##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937498_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265515 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.829237519537504 33.0 31.0 34.0 30.0 34.0 2 31.928064327815754 33.0 31.0 34.0 30.0 34.0 3 31.93471178652807 33.0 31.0 34.0 30.0 34.0 4 35.58763535016854 37.0 35.0 37.0 33.0 37.0 5 35.60447055721899 37.0 35.0 37.0 35.0 37.0 6 35.668813438035514 37.0 35.0 37.0 35.0 37.0 7 35.62114004858483 37.0 35.0 37.0 33.0 37.0 8 35.629565184641166 37.0 35.0 37.0 33.0 37.0 9 37.2609607743442 39.0 37.0 39.0 34.0 39.0 10-11 37.165009509820536 39.0 37.0 39.0 34.0 39.0 12-13 37.055923394158526 39.0 37.0 39.0 33.5 39.0 14-15 38.229450313541605 40.0 38.0 41.0 33.5 41.0 16-17 38.233662881569785 40.0 38.0 41.0 33.5 41.0 18-19 38.28540948722294 40.0 38.0 41.0 34.0 41.0 20-21 38.27336308683125 40.0 38.0 41.0 34.0 41.0 22-23 38.12671035534716 40.0 38.0 41.0 34.0 41.0 24-25 37.99504171139107 40.0 38.0 41.0 33.0 41.0 26-27 37.83951942451462 40.0 37.0 41.0 33.0 41.0 28-29 37.83240494887295 40.0 37.0 41.0 33.0 41.0 30-31 37.62541852626029 40.0 37.0 41.0 33.0 41.0 32-33 37.35715684612922 39.5 36.0 41.0 33.0 41.0 34-35 37.17579044498427 39.0 36.0 41.0 33.0 41.0 36-37 37.106758563546315 40.0 36.0 41.0 33.0 41.0 38-39 36.978890081539646 40.0 35.5 41.0 32.0 41.0 40-41 36.79087810481517 39.5 35.0 41.0 31.5 41.0 42-43 36.514466226013596 39.0 35.0 41.0 31.0 41.0 44-45 36.20823870591116 39.0 35.0 41.0 30.0 41.0 46-47 35.75212134907632 39.0 35.0 41.0 28.5 41.0 48-49 35.365216654426305 39.0 35.0 41.0 26.5 41.0 50-51 34.44434966009453 38.0 34.0 40.0 24.0 40.5 52-53 34.50550439711504 38.5 35.0 40.0 24.0 40.5 54-55 34.81402557294315 39.0 35.0 40.5 23.5 41.0 56-57 34.63111500291886 39.0 35.0 41.0 22.5 41.0 58-59 34.43261397661149 39.0 35.0 40.0 21.0 41.0 60-61 34.16805265239252 38.5 34.5 40.0 18.5 41.0 62-63 33.85911718735288 38.0 34.0 40.0 14.0 41.0 64-65 33.502423591887464 38.0 34.0 40.0 8.0 41.0 66-67 33.145799672334896 37.5 34.0 40.0 4.0 41.0 68-69 32.69016063122611 37.0 33.0 39.5 2.0 41.0 70-71 32.271920607122006 36.5 33.0 39.0 2.0 41.0 72-73 31.867994275276352 36.0 32.0 39.0 2.0 40.5 74-75 31.405462968193888 35.5 32.0 38.5 2.0 40.0 76-77 30.970421633429375 35.0 31.0 37.5 2.0 39.5 78-79 30.49314163041636 35.0 31.0 37.0 2.0 39.0 80-81 29.983978306310377 35.0 30.5 36.5 2.0 39.0 82-83 29.477268704216335 35.0 30.0 36.0 2.0 38.5 84-85 29.011102950869066 34.0 29.0 36.0 2.0 37.0 86-87 28.560567199593244 34.0 29.0 35.0 2.0 37.0 88-89 28.137058169971567 34.0 29.0 35.0 2.0 36.0 90-91 27.723392275389337 34.0 27.5 35.0 2.0 36.0 92-93 27.27937781292959 34.0 26.5 35.0 2.0 35.5 94-95 26.766195883471745 34.0 25.5 35.0 2.0 35.0 96-97 26.195857107884677 33.0 24.5 35.0 2.0 35.0 98-99 25.502610022032655 33.0 21.0 35.0 2.0 35.0 100-101 23.711837372653147 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2401694302320756 1101 2 -0.010299967301587998 1101 3 0.09255349860676532 1101 4 -0.05169464227058995 1101 5 0.06987509716807949 1101 6 0.16825314078365494 1101 7 0.2630024850854511 1101 8 0.25564190357527394 