FastQCFastQC Report
Thu 26 May 2016
SRR937498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265515
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31221.1758281076398698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.9494755475208557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.817656252942395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12830.4832118712690432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.2643918422687984No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.2606255767094138No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.17739110784701428No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.14349471781255296No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.138598572585353No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.11298796678153777No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG459.605867E-442.2274179
GGTATCA15200.034.392181
GGCATTC753.0699064E-431.6705597
GTATCAA21550.027.7865051
GTAGTAT700.00835974427.1564251
GACTAGT700.00838282927.1410836
TAGAGTA1109.441846E-525.9122754
CACCTAC1501.0538479E-625.3364477
GCATTCC950.001223193625.0030738
GCTACAC1000.001649128623.752923
CTAGAGT1401.7711156E-523.7529183
GTACATG11300.023.1310231
GAGTACT19600.023.0257912-13
ATCAACG26850.022.1163123
CATGGGT2154.3835826E-822.095744
GTATAGG1302.9233683E-421.9340321
TCAACGC27250.021.791674
TATCAAC27450.021.6328952
CTATACA1100.002869556421.5935634
AGTACAT8650.021.4188182
Produced by FastQC (version 0.11.5)