##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 297669 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02000208285042 31.0 31.0 34.0 30.0 34.0 2 32.235620773409394 33.0 31.0 34.0 30.0 34.0 3 32.28082534627388 34.0 31.0 34.0 30.0 34.0 4 35.984234166137554 37.0 35.0 37.0 35.0 37.0 5 35.908905529295964 37.0 35.0 37.0 35.0 37.0 6 35.95097910766657 37.0 35.0 37.0 35.0 37.0 7 35.87638282790616 37.0 35.0 37.0 35.0 37.0 8 35.85946806687966 37.0 35.0 37.0 35.0 37.0 9 37.51244503122596 39.0 37.0 39.0 35.0 39.0 10-11 37.44045399420161 39.0 37.0 39.0 34.0 39.0 12-13 37.340908861856626 39.0 37.0 39.0 34.0 39.0 14-15 38.480599256220835 40.0 38.0 41.0 34.0 41.0 16-17 38.459896730932684 40.0 38.0 41.0 34.0 41.0 18-19 38.524584353762066 40.0 38.0 41.0 34.0 41.0 20-21 38.453129146804 40.0 38.0 41.0 34.0 41.0 22-23 38.36733250691204 40.0 38.0 41.0 34.0 41.0 24-25 38.368459597741115 40.0 38.0 41.0 34.0 41.0 26-27 38.17520803308373 40.0 37.5 41.0 34.0 41.0 28-29 38.02213028565286 40.0 37.0 41.0 33.0 41.0 30-31 37.94459953841347 40.0 37.0 41.0 33.0 41.0 32-33 37.739887257322735 40.0 36.0 41.0 33.0 41.0 34-35 37.56547541060708 40.0 36.0 41.0 33.0 41.0 36-37 37.35581132062795 40.0 36.0 41.0 33.0 41.0 38-39 37.18583560935133 40.0 35.0 41.0 33.0 41.0 40-41 36.95606697371913 39.0 35.0 41.0 32.0 41.0 42-43 36.69561828742664 39.0 35.0 41.0 31.0 41.0 44-45 36.2886662702532 39.0 35.0 41.0 30.5 41.0 46-47 35.78217583960708 39.0 35.0 41.0 29.5 41.0 48-49 35.383123536545625 39.0 35.0 40.0 27.0 41.0 50-51 35.10236369927671 39.0 35.0 40.0 26.0 41.0 52-53 34.86445850928381 39.0 35.0 40.0 24.5 41.0 54-55 34.635764893220326 38.0 35.0 40.0 24.0 41.0 56-57 34.31923210008432 38.0 34.0 40.0 23.0 41.0 58-59 34.06346646778805 38.0 34.0 40.0 22.0 41.0 60-61 33.73883743352516 38.0 33.5 40.0 19.5 41.0 62-63 33.35736673956643 37.5 33.0 40.0 16.5 41.0 64-65 32.94062868488153 37.0 33.0 40.0 11.0 41.0 66-67 32.51294222777649 36.5 32.0 39.0 7.5 40.5 68-69 32.06343287342652 36.0 31.5 39.0 4.0 40.0 70-71 31.615104360884068 35.5 31.0 39.0 2.0 40.0 72-73 31.123145171314448 35.0 31.0 38.0 2.0 40.0 74-75 30.625740335742048 35.0 30.0 37.5 2.0 39.5 76-77 29.255454884452192 33.5 28.5 36.0 2.0 38.5 78-79 29.876607910128364 34.0 30.0 36.0 2.0 39.0 80-81 29.662351470929117 34.0 30.0 36.0 2.0 38.5 82-83 29.225856572232914 34.0 29.0 36.0 2.0 37.0 84-85 28.768595318961665 34.0 29.0 35.0 2.0 37.0 86-87 28.385470102697965 34.0 29.0 35.0 2.0 36.5 88-89 27.930068297336973 34.0 28.0 35.0 2.0 36.0 90-91 27.378225478635663 33.5 26.5 35.0 2.0 35.5 92-93 26.875553047176563 33.0 26.0 35.0 2.0 35.0 94-95 26.387862021238355 33.0 25.0 35.0 2.0 35.0 96-97 25.763206783373477 32.5 23.5 34.5 2.0 35.0 98-99 24.83877897933611 32.0 11.0 34.0 2.0 35.0 100-101 22.128909963751685 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07499613659115667 1101 2 0.15138812505492183 1101 3 0.043118725537702574 1101 4 0.09818750505321105 1101 5 0.2993688338717533 1101 6 0.2756479480061955 1101 7 0.3312326570660602 1101 8 0.1421935974811248 1101 9 0.062003817304976394 1101 10-11 0.12556514372815997 1101 12-13 0.22263706184725152 1101 14-15 0.1535111765951953 1101 