1101 9 0.09881417128592318 1101 10-11 0.3374143745161362 1101 12-13 0.27687256986499875 1101 14-15 0.16024617297643573 1101 16-17 0.17626572930078765 1101 18-19 0.18081631149471633 1101 20-21 0.26344566954886517 1101 22-23 0.2731312760685469 1101 24-25 0.24080791751867991 1101 26-27 0.4115378879580902 1101 28-29 0.24640259716903046 1101 30-31 0.15824912574663585 1101 32-33 0.28361165354584017 1101 34-35 0.4021908813278472 1101 36-37 0.7246121761356434 1101 38-39 0.7577159740640411 1101 40-41 0.8441172427173242 1101 42-43 1.1128847717554393 1101 44-45 1.3588643277578996 1101 46-47 1.3783010971379142 1101 48-49 1.4486070826257844 1101 50-51 1.1977198042934205 1101 52-53 1.1554996043807293 1101 54-55 1.3808616856084157 1101 56-57 1.621072356052018 1101 58-59 1.5931867725812836 1101 60-61 1.6646748517109344 1101 62-63 1.5472482258295273 1101 64-65 1.4582894522399528 1101 66-67 1.6747051208569914 1101 68-69 1.8372113512862072 1101 70-71 1.7720750073171878 1101 72-73 1.8186194513180105 1101 74-75 1.8057439667159514 1101 76-77 1.7048581573885002 1101 78-79 1.6380619704836796 1101 80-81 1.598334060671533 1101 82-83 1.4013122049150546 1101 84-85 1.2687839858594323 1101 86-87 1.4655809309785361 1101 88-89 1.6489236278910653 1101 90-91 1.7570502239409223 1101 92-93 1.8414524533546412 1101 94-95 2.154199678344554 1101 96-97 1.7148183649282451 1101 98-99 1.6799455990538519 1101 100-101 2.3556642385944677 1102 1 0.1663689049383521 1102 2 0.11658836995283295 1102 3 0.11057849509399631 1102 4 0.0077810007458509745 1102 5 0.22151853153699363 1102 6 0.12454711792004503 1102 7 0.14520050424534503 1102 8 0.14214891649865535 1102 9 0.2826345482147943 1102 10-11 0.316164838381475 1102 12-13 0.2780543005961178 1102 14-15 0.3603153474582541 1102 16-17 0.24228592680834993 1102 18-19 0.527616451284814 1102 20-21 0.13098013969756295 1102 22-23 0.13228734739031722 1102 24-25 0.22914913506161128 1102 26-27 0.19527395848724893 1102 28-29 0.21677105936986152 1102 30-31 0.48128675017964895 1102 32-33 0.6864953647567944 1102 34-35 0.5581685293234031 1102 36-37 0.7466903649171357 1102 38-39 0.6112876471137341 1102 40-41 0.7163047776198184 1102 42-43 0.7238305526524798 1102 44-45 0.5483519682235283 1102 46-47 0.9179557291260707 1102 48-49 1.179365227740874 1102 50-51 1.0445505682030785 1102 52-53 1.4688726169654416 1102 54-55 1.4225447151624877 1102 56-57 1.2036529965644789 1102 58-59 1.4694220955216295 1102 60-61 1.5811574734237936 1102 62-63 1.4113167662119324 1102 64-65 1.3084942735572227 1102 66-67 1.3769720262717016 1102 68-69 1.3789736432673791 1102 70-71 1.3095391140476416 1102 72-73 1.2753493718033901 1102 74-75 1.6224658168451072 1102 76-77 1.567009988170419 1102 78-79 1.3798195787759724 1102 80-81 1.4041231001190013 1102 82-83 1.3940795084433155 1102 84-85 1.4276150812301474 1102 86-87 1.44064208914671 1102 88-89 1.520731063427295 1102 90-91 1.4998472508793377 1102 92-93 1.4172975889834625 1102 94-95 1.568801618832886 1102 96-97 1.7683655386822963 1102 98-99 1.927163307721198 1102 100-101 2.3117168256120024 1103 1 0.1561307628223183 1103 