FastQCFastQC Report
Thu 26 May 2016
SRR937503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16935
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.3424859757897845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.24210215529967524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.21848243283141425No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN330.19486271036315322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA602.1713277E-855.4166681
TCAACGC802.091183E-741.56254
ATCAACG802.091183E-741.56253
CAACGCA802.091183E-741.56255
AACGCAG853.3655851E-739.1176456
TATCAAC853.3655851E-739.1176452
ACGCAGA958.0459176E-735.07
CGCAGAG958.0459176E-735.08
GCAGAGT1001.2017827E-633.259
AGTACTT659.651154E-625.57692312-13
AGAGTAC1101.9863364E-923.7510-11
GAGTACT704.719105E-420.35714112-13
CAGAGTA1101.2676701E-619.43181810-11
TACTTTT901.1890155E-418.47222314-15
GTACTTT800.00115886417.812514-15
ACTTTTT1000.005139104614.2516-17
CTTTTTT1500.00567670811.08333416-17
CTTATAC3300.0040453697.196969594-95
CTCTTAT3550.0082209596.69014192-93
GTCTCTT3600.0094094166.59722390-91
Produced by FastQC (version 0.11.5)