16-17 0.07874007117452919 1101 18-19 -0.012695043189232535 1101 20-21 -0.09730451199455104 1101 22-23 -0.028553304622846554 1101 24-25 0.02801358190944825 1101 26-27 -0.07289075296768033 1101 28-29 0.1176758477556703 1101 30-31 0.3759983264328213 1101 32-33 0.22141272515048627 1101 34-35 0.25648915140145334 1101 36-37 0.6076652944762628 1101 38-39 0.43912750003671874 1101 40-41 0.6113568203782691 1101 42-43 0.8518258723241914 1101 44-45 0.7206491135825601 1101 46-47 0.7315855453505691 1101 48-49 0.701276485690201 1101 50-51 0.5642602693379146 1101 52-53 0.6355398276641679 1101 54-55 0.7102345629877362 1101 56-57 0.9545260227932886 1101 58-59 1.0799471701049086 1101 60-61 1.036995799803293 1101 62-63 0.8246140596959393 1101 64-65 1.0561266760255492 1101 66-67 1.0354857531344592 1101 68-69 0.8830352293399208 1101 70-71 1.1706472456774684 1101 72-73 1.046574768378143 1101 74-75 1.1672725633022552 1101 76-77 1.0895507449172257 1101 78-79 1.3704648320361628 1101 80-81 1.3832780268390579 1101 82-83 1.3491597315499853 1101 84-85 1.362898670442899 1101 86-87 1.40325813565536 1101 88-89 1.2073785539659703 1101 90-91 1.2869976675703256 1101 92-93 1.502697270789259 1101 94-95 1.6584965447474715 1101 96-97 1.703407922156373 1101 98-99 1.6743208895408515 1101 100-101 2.3355161371694457 1102 1 0.07610296410017625 1102 2 0.22455481244869446 1102 3 0.13674721287343772 1102 4 0.13931008516347987 1102 5 0.010047439352000254 1102 6 0.10582661566776608 1102 7 0.0026624129007402075 1102 8 0.0927677383425447 1102 9 0.08634987708195041 1102 10-11 0.10270936293804311 1102 12-13 0.0878507583194903 1102 14-15 0.20197322526244932 1102 16-17 0.32522060842113376 1102 18-19 0.28322171674941643 1102 20-21 0.1128227524418719 1102 22-23 0.1441594591295896 1102 24-25 0.07342252772878766 1102 26-27 -0.152028919862353 1102 28-29 0.2057867792318504 1102 30-31 0.26496321471371687 1102 32-33 0.11933379632737484 1102 34-35 0.25609119094877286 1102 36-37 0.6512606184409435 1102 38-39 0.5790396171034189 1102 40-41 0.5701347501548284 1102 42-43 0.7275046850755089 1102 44-45 0.8119002017556696 1102 46-47 0.87997919456501 1102 48-49 0.834203567728089 1102 50-51 0.8809994808787636 1102 52-53 1.1956691648112852 1102 54-55 1.266089970689933 1102 56-57 1.299275846612879 1102 58-59 1.261893859985932 1102 60-61 1.5178957707853442 1102 62-63 1.5312570974607311 1102 64-65 1.261327107149377 1102 66-67 1.3578088472769778 1102 68-69 1.4515756264712927 1102 70-71 1.5456358457686612 1102 72-73 1.540261291613234 1102 74-75 1.4947463435120163 1102 76-77 1.1708072221381371 1102 78-79 1.0534851860551484 1102 80-81 1.151016284932993 1102 82-83 1.3900854041446031 1102 84-85 1.6305996197443946 1102 86-87 1.6783006262245301 1102 88-89 1.4452075967881726 1102 90-91 2.0375780265638177 1102 92-93 2.0102004834908307 1102 94-95 1.8437554263127822 1102 96-97 2.423187800280985 1102 98-99 2.3194811100772696 1102 100-101 2.884798979270343 1103 1 0.20367612533306456 1103 2 0.055365984141140245 1103 3 0.18448283098849316 1103 4 0.13791918719975627 1103 5 0.0381435782192483 1103 6 0.1096098581290974 1103 7 0.17068288691876887 1103 8 0.18662553293473394 