##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937498_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265515 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.986057284899157 31.0 31.0 34.0 30.0 34.0 2 32.218477298834344 33.0 31.0 34.0 30.0 34.0 3 32.276406229403236 34.0 31.0 34.0 30.0 34.0 4 35.97324444946613 37.0 35.0 37.0 35.0 37.0 5 35.81736248422876 37.0 35.0 37.0 35.0 37.0 6 35.91390693557803 37.0 35.0 37.0 35.0 37.0 7 35.86015102724893 37.0 35.0 37.0 35.0 37.0 8 35.85765399318306 37.0 35.0 37.0 35.0 37.0 9 37.50361373180423 39.0 37.0 39.0 35.0 39.0 10-11 37.434971658851666 39.0 37.0 39.0 34.0 39.0 12-13 37.35874621019528 39.0 37.0 39.0 34.0 39.0 14-15 38.47453439542022 40.0 38.0 41.0 34.0 41.0 16-17 38.48097847579233 40.0 38.0 41.0 34.0 41.0 18-19 38.56607536297385 40.0 38.0 41.0 34.0 41.0 20-21 38.50381711014444 40.0 38.0 41.0 34.0 41.0 22-23 38.37232359753686 40.0 38.0 41.0 34.0 41.0 24-25 38.228842061653765 40.0 37.5 41.0 34.0 41.0 26-27 37.98003126000414 40.0 37.0 41.0 33.0 41.0 28-29 37.91712520949852 40.0 37.0 41.0 33.0 41.0 30-31 37.89537125962752 40.0 37.0 41.0 33.0 41.0 32-33 37.728800632732614 40.0 36.0 41.0 33.0 41.0 34-35 37.57957742500423 40.0 36.0 41.0 33.0 41.0 36-37 37.37224073969456 40.0 36.0 41.0 33.0 41.0 38-39 37.1696777959814 40.0 35.0 41.0 33.0 41.0 40-41 36.96868350187372 39.5 35.0 41.0 32.0 41.0 42-43 36.687089241662434 39.0 35.0 41.0 31.0 41.0 44-45 36.28899497203548 39.0 35.0 41.0 30.5 41.0 46-47 35.80172118336064 39.0 35.0 41.0 29.5 41.0 48-49 35.383328625501385 39.0 35.0 41.0 27.0 41.0 50-51 35.11768638306687 39.0 35.0 40.5 26.0 41.0 52-53 34.860181157373404 39.0 35.0 40.0 24.0 41.0 54-55 34.6331412537898 39.0 35.0 40.0 23.5 41.0 56-57 34.38322317006572 38.0 34.5 40.0 22.5 41.0 58-59 34.13100013181929 38.0 34.0 40.0 21.5 41.0 60-61 33.81235900043312 38.0 34.0 40.0 19.0 41.0 62-63 33.44044404270945 38.0 33.5 40.0 15.0 41.0 64-65 33.09216051823814 37.0 33.0 40.0 9.5 41.0 66-67 32.70139916765531 37.0 33.0 39.5 6.0 41.0 68-69 32.25010074760371 36.0 32.0 39.0 2.0 40.5 70-71 31.869013050110162 36.0 32.0 39.0 2.0 40.0 72-73 31.416982091407267 35.5 31.0 38.5 2.0 40.0 74-75 30.967097904073213 35.0 31.0 38.0 2.0 40.0 76-77 29.566231662994557 34.0 29.5 36.0 2.0 39.0 78-79 30.155211569967797 35.0 30.0 37.0 2.0 39.0 80-81 29.932847485076174 35.0 30.0 36.0 2.0 39.0 82-83 29.5454192795134 34.0 30.0 36.0 2.0 37.5 84-85 29.161725326252753 34.0 30.0 35.5 2.0 37.0 86-87 28.78470331243056 34.0 29.0 35.0 2.0 37.0 88-89 28.376758375233038 34.0 29.0 35.0 2.0 36.0 90-91 27.92577443835565 34.0 29.0 35.0 2.0 36.0 92-93 27.488228536994143 34.0 27.0 35.0 2.0 35.0 94-95 27.06706589081596 33.5 26.0 35.0 2.0 35.0 96-97 26.649281584844545 33.0 25.5 35.0 2.0 35.0 98-99 26.06040336704141 33.0 24.5 35.0 2.0 35.0 100-101 24.590111669773833 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10913113631338334 1101 2 0.1724081989146029 1101 3 0.1971724139287332 1101 4 -0.005940382379840514 1101 5 -0.0819263405928723 1101 6 0.11729649167666878 1101 7 -0.022039858242997923 1101 8 -0.10898155015529909 1101 9 0.07667443207033386 1101 10-11 0.07628994438015013 1101 12-13 0.13622026069748472 1101 14-15 