##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937499_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 297669 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80857932804558 33.0 31.0 34.0 30.0 34.0 2 31.916259335033207 33.0 31.0 34.0 30.0 34.0 3 31.927056562826497 33.0 31.0 34.0 30.0 34.0 4 35.584168993076204 37.0 35.0 37.0 33.0 37.0 5 35.600935938912016 37.0 35.0 37.0 33.0 37.0 6 35.664526705837694 37.0 35.0 37.0 35.0 37.0 7 35.61230427085118 37.0 35.0 37.0 33.0 37.0 8 35.60198072355536 37.0 35.0 37.0 33.0 37.0 9 37.2277496145047 39.0 37.0 39.0 34.0 39.0 10-11 37.13161263013616 39.0 37.0 39.0 34.0 39.0 12-13 37.022377204210045 39.0 37.0 39.0 33.0 39.0 14-15 38.139332614414 40.0 38.0 41.0 33.0 41.0 16-17 38.10503949017197 40.0 38.0 41.0 33.0 41.0 18-19 38.12878566461405 40.0 38.0 41.0 33.5 41.0 20-21 38.059962575881265 40.0 38.0 41.0 34.0 41.0 22-23 38.07192216858323 40.0 38.0 41.0 34.0 41.0 24-25 38.048847881371586 40.0 38.0 41.0 34.0 41.0 26-27 37.95743762366924 40.0 38.0 41.0 33.5 41.0 28-29 37.82303666152673 40.0 37.5 41.0 33.0 41.0 30-31 37.54415642878499 40.0 37.0 41.0 33.0 41.0 32-33 37.23590800520041 39.0 36.0 41.0 33.0 41.0 34-35 37.177431979816504 39.0 36.0 41.0 33.0 41.0 36-37 37.030246683396655 39.0 36.0 41.0 32.0 41.0 38-39 36.86204307469035 39.0 35.0 41.0 31.5 41.0 40-41 36.62979685489587 39.0 35.0 41.0 31.0 41.0 42-43 36.341810870463505 39.0 35.0 41.0 30.5 41.0 44-45 36.03404788540291 39.0 35.0 41.0 30.0 41.0 46-47 35.54864463548438 39.0 35.0 40.5 28.0 41.0 48-49 35.18614971663156 39.0 35.0 40.0 26.5 41.0 50-51 34.20012497102486 38.0 34.0 39.5 23.5 40.5 52-53 34.29519197497892 38.0 34.5 39.5 24.0 40.5 54-55 34.59346959206367 38.5 35.0 40.0 23.5 41.0 56-57 34.35951509898579 38.0 34.5 40.0 22.5 41.0 58-59 34.14037403962119 38.0 34.0 40.0 20.5 41.0 60-61 33.84171344681509 38.0 34.0 40.0 18.5 41.0 62-63 33.47620343401563 38.0 33.5 40.0 13.0 41.0 64-65 33.097195206756496 37.5 33.0 40.0 8.0 41.0 66-67 32.68204448565353 37.0 33.0 39.5 4.0 41.0 68-69 32.209559947458416 36.5 32.0 39.0 2.0 41.0 70-71 31.772334371399104 36.0 31.5 39.0 2.0 40.0 72-73 31.275438490403772 35.5 31.0 38.5 2.0 40.0 74-75 30.73152730045789 35.0 30.5 38.0 2.0 40.0 76-77 30.23695782899798 35.0 30.0 37.0 2.0 39.0 78-79 29.70546983394307 34.5 29.0 37.0 2.0 39.0 80-81 29.14017919232436 34.0 29.0 36.0 2.0 38.5 82-83 28.580391307123016 34.0 28.5 36.0 2.0 37.5 84-85 28.024869905835004 34.0 27.0 35.0 2.0 37.0 86-87 27.456107286952957 34.0 26.0 35.0 2.0 36.5 88-89 26.917750924684803 33.0 25.5 35.0 2.0 36.0 90-91 26.36892823908435 33.0 24.5 35.0 2.0 36.0 92-93 25.72589016659444 32.5 21.5 35.0 2.0 35.0 94-95 24.969748277449114 32.0 18.5 34.5 2.0 35.0 96-97 24.169206400397755 31.5 4.5 34.0 2.0 35.0 98-99 23.04788204347782 31.0 2.0 34.0 2.0 35.0 100-101 19.99431751374849 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.00657594916859594 1101 2 0.18174127844139676 1101 3 0.20262132098275387 1101 4 0.2069097709306078 1101 5 0.2493128901183752 1101 6 0.36022400169817104 1101 7 0.2826352744263474 1101 8 0.17274890658673314 1101 9 0.3057782049894513 1101 10-11 0.28719970974807296 1101 12-13 0.4799636652669719 1101 14-15 