##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937497_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 326661 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94297452098659 31.0 31.0 34.0 30.0 34.0 2 32.171342768190875 33.0 31.0 34.0 30.0 34.0 3 32.23007337882392 34.0 31.0 34.0 30.0 34.0 4 35.930906964712655 37.0 35.0 37.0 35.0 37.0 5 35.811394687458865 37.0 35.0 37.0 35.0 37.0 6 35.894355310245174 37.0 35.0 37.0 35.0 37.0 7 35.83883597980781 37.0 35.0 37.0 35.0 37.0 8 35.82628474167409 37.0 35.0 37.0 35.0 37.0 9 37.47476129687964 39.0 37.0 39.0 35.0 39.0 10-11 37.40697236584717 39.0 37.0 39.0 34.0 39.0 12-13 37.341510005785814 39.0 37.0 39.0 34.0 39.0 14-15 38.45750946700096 40.0 38.0 41.0 34.0 41.0 16-17 38.45283030419915 40.0 38.0 41.0 34.0 41.0 18-19 38.527794869911006 40.0 38.0 41.0 34.0 41.0 20-21 38.46292945898041 40.0 38.0 41.0 34.0 41.0 22-23 38.32344999862242 40.0 38.0 41.0 34.0 41.0 24-25 38.189375223855926 40.0 37.5 41.0 33.5 41.0 26-27 38.063784779940065 40.0 37.0 41.0 33.0 41.0 28-29 37.94474393943568 40.0 37.0 41.0 33.0 41.0 30-31 37.8898077823799 40.0 37.0 41.0 33.0 41.0 32-33 37.70584336667065 40.0 36.0 41.0 33.0 41.0 34-35 37.54274768031691 40.0 36.0 41.0 33.0 41.0 36-37 37.33285577402873 40.0 35.5 41.0 33.0 41.0 38-39 37.1540878770346 39.5 35.0 41.0 33.0 41.0 40-41 36.94676285200866 39.0 35.0 41.0 32.0 41.0 42-43 36.67336933395784 39.0 35.0 41.0 31.0 41.0 44-45 36.28005791937207 39.0 35.0 41.0 30.5 41.0 46-47 35.7850125971573 39.0 35.0 41.0 29.0 41.0 48-49 35.39288436636146 39.0 35.0 41.0 27.0 41.0 50-51 35.1429126831792 39.0 35.0 40.0 26.0 41.0 52-53 34.90401517169175 39.0 35.0 40.0 24.5 41.0 54-55 34.65694557966822 38.5 35.0 40.0 24.0 41.0 56-57 34.406947875626415 38.0 34.0 40.0 23.0 41.0 58-59 34.17733062716394 38.0 34.0 40.0 22.0 41.0 60-61 33.86085115762212 38.0 34.0 40.0 20.5 41.0 62-63 33.484771674610684 38.0 33.5 40.0 18.0 41.0 64-65 33.14574130367568 37.0 33.0 40.0 14.0 41.0 66-67 32.73464692754874 37.0 33.0 39.5 8.0 41.0 68-69 32.309120158206824 36.0 32.0 39.0 6.0 40.0 70-71 31.905611627956812 36.0 31.5 39.0 2.0 40.0 72-73 31.43765861244532 35.0 31.0 38.5 2.0 40.0 74-75 30.967536681758766 35.0 31.0 37.5 2.0 40.0 76-77 29.572608912603584 33.5 29.5 36.0 2.0 39.0 78-79 30.19814272288397 34.5 30.0 36.0 2.0 39.0 80-81 30.01764826532705 35.0 30.0 36.0 2.0 39.0 82-83 29.63278138498321 34.0 30.0 36.0 2.0 37.5 84-85 29.22091556690269 34.0 29.5 35.5 2.0 37.0 86-87 28.801419514420147 34.0 29.0 35.0 2.0 36.5 88-89 28.357910800493478 34.0 29.0 35.0 2.0 36.0 90-91 27.897526793832135 34.0 28.5 35.0 2.0 36.0 92-93 27.484808409941806 34.0 27.0 35.0 2.0 35.0 94-95 27.043548204407628 33.0 26.0 35.0 2.0 35.0 96-97 26.563008746070086 33.0 25.5 35.0 2.0 35.0 98-99 26.084756062094954 33.0 25.0 35.0 2.0 35.0 100-101 24.31069977744512 31.0 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0486733215367714 1101 2 0.17565741715141314 1101 3 0.1483060559628413 1101 4 0.15214444657770088 1101 5 0.05574592367264586 1101 6 0.014512013547644642 1101 7 0.08793035556386997 1101 8 -0.014972367743389725 1101 9 0.09432939076655344 1101 10-11 0.18169944118713488 1101 12-13 0.017339894375147935 1101 14-15 0.2028639236414307 1101 16-17 -0.005472160403975579 1101 18-19 0.1603347276506284 1101 20-21 0.15621518997373585 1101 22-23 0.03498605292089252 