0.0853979763252326 1101 16-17 0.14995122163722385 1101 18-19 0.075417935972105 1101 20-21 -0.042454127812376896 1101 22-23 0.03839379703389767 1101 24-25 0.14006777573611373 1101 26-27 0.16052129554395833 1101 28-29 0.07594034127386351 1101 30-31 0.2801077980834137 1101 32-33 0.308413249558086 1101 34-35 0.32872987874329596 1101 36-37 0.1870472689963023 1101 38-39 0.20683196966449913 1101 40-41 0.13902194449168803 1101 42-43 0.18457884293340499 1101 44-45 0.3058431210631838 1101 46-47 0.4543660024604961 1101 48-49 0.9068750244717378 1101 50-51 0.6952695145342673 1101 52-53 0.7311990186419663 1101 54-55 0.5768678262207771 1101 56-57 0.8346812603591047 1101 58-59 0.7158420886487136 1101 60-61 0.6547624146660596 1101 62-63 0.6551695376016937 1101 64-65 0.8268784409324397 1101 66-67 0.9242771943298678 1101 68-69 0.9258681458598765 1101 70-71 0.6464275443653058 1101 72-73 0.7671452044241072 1101 74-75 1.200051480750556 1101 76-77 1.1990042658622286 1101 78-79 1.241294830866451 1101 80-81 1.0379669130116902 1101 82-83 1.04546355727825 1101 84-85 1.1424661560141054 1101 86-87 1.0439400223896484 1101 88-89 1.061033295298916 1101 90-91 1.2100101082533925 1101 92-93 0.8995739350235503 1101 94-95 0.833977375331628 1101 96-97 1.2825422645339621 1101 98-99 0.9116314587549894 1101 100-101 1.1213336073253828 1102 1 0.08805813922087324 1102 2 0.08849090565443163 1102 3 -0.023468963219904992 1102 4 -0.032003309541266844 1102 5 -0.18616724072581547 1102 6 -0.11698883144381966 1102 7 -0.0011217831670720102 1102 8 0.02535024957016674 1102 9 0.07346790661087965 1102 10-11 -0.0365221063910397 1102 12-13 -0.16091836568465823 1102 14-15 -0.15548234101269998 1102 16-17 -0.13472518950418788 1102 18-19 -0.3085346650964951 1102 20-21 -0.1792070344016068 1102 22-23 -0.24398220230317946 1102 24-25 -0.3045205820544865 1102 26-27 0.06283575841490574 1102 28-29 -0.11177470309507953 1102 30-31 -0.03968727251419324 1102 32-33 0.09651055499585937 1102 34-35 0.013213177789630492 1102 36-37 -0.05110930311989392 1102 38-39 -0.030548190950000276 1102 40-41 0.10724671832355881 1102 42-43 0.3242554522308865 1102 44-45 0.4894617390867353 1102 46-47 0.3342852268416223 1102 48-49 0.12679763552019807 1102 50-51 0.0874582023447914 1102 52-53 -0.043189489747241794 1102 54-55 -0.009832659081446593 1102 56-57 0.25609976957697 1102 58-59 0.3410497021651224 1102 60-61 0.12522094780675985 1102 62-63 0.40487320420950823 1102 64-65 0.5475846691577857 1102 66-67 0.6316475191977347 1102 68-69 0.7985312553248924 1102 70-71 0.5746121825863675 1102 72-73 0.7827058599964367 1102 74-75 0.5985181130701669 1102 76-77 0.7298337471617025 1102 78-79 0.7528617409648426 1102 80-81 0.4820814976585588 1102 82-83 0.6876279259350007 1102 84-85 0.6424805673644833 1102 86-87 0.6301235004970032 1102 88-89 0.7761695366025663 1102 90-91 0.7396812772661079 1102 92-93 1.0709176853613194 1102 94-95 0.9954151237022479 1102 96-97 1.102425604652641 1102 98-99 0.8574898167977842 1102 100-101 0.9636860441045876 1103 1 0.08988964943841538 1103 2 -0.10345594391905166 1103 3 -0.05252587282114263 1103 4 0.015927312851687248 1103 5 -0.1879209117591074 1103 6 -0.025248484647356406 1103 7 0.027541351737390585 