1103 9 0.3335958991137744 1103 10-11 0.21220085064251037 1103 12-13 0.3108395198639471 1103 14-15 0.2558566123771655 1103 16-17 0.4136260829955205 1103 18-19 0.4225896927146948 1103 20-21 0.3723643124730316 1103 22-23 0.19595649929872394 1103 24-25 0.30319887133622814 1103 26-27 0.2731407696892205 1103 28-29 0.25622073939653234 1103 30-31 0.3839962624493296 1103 32-33 0.2671862498713864 1103 34-35 0.17800617926522477 1103 36-37 0.4501645908455245 1103 38-39 0.519815181807985 1103 40-41 0.4111551129010209 1103 42-43 0.14026756479065483 1103 44-45 0.5036493950348557 1103 46-47 0.7701538913492527 1103 48-49 0.8491139938992234 1103 50-51 0.8528755240522372 1103 52-53 0.7013996644218352 1103 54-55 0.6864471532870127 1103 56-57 0.703888064260596 1103 58-59 0.9047947167790795 1103 60-61 1.1176787907030032 1103 62-63 0.9723108417580519 1103 64-65 1.004400435290023 1103 66-67 1.3593388350370859 1103 68-69 0.9094870540889595 1103 70-71 1.1329842378906214 1103 72-73 0.9214786055110835 1103 74-75 1.1059347267322188 1103 76-77 1.1330304334433556 1103 78-79 1.2413955009544466 1103 80-81 1.6495975690099947 1103 82-83 1.3213194088180238 1103 84-85 1.2659618095741507 1103 86-87 1.714408337362837 1103 88-89 1.5869122813325163 1103 90-91 1.3747873288894006 1103 92-93 1.5634440575422381 1103 94-95 1.7470601998745927 1103 96-97 1.8484965796289323 1103 98-99 2.2553885319088067 1103 100-101 2.821846937432131 1104 1 0.13164575295944658 1104 2 0.1610322000762352 1104 3 0.29485572468519194 1104 4 0.21658051443087345 1104 5 0.05960966933637479 1104 6 0.12242970418692778 1104 7 0.04981748230050442 1104 8 0.24194849944191077 1104 9 0.050857184738902106 1104 10-11 0.1884594318782078 1104 12-13 0.06917371998876831 1104 14-15 0.08388734880320925 1104 16-17 0.05855145224869318 1104 18-19 -0.016216995448402827 1104 20-21 -0.09944112751033174 1104 22-23 -0.12741306591866675 1104 24-25 -0.10543697222524884 1104 26-27 0.13209645930852787 1104 28-29 -0.3066368120908791 1104 30-31 -0.05028577416393887 1104 32-33 -0.008297419777051118 1104 34-35 -0.11518604041440028 1104 36-37 0.08277718705938497 1104 38-39 0.2586660763368087 1104 40-41 0.5100993608727364 1104 42-43 0.57118861742223 1104 44-45 0.5476152261145302 1104 46-47 0.7166771907043596 1104 48-49 1.3801234592622365 1104 50-51 0.8344648729184669 1104 52-53 0.412394047613013 1104 54-55 0.8210295331497122 1104 56-57 0.5729137039527075 1104 58-59 0.39341157760703993 1104 60-61 0.544055688976357 1104 62-63 0.7663286803268008 1104 64-65 0.5397850306717586 1104 66-67 0.9526532723160486 1104 68-69 0.8742649821105921 1104 70-71 0.7208443897524894 1104 72-73 0.7508160301002249 1104 74-75 0.7013944729356751 1104 76-77 0.7724365079180799 1104 78-79 0.8481928797769989 1104 80-81 0.7860327156711122 1104 82-83 0.707988589179994 1104 84-85 0.9459922339552591 1104 86-87 0.6838474365931297 1104 88-89 1.0957470535397107 1104 90-91 1.0579642063597525 1104 92-93 1.3734346961942059 1104 94-95 1.4191529812351114 1104 96-97 1.2140377175527917 1104 98-99 1.1687635579125484 1104 100-101 2.0294153272802724 1105 1 -0.012909305959691864 1105 2 -0.03772677324084128 1105 3 -0.0801358099213374 