FastQCFastQC Report
Thu 26 May 2016
SRR937497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326661
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37731.15502003606185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.9290977496548409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25400.7775645087720909No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14790.4527629560920955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.27735174997933637No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.25653506234291823No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.17326831179724544No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.16500286229455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.12826753117145911No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.11694080407517274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.043.2403141
CATTCCG704.294041E-640.7234739
GCTTCGT500.001612334338.0085758
GTATCAA25050.036.0526351
GTGCTAC801.070211E-535.6494141
GTTTAAG753.0613306E-431.6883681
CGCAGAA600.00394249131.6738119
GTAAGAC600.00394249131.6738113
GTGTAGC1102.7110345E-630.2479881
GTACCAC953.4779143E-530.006773
GGACCCC804.4887958E-429.6851086
GGCTTCG650.00583159529.2373647
ATCAACG31350.028.6428243
CCCTATA1503.3140168E-828.5064322
TCAACGC31700.028.326584
CAACGCA32050.028.1654825
CAATGGG1354.2012107E-728.1545019
TATCAAC32050.028.0172422
AACGCAG32700.027.7424136
ACGCAGC700.00837370927.1489837