1103 8 0.11633509840189049 1103 9 0.008641602461139541 1103 10-11 0.08357917612399746 1103 12-13 0.05967330369112034 1103 14-15 0.05216741928840207 1103 16-17 0.14893682360026617 1103 18-19 -0.027601602216172694 1103 20-21 -0.08615944718737722 1103 22-23 -0.03240156319768772 1103 24-25 0.19821523633295612 1103 26-27 0.29341144786403817 1103 28-29 0.041911898034051376 1103 30-31 0.23579704746895658 1103 32-33 0.2717746358753459 1103 34-35 0.5407270400325856 1103 36-37 0.6635276686611107 1103 38-39 0.7499338625392582 1103 40-41 0.7786600489706714 1103 42-43 0.98055653419555 1103 44-45 0.8381835738936729 1103 46-47 0.7668787826729186 1103 48-49 0.6439153878908641 1103 50-51 0.43073816241787455 1103 52-53 0.44458746893846524 1103 54-55 0.5077223663536543 1103 56-57 0.8809927406986233 1103 58-59 0.7841194964407379 1103 60-61 0.9300712447403541 1103 62-63 1.220066955096648 1103 64-65 1.2114956760763178 1103 66-67 1.0873867854031687 1103 68-69 1.2422741296054518 1103 70-71 1.145505364331708 1103 72-73 1.3363347685537086 1103 74-75 1.5158620569526953 1103 76-77 1.2494354852648968 1103 78-79 1.762071947971286 1103 80-81 1.5898475588584695 1103 82-83 1.6925272157669156 1103 84-85 1.8348509536757796 1103 86-87 1.9123248842029739 1103 88-89 1.6357659633261328 1103 90-91 1.7858032833192468 1103 92-93 2.0695738147392007 1103 94-95 2.0813381361702525 1103 96-97 1.850723444844217 1103 98-99 1.799634698413545 1103 100-101 1.682235579587811 1104 1 -0.13498169337463395 1104 2 0.05595264494365182 1104 3 -0.11843350327706048 1104 4 -0.09238638550007039 1104 5 -0.20912119955576003 1104 6 -0.12095185637188877 1104 7 -0.042425090294102574 1104 8 -0.20817210475289016 1104 9 -0.1729009656296867 1104 10-11 -0.17190900718709656 1104 12-13 -0.30981311109854914 1104 14-15 -0.02744899830234715 1104 16-17 -0.10950347232498814 1104 18-19 -0.13830719832322558 1104 20-21 -0.2709809198933826 1104 22-23 -0.19435308813549312 1104 24-25 -0.11184995729816904 1104 26-27 -0.11254660192917498 1104 28-29 -0.31261073849086785 1104 30-31 -0.2727654521877767 1104 32-33 -0.25718078310495684 1104 34-35 -0.4364963813423586 1104 36-37 -0.0773427514451086 1104 38-39 -0.0781982602905984 1104 40-41 -0.04843402867950175 1104 42-43 -0.09234828969377418 1104 44-45 0.06808311968025293 1104 46-47 0.17089804967022104 1104 48-49 -0.08498730498507001 1104 50-51 0.205073181888082 1104 52-53 0.2706297706526186 1104 54-55 0.2722453873397441 1104 56-57 -0.3059020762154674 1104 58-59 -0.08907422256208264 1104 60-61 0.004928582181932484 1104 62-63 0.1451519484784285 1104 64-65 0.053775970244039684 1104 66-67 -0.1718471482388395 1104 68-69 -0.24368754510743784 1104 70-71 -0.3142799964851086 1104 72-73 -0.22346816763122845 1104 74-75 -0.18515896208911542 1104 76-77 0.05674628917321911 1104 78-79 -0.11776745710250225 1104 80-81 -0.13345573157997848 1104 82-83 -0.06186250703657592 1104 84-85 -0.20111813564554026 1104 86-87 -0.028209956218177012 1104 88-89 -0.17221490149182372 1104 90-91 -0.0061373119364986906 1104 92-93 0.12177815036282524 1104 94-95 0.0038939242332816093 1104 96-97 -0.45243243219411156 1104 98-99 -0.6668238099869335 1104 100-101 -0.2847309667974329 1105 1 -0.12816904130650641 1105 2 0.03737528699317494 1105 3 0.032210549964673874 1105 4 0.10450784664534041 1105 5 0.14760557874679847 1105 6 0.09254463711602057 1105 7 0.10962872393231748 1105 8 0.04157743009754711 1105 9 -0.16628868568120936 1105 10-11 -0.07561946947419784 1105 12-13 -0.011831144589315556 1105 14-15 0.19827019448425887 1105 16-17 0.10761760968445344 1105 18-19 0.0429866330779447 1105 20-21 0.06374428405680987 1105 22-23 -0.005645488307230551 1105 24-25 -0.11405405061432816 1105 26-27 -0.37565308539847564 1105 28-29 -0.24108504792266672 1105 30-31 0.2365804759645087 1105 32-33 -0.03279978983173493 1105 34-35 0.0264991818464253 1105 36-37 0.08220712690039278 1105 38-39 0.14379257984107596 1105 40-41 0.1897010793783238 1105 42-43 0.0558984542592853 1105 44-45 0.4506686356384577 1105 46-47 0.7950357339410132 1105 48-49 0.6136637612808542 1105 50-51 0.8333506972737936 1105 52-53 1.0277537151209089 1105 54-55 1.218159227328293 1105 56-57 0.8419009866155065 1105 58-59 0.9686866190008203 1105 60-61 1.0313061405045332 1105 62-63 1.0249036301730001 1105 64-65 1.1241566772713796 1105 66-67 1.177032914735264 1105 68-69 0.9722635068461862 1105 70-71 1.090028004087383 1105 72-73 1.229612553708403 1105 74-75 1.3464390055650988 1105 76-77 1.2902799946262036 1105 78-79 1.063612248064235 1105 80-81 1.7113918424861438 1105 82-83 1.6456908062595481 1105 84-85 1.5148436505287854 1105 86-87 1.7415839467444698 1105 88-89 1.8924981370082463 1105 90-91 1.477510891870569 1105 92-93 1.479589084955638 1105 94-95 1.7276666450004186 1105 96-97 1.6151537993788097 1105 98-99 1.8206744009762836 1105 100-101 1.5158665714879547 1106 1 -0.3957458331153809 1106 2 0.07439596557177452 1106 3 -0.2333284984191799 1106 4 -0.14144678093948215 1106 5 -0.28892323072921045 1106 6 -0.2481700788044776 1106 7 -0.2680464416970736 1106 8 -0.4448710742188524 1106 9 -0.5813860556872328 1106 10-11 -0.41597783124894505 1106 12-13 -0.5145414437260385 1106 14-15 -0.3843035858811348 1106 16-17 -0.6188579976233228 1106 18-19 -0.1845981892468913 1106 20-21 -0.3007758926145385 1106 22-23 -0.3278699412283359 1106 24-25 -0.30333331180685974 1106 26-27 -0.19115700027501248 1106 28-29 -0.11575880821176554 1106 30-31 -0.0033111120700226593 1106 32-33 -0.11998107872953767 1106 34-35 -0.262368515403125 1106 36-37 -0.06531847036923466 1106 38-39 0.0019234772512319864 1106 40-41 -0.391919077216734 1106 42-43 -0.7919031919964894 1106 44-45 -0.33328538965766796 1106 46-47 -0.25938104983735144 1106 48-49 -0.30380763000604105 1106 50-51 -0.060009069841235885 1106 52-53 -0.18025170547736025 1106 54-55 0.28575609542706815 1106 56-57 0.4039166461216226 1106 58-59 0.27831821908112886 1106 60-61 -0.11814176152316946 1106 62-63 -0.026787797251216716 1106 64-65 -0.21709006033151468 1106 66-67 -0.18381330088409698 1106 68-69 -0.03316712957219892 1106 70-71 0.11086719300527292 1106 72-73 -0.13357254968449084 1106 74-75 -0.1436884788757773 1106 76-77 -0.31202877871248447 1106 78-79 0.09525248094919903 1106 80-81 0.4563526937208344 1106 82-83 0.05002226580582203 1106 84-85 0.3093076135884125 1106 86-87 0.12350524768805471 1106 88-89 -0.1582848382979165 1106 90-91 -0.27148529363434903 1106 92-93 -0.40990599635333425 1106 94-95 -0.3142928008939805 1106 96-97 -0.4454113481819242 1106 98-99 -0.7244872131574276 1106 100-101 -0.6190109631436442 1107 1 -0.02169422116262254 1107 2 0.23403207920646452 1107 3 0.1896587090844406 1107 4 0.03815151994199795 1107 5 -0.06401101033487322 1107 6 -0.07685866377268269 1107 7 -0.027739028924216313 1107 8 0.02253384143906345 1107 9 -0.14123578682382032 1107 10-11 0.088589163619325 1107 12-13 0.08730131414957043 1107 14-15 0.0567551712087635 1107 16-17 -0.3732224241708195 1107 18-19 -0.12832102667660905 1107 20-21 0.06934098316811088 1107 22-23 -0.018658607223869694 1107 24-25 0.17915373609747576 1107 26-27 0.04475529378053267 1107 28-29 0.02044690298719587 1107 30-31 -0.405802088572095 1107 32-33 -0.32395510087701496 1107 34-35 -0.16120712369737333 1107 36-37 -0.10969934791975078 1107 38-39 0.02733045542296253 1107 40-41 0.08701381742318404 1107 42-43 0.42367247034053435 1107 44-45 -0.009710120180407955 1107 46-47 -0.08029223897477067 1107 48-49 -0.6060886720665835 1107 50-51 -0.5682468482192249 1107 52-53 -0.6037422645659234 1107 54-55 -1.2345068750838948 1107 56-57 -0.9030418826185169 1107 58-59 -0.7815990348290569 1107 60-61 -1.0211483664781156 1107 62-63 -0.9454412703771453 1107 64-65 -0.8069690104137699 1107 66-67 -0.6890800772241121 1107 68-69 -0.5630306108008689 1107 70-71 -0.915515796166531 1107 72-73 -0.8381297230584188 1107 74-75 -0.7944729707362121 1107 76-77 -0.843046285526146 1107 78-79 -0.9615464485079208 1107 80-81 -1.4776898333496575 1107 82-83 -1.8363553215440938 1107 84-85 -1.4327124222600425 1107 86-87 -0.7680224857565889 1107 88-89 -0.7204303437365702 1107 90-91 -0.37701725032155764 1107 92-93 -0.21760727560297255 1107 94-95 -0.41600665165637096 1107 96-97 -0.8537522522504766 1107 98-99 -1.267180021368194 1107 100-101 -0.4366196748091262 1108 1 0.13313364068583056 1108 2 0.19013616145790735 1108 3 0.07366874243707144 1108 4 0.028848617436416646 1108 5 -0.009020519968551355 1108 6 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60-61 -0.02908601635821384 2316 62-63 0.04225657134946914 2316 64-65 0.3019998145262761 2316 66-67 0.233797620617608 2316 68-69 0.08363605586579936 2316 70-71 -0.32350140767732327 2316 72-73 -0.12911293648767597 2316 74-75 -0.6858158963956846 2316 76-77 -0.4605043190992859 2316 78-79 -0.21874069311223465 2316 80-81 0.020607528760653082 2316 82-83 0.2975535513575842 2316 84-85 0.4514122779797667 2316 86-87 0.17648590752878235 2316 88-89 -0.16318813750156025 2316 90-91 -0.23895969636472358 2316 92-93 -0.5071756209267129 2316 94-95 -1.0291961000976144 2316 96-97 -1.1574932594447205 2316 98-99 -1.2499138343178338 2316 100-101 -0.6393295069434188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 8.0 6 22.0 7 39.0 8 115.0 9 178.0 10 279.0 11 578.0 12 894.0 13 1304.0 14 1605.0 15 2333.0 16 3294.0 17 4280.0 18 3851.0 19 2337.0 20 1974.0 21 1874.0 22 1946.0 23 2129.0 24 2377.0 25 2584.0 26 2900.0 27 3333.0 28 3925.0 29 4678.0 30 5701.0 31 7046.0 32 8822.0 33 11520.0 34 15252.0 35 22133.0 36 31655.0 37 52005.0 38 55508.0 39 7033.