2 0.22016485153012866 1103 3 0.009882063333780167 1103 4 0.1517422826198498 1103 5 0.015093593693869423 1103 6 0.06229866440148868 1103 7 -0.07008894305066349 1103 8 0.12393454738523957 1103 9 0.235377005826777 1103 10-11 0.36862615917507213 1103 12-13 0.04678263726432874 1103 14-15 0.14464128301656842 1103 16-17 0.012955664307142456 1103 18-19 0.2176333469665721 1103 20-21 -0.047475347735989715 1103 22-23 0.12649061755180924 1103 24-25 -0.1490577248599081 1103 26-27 0.13342843721653708 1103 28-29 -0.004903490422030643 1103 30-31 -0.06503672199742994 1103 32-33 0.03432433833268078 1103 34-35 0.07141492697174812 1103 36-37 0.3791154218084145 1103 38-39 0.20812874986604157 1103 40-41 0.3784163257496118 1103 42-43 0.17426988616590933 1103 44-45 0.1690897005391605 1103 46-47 0.5590804595506569 1103 48-49 0.6440811834000044 1103 50-51 0.6920283496947519 1103 52-53 0.45069945683192003 1103 54-55 0.41830019023334586 1103 56-57 0.45307721553984237 1103 58-59 0.8146663503030211 1103 60-61 0.8175001306703962 1103 62-63 0.3701535740727806 1103 64-65 0.7284641452141472 1103 66-67 0.7613258923608299 1103 68-69 1.0134660173167092 1103 70-71 1.3085592560812636 1103 72-73 1.0890206798221875 1103 74-75 1.0381614015318519 1103 76-77 1.2972445588415304 1103 78-79 1.0530048955585585 1103 80-81 1.1405939630808248 1103 82-83 1.0311772057770945 1103 84-85 0.7660896163699604 1103 86-87 1.0973003156037926 1103 88-89 1.0905917312874926 1103 90-91 0.9666236555332048 1103 92-93 0.975022212264701 1103 94-95 0.7036305328846879 1103 96-97 0.8522853368498708 1103 98-99 1.2667046975627265 1103 100-101 1.4530544690993779 1104 1 0.03216400223727689 1104 2 -0.09467589915778163 1104 3 0.14707978073284167 1104 4 0.16084852022784446 1104 5 0.11657486768836378 1104 6 -0.010792897774123844 1104 7 -0.08178754745658523 1104 8 0.05506107355881085 1104 9 0.1311660574708995 1104 10-11 0.2651044947877068 1104 12-13 0.2477295102218804 1104 14-15 0.3463213783629371 1104 16-17 0.13991847592666318 1104 18-19 0.35314218133519404 1104 20-21 0.1896383374342392 1104 22-23 0.24899917181301134 1104 24-25 0.2138818653044794 1104 26-27 0.04181408473154846 1104 28-29 0.11562508877422317 1104 30-31 0.19419446549330388 1104 32-33 0.03385474646568554 1104 34-35 0.30143804956543363 1104 36-37 0.10895180611581168 1104 38-39 0.31531927234878054 1104 40-41 0.5615049770048799 1104 42-43 0.5243492499375861 1104 44-45 0.6702458302234078 1104 46-47 0.43549816014327547 1104 48-49 0.29553417203643306 1104 50-51 0.3871918280797999 1104 52-53 0.4832688108822225 1104 54-55 0.5207838094410207 1104 56-57 0.44256220233085486 1104 58-59 0.2646699202996601 1104 60-61 0.3793269803405863 1104 62-63 0.32444114004905344 1104 64-65 0.29763420337763336 1104 66-67 0.5371685488777231 1104 68-69 0.8538432286550162 1104 70-71 0.9798751568463366 1104 72-73 1.0703578357980952 1104 74-75 0.43271193895365556 1104 76-77 0.583871351391771 1104 78-79 0.6074171368024537 1104 80-81 0.4070447822577492 1104 82-83 0.23092675678136132 1104 84-85 0.19916865609578238 1104 86-87 0.7900224655106989 1104 88-89 0.48096262647788635 