FastQCFastQC Report
Thu 26 May 2016
SRR937499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297669
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33241.1166765770033158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.8731174559661906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.7300054758809281No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13160.44210179763428503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.2546452603395046No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.24859827526547942No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.14915896515928767No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.1380728258569082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.12530696847841058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG301.2996711E-463.333115
GGTATCA15750.036.5041961
GGACGTC702.0497334E-433.928456
GTATCAA23350.031.948521
GTATAGA600.003939870731.6771981
GACGTGG753.0730732E-431.6665537
TAGGACG804.4858252E-429.6873934
TCGTACC856.3960766E-427.9410789
GTCTAAG700.00836817127.1518861
CTGGTCG1056.8891066E-527.1427619
CATAAGC700.00838190227.1427613
ATCAACG29300.025.7763583
TCAACGC29550.025.5582854
TATCAAC30000.025.1749112
CAACGCA30200.025.1654725
AGGACGT1701.1170414E-725.146975
GTCTAGA1151.2757526E-424.790851
CTATCCC1151.2786678E-424.782524
GGCCTAA1151.2786678E-424.782526
AACGCAG30700.024.6008916
Produced by FastQC (version 0.11.5)