FastQCFastQC Report
Thu 26 May 2016
SRR937499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297669
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32401.0884573133245317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.7887956085450618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.6634886400666512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11920.4004447893465561No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.22340250412370785No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.1978707893667127No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5120.17200313099449388No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.1353852769351192No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.13336961524377747No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.11052544940857126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT200.002069672671.275026
GGTATCA12400.044.090171
GTATCAA20450.035.1034051
GCTAAGG550.00256575434.5750581
GCGTAAA550.00256575434.5750581
CGTAAAA650.00583197729.2361052
TAGGGTC650.0058367829.2311844
ACGCAGC856.3764973E-427.9556968
TATCAAC25950.027.644742
TCAACGC26150.027.6103344
ATCAACG26050.027.533983
CAACGCA26450.027.1221545
AACGCAG26600.026.795126
CTACGGC458.94609E-426.38704174-75
GTACATG13050.024.7721671
GTATAGG1551.3943118E-624.5371381
CGCAGAG28900.024.1734548
ACGCAGA29150.024.1291737
TCTCGCC701.7762488E-523.75233842-43
ATAGGAC1000.001650632423.7503363