66 10431.5 67 8146.5 68 6362.0 69 4927.0 70 3723.0 71 2597.5 72 1876.5 73 1539.5 74 1168.5 75 784.5 76 540.5 77 329.5 78 188.0 79 125.0 80 79.0 81 46.5 82 33.5 83 17.0 84 11.0 85 7.5 86 4.0 87 4.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05535511360734909 2 0.0 3 0.0 4 0.0 5 0.019132579241900486 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003693745280687193 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.510933826468187E-4 24-25 1.872405441823001E-4 26-27 0.001004290191523246 28-29 0.003234154854057911 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015217549681725118 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.5319680887642737E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.234154854057911E-4 84-85 0.001225574471011419 86-87 0.0 88-89 0.0 90-91 0.010519514209514679 92-93 0.0011745088680526097 94-95 0.0 96-97 0.0 98-99 1.5319680887642737E-4 100-101 0.013447275445819737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2937398.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.88598162176366 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34492438566433 36.81342492716798 2 14.937029175125588 15.201707841783769 3 5.23434807244997 7.990648194498099 4 2.472958086065874 5.033555988757597 5 1.350746787172773 3.4367038093865014 6 0.7993833545057347 2.4406444011673565 7 0.5271247243603111 1.8776281325323219 8 0.38869436539679797 1.5823275467251645 9 0.2961320844298865 1.3562074625322393 >10 1.4870859159393355 13.768463964364544 >50 0.10960397526079432 3.813692747090759 >100 0.04815258442173053 4.390027571388801 >500 0.0026782380400258146 0.9474176615274517 >1k 0.0010043392649086498 0.8272144928424803 >5k 1.3391190198781998E-4 0.5203352582351151 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8357 0.2845034959511786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6915 0.23541242964011005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.14945199799278136 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.80874706117455E-5 2 0.0 0.0 0.0 0.0 6.80874706117455E-5 3 0.0 0.0 0.0 0.0 6.80874706117455E-5 4 0.0 0.0 0.0 0.0 6.80874706117455E-5 5 0.0 0.0 0.0 0.0 6.80874706117455E-5 6 0.0 0.0 0.0 0.0 6.80874706117455E-5 7 0.0 0.0 0.0 0.0 6.80874706117455E-5 8 0.0 0.0 0.0 0.0 6.80874706117455E-5 9 0.0 0.0 0.0 3.404373530587275E-5 6.80874706117455E-5 10-11 0.0 0.0 0.0 3.404373530587275E-5 6.80874706117455E-5 12-13 0.0 0.0 0.0 3.404373530587275E-5 8.510933826468188E-5 14-15 0.0 1.7021867652936374E-5 0.0 3.404373530587275E-5 1.36174941223491E-4 16-17 0.0 3.404373530587275E-5 0.0 3.404373530587275E-5 1.7021867652936376E-4 18-19 1.7021867652936374E-5 3.404373530587275E-5 0.0 3.404373530587275E-5 1.7021867652936376E-4 20-21 3.404373530587275E-5 1.36174941223491E-4 0.0 3.404373530587275E-5 2.3830614714110922E-4 22-23 3.404373530587275E-5 1.36174941223491E-4 0.0 6.80874706117455E-5 2.8937175009991834E-4 24-25 3.404373530587275E-5 1.36174941223491E-4 0.0 1.5319680887642737E-4 3.0639361775285475E-4 26-27 3.404373530587275E-5 1.36174941223491E-4 0.0 2.893717500999184E-4 3.0639361775285475E-4 28-29 5.106560295880912E-5 1.36174941223491E-4 0.0 9.532245885644369E-4 3.404373530587275E-4 30-31 6.80874706117455E-5 1.7021867652936376E-4 0.0 0.00272349882446982 3.404373530587275E-4 32-33 6.80874706117455E-5 