1105 4 0.10881605553882423 1105 5 0.15080735320471916 1105 6 -0.09166009670701669 1105 7 -0.21538636437428949 1105 8 -0.2660343666017013 1105 9 -0.09161538738624841 1105 10-11 -0.17343613902793464 1105 12-13 -0.015048479659071745 1105 14-15 -0.13988262952538122 1105 16-17 0.029747950961954928 1105 18-19 0.1512411855078568 1105 20-21 0.1602729114982111 1105 22-23 -0.06884100848943575 1105 24-25 -0.06002013625070646 1105 26-27 -0.1988665638527891 1105 28-29 0.06352521554016022 1105 30-31 0.01077934164263894 1105 32-33 -0.15444397266882248 1105 34-35 0.07655512171481149 1105 36-37 0.21575582040625818 1105 38-39 0.2635548410665933 1105 40-41 0.07404694315635396 1105 42-43 -0.27012758195471775 1105 44-45 0.16516991255635816 1105 46-47 0.2309075171299284 1105 48-49 0.5953391574878921 1105 50-51 1.0309635861786646 1105 52-53 1.200319010365284 1105 54-55 1.1075407751922484 1105 56-57 0.7638718171858088 1105 58-59 0.9825310073781353 1105 60-61 0.9508220779781169 1105 62-63 1.1662211698297185 1105 64-65 1.0295461372679924 1105 66-67 1.099367006570418 1105 68-69 0.7850143387406909 1105 70-71 0.7475484734582984 1105 72-73 0.3664025125599295 1105 74-75 0.7248774744255826 1105 76-77 0.6314877066177971 1105 78-79 0.7437468551257709 1105 80-81 0.6696716497087678 1105 82-83 0.9521009080205722 1105 84-85 1.045828851861259 1105 86-87 1.0275297134453574 1105 88-89 1.16896000897788 1105 90-91 1.1266989686515068 1105 92-93 0.7506356984967901 1105 94-95 1.0962828742325357 1105 96-97 1.2134409073130534 1105 98-99 1.392169524321865 1105 100-101 1.7700314386171243 1106 1 -0.06618033399707812 1106 2 -0.08541428821807528 1106 3 -0.04632320508069654 1106 4 -0.09487433506745191 1106 5 0.11399930144339265 1106 6 0.03371732960498974 1106 7 -0.050516965860033736 1106 8 0.09867926867268295 1106 9 0.04993745229743496 1106 10-11 -0.1219084610983856 1106 12-13 -0.38780788536240607 1106 14-15 0.0746273153583914 1106 16-17 -0.013793865477062184 1106 18-19 0.12105307144123856 1106 20-21 0.25446773536592815 1106 22-23 0.44980265314487866 1106 24-25 0.331143295333284 1106 26-27 0.021226894091142867 1106 28-29 0.22231385680544236 1106 30-31 0.10261974423070797 1106 32-33 0.04128452002228755 1106 34-35 -0.17563754543112964 1106 36-37 0.36682819040386505 1106 38-39 0.32110336730670497 1106 40-41 0.6906177555215649 1106 42-43 0.5336886174222357 1106 44-45 0.08659933982690404 1106 46-47 0.38720394655720014 1106 48-49 0.6324227903659221 1106 50-51 0.6468394548582737 1106 52-53 0.8384810041347563 1106 54-55 1.0220746836513825 1106 56-57 0.9782439715112403 1106 58-59 0.7851130826237593 1106 60-61 0.5845448194111356 1106 62-63 0.7748780114304807 1106 64-65 0.3370467363573795 1106 66-67 0.1710061151254152 1106 68-69 0.21795227308049192 1106 70-71 0.984410443264192 1106 72-73 1.2802474682273157 1106 74-75 0.4883509946748035 1106 76-77 0.6131555714632313 1106 78-79 0.6965206389743237 1106 80-81 0.8715887357380048 1106 82-83 0.6412034721231414 1106 84-85 0.7686301938214797 1106 86-87 0.793567336258679 1106 88-89 0.9338114348106146 1106 90-91 0.47391320301527173 1106 92-93 0.30345141860222924 1106 94-95 0.15853425213812145 1106 