0.5256742648713129 1101 16-17 0.41561386260359257 1101 18-19 0.5944181430657451 1101 20-21 0.6579198604970884 1101 22-23 0.4818800588666434 1101 24-25 0.2194193790586212 1101 26-27 0.5058615645049755 1101 28-29 0.6496449275613543 1101 30-31 0.5803884438884026 1101 32-33 0.5986041775737831 1101 34-35 0.8959356774655802 1101 36-37 0.8278276391634733 1101 38-39 0.6833490712332377 1101 40-41 0.9964467731623117 1101 42-43 0.9513137881447449 1101 44-45 1.262739576961124 1101 46-47 1.2271428793263581 1101 48-49 1.1424347750950261 1101 50-51 1.263734966006183 1101 52-53 1.3884620613424943 1101 54-55 1.7031908146534889 1101 56-57 1.868405422203658 1101 58-59 1.6608436906880826 1101 60-61 1.7939993710356745 1101 62-63 2.1835958560034854 1101 64-65 2.0008873163776 1101 66-67 1.7275580812874693 1101 68-69 1.9344648916477674 1101 70-71 2.070693996827103 1101 72-73 2.0934955851681174 1101 74-75 2.170945103148039 1101 76-77 2.171846109213373 1101 78-79 2.0900123369537305 1101 80-81 2.103428142076254 1101 82-83 2.3431948113609415 1101 84-85 2.465969772245483 1101 86-87 2.3853198881381132 1101 88-89 2.6054148269155597 1101 90-91 2.3170042411278793 1101 92-93 2.590807254024476 1101 94-95 2.5846828149853813 1101 96-97 2.443645007301818 1101 98-99 2.9489087996259435 1101 100-101 2.81548409018254 1102 1 0.2439842317205141 1102 2 0.20944512953786543 1102 3 0.39538341524963627 1102 4 0.14091880794921963 1102 5 0.1028966069031867 1102 6 0.13394203013576345 1102 7 -0.07497779194184773 1102 8 -0.07251909490561559 1102 9 0.08754249632966804 1102 10-11 0.0020817725097472817 1102 12-13 0.16798132476206717 1102 14-15 0.34485927502760205 1102 16-17 0.500907980250652 1102 18-19 0.4798822229894668 1102 20-21 0.35368945797667095 1102 22-23 0.3172322124130531 1102 24-25 0.3626644149165159 1102 26-27 0.3535691464806874 1102 28-29 0.453995547301858 1102 30-31 0.4460560640019864 1102 32-33 0.22781074392124623 1102 34-35 0.60571265379523 1102 36-37 0.803740668222872 1102 38-39 0.7052389688413285 1102 40-41 0.7554630646683407 1102 42-43 0.9657895996109787 1102 44-45 1.225778999918468 1102 46-47 1.2626276863569856 1102 48-49 1.5253929477932857 1102 50-51 1.3644645601694378 1102 52-53 1.46347346125129 1102 54-55 1.7767409535251062 1102 56-57 1.8023647141656909 1102 58-59 1.953143570470857 1102 60-61 1.9187910636112235 1102 62-63 2.005268118989015 1102 64-65 2.2314079813031924 1102 66-67 2.184265373083683 1102 68-69 2.1790074443982554 1102 70-71 2.024447675888581 1102 72-73 2.28863093317624 1102 74-75 2.2123827352833523 1102 76-77 2.285575744830833 1102 78-79 2.190464187884377 1102 80-81 2.523599762521471 1102 82-83 2.175723932946152 1102 84-85 2.381231141063619 1102 86-87 2.485328178980879 1102 88-89 2.3711239012429672 1102 90-91 2.390583397949168 1102 92-93 2.101647530938621 1102 94-95 2.0992767709610085 1102 96-97 2.093628425616288 1102 98-99 2.628938232117754 1102 100-101 2.0724048711799306 1103 1 0.32560212423192425 1103 2 0.4717684854964723 1103 3 0.3973863083173974 1103 4 0.30042698642925103 1103 5 0.27792720665839 1103 6 0.29311639310449067 1103 7 0.26395622385875583 1103 8 0.20649503661769586 1103 9 0.18184537827025338 1103 10-11 0.3779024023083437 