FastQCFastQC Report
Thu 26 May 2016
SRR937500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28811
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.3748568255180313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.3193224809968415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.21866648155218493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT310.10759779250980528No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT300.10412689597723093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAGTG200.002053541871.255
GGTATCA504.0977102E-757.01
CAGTACA250.00497184257.04
GTATCAA1300.054.8076931
CAACGCA1450.049.137935
TCAACGC1450.049.137934
ACGCAGA1500.047.57
AACGCAG1500.047.56
CGCAGAG1500.047.58
ATCAACG1600.044.5312543
TATCAAC1650.043.181822
GCAGAGT1700.041.9117669
GAGTACA851.5778727E-533.5294151
AGTACAT851.5778727E-533.5294152
CAGAGTA1600.031.17187710-11
GAGTACT1054.0017767E-1127.14285712-13
GTACTTT1054.0017767E-1127.14285714-15
AGAGTAC1400.027.14285510-11
TACATGG1151.2398738E-424.7826084
GTACATG1201.655092E-423.753
Produced by FastQC (version 0.11.5)