FastQCFastQC Report
Thu 26 May 2016
SRR937501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17803
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.4100432511374487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.376341066112453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.2696174801999663No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT280.15727686344998035RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT270.15165983261248103No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT250.14042577093748243No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT210.11795764758748527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT180.10110655507498736No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG180.10110655507498736RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGTT250.00494065557.0000046
GTATCAA1001.2066339E-633.2500041
TCAACGC1153.5914054E-628.9130444
CAACGCA1153.5914054E-628.9130445
AACGCAG1205.002281E-627.7083346
ATCAACG1205.002281E-627.7083343
TATCAAC1256.8712307E-626.6000022
AGTACTT751.0126732E-625.33333412-13
ACGCAGA1351.2488164E-524.6296317
CGCAGAG1401.6553524E-523.758
GCAGAGT1452.1719421E-522.9310349
GTACTTT904.9407518E-621.11111314-15
GAGTACT904.9407518E-621.11111312-13
AGAGTAC1301.3724275E-820.09615510-11
ACTTTTT1051.870767E-518.09523816-17
CAGAGTA1351.606087E-417.5925949
TACTTTT1154.087024E-516.5217414-15
CTTTTTT1301.1650476E-414.61538516-17
Produced by FastQC (version 0.11.5)