1103 12-13 0.23680295600719603 1103 14-15 0.4141816295396694 1103 16-17 0.41358740608797007 1103 18-19 0.32885852208816857 1103 20-21 0.39761401567112387 1103 22-23 0.09568998475087653 1103 24-25 0.4695131964899062 1103 26-27 0.34970245014152823 1103 28-29 0.16989072923131232 1103 30-31 0.5258101532419985 1103 32-33 0.6242444815422559 1103 34-35 0.4770267741913514 1103 36-37 0.4581025242371197 1103 38-39 0.7604576180012259 1103 40-41 0.680938751771933 1103 42-43 1.0203962536668314 1103 44-45 0.9358045924410021 1103 46-47 0.7002876396228217 1103 48-49 0.9317020609567379 1103 50-51 1.0385215313500282 1103 52-53 1.05674707869872 1103 54-55 1.2039413541037192 1103 56-57 0.9600369073335955 1103 58-59 1.1375488225015715 1103 60-61 0.9866112483224683 1103 62-63 1.4331361505902223 1103 64-65 1.2941652974264741 1103 66-67 1.3289465792704007 1103 68-69 1.56520417300624 1103 70-71 1.5771627087137716 1103 72-73 1.8360605537392765 1103 74-75 2.1580264428609546 1103 76-77 1.9310636718365082 1103 78-79 2.287882681263703 1103 80-81 2.158043959695167 1103 82-83 2.4438890603524044 1103 84-85 1.9723071397283611 1103 86-87 1.9963997267721325 1103 88-89 2.098340992597148 1103 90-91 1.675912206227796 1103 92-93 2.0430072727879605 1103 94-95 2.141198435587693 1103 96-97 1.978183894627083 1103 98-99 2.090132178929821 1103 100-101 1.7073219736612906 1104 1 0.415783110293912 1104 2 0.40476983769118746 1104 3 0.20086300896229048 1104 4 0.4124511179041832 1104 5 0.22256523453440025 1104 6 0.281973788569438 1104 7 0.28054661287477245 1104 8 0.26398931579389995 1104 9 0.4123425698658423 1104 10-11 0.5028113892024564 1104 12-13 0.29263949646902176 1104 14-15 0.4018038471390071 1104 16-17 0.4343318932941358 1104 18-19 0.654243377507342 1104 20-21 0.2930594598150833 1104 22-23 0.47429800430568747 1104 24-25 0.37796051290346355 1104 26-27 0.5781388449823837 1104 28-29 0.6269520460609357 1104 30-31 0.8107006774774916 1104 32-33 0.4644449704126501 1104 34-35 0.7815761292984931 1104 36-37 0.7101113135027788 1104 38-39 0.6631711087438958 1104 40-41 0.5522332181750969 1104 42-43 0.6007543252291399 1104 44-45 0.58783548488951 1104 46-47 0.6494308162402476 1104 48-49 0.9450029762885492 1104 50-51 0.8685090495526424 1104 52-53 0.8398953520500783 1104 54-55 0.9239655375861417 1104 56-57 0.9031667180263412 1104 58-59 0.9107051570137372 1104 60-61 0.8359910590152069 1104 62-63 0.7916627784246302 1104 64-65 0.7349612720468244 1104 66-67 0.9231516447998303 1104 68-69 0.9165407654755633 1104 70-71 1.227444849342021 1104 72-73 1.3392330105730643 1104 74-75 1.3505646683654327 1104 76-77 1.8555097920778216 1104 78-79 1.8703405551975507 1104 80-81 1.7601736663729284 1104 82-83 1.770203549638861 1104 84-85 1.7428826025950137 1104 86-87 1.9567180126052932 1104 88-89 2.2679894219709773 1104 90-91 2.459411530130435 1104 92-93 2.4343441252947216 1104 94-95 2.1947531474662014 1104 96-97 2.292153106193549 1104 98-99 2.834437358875732 1104 100-101 2.685992402203002 1105 1 0.24810059641066928 1105 2 0.16226171091345165 1105 3 -0.2549561025100111 1105 4 0.004215983135118506 1105 5 0.2397838448554097 1105 6 0.13786739550429417 1105 7 0.21312821926783698 1105 