1104 90-91 0.7924078190280675 1104 92-93 1.255687110409955 1104 94-95 1.0654826471465526 1104 96-97 0.6297509687667393 1104 98-99 0.6997754988347467 1104 100-101 0.9833582483527579 1105 1 -0.118215273560633 1105 2 0.14478309611916274 1105 3 -0.06548641362811125 1105 4 0.05757816098925872 1105 5 0.09220818670940645 1105 6 0.07900959256507889 1105 7 0.042665007287936874 1105 8 -0.02823647903887405 1105 9 0.17310107965495547 1105 10-11 0.02488694791494339 1105 12-13 0.06182712006873459 1105 14-15 -0.08851832394133652 1105 16-17 -0.1414361827251227 1105 18-19 0.10347852025032722 1105 20-21 0.3159192877705763 1105 22-23 0.021587550230073305 1105 24-25 -0.005232203680776593 1105 26-27 0.12057821812213376 1105 28-29 0.14080757052605009 1105 30-31 0.10583036072731034 1105 32-33 -0.003210517596151874 1105 34-35 0.340217210864985 1105 36-37 0.009874949095632246 1105 38-39 0.42239312378716676 1105 40-41 0.7388199906015558 1105 42-43 0.672055704782295 1105 44-45 0.8989097775541666 1105 46-47 1.0584729705053704 1105 48-49 1.5137465664832632 1105 50-51 1.2861327183045006 1105 52-53 1.2301809333954665 1105 54-55 1.2389747354464689 1105 56-57 1.3802250046780742 1105 58-59 1.2946756452173673 1105 60-61 1.3480438831601091 1105 62-63 1.568669707388402 1105 64-65 1.63344356361808 1105 66-67 1.5936555962514163 1105 68-69 1.5941359150726484 1105 70-71 1.6214559497474461 1105 72-73 1.8559274651096764 1105 74-75 1.9320499953440944 1105 76-77 1.9883081626126113 1105 78-79 1.9486329661999058 1105 80-81 1.8119324307478024 1105 82-83 2.0740997924189735 1105 84-85 2.5257895234208156 1105 86-87 2.1244149086193254 1105 88-89 2.2885302520682664 1105 90-91 2.264502423293134 1105 92-93 2.3682103678881283 1105 94-95 2.772452734451548 1105 96-97 3.053659656329355 1105 98-99 3.3579442407840787 1105 100-101 3.6059358925491196 1106 1 -0.18565153134019496 1106 2 -0.16144055375034227 1106 3 -0.1468756928893029 1106 4 -0.012357594883056322 1106 5 0.07208170608302567 1106 6 0.004054764217357842 1106 7 -0.1397582009031879 1106 8 0.09001286848170764 1106 9 -0.05777625421374921 1106 10-11 -0.6054684024163137 1106 12-13 -0.4824009465845407 1106 14-15 -0.524239062748812 1106 16-17 -0.7743462409463646 1106 18-19 -0.5672301727831979 1106 20-21 -0.2656858116070566 1106 22-23 -0.7457734214623528 1106 24-25 -0.2612350143613611 1106 26-27 -0.2862627776618467 1106 28-29 -0.28769072298930354 1106 30-31 -0.36883336141683287 1106 32-33 -0.2338017643337551 1106 34-35 -0.2115495026463563 1106 36-37 -0.508745809788131 1106 38-39 -0.5146426120088634 1106 40-41 -0.7401400575815344 1106 42-43 -0.29433156684990536 1106 44-45 -0.13954133667992608 1106 46-47 0.17759061756419925 1106 48-49 -0.05620926567895168 1106 50-51 -0.11131759287798815 1106 52-53 0.03473323213065527 1106 54-55 0.06581673504494034 1106 56-57 -0.13941964499067439 1106 58-59 0.32871499474557453 1106 60-61 0.19139361213019157 1106 62-63 0.3005960274044668 1106 64-65 0.09582059634242768 1106 66-67 0.5820063290359982 1106 68-69 -0.03119534371072774 1106 70-71 0.049346935493279176 1106 72-73 0.17748438139154388 