FastQCFastQC Report
Thu 26 May 2016
SRR937502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.4065591543569589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.3320233093915165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.27781542214392196No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT290.19650359127253014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.16262366174278356No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG210.14229570402493563RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT200.1355197181189863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAT156.405152E-495.306134
GTTAGTA200.00200767171.479593
ATGAGTA200.002035035871.237299
GTATCAA701.9201051E-434.0379031
AGTACTT657.734343E-932.8787512-13
AGAGTAC753.1162926E-828.49491510-11
TACTTTT753.1162926E-828.49491514-15
TCAACGC855.9951434E-428.0312123
ATCAACG855.9951434E-428.0312122
CAACGCA855.9951434E-428.0312124
AACGCAG950.001148144725.0805595
TATCAAC950.001148144725.0805591
GCAGAGT950.001171302924.995548
CGCAGAG950.001171302924.995547
ACGCAGA1000.001579428223.7457626
CAGAGTA1053.8459236E-422.6150139
GAGTACT652.8520238E-421.91916512-13
GTCTTCT704.7050836E-420.35351248-49
TGCCGTC650.00740185718.26597244-45
TGCTTGA650.00740185718.26597254-55
Produced by FastQC (version 0.11.5)