8 0.23461440044783188 1105 9 0.027878259964715824 1105 10-11 0.23944453291098 1105 12-13 0.30220115839507145 1105 14-15 0.3892073416534316 1105 16-17 0.12396093975220879 1105 18-19 0.45000729461420974 1105 20-21 0.5580389735628728 1105 22-23 0.2387611290517242 1105 24-25 0.308387676658036 1105 26-27 0.4788778738324453 1105 28-29 0.4261063532531324 1105 30-31 0.2315704120027462 1105 32-33 0.13949660931282892 1105 34-35 0.35993604396732337 1105 36-37 0.06001565455655111 1105 38-39 0.021346139219311056 1105 40-41 0.3108594541225429 1105 42-43 0.2929483859090851 1105 44-45 0.3163587816523403 1105 46-47 0.15833217747552197 1105 48-49 -0.13584999675763498 1105 50-51 0.0713398771295175 1105 52-53 0.0855483725024726 1105 54-55 0.4094854820531566 1105 56-57 0.6601591330337939 1105 58-59 0.4017910976882604 1105 60-61 0.6758300472313863 1105 62-63 0.9633832849937747 1105 64-65 0.993676394625723 1105 66-67 0.8600209729862058 1105 68-69 0.7663138929305227 1105 70-71 1.1735160602319041 1105 72-73 1.0633966296980795 1105 74-75 0.719854760468916 1105 76-77 0.6454579838697789 1105 78-79 0.7244818256837995 1105 80-81 1.0423506269580542 1105 82-83 1.3071676902321165 1105 84-85 1.3141739137151554 1105 86-87 1.3312608813578315 1105 88-89 1.6292924153341097 1105 90-91 1.5954631013074625 1105 92-93 1.7373163249425616 1105 94-95 1.9727989960372447 1105 96-97 2.1618876314542845 1105 98-99 2.7482765841229195 1105 100-101 3.1356783361052116 1106 1 0.2995623745079605 1106 2 -0.4135830051181806 1106 3 -0.21004836227516677 1106 4 -0.032532159687789886 1106 5 0.09923747533707683 1106 6 -0.05795096059443949 1106 7 0.17954326839316792 1106 8 0.2356448341885482 1106 9 0.26000143274209364 1106 10-11 -0.30065851046309433 1106 12-13 -0.05838475102261498 1106 14-15 0.13566672339318586 1106 16-17 0.08167721101987979 1106 18-19 -0.018666321489305915 1106 20-21 0.24905862369467968 1106 22-23 0.16913496082742796 1106 24-25 -0.17281289846777526 1106 26-27 0.08610289180512609 1106 28-29 0.4205348219806666 1106 30-31 0.4509389717918637 1106 32-33 0.42959965268690326 1106 34-35 0.35208198214129993 1106 36-37 0.3033178352419057 1106 38-39 0.35533247998134954 1106 40-41 0.47359609442927564 1106 42-43 0.20934546235288565 1106 44-45 -0.08545046828774616 1106 46-47 -0.1857907556661047 1106 48-49 -0.439654214346902 1106 50-51 -0.3055712180058805 1106 52-53 0.13431424837114747 1106 54-55 0.4364864405961697 1106 56-57 0.1605245775581139 1106 58-59 0.13557973895353825 1106 60-61 0.373637380085448 1106 62-63 -0.16523521488640824 1106 64-65 0.18644538575919967 1106 66-67 0.40252037389682016 1106 68-69 0.29321300627823277 1106 70-71 0.10300923061292622 1106 72-73 0.12886511759647234 1106 74-75 -0.017516435313496004 1106 76-77 0.6340633037835097 1106 78-79 0.44868503680290317 1106 80-81 0.5159847031622284 1106 82-83 0.21336826535792497 1106 84-85 0.16125634841440828 1106 86-87 0.1476879122708432 1106 88-89 0.337805475482682 1106 90-91 0.6935879515351182 1106 92-93 1.1139218844920435 1106 94-95 0.7747280638541589 1106 96-97 0.6919858821132792 1106 98-99 0.3670460545279042 1106 100-101 0.043538388825073326 1107 1 -0.2099817850361987 1107 2 0.21747112593595475 1107 