96-97 0.7065753429708543 1106 98-99 0.514102186675089 1106 100-101 0.7079897419959913 1107 1 0.14122707341033092 1107 2 0.2598848570810688 1107 3 0.043990185232694046 1107 4 0.11452737433425852 1107 5 0.04533833278242838 1107 6 0.010070605958269141 1107 7 -0.13085884620191024 1107 8 -0.02425520426179162 1107 9 0.19381209617207418 1107 10-11 0.029159914969994816 1107 12-13 0.03690721435269495 1107 14-15 -0.17252368179260458 1107 16-17 -0.34228389396708536 1107 18-19 -0.04297826759010093 1107 20-21 -0.16996246406426962 1107 22-23 -0.16145090810868368 1107 24-25 -0.2962783570883758 1107 26-27 -0.25840273553849613 1107 28-29 0.0625560220476089 1107 30-31 0.13808982970078887 1107 32-33 0.062153465891228166 1107 34-35 0.4840490642554798 1107 36-37 -0.10475300617732586 1107 38-39 -0.15553480933147057 1107 40-41 0.11996248486628502 1107 42-43 0.25313306186667006 1107 44-45 0.03524606347362891 1107 46-47 0.2126312969845543 1107 48-49 0.38755099549412364 1107 50-51 0.08615569417451496 1107 52-53 0.43848100413475777 1107 54-55 0.4018467634234639 1107 56-57 0.5211926894599586 1107 58-59 0.4152412877519609 1107 60-61 0.3484337083000213 1107 62-63 0.5235247350772028 1107 64-65 0.5991549984656359 1107 66-67 0.8195816137009118 1107 68-69 0.4283511334793566 1107 70-71 0.6567038905576403 1107 72-73 0.25866627164612055 1107 74-75 0.6818695131933232 1107 76-77 0.8614461697538296 1107 78-79 0.7568482743019587 1107 80-81 0.6809904451397131 1107 82-83 0.5595083154279799 1107 84-85 0.7442712194625045 1107 86-87 1.0511884188797644 1107 88-89 0.8689253949245739 1107 90-91 0.8862351403372095 1107 92-93 1.2493203644711777 1107 94-95 0.9660128846167524 1107 96-97 0.6032989896945011 1107 98-99 1.0855409331138368 1107 100-101 1.8134028474090975 1108 1 -0.18695955477629767 1108 2 -0.55627675908055 1108 3 -0.14725560601309695 1108 4 0.05897181877870139 1108 5 0.08656007400416854 1108 6 -0.012969317081655163 1108 7 -0.3219122372553045 1108 8 0.001959321952732296 1108 9 -0.12778482542484682 1108 10-11 -0.12466403885395749 1108 12-13 -0.11682053937506254 1108 14-15 -0.3573240665929873 1108 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-0.5354091956839504 2316 88-89 0.12793355562765285 2316 90-91 0.16959986099006485 2316 92-93 -0.13040342711702024 2316 94-95 0.003960153919951637 2316 96-97 0.20116465196694122 2316 98-99 -0.49829459733186354 2316 100-101 0.0773074299577452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 6.0 6 25.0 7 59.0 8 95.0 9 207.0 10 347.0 11 567.0 12 1054.0 13 1403.0 14 1774.0 15 2585.0 16 3471.0 17 4907.0 18 4024.0 19 2602.0 20 2170.0 21 2131.0 22 2288.0 23 2572.0 24 2733.0 25 2960.0 26 3472.0 27 4076.0 28 4850.0 29 5767.0 30 6950.0 31 8680.0 32 11151.0 33 14077.0 34 19168.0 35 27127.0 36 38537.0 37 60039.0 38 51391.0 39 4403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.914900195944654 23.263974080186063 16.522536995896253 20.298588727973033 2 14.631737371837081 24.14535657175108 40.32008815190078 20.902817904511068 3 17.47511497670231 28.610973934134893 32.816652053119405 21.09725903604339 4 11.306518313966183 20.466356926653432 40.518831319351364 27.708293440029024 5 