FastQCFastQC Report
Thu 26 May 2016
SRR937500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28811
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.2846135156710978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.20478289542188746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.18742841275901564No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN370.12842317170525147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC200.00203735171.3913653
TGTAGGA250.004932725357.1130872
TAGGACG250.004932725357.1130874
AGTACAT701.9807616E-433.9958882
GTACATG856.182717E-427.9966133
GAGTACA1000.001596223723.7971211
TACATGG1000.001596223723.7971214
GGTATCA1201.6326991E-423.7971211
GAGTACT901.8804349E-723.75566312-13
GTATCAA1553.68154E-521.4941711
GTACTTT1101.296894E-619.43645314-15
GCAGAGT1759.497008E-519.004539
GGCTTAG757.677033E-418.9714882-83
AGTACTT1051.8998291E-518.0995512-13
ACGCAGA1851.4543661E-417.9772597
AACGCAG1851.4543661E-417.9772596
CGCAGAG1851.4543661E-417.9772598
TATCAAC1901.7599919E-417.534722
TCAACGC1952.14672E-417.0851144
AGGCTTA850.001777633716.7395482-83
Produced by FastQC (version 0.11.5)