FastQCFastQC Report
Thu 26 May 2016
SRR937504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110618
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87490.28126243723915956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71730.23059726395205069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.14379779195002407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66200.043.72011
GTATCAA106000.036.6300931
ATCAACG134200.028.7391553
TATCAAC136400.028.5193752
TCAACGC135700.028.4214764
CAACGCA138850.027.7424895
AACGCAG143450.026.9186636
ACGCAGA163700.023.3619167
CGCAGAG163900.023.2464498
GTAAGGT14450.022.0230484
AGAGTAC149250.020.76956710-11
GAGTACT103750.020.7429912-13
GCAGAGT182300.020.5613489
TAAGGTG17900.020.4318715
TACATGG92350.020.0584872
GTACTTT105550.020.02466414-15
AGTACTT109100.019.48630512-13
GTACATG99000.019.202011
CAGAGTA173600.018.49942410-11
ACATGGG95400.018.471243
Produced by FastQC (version 0.11.5)