10.858406009379577 40.31504898074365 36.309243855570635 12.517301154306141 6 26.25802485310865 41.80751102734917 18.508141593514946 13.426322526027231 7 23.907763321004204 36.40755335624469 23.573835367471922 16.110847955279187 8 23.839230823498582 36.643049830516446 21.985493954694643 17.532225391290325 9 25.20282595769126 17.357534711374043 23.43710631607591 34.00253301485879 10-11 22.226701470425205 29.301002119804213 29.739744481286262 18.732551928484323 12-13 23.383840080631614 28.281034772383673 29.862926255669407 18.4721988913153 14-15 20.745022155481426 29.476700630566167 29.208449653810103 20.569827560142308 16-17 17.856243008173507 32.777514621945855 30.193604305453377 19.172638064427268 18-19 17.73849477103763 30.696511897443134 34.11843356211094 17.446559769408303 20-21 18.72085679347461 29.73211766128707 34.198167085477785 17.34885845976054 22-23 18.607432491450147 29.70628153702472 34.076280126047315 17.610005845477815 24-25 18.407862424370695 30.159338056700562 34.05578007787173 17.377019441057012 26-27 18.035419427772823 30.29397486742491 34.322880184370504 17.347725520431766 28-29 17.803053209431084 30.11533525502765 34.67465580842186 17.406955727119406 30-31 18.51629754675939 30.087765711742126 34.23417067701378 17.161766064484702 32-33 17.737378555975113 30.603726299098323 34.20639773170109 17.452497413225473 34-35 18.081661173988557 30.363927718371748 34.159250711360606 17.39516039627909 36-37 18.524098915238067 30.139013467979538 33.581931608598815 17.754956008183587 38-39 17.75159657203135 30.835928497760932 33.99060701651835 17.421867913689365 40-41 17.946611840668663 30.615045570751402 34.0226896317722 17.41565295680773 42-43 17.933211374124152 30.411687925534324 33.62546427653495 18.029636423806572 44-45 18.353943474127302 30.604799290487083 33.13831806469602 17.902939170689592 46-47 18.606405100967855 30.67568339329927 32.71502910951426 18.00288239621862 48-49 18.506293903631217 30.794271489473207 32.37505417090796 18.324380435987624 50-51 18.622530394498586 30.856253086481967 31.914811418051592 18.606405100967855 52-53 18.512508860512852 31.253506411483894 32.021305544077485 18.21267918392577 54-55 19.012896875388435 30.66291753592077 31.74650366682456 18.577681921866233 56-57 18.971743782523543 31.141469215806815 31.064202184305383 18.822584817364255 58-59 18.71844229664493 30.7102855856673 31.736761301983073 18.834510815704693 60-61 18.95494660176236 30.79813484104828 31.538554569001136 18.70836398818822 62-63 18.631600872109626 30.757149719990995 31.858540862501638 18.752708545397738 64-65 18.636136110915142 30.38257258901666 31.926905388199643 19.05438591186855 66-67 18.470851852225124 30.603791459641418 31.30020257399998 19.625154114133483 68-69 18.30808717064928 30.34998605833997 31.70787015107384 19.63405661993691 70-71 18.573146683060713 30.084086686890473 31.7029989686531 19.63976766139571 72-73 18.316348555524016 29.564431574049653 31.792749316773527 20.326470553652808 74-75 18.229140421071726 30.01387447130874 31.489338829370876 20.26764627824866 76-77 17.888325623427363 29.60150368362174 31.95495668007082 20.55521401288008 78-79 17.68860714417692 