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00105497950325 23.29394743187847 16.59219013744876 20.11280745116952 2 14.71253978117997 24.386569497015234 40.164962431501046 20.735928290303747 3 17.411069054479032 28.59348812684782 32.95633015083894 21.03911266783421 4 11.382784400128052 20.601849236389658 40.34423667212776 27.67112969135454 5 10.856398125687424 40.35422097000196 36.21837850868602 12.571002395624594 6 26.023765135679717 41.80630096228085 18.508935465039638 13.660998436999794 7 23.69357663408847 36.61940003389639 23.577575654859423 16.109447677155718 8 24.018981978419298 36.547840988268085 21.862794945671617 17.570382087640997 9 25.194056832947293 17.311639643711278 23.568159990960964 33.92614353238047 10-11 22.24224620077962 29.44748884243828 29.641828145302522 18.668436811479577 12-13 23.515176397251167 28.295689741218744 29.823216676804982 18.36591718472511 14-15 20.678869367079074 29.625256576841235 29.24260399600776 20.453270060071933 16-17 17.77997476602075 32.67461348699697 30.40412029452197 19.14129145246031 18-19 17.570947027474908 30.675291414797655 34.2319642958025 17.521797261924938 20-21 18.57258535299324 29.666497184716494 34.51330433308853 17.24761312920174 22-23 18.57072373376721 29.60449998493477 34.24929946669077 17.57547681460725 24-25 18.24721437058993 30.15296716375189 34.371380847373686 17.228437618284502 26-27 17.97326664431982 30.29968626535247 34.53585727145015 17.19118981887756 28-29 17.72162148929038 30.043237705689823 34.94250665702481 17.292634147994985 30-31 18.35571758927966 29.9976649065586 34.57990915733257 17.066708346829166 32-33 17.703142948609308 30.51089392313052 34.53966819200422 17.246294936255953 34-35 17.89729393819558 30.4577895787432 34.37376419411333 17.271152288947896 36-37 18.302955205836216 30.165019236237566 33.924135969975275 17.60788958795094 38-39 17.68679300000565 30.812441505077874 34.16329428336306 17.337471211553417 40-41 17.72875355441312 30.573413931416304 34.32762744101087 17.37020507315971 42-43 17.918425230661448 30.434315869168486 33.75746036050798 17.88979853966209 44-45 18.209140726512626 30.66192117206184 33.38154153249346 17.747396568932075 46-47 18.42099316422801 30.781688416850272 32.86217351185432 17.935144907067397 48-49 18.33512230947404 30.71351901022541 32.658418545091614 18.292940135208934 50-51 18.414213886221116 30.839312279908853 32.24224620077962 18.50422763309041 52-53 18.375986290793364 31.174886541250025 32.29196090616349 18.15716626179312 54-55 18.861269608120068 30.797883358755623 31.90177579421125 18.439071238913055 56-57 18.971432875732066 31.08035327570947 31.299361617987685 18.64885223057078 58-59 18.622488371655084 30.78940926124701 31.831723254806697 18.756379112291206 60-61 18.703086454625918 30.890533491516486 31.680696005875376 18.725684047982224 62-63 18.489915823964747 30.757772630548182 32.01702352032842 18.735288025158653 64-65 18.52343558744327 30.353840649304182 32.22284993314879 18.89987383010376 66-67 18.251322900777733 30.559102122290643 31.66431275069205 19.525262226239573 68-69 18.18127036137318 30.322392331883318 31.878236634465097 19.618100672278402 70-71 18.35734327627441 30.041993860987137 32.03095870289815 19.56970415984031 72-73 18.17373783025441 29.445794022936557 32.0544978626443 