3 -0.06360961583642322 1107 4 -0.12555210270773642 1107 5 0.09353946963906878 1107 6 -0.04783700048047734 1107 7 0.1440731829230799 1107 8 -0.1011073025635909 1107 9 -0.16720654446588412 1107 10-11 0.18709077728619405 1107 12-13 0.10436453672667056 1107 14-15 0.19528210800856982 1107 16-17 0.2749820543247239 1107 18-19 0.141803763980775 1107 20-21 -0.021382971746916724 1107 22-23 0.126542368234837 1107 24-25 0.4029705773157062 1107 26-27 0.20433651003875042 1107 28-29 0.4618453632912036 1107 30-31 0.039756635609528246 1107 32-33 -0.015557042469787064 1107 34-35 0.08591389097320956 1107 36-37 0.15516968709376044 1107 38-39 0.20611595576482955 1107 40-41 0.3989522197854001 1107 42-43 0.10414603215345153 1107 44-45 -0.18758722042449705 1107 46-47 0.10089015601480611 1107 48-49 0.04788139818870718 1107 50-51 -0.04203845447311494 1107 52-53 -0.0753723613154591 1107 54-55 -0.02469589558616292 1107 56-57 0.028971871005403216 1107 58-59 -0.14732624395244187 1107 60-61 0.23147213792020693 1107 62-63 0.05919498454378669 1107 64-65 -0.307500483186665 1107 66-67 -0.24106936969292292 1107 68-69 0.006033519098746609 1107 70-71 -0.46743236482866735 1107 72-73 0.2641927529241066 1107 74-75 0.2746488068517472 1107 76-77 0.10899207871228356 1107 78-79 0.4724742105920754 1107 80-81 0.23785080002832615 1107 82-83 -0.5874152104255508 1107 84-85 -0.11431345215538968 1107 86-87 0.013642328225259348 1107 88-89 0.15269151536872272 1107 90-91 -0.02842059547343112 1107 92-93 0.018693964264123508 1107 94-95 0.6818505709766676 1107 96-97 0.6610742012016004 1107 98-99 1.5587127211945706 1107 100-101 2.1317150270017144 1108 1 -0.03951521456962581 1108 2 -0.030965622500797707 1108 3 -0.30208965431646106 1108 4 -0.15013122728686312 1108 5 -0.26889439279479177 1108 6 0.06880561616213754 1108 7 -0.08031354146364578 1108 8 -0.10211740357369337 1108 9 0.04183626457692924 1108 10-11 -0.18748834729293407 1108 12-13 -0.02908904672691648 1108 14-15 -0.3014711387446738 1108 16-17 -0.3280963487536752 1108 18-19 -0.466709944532937 1108 20-21 -0.4874964878604402 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-1.4675712144330824 2316 74-75 -0.7505238234056328 2316 76-77 -0.7454021460209255 2316 78-79 -0.31606941778206377 2316 80-81 -0.48882842156615425 2316 82-83 -0.25241834915750516 2316 84-85 0.08330111156086772 2316 86-87 0.5476661825880953 2316 88-89 0.3095025637051947 2316 90-91 0.13749277552468797 2316 92-93 0.6311861174342432 2316 94-95 0.7464142872353001 2316 96-97 1.4096303845035436 2316 98-99 0.8663398398386377 2316 100-101 1.2743076195943068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1444.0 3 197.0 4 178.0 5 182.0 6 221.0 7 241.0 8 253.0 9 354.0 10 428.0 11 661.0 12 1049.0 13 1529.0 14 1778.0 15 2457.0 16 3297.0 17 4391.0 18 3583.0 19 2431.0 20 2419.0 21 2411.0 22 2546.0 23 2899.0 24 3209.0 25 3470.0 26 3811.0 27 4306.0 28 4922.0 29 6000.0 30 7058.0 31 8843.0 32 11455.0 33 14725.0 34 19873.0 35 28326.0 36 38839.0 37 55101.0 38 47027.0 39 5755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.78259404071727 22.81212080355939 16.064783605231227 21.340501550492114 2 13.855182218149723 23.15272097614219 41.45302888405279 