2.042624118352365E-4 0.0 0.004970385354657421 4.08524823670473E-4 34-35 6.80874706117455E-5 2.042624118352365E-4 0.0 0.007540687370250814 4.4256855897634574E-4 36-37 1.0213120591761825E-4 2.042624118352365E-4 0.0 0.010196098724108888 4.4256855897634574E-4 38-39 1.36174941223491E-4 2.2128427948817287E-4 0.0 0.013055772489802199 4.766122942822185E-4 40-41 1.36174941223491E-4 2.3830614714110925E-4 0.0 0.015506921431825037 5.106560295880913E-4 42-43 1.36174941223491E-4 2.72349882446982E-4 0.0 0.018707032550577075 5.106560295880913E-4 44-45 1.36174941223491E-4 3.0639361775285475E-4 0.0 0.02200927487524673 5.276778972410276E-4 46-47 1.36174941223491E-4 3.0639361775285475E-4 0.0 0.027524359994798117 5.617216325469003E-4 48-49 1.36174941223491E-4 3.0639361775285475E-4 0.0 0.034111822776484495 6.80874706117455E-4 50-51 1.36174941223491E-4 3.0639361775285475E-4 0.0 0.04012054205797104 6.978965737703914E-4 52-53 1.36174941223491E-4 3.2341548540579116E-4 0.0 0.052137980620944116 7.659840443821368E-4 54-55 1.36174941223491E-4 3.404373530587275E-4 0.0 0.07554304864373163 8.17049647340946E-4 56-57 1.36174941223491E-4 3.404373530587275E-4 0.0 0.11430184128946776 8.17049647340946E-4 58-59 1.36174941223491E-4 3.404373530587275E-4 0.0 0.15717992590721447 8.17049647340946E-4 60-61 1.36174941223491E-4 3.744810883646002E-4 0.0 0.1996324638336378 8.340715149938823E-4 62-63 1.36174941223491E-4 3.744810883646002E-4 0.0 0.2521279036752936 8.851371179526915E-4 64-65 1.36174941223491E-4 3.744810883646002E-4 0.0 0.31880255927184536 8.851371179526915E-4 66-67 1.36174941223491E-4 3.744810883646002E-4 0.0 0.4216827273661928 9.021589856056278E-4 68-69 1.36174941223491E-4 3.744810883646002E-4 0.0 0.5487169256600569 9.191808532585642E-4 70-71 1.36174941223491E-4 3.744810883646002E-4 0.0 0.6659295063181769 9.191808532585642E-4 72-73 1.36174941223491E-4 3.744810883646002E-4 0.0 0.7857804764625018 9.872683238703096E-4 74-75 1.36174941223491E-4 3.744810883646002E-4 0.0 0.9142445116392126 9.872683238703096E-4 76-77 1.36174941223491E-4 3.744810883646002E-4 0.0 1.0635092690878118 9.872683238703096E-4 78-79 1.36174941223491E-4 3.744810883646002E-4 0.0 1.2053695141073835 9.872683238703096E-4 80-81 1.7021867652936376E-4 3.744810883646002E-4 0.0 1.3614259967495042 0.0010213120591761825 82-83 1.7021867652936376E-4 3.744810883646002E-4 0.0 1.5416875751941004 0.0011064213974408643 84-85 1.7021867652936376E-4 3.744810883646002E-4 0.0 1.78069161890898 0.0011234432650938007 86-87 1.7021867652936376E-4 3.744810883646002E-4 0.0 2.04155174069023 0.0011574870003996734 88-89 1.7021867652936376E-4 3.744810883646002E-4 0.0 2.380286906983664 0.0011915307357055463 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6880 0.0 36.553017 1 GTATCAA 10650 0.0 34.63922 1 ATCAACG 12895 0.0 28.29148 3 TCAACGC 12910 0.0 28.221813 4 TATCAAC 13215 0.0 27.642351 2 CAACGCA 13185 0.0 27.63319 5 AACGCAG 13655 0.0 26.67934 6 TAAGGTG 1495 0.0 23.195164 5 ACGCAGA 15625 0.0 23.16559 7 CGCAGAG 15590 0.0 23.156658 8 GTACATG 9140 0.0 21.949383 1 GAGTACT 9285 0.0 21.870695 12-13 TACATGG 8940 0.0 21.732094 2 GTAAGGT 1365 0.0 21.576193 4 ACATGGG 8785 0.0 21.196302 3 AGAGTAC 13665 0.0 20.891674 10-11 GCAGAGT 17270 0.0 20.68397 9 GTACTTT 9800 0.0 20.646906 14-15 AGTACTT 9785 0.0 20.461863 12-13 GGTAAGG 1495 0.0 18.746777 3 >>END_MODULE