20.325970284164736 74-75 18.19106265182758 29.88701203321846 31.688040223716175 20.233885091237784 76-77 17.783741031580135 29.47535920757773 32.11005027964522 20.63084948119692 78-79 17.516712803419768 29.30286424495791 31.829463495471067 21.35095945615125 80-81 17.852663691316874 29.39137148560345 31.316309813004917 21.43965501007476 82-83 17.868515655453844 29.146430797564726 31.30450502703242 21.680548519949006 84-85 17.32500988644709 29.074816865337176 31.208217991450578 22.391955256765154 86-87 17.354386757810296 28.775210440088127 31.16113967195827 22.709263130143306 88-89 17.4749072557106 28.39877219742764 31.336082707191682 22.790237839670073 90-91 17.438958935583198 27.412875827456002 32.06922852380907 23.078936713151723 92-93 17.486879049981074 27.933546884209854 31.437363117466166 23.14221094834291 94-95 16.94499369150519 28.319492307402594 31.089957252885903 23.645556748206317 96-97 16.927857183209987 28.24058904393349 30.807863962487996 24.023689810368527 98-99 17.120975016336267 29.236743141120886 30.090936995670653 23.551344846872194 100-101 16.998391598645476 29.387790463347656 29.551643997122206 24.06217394088466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 670.0 1 458.0 2 278.5 3 329.5 4 297.0 5 313.0 6 541.0 7 959.0 8 1262.5 9 1288.5 10 1144.0 11 946.0 12 890.5 13 876.0 14 815.5 15 804.0 16 774.5 17 752.5 18 775.5 19 856.5 20 983.5 21 1108.5 22 1214.5 23 1405.0 24 1727.0 25 2153.5 26 2796.5 27 3522.0 28 3942.0 29 4471.5 30 5457.5 31 6270.5 32 7384.5 33 8625.0 34 9938.5 35 11246.5 36 12401.0 37 13339.0 38 13143.5 39 12484.5 40 11932.5 41 11232.5 42 10346.5 43 9972.0 44 9723.5 45 9077.0 46 8335.5 47 7832.5 48 7700.0 49 6949.0 50 5802.5 51 4774.5 52 3950.0 53 3460.5 54 2918.0 55 2449.5 56 2095.5 57 1724.5 58 1389.5 59 1107.0 60 904.0 61 762.0 62 598.5 63 441.0 64 357.5 65 289.5 66 224.5 67 183.5 68 143.5 69 111.0 70 93.0 71 70.0 72 59.5 73 50.5 74 34.0 75 26.0 76 23.5 77 17.5 78 8.5 79 10.5 80 10.5 81 5.5 82 2.0 83 1.5 84 1.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04029904148541513 2 0.0 3 0.0 4 0.0 5 0.011675423234092236 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007720844396738413 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011298796678153777 24-25 1.8831327796922962E-4 26-27 0.001506506223753837 28-29 0.0022597593356307555 30-31 3.7662655593845924E-4 32-33 0.0 34-35 0.0 36-37 1.8831327796922962E-4 38-39 1.8831327796922962E-4 40-41 0.0 42-43 0.010733856844246088 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8831327796922962E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006590964728923036 92-93 0.0016948195017230665 94-95 0.0 96-97 0.0 98-99 5.649398339076889E-4 100-101 0.012805302901907614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.895302462464734 #Duplication Level Percentage of deduplicated Percentage of total 1 88.48628031776617 56.53857644682109 2 6.485751081300084 8.28818054071853 3 1.6906538208913864 3.2407451173553037 4 0.787455008997133 2.0125870390181877 5 0.4935735484509843 1.5768515582873812 6 0.3346999113809751 1.2831451243108527 7 0.2266856666271116 1.013890446313132 8 0.18134590316177482 0.9269721066280353 9 0.12629940266789574 