21.539067921655292 3 16.503447973831108 28.382871638351848 32.67035214138209 22.44332824643496 4 10.541386361416073 19.407510215631515 41.38265582838413 28.66844759456828 5 10.475109235034715 39.91079279200369 36.59449462162736 13.019603351334233 6 25.586376203481382 41.457681570360094 19.28062205549064 13.675320170667888 7 23.341662345009663 36.09717329956828 24.09329391084141 16.467870444580644 8 23.022704464784844 36.243290025795936 22.526317916388837 18.207687593030382 9 24.575797379677343 17.03546529251288 23.60917449732242 34.77956283048735 10-11 21.552926198367146 29.001798561151077 30.346509309406404 19.098765931075366 12-13 22.78050012629452 27.97423591816115 30.11029721310095 19.13496674244338 14-15 20.075028581916023 29.453096191828017 29.105567033222375 21.36630819303359 16-17 17.552396217774305 32.64493144042456 30.16756014115999 19.635112200641146 18-19 17.289131982848748 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18.74021421813076 44-45 17.700020875842608 30.921836804783936 32.61682054182913 18.761321777544325 46-47 17.904798465784054 30.926271090838988 32.28689434081078 18.882036102566175 48-49 17.797317958631094 31.005967575722877 32.19190538092127 19.00480908472476 50-51 17.958908723475915 30.949646345570898 31.906702593465813 19.18474233748737 52-53 17.83301528163997 31.211229554722014 31.972700890488596 18.98305427314942 54-55 18.314173277405168 30.694931608114413 31.899093552825008 19.091801561655416 56-57 18.277768893452624 31.13213422999554 31.218658205048357 19.37143867150348 58-59 18.173387015330338 30.39339118959934 31.901682499713864 19.531539295356463 60-61 18.165705273328026 30.598902883843376 31.915172958224414 19.320218884604184 62-63 17.92099008167635 30.47207988526257 32.24835536283019 19.358574670230887 64-65 18.104912348619376 29.888994554926235 32.382114573777805 19.623978522676584 66-67 17.713884615255132 30.250032823751603 31.929430617322186 20.106651943671075 68-69 17.85641545290352 29.854112767615625 32.302399135483064 19.987072643997784 70-71 17.94643473342771 29.517791404846832 32.46302259463808 20.072751267087373 72-73 17.783983691800618 29.057312682116365 32.481899885027865 20.676803741055153 74-75 17.7145531338957 29.25457505172237 32.2373858867377 20.793485927644237 76-77 17.586012408347436 29.140254733102676 32.533609448527244 20.740123410022644 78-79 17.508615914566494 28.726730296198895 32.081844033005645 21.682809756228966 80-81 17.747463830331682 28.82647650023737 32.030484540560195 21.395575128870753 82-83 17.928384097175698 28.68507788801767 31.91068885529696 21.47584915950968 84-85 17.74683540042851 27.981607369170465 31.906535543994707 22.365021686406315 86-87 17.88581758687902 28.35960828275279 31.88526549313081 21.869308637237378 88-89 18.162275540831494 27.486579163994286 31.976688399246516 22.374456895927707 90-91 18.18420552047579 26.964041323607884 32.59141173282069 22.260341423095635 92-93 18.639399897648612 27.062178468500015 32.10887628949282 22.189545344358553 94-95 18.223075110258225 27.25936774063226 31.901996431336904 22.615560717772613 96-97 18.371885521885524 27.031313131313134 31.594949494949493 23.00185185185185 98-99 18.836356167473934 