1106 74-75 -0.0085492819094668 1106 76-77 0.30754124833836727 1106 78-79 0.24119971986382893 1106 80-81 0.5833939846526413 1106 82-83 0.4689502240592027 1106 84-85 0.16405493197331467 1106 86-87 0.24814508328304896 1106 88-89 0.3050078670050276 1106 90-91 0.20335506332525455 1106 92-93 -0.04758967226445421 1106 94-95 0.522442696306598 1106 96-97 0.8441535330609362 1106 98-99 1.1154477541348093 1106 100-101 1.4207622326414722 1107 1 0.0875824412087276 1107 2 0.21444031152331178 1107 3 0.3421733409211889 1107 4 0.02264375557241749 1107 5 0.05274563910042929 1107 6 0.18182381177794582 1107 7 -0.04618391357536922 1107 8 0.11734117648376952 1107 9 0.26133637377260044 1107 10-11 0.280853939027935 1107 12-13 0.18237303627694956 1107 14-15 0.4662954720806667 1107 16-17 0.4552311892596421 1107 18-19 0.23809553252286975 1107 20-21 0.10763532670413412 1107 22-23 0.3393298269968952 1107 24-25 0.2635151513763532 1107 26-27 0.15531160788091825 1107 28-29 0.1685054122526637 1107 30-31 -0.008071458993384795 1107 32-33 0.1991910905291192 1107 34-35 0.07411479867709403 1107 36-37 0.24167774215529647 1107 38-39 0.47809562061323163 1107 40-41 0.4566151662257667 1107 42-43 0.2651682735508061 1107 44-45 0.0055221347272436105 1107 46-47 -0.11017916660846083 1107 48-49 0.5188883354210532 1107 50-51 0.3414226040429682 1107 52-53 0.005858038769986251 1107 54-55 0.1351871772577269 1107 56-57 0.08576880493198047 1107 58-59 0.04696087585639219 1107 60-61 0.023636350362814085 1107 62-63 -0.07397735143512563 1107 64-65 0.3656907070797786 1107 66-67 0.11763993818963314 1107 68-69 0.380392791924109 1107 70-71 0.4751271304499376 1107 72-73 0.32737266189342407 1107 74-75 0.33868351206013614 1107 76-77 0.3467719797941555 1107 78-79 0.5166926361110349 1107 80-81 0.5172646355721788 1107 82-83 0.21766729136099272 1107 84-85 0.4258530020496778 1107 86-87 0.6691250228808592 1107 88-89 0.408600078560017 1107 90-91 0.337301830825929 1107 92-93 0.5801020310282006 1107 94-95 0.12702319572958132 1107 96-97 0.5707354074931281 1107 98-99 0.46760357214252224 1107 100-101 1.20356602373829 1108 1 0.0868106448527115 1108 2 0.07759806118564683 1108 3 -0.2374043762154443 1108 4 0.1581077981314678 1108 5 0.04730171837494623 1108 6 0.054229335634722986 1108 7 -0.047948019531986574 1108 8 -0.0226020413557535 1108 9 0.178960894845396 1108 10-11 -0.14270376653175987 1108 12-13 0.14846223388438062 1108 14-15 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2316 100-101 -3.50053469568617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1251.0 3 231.0 4 150.0 5 156.0 6 172.0 7 173.0 8 233.0 9 289.0 10 388.0 11 562.0 12 979.0 13 1412.0 14 1608.0 15 2142.0 16 2869.0 17 4056.0 18 3317.0 19 1977.0 20 1924.0 21 1949.0 22 2042.0 23 2326.0 24 2430.0 25 2566.0 26 2811.0 27 3149.0 28 3658.0 29 4210.0 30 5154.0 31 6276.0 32 8170.0 33 10635.0 34 14791.0 35 22212.0 36 31072.0 37 50582.0 38 56869.0 39 10724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.0744435627102 22.829233269092693 15.902203075992896 21.194120092204212 2 13.872426258116425 23.232895546968546 41.37213351917535 