FastQCFastQC Report
Thu 26 May 2016
SRR937501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17803
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.376341066112453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.22468123349997193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.17974498679997752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310.17412795596247824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCC200.002041693571.239449
GTACATG250.004888206757.152541
GATCCTG250.001595969337.99436626-27
ACGCAGA801.0004778E-535.720341
AGAGTAC801.0004778E-535.720345
CGCAGAG851.5156793E-533.619142
CAGAGTA902.2411452E-531.7514114
GAGTACT600.00385075331.6619726
AGTACTT458.7848085E-431.661977
GCAGAGT953.242916E-530.0802843
TACTTTT500.001623796128.4957759
CTTTTTT1250.0014514713.29802816-17
ACGAAGG1450.004420904411.46381794-95
CGACGAA1750.001431988910.85553392-93
GACGAAG1550.007265259510.72421692-93
GCCGACG1900.00283893979.99851790-91
CTGCCGA2200.0095010498.63508388-89
Produced by FastQC (version 0.11.5)