29.33980360736254 31.639841568991063 21.33174767946948 80-81 18.03362123701158 29.488122713483765 31.206306333544976 21.271949715959675 82-83 18.058877675799884 29.33015977532015 31.199356329870863 21.411606219009098 84-85 17.634520222125918 29.07340032049021 30.971985661926503 22.32009379545737 86-87 17.50014277603647 28.969089827963273 31.040854103047344 22.48991329295291 88-89 17.769401583638203 28.502464146417665 31.2279746967269 22.500159573217232 90-91 17.5435384080406 27.670386491040333 31.75452182925989 23.03155327165917 92-93 17.53439706245475 28.132942063773353 31.39081546780173 22.941845405970167 94-95 17.29706486063379 28.306944962357516 31.09578088413641 23.300209292872285 96-97 17.1279172503687 28.252018181268458 30.745895608881007 23.87416895948184 98-99 17.336793570006805 28.995103597134385 30.10355514122301 23.564547691635802 100-101 17.34869929693997 29.038479307187675 29.918438316351814 23.69438307952054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 666.0 1 458.5 2 309.5 3 357.0 4 293.0 5 318.5 6 609.5 7 1046.5 8 1333.0 9 1366.0 10 1198.0 11 997.0 12 941.0 13 902.0 14 845.0 15 813.0 16 802.5 17 855.5 18 880.0 19 923.0 20 1058.0 21 1181.5 22 1330.5 23 1554.0 24 1869.0 25 2349.5 26 3010.5 27 3745.0 28 4328.5 29 5128.0 30 6179.5 31 7062.0 32 8262.0 33 9644.5 34 11057.0 35 12613.0 36 14243.0 37 15230.5 38 15009.5 39 14175.0 40 13353.5 41 12580.5 42 11596.5 43 11219.5 44 10892.5 45 10184.5 46 9412.0 47 8710.5 48 8482.0 49 7739.5 50 6588.5 51 5479.5 52 4450.5 53 3835.0 54 3296.5 55 2756.0 56 2320.5 57 1973.5 58 1678.0 59 1345.0 60 1069.0 61 873.0 62 673.0 63 500.0 64 381.5 65 312.0 66 262.5 67 228.0 68 191.0 69 149.0 70 119.0 71 91.5 72 68.0 73 59.0 74 54.5 75 38.5 76 24.0 77 18.0 78 15.5 79 12.5 80 7.0 81 4.5 82 4.5 83 2.5 84 1.0 85 1.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04568833167041244 2 3.3594361522362086E-4 3 0.0 4 0.0 5 3.3594361522362086E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006382928689248797 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.3594361522362086E-4 22-23 0.0010078308456708627 24-25 0.0 26-27 0.0025195771141771563 28-29 0.005039154228354313 30-31 5.039154228354313E-4 32-33 3.3594361522362086E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009574393033873195 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.6797180761181043E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.3594361522362086E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009910336649096815 92-93 0.0018476898837299146 94-95 0.0 96-97 0.0 98-99 5.039154228354313E-4 100-101 0.013941660031780266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 297669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.32887367354692 #Duplication Level Percentage of deduplicated Percentage of total 1 88.20690653350579 55.860440409947486 2 6.661085598594038 8.43678096804089 3 1.786136467017322 3.393420320503662 4 0.8077641032955626 2.0461916342252238 5 0.4689008002196634 1.4847479771268066 6 0.2981161548921064 1.1327616187923453 7 0.23603303018466976 1.0463394165944608 8 0.20243494330911038 1.0255981561547427 9 0.13857061605227539 