21.522544675739685 3 16.501542959233394 28.472791415329873 32.67258669466782 22.35307893076891 4 10.582875964629315 19.492027997386106 41.358092309784354 28.56700372820023 5 10.431028295793215 39.839237890903874 36.68027755638572 13.04945625691719 6 25.400144958173527 41.730211699332585 19.102769305106758 13.766874037387128 7 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34.29223873555084 17.94819532908705 30-31 17.778964732350133 30.568630929687412 33.78926229724782 17.86314204071464 32-33 17.125332628758 30.92211297110612 33.935871062713495 18.016683337422386 34-35 17.626330402154693 30.73849469915311 33.69420993127868 17.94096496741352 36-37 17.701416211422156 30.725655033164443 33.08556709785162 18.487361657561777 38-39 17.13403104607848 31.23988058619948 33.47907805299648 18.147010314725563 40-41 17.447749590525863 30.894540596435878 33.59857948327006 18.0591303297682 42-43 17.344520180861583 30.869320712274583 33.05856865720087 18.72759044966296 44-45 17.646070840182148 31.033766939612796 32.7386265632755 18.581535656929553 46-47 17.847949331541717 31.147287289856497 32.31020087718636 18.694562501415426 48-49 17.703813451357497 31.03230250927175 32.35271358064303 18.911170458727717 50-51 17.68032024218221 31.060736172003722 32.12330305426641 19.13564053154767 52-53 17.75164459713269 31.28877898690929 32.123677479637195 18.83589893632082 54-55 18.16145275013966 30.834138571407006 31.970090438301145 19.03431824015219 56-57 18.092565825971114 31.304556210114765 31.35965491604995 19.24322304786417 58-59 18.144731404763746 30.43167327407111 32.05330203543279 19.370293285732345 60-61 18.133016842208544 30.687688922936424 31.828490993965787 19.350803240889245 62-63 17.934168291104246 30.543074800868798 32.25274424590557 19.270012662121385 64-65 18.047677810609578 30.003736032333663 32.46423409450275 19.48435206255401 66-67 17.684440922298954 30.278689761877807 32.0170572474433 20.019812068379938 68-69 17.869332815097273 29.8847365014915 32.33283585187254 19.91309483153869 70-71 17.962696678404157 29.598660945113092 32.509256009329604 19.92938636715315 72-73 17.700683096199572 29.219722987508018 32.598407366871726 20.481186549420688 74-75 17.79350297678017 29.31170803966524 32.26301338843443 20.631775595120157 76-77 17.481766631756727 29.19792049968392 32.63683281911933 20.683480049440025 78-79 17.342544647574766 28.7274875077369 32.25306079316436 21.67690705152398 80-81 17.669936990132417 28.9113200746532 32.09125202626071 21.327490908953667 82-83 17.758534799640717 28.63186576796214 32.09748881022289 21.51211062217425 84-85 17.642963343119572 28.05134378372667 32.055713628027384 22.24997924512638 86-87 17.699535831540814 28.365598701837808 31.96573455602098 21.969130910600402 88-89 17.91500706607773 27.43751379724791 32.10603078885352 22.541448347820836 90-91 18.064366393315797 26.80832355283169 32.78097085473408 22.346339199118432 92-93 18.42812343153867 27.024706685937904 32.31775658612244 22.22941329640098 94-95 18.03857416715724 27.29022324865281 32.050483780887255 22.62071880330269 96-97 18.200977737693844 