0.726294468086444 >10 1.0498399638319935 13.01543070924085 >50 0.10556803481324417 4.526511832139667 >100 0.028308746765562685 2.8974649914520394 >500 0.0011795311152317785 0.525304486680211 >1k 0.002359062230463557 3.428045132948264 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3122 1.1758281076398698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2521 0.9494755475208557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 0.817656252942395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1283 0.4832118712690432 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 702 0.2643918422687984 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.2606255767094138 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 471 0.17739110784701428 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.14349471781255296 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.138598572585353 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.11298796678153777 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8831327796922962E-4 0.0 22-23 0.0 0.0 0.0 9.415663898461481E-4 0.0 24-25 0.0 0.0 0.0 0.003013012447507674 0.0 26-27 0.0 0.0 0.0 0.00489614522719997 0.0 28-29 0.0 0.0 0.0 0.011863736512061466 0.0 30-31 0.0 0.0 0.0 0.024669039413969078 0.0 32-33 0.0 0.0 0.0 0.043876993766830505 0.0 34-35 0.0 0.0 0.0 0.0642148277875073 0.0 36-37 0.0 0.0 0.0 0.08700073442178408 0.0 38-39 0.0 0.0 0.0 0.11543603939513775 0.0 40-41 0.0 0.0 0.0 0.1487674895956914 0.0 42-43 0.0 0.0 0.0 0.21863171572227558 0.0 44-45 0.0 0.0 0.0 0.300171365082952 0.0 46-47 0.0 0.0 0.0 0.38811366589458224 0.0 48-49 0.0 0.0 0.0 0.5161666949136583 0.0 50-51 0.0 0.0 0.0 0.6935578027606726 0.0 52-53 0.0 0.0 0.0 0.9856316968909478 0.0 54-55 0.0 0.0 0.0 1.2326987175865771 0.0 56-57 0.0 0.0 0.0 1.6373839519424513 0.0 58-59 0.0 0.0 0.0 2.162777997476602 0.0 60-61 0.0 0.0 0.0 2.7045552981940757 0.0 62-63 0.0 0.0 0.0 3.3941585221173947 0.0 64-65 0.0 0.0 0.0 3.902792685912284 0.0 66-67 0.0 0.0 0.0 4.679396644257387 0.0 68-69 0.0 0.0 0.0 5.6034498992523964 0.0 70-71 0.0 0.0 0.0 6.5131913451217445 0.0 72-73 0.0 0.0 0.0 7.719337890514661 0.0 74-75 0.0 0.0 0.0 8.859951415174283 0.0 76-77 0.0 0.0 0.0 9.799258045684802 0.0 78-79 0.0 0.0 0.0 10.913507711428732 0.0 80-81 0.0 0.0 0.0 12.475754665461462 0.0 82-83 0.0 0.0 0.0 13.805246407924223 0.0 84-85 0.0 0.0 0.0 15.557313146149935 0.0 86-87 0.0 0.0 0.0 17.35758808353577 0.0 88-89 0.0 0.0 0.0 19.69191947724234 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 45 9.605867E-4 42.227417 9 GGTATCA 1520 0.0 34.39218 1 GGCATTC 75 3.0699064E-4 31.670559 7 GTATCAA 2155 0.0 27.786505 1 GTAGTAT 70 0.008359744 27.156425 1 GACTAGT 70 0.008382829 27.141083 6 TAGAGTA 110 9.441846E-5 25.912275 4 CACCTAC 150 1.0538479E-6 25.336447 7 GCATTCC 95 0.0012231936 25.003073 8 GCTACAC 100 0.0016491286 23.75292 3 CTAGAGT 140 1.7711156E-5 23.752918 3 GTACATG 1130 0.0 23.131023 1 GAGTACT 1960 0.0 23.02579 12-13 ATCAACG 2685 0.0 22.116312 3 CATGGGT 215 4.3835826E-8 22.09574 4 GTATAGG 130 2.9233683E-4 21.934032 1 TCAACGC 2725 0.0 21.79167 4 TATCAAC 2745 0.0 21.632895 2 CTATACA 110 0.0028695564 21.593563 4 AGTACAT 865 0.0 21.418818 2 >>END_MODULE