FastQCFastQC Report
Thu 26 May 2016
SRR937497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326661
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35851.097468017302341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.7907280024245319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.7007264411729591No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13220.40470089787271823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.21949360346046817No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.1931666161555864No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4950.15153324088275003No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.14724745225172273No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.14571681345492726No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.1249001258185091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.040.7218131
CATGGGT2100.033.9684074
GTATCAA21700.032.2201461
ACTAAGC650.00583519929.2336488
CCTTAGA856.3477864E-427.978281
GTACATG15300.027.3565391
TAAGGGT908.8727236E-426.419874
CATGGGG6750.026.0676064
ATCAACG27250.025.6539383
CAACGCA27550.025.5511265
TCAACGC27500.025.4207214
TACATGG15750.025.3630772
ACATGGG15150.025.1119563
AACGCAG28150.024.9758176
GTCTTAG1151.2675242E-424.8155171
TATCAAC28300.024.7021122
ATGGGGA4150.024.0680625
GTACTAG1000.001638403623.7815381
AGTACTT20650.022.77289212-13
ACGCAGA30900.022.7530527
Produced by FastQC (version 0.11.5)