FastQCFastQC Report
Thu 26 May 2016
SRR937504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110618
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78680.2529400910044242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.19311275122821253No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46050.14804132169234538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.12090844970356372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.041.0598141
GTATCAA101950.038.6884731
ATCAACG123350.031.583513
TCAACGC124800.031.1784864
TATCAAC125500.031.0050812
CAACGCA127200.030.6265745
AACGCAG131550.029.6114466
CGCAGAG150850.025.3174158
ACGCAGA153950.025.024817
TACATGG95750.022.725812
GTACATG100400.022.7194731
GCAGAGT164900.022.2669359
ACATGGG99350.021.1368393
GAGTACT91300.020.96799512-13
AGAGTAC135400.020.27765810-11
TATACCG2151.0781459E-619.8872955
GTACTTT95950.019.72872414-15
AGTACTT93700.019.39163812-13
CAGAGTA157700.019.1874110-11
CATGGGG64700.018.28444
Produced by FastQC (version 0.11.5)