27.13608893601686 31.71194662631439 22.950986699974905 98-99 18.670760080534833 28.112280807688173 31.241213909807776 21.975745201969215 100-101 19.175399338115707 28.142346632654213 30.45063980890638 22.2316142203237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 751.0 1 491.0 2 289.0 3 346.5 4 297.5 5 302.0 6 539.5 7 1017.0 8 1450.5 9 1466.0 10 1150.0 11 903.5 12 872.5 13 881.5 14 810.5 15 749.5 16 740.0 17 757.0 18 778.5 19 846.0 20 955.5 21 1080.5 22 1211.0 23 1406.5 24 1691.0 25 2029.5 26 2667.5 27 3429.0 28 3882.5 29 4433.0 30 5308.0 31 6291.5 32 7606.5 33 8827.0 34 10001.5 35 11160.5 36 12524.5 37 13658.0 38 13351.5 39 12519.5 40 11870.5 41 11213.5 42 10348.5 43 9980.5 44 9714.0 45 8976.0 46 8138.0 47 7473.5 48 7339.5 49 6765.0 50 5674.5 51 4666.5 52 3911.5 53 3438.0 54 3006.0 55 2568.0 56 2174.0 57 1838.5 58 1500.0 59 1177.0 60 962.0 61 786.0 62 587.0 63 436.5 64 351.5 65 309.5 66 259.0 67 201.5 68 145.0 69 130.0 70 114.5 71 81.5 72 61.5 73 43.0 74 42.5 75 33.5 76 23.5 77 21.5 78 13.5 79 8.5 80 6.0 81 4.0 82 2.5 83 3.0 84 3.5 85 1.5 86 0.5 87 1.5 88 2.5 89 1.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3333145020055364 2 0.2915089542963675 3 0.28774268873698283 4 0.2922622074082443 5 0.2922622074082443 6 0.23011882567839861 7 0.21354725721710638 8 0.22032653522399864 9 0.21279400410522945 10-11 0.2357682240174755 12-13 0.22616424684104475 14-15 0.2193849688341525 16-17 0.2212681016138448 18-19 0.21524207671882947 20-21 0.2092160518238141 22-23 0.21335894393913712 24-25 0.20469653315255257 26-27 0.2022484605389526 28-29 0.2175018360544602 30-31 0.22522268045119861 32-33 0.21844340244430632 34-35 0.2276707530647986 36-37 0.2062030393763064 38-39 0.20865111198990643 40-41 0.20375496676270644 42-43 0.2112874978814756 44-45 0.21335894393913712 46-47 0.217313522776491 48-49 0.22503436717322942 50-51 0.2223979812816602 52-53 0.23821629663107546 54-55 0.2195732821121217 56-57 0.20187183398301414 58-59 0.20130689414910646 60-61 0.19810556842362956 62-63 0.20770954560006025 64-65 0.1988588215355065 66-67 0.1977289418676911 68-69 0.1933977364743988 70-71 0.2078978588780295 72-73 0.20526147298646027 74-75 0.2062030393763064 76-77 0.2062030393763064 78-79 0.20752123232209105 80-81 0.20959267837975257 82-83 0.20375496676270644 84-85 0.1947159294201834 86-87 0.1977289418676911 88-89 0.19452761614221417 90-91 0.20036532775926033 92-93 0.19810556842362956 94-95 0.19546918253206033 96-97 0.19415098958627575 98-99 0.20206014726098337 100-101 0.19358604975236804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.99570959212839 #Duplication Level Percentage of deduplicated Percentage of total 1 89.0182163754257 57.858021399464164 2 6.143421403644166 7.985920669066441 3 1.648261358324561 3.2138974973277077 4 0.7346276985567849 1.9099059421492173 5 0.4504325525348603 1.463809168769845 6 0.2923667852826631 1.1401552002369677 7 0.2275107026057403 1.0351053688964649 8 0.170688281275082 0.8875204768427805 9 0.13861603661802233 0.8108502894753132 >10 1.0622785531071492 13.604135405075409 >50 0.08402620729930502 