0.7897968934956111 >10 1.0552605610433372 13.284508421989983 >50 0.10316280163182875 4.525071847562059 >100 0.032437788141966774 3.3002682225279587 >500 0.0010635340374415337 0.5044695711578451 >1k 0.0021270680748830674 3.1696045418809335 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3324 1.1166765770033158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2599 0.8731174559661906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2173 0.7300054758809281 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1316 0.44210179763428503 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.2546452603395046 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 740 0.24859827526547942 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 444 0.14915896515928767 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.1380728258569082 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.12530696847841058 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.3594361522362086E-4 0.0 24-25 0.0 0.0 0.0 0.003023492537012588 0.0 26-27 0.0 0.0 0.0 0.0050391542283543134 0.0 28-29 0.0 0.0 0.0 0.012597885570885783 0.0 30-31 0.0 0.0 0.0 0.0273794046407251 0.0 32-33 0.0 0.0 0.0 0.043840641786682524 0.0 34-35 0.0 0.0 0.0 0.05660649916518011 0.0 36-37 0.0 0.0 0.0 0.07625920065576194 0.0 38-39 0.0 0.0 0.0 0.10733398506394687 0.0 40-41 0.0 0.0 0.0 0.14378386731570975 0.0 42-43 0.0 0.0 0.0 0.21366013928222288 0.0 44-45 0.0 0.0 0.0 0.30386099996976507 0.0 46-47 0.0 0.0 0.0 0.39708535319431987 0.0 48-49 0.0 0.0 0.0 0.5255837860173549 0.0 50-51 0.0 0.0 0.0 0.6975869170118487 0.0 52-53 0.0 0.0 0.0 0.9851546516432681 0.0 54-55 0.0 0.0 0.0 1.2517259103232115 0.0 56-57 0.0 0.0 0.0 1.6281507311812784 0.0 58-59 0.0 0.0 0.0 2.1545743762366927 0.0 60-61 0.0 0.0 0.0 2.687548921788967 0.0 62-63 0.0 0.0 0.0 3.3579244059677027 0.0 64-65 0.0 0.0 0.0 3.8781330941414796 0.0 66-67 0.0 0.0 0.0 4.614353526904044 0.0 68-69 0.0 0.0 0.0 5.515858218356631 0.0 70-71 0.0 0.0 0.0 6.37856142225089 0.0 72-73 0.0 0.0 0.0 7.496413801907488 0.0 74-75 0.0 0.0 0.0 8.592933761997386 0.0 76-77 0.0 0.0 0.0 9.484024201378041 0.0 78-79 0.0 0.0 0.0 10.509660058655754 0.0 80-81 0.0 0.0 0.0 11.973534361992684 0.0 82-83 0.0 0.0 0.0 13.242729340307523 0.0 84-85 1.6797180761181043E-4 0.0 0.0 14.886837393211923 0.0 86-87 3.3594361522362086E-4 0.0 0.0 16.576801749594345 0.0 88-89 3.3594361522362086E-4 0.0 0.0 18.655452868790505 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACCG 30 1.2996711E-4 63.33311 5 GGTATCA 1575 0.0 36.504196 1 GGACGTC 70 2.0497334E-4 33.92845 6 GTATCAA 2335 0.0 31.94852 1 GTATAGA 60 0.0039398707 31.677198 1 GACGTGG 75 3.0730732E-4 31.666553 7 TAGGACG 80 4.4858252E-4 29.687393 4 TCGTACC 85 6.3960766E-4 27.941078 9 GTCTAAG 70 0.008368171 27.151886 1 CTGGTCG 105 6.8891066E-5 27.142761 9 CATAAGC 70 0.008381902 27.142761 3 ATCAACG 2930 0.0 25.776358 3 TCAACGC 2955 0.0 25.558285 4 TATCAAC 3000 0.0 25.174911 2 CAACGCA 3020 0.0 25.165472 5 AGGACGT 170 1.1170414E-7 25.14697 5 GTCTAGA 115 1.2757526E-4 24.79085 1 CTATCCC 115 1.2786678E-4 24.78252 4 GGCCTAA 115 1.2786678E-4 24.78252 6 AACGCAG 3070 0.0 24.600891 6 >>END_MODULE