27.890716675168655 31.045373657991277 22.22755349936614 100-101 19.499747517252988 27.518094596869215 30.861471132805924 22.120686753071872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 865.0 1 559.0 2 307.0 3 358.5 4 295.0 5 294.5 6 574.0 7 1076.5 8 1502.5 9 1537.5 10 1218.5 11 960.5 12 943.0 13 919.0 14 824.0 15 764.0 16 734.5 17 757.5 18 810.0 19 862.5 20 992.5 21 1126.5 22 1272.0 23 1455.5 24 1744.5 25 2238.0 26 2912.0 27 3711.5 28 4315.0 29 5114.0 30 6160.0 31 7271.0 32 8539.5 33 9871.5 34 11430.5 35 12707.0 36 14066.0 37 15367.5 38 15181.0 39 14183.0 40 13362.0 41 12479.5 42 11548.5 43 11077.5 44 10744.0 45 10224.5 46 9298.5 47 8500.5 48 8299.5 49 7551.0 50 6368.0 51 5289.0 52 4449.5 53 3910.5 54 3359.5 55 2840.0 56 2435.5 57 2030.5 58 1693.0 59 1378.0 60 1102.0 61 913.5 62 721.5 63 534.0 64 415.0 65 333.5 66 267.5 67 223.0 68 189.0 69 155.0 70 124.0 71 97.5 72 74.0 73 65.5 74 59.0 75 42.5 76 32.0 77 22.5 78 15.0 79 15.5 80 12.5 81 8.5 82 6.0 83 4.0 84 2.0 85 0.5 86 0.0 87 1.0 88 1.0 89 0.5 90 1.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33224823545616106 2 0.2778253697899345 3 0.27816131340515804 4 0.2754737644833691 5 0.27950508786605255 6 0.2412075157305598 7 0.2401996848848889 8 0.2428872338066779 9 0.25498120395472823 10-11 0.25699686564607 12-13 0.24994204972637393 14-15 0.24036765669250074 16-17 0.2354964742717582 18-19 0.22323453231609608 20-21 0.22928151739012126 22-23 0.22743382750639135 24-25 0.22911354558250943 26-27 0.22457830677699053 28-29 0.24238331838384247 30-31 0.25834064010696445 32-33 0.24809435984264402 34-35 0.24607869815130226 36-37 0.23264095354235745 38-39 0.21869929351057718 40-41 0.21601174458878822 42-43 0.22911354558250943 44-45 0.2267619402759441 46-47 0.238351995001159 48-49 0.24557478272846686 50-51 0.25834064010696445 52-53 0.27194635652352106 54-55 0.24876624707309125 56-57 0.21550782916595282 58-59 0.20526154890163234 60-61 0.2074451824005859 62-63 0.21601174458878822 64-65 0.20542952070924414 66-67 0.21097259036043392 68-69 0.20979678770715124 70-71 0.21147650578326932 72-73 0.21517188555072916 74-75 0.21853132170296538 76-77 0.23230500992713382 78-79 0.2316331226966866 80-81 0.2237384477389315 82-83 0.21500391374311734 84-85 0.199382535635219 86-87 0.2076131542081977 88-89 0.21819537808774173 90-91 0.2175234908572945 92-93 0.21937118074102444 94-95 0.21466797012789376 96-97 0.22474627858460236 98-99 0.22827368654445038 100-101 0.2079490978234213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 297669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.36303457558272 #Duplication Level Percentage of deduplicated Percentage of total 1 88.69455595287276 57.08650771460705 2 6.4667266639966945 8.32436303731324 3 1.6890439146335472 3.2613597563170975 4 0.7323174759837564 1.8853670010818395 5 0.4489433450299869 1.4447678019321404 6 0.28554848066352995 1.1027260040371136 7 0.22864191113243343 1.0301261062150888 8 0.15257659358945103 0.7856234054897975 9 0.1312813495294724 0.7604699435005132 >10 1.0326438593744613 13.158309343294103 >50 0.10120366636216843 4.384374997868656 >100 0.03286510814853922 3.2434570745451996 >500 0.0015650051499304391 