3.776612823287271 >100 0.026077098817025694 2.7351912159880483 >500 0.0011589821696455864 0.42297159674395185 >1k 0.002317964339291173 3.1559029466763775 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3036 1.1434382238291623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2220 0.8361109541833796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.7133306969474418 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1229 0.4628740372483664 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 613 0.2308720787902755 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.1920795435286142 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 405 0.15253375515507597 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 400 0.1506506223753837 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.14161158503286067 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 335 0.12616989623938382 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8831327796922962E-4 0.0 22-23 0.0 0.0 0.0 9.415663898461481E-4 0.0 24-25 0.0 0.0 0.0 0.003013012447507674 0.0 26-27 0.0 0.0 0.0 0.00489614522719997 0.0 28-29 0.0 0.0 0.0 0.011675423234092236 0.0 30-31 0.0 0.0 0.0 0.025233979247876768 0.0 32-33 0.0 0.0 0.0 0.04463024687870742 0.0 34-35 0.0 0.0 0.0 0.06515639417735344 0.0 36-37 0.0 0.0 0.0 0.08737736097772253 0.0 38-39 0.0 0.0 0.0 0.11524772611716852 0.0 40-41 0.0 0.0 0.0 0.14782592320584526 0.0 42-43 0.0 0.0 0.0 0.214865450162891 0.0 44-45 0.0 0.0 0.0 0.29414534018793664 0.0 46-47 0.0 0.0 0.0 0.3813343878876899 0.0 48-49 0.0 0.0 0.0 0.507692597405043 0.0 50-51 0.0 0.0 0.0 0.6839538255842419 0.0 52-53 0.0 0.0 0.0 0.974332900212794 0.0 54-55 0.0 0.0 0.0 1.2159388358473158 0.0 56-57 0.0 0.0 0.0 1.6187409374234978 0.0 58-59 0.0 0.0 0.0 2.1435700431237406 0.0 60-61 0.0 0.0 0.0 2.682710957949645 0.0 62-63 0.0 0.0 0.0 3.370242735815302 0.0 64-65 0.0 0.0 0.0 3.8775587066644066 0.0 66-67 0.0 0.0 0.0 4.64907820650434 0.0 68-69 0.0 0.0 0.0 5.563715797600889 0.0 70-71 0.0 0.0 0.0 6.466301338907407 0.0 72-73 0.0 0.0 0.0 7.664727039903584 0.0 74-75 0.0 0.0 0.0 8.79761972016647 0.0 76-77 0.0 0.0 0.0 9.733913338229478 0.0 78-79 0.0 0.0 0.0 10.849857823475134 0.0 80-81 0.0 0.0 0.0 12.404195619833153 0.0 82-83 0.0 0.0 0.0 13.723141818729639 0.0 84-85 0.0 0.0 0.0 15.455247349490612 0.0 86-87 0.0 0.0 0.0 17.243470237086417 0.0 88-89 0.0 0.0 0.0 19.559346929552 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATGG 65 2.5728186E-6 43.868362 1 GGTATCA 1175 0.0 42.87277 1 TATTCCG 60 8.2763945E-5 39.580975 7 TAGAGCA 50 0.0016095629 38.01925 4 CGCAGAA 50 0.0016155427 37.990574 9 GTATCAA 1945 0.0 36.895287 1 GCGCACA 30 0.0039429106 31.676731 52-53 GTATTGA 80 4.4711365E-4 29.702536 1 ATCAACG 2455 0.0 29.230684 3 ACAGTGC 65 0.00583777 29.229029 8 TCAACGC 2465 0.0 29.112103 4 CAACGCA 2520 0.0 28.288132 5 AACGCAG 2560 0.0 28.201445 6 TATCAAC 2545 0.0 28.010254 2 ATAGAGC 70 0.008359451 27.156607 3 CATGGGG 420 0.0 27.156605 4 GTACATG 1090 0.0 27.032034 1 ATGGGAT 255 0.0 26.09164 5 GTATAAA 165 8.3182385E-8 25.922215 1 TACATGG 1075 0.0 25.64089 2 >>END_MODULE