FastQCFastQC Report
Thu 26 May 2016
SRR937503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16935
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.377915559492176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.3247711839385887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.2716268083850015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1535281960436965No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT210.12400354295837024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT180.10628875110717448No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG180.10628875110717448RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT170.10038382049010923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACAT250.004936317457.02
TACATGG459.357962E-442.2222252
ACATGGG500.001571171238.03
GTACATG500.001571171238.01
GTATCAA651.2736296E-436.5384641
AACGCAG701.969295E-433.928576
GTACTTT601.433782E-731.66666814-15
ACGCAGA752.952909E-431.6666687
TCAACGC752.952909E-431.6666684
GAGTACT601.433782E-731.66666812-13
CGCAGAG752.952909E-431.6666688
ATCAACG752.952909E-431.6666683
CAACGCA752.952909E-431.6666685
TATCAAC804.3110506E-429.6874982
GCAGAGT856.1477773E-427.9411759
CAGAGTA801.7722778E-623.74999810-11
ACTTTTT953.096102E-722.516-17
AGTACTT652.8605884E-421.92307712-13
AGAGTAC652.8605884E-421.92307710-11
GCTTGAA804.8080867E-520.7812556-57
Produced by FastQC (version 0.11.5)