0.5929549889903544 >1k 0.0020866735332405856 2.939592824807787 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3240 1.0884573133245317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2348 0.7887956085450618 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1975 0.6634886400666512 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1192 0.4004447893465561 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 665 0.22340250412370785 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 589 0.1978707893667127 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 512 0.17200313099449388 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 403 0.1353852769351192 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 397 0.13336961524377747 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.11052544940857126 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.3594361522362086E-4 0.0 16-17 0.0 0.0 0.0 3.3594361522362086E-4 0.0 18-19 0.0 0.0 0.0 3.3594361522362086E-4 0.0 20-21 0.0 0.0 0.0 3.3594361522362086E-4 0.0 22-23 0.0 0.0 0.0 6.718872304472417E-4 0.0 24-25 0.0 0.0 0.0 0.0033594361522362084 0.0 26-27 0.0 0.0 0.0 0.005375097843577934 0.0 28-29 0.0 0.0 0.0 0.012933829186109403 0.0 30-31 0.0 0.0 0.0 0.028891150909231392 0.0 32-33 0.0 0.0 0.0 0.045520359862800626 0.0 34-35 0.0 0.0 0.0 0.05895810447174546 0.0 36-37 0.0 0.0 0.0 0.07844283415471548 0.0 38-39 0.0 0.0 0.0 0.10934964675528859 0.0 40-41 0.0 0.0 0.0 0.1451276417766042 0.0 42-43 0.0 0.0 0.0 0.21466797012789374 0.0 44-45 0.0 0.0 0.0 0.3048688308154359 0.0 46-47 0.0 0.0 0.0 0.3985970994628262 0.0 48-49 0.0 0.0 0.0 0.5277674195163083 0.0 50-51 0.0 0.0 0.0 0.7002744659336377 0.0 52-53 0.0 0.0 0.0 0.9858265388737154 0.0 54-55 0.0 0.0 0.0 1.2523977975536587 0.0 56-57 0.0 0.0 0.0 1.6288226184117258 0.0 58-59 0.0 0.0 0.0 2.152222770930127 0.0 60-61 0.0 0.0 0.0 2.681501936714942 0.0 62-63 0.0 0.0 0.0 3.3515414772784533 0.0 64-65 0.0 0.0 0.0 3.868726672915218 0.0 66-67 0.0 0.0 0.0 4.601587669525546 0.0 68-69 0.0 0.0 0.0 5.496037545058437 0.0 70-71 0.0 0.0 0.0 6.352525792071059 0.0 72-73 0.0 0.0 0.0 7.4601318914633366 0.0 74-75 0.0 0.0 0.0 8.542710191521454 0.0 76-77 0.0 0.0 0.0 9.423890294253013 0.0 78-79 0.0 0.0 0.0 10.439279871266407 0.0 80-81 0.0 0.0 0.0 11.884509303958424 0.0 82-83 0.0 0.0 0.0 13.136403186089247 0.0 84-85 0.0 0.0 0.0 14.752963862545311 0.0 86-87 0.0 0.0 0.0 16.41454098344134 0.0 88-89 0.0 1.6797180761181043E-4 0.0 18.452542925195438 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 20 0.0020696726 71.27502 6 GGTATCA 1240 0.0 44.09017 1 GTATCAA 2045 0.0 35.103405 1 GCTAAGG 55 0.002565754 34.575058 1 GCGTAAA 55 0.002565754 34.575058 1 CGTAAAA 65 0.005831977 29.236105 2 TAGGGTC 65 0.00583678 29.231184 4 ACGCAGC 85 6.3764973E-4 27.955696 8 TATCAAC 2595 0.0 27.64474 2 TCAACGC 2615 0.0 27.610334 4 ATCAACG 2605 0.0 27.53398 3 CAACGCA 2645 0.0 27.122154 5 AACGCAG 2660 0.0 26.79512 6 CTACGGC 45 8.94609E-4 26.387041 74-75 GTACATG 1305 0.0 24.772167 1 GTATAGG 155 1.3943118E-6 24.537138 1 CGCAGAG 2890 0.0 24.173454 8 ACGCAGA 2915 0.0 24.129173 7 TCTCGCC 70 1.7762488E-5 23.752338 42-43 ATAGGAC 100 0.0016506324 23.750336 3 >>END_MODULE