1101 24-25 0.021447784077565757 1101 26-27 0.15689694236632334 1101 28-29 0.038364175235486186 1101 30-31 0.17935522191914544 1101 32-33 0.19265589170270658 1101 34-35 0.1788268503543904 1101 36-37 0.2666632818752177 1101 38-39 0.2754391049425138 1101 40-41 0.19556737948582992 1101 42-43 0.47913194911956936 1101 44-45 0.6529161344812735 1101 46-47 0.9808670716402403 1101 48-49 0.8894982756225431 1101 50-51 1.073158082799182 1101 52-53 1.2480274751681932 1101 54-55 1.2336509851371105 1101 56-57 1.2657108626984765 1101 58-59 1.298277660064855 1101 60-61 1.2853328821165277 1101 62-63 1.1783147919872619 1101 64-65 1.1239064073549514 1101 66-67 1.3149749478013248 1101 68-69 1.1022275148104121 1101 70-71 0.9294852715081738 1101 72-73 0.9572211235965362 1101 74-75 1.026127301394947 1101 76-77 0.8911095902857333 1101 78-79 0.8941248385350953 1101 80-81 0.9365328482794659 1101 82-83 1.3425751079691466 1101 84-85 1.5297072237703517 1101 86-87 1.7130488475348535 1101 88-89 1.3129036287477724 1101 90-91 1.5458801300871983 1101 92-93 1.313734009639429 1101 94-95 1.1731330359824526 1101 96-97 1.3464356880565163 1101 98-99 1.2623669828988326 1101 100-101 1.4718993205655906 1102 1 0.022336656456651838 1102 2 0.08097946981826709 1102 3 0.12589042621736724 1102 4 0.03670864826979425 1102 5 -0.17898195129456695 1102 6 0.00790680125806631 1102 7 -0.11041380837491488 1102 8 0.03403247931680653 1102 9 0.0948409709340794 1102 10-11 -0.012586078827638403 1102 12-13 0.03622788745909844 1102 14-15 -0.03690690139491437 1102 16-17 0.11353174767996421 1102 18-19 0.005003770898582616 1102 20-21 0.03478127609405135 1102 22-23 0.0052593597686225735 1102 24-25 0.18808045332803403 1102 26-27 0.16153030451651773 1102 28-29 0.0860738826310552 1102 30-31 0.40424651113354315 1102 32-33 0.5327919461244832 1102 34-35 0.5596108631503753 1102 36-37 0.3408116465994979 1102 38-39 0.48355833550003524 1102 40-41 0.5255495389369429 1102 42-43 0.48034290470599217 1102 44-45 0.6257829585074077 1102 46-47 0.6937151808982236 1102 48-49 1.1352218863424142 1102 50-51 1.3653885399510983 1102 52-53 1.4893071379138263 1102 54-55 1.2473470870905032 1102 56-57 1.415200739563069 1102 58-59 1.2650265681002253 1102 60-61 1.2102617303811982 1102 62-63 1.1333928727197886 1102 64-65 1.1688412780190731 1102 66-67 1.2686348506010958 1102 68-69 1.3445238621927764 1102 70-71 1.3779840427444192 1102 72-73 1.6150776026945977 1102 74-75 1.713559913659271 1102 76-77 1.5512552287410166 1102 78-79 1.4462882074523868 1102 80-81 1.3650184738481137 1102 82-83 1.289913110280974 1102 84-85 1.3526357288230137 1102 86-87 1.2794020904026837 1102 88-89 1.216716843705342 1102 90-91 1.2563548959097552 1102 92-93 1.1892710878691055 1102 94-95 1.148713177605977 1102 96-97 1.050243722455427 1102 98-99 1.227911411046101 1102 100-101 1.3979387510926813 1103 1 -0.01511864186821299 1103 2 -0.08026921938073173 1103 3 0.04458043186389915 1103 4 0.03767662439305042 1103 5 -0.11735413811396 1103 6 -0.1333639362859671 1103 7 -0.12883224294241558 1103 8 -0.011731280732668381 1103 9 -0.010480208921258338 1103 10-11 -0.13463173503478032 1103 12-13 -0.11667200767682573 1103 14-15 -0.19332915409046336 1103 16-17 -0.13841092662296006 1103 18-19 -0.1820172825821018 1103 20-21 -0.17935655295060826 1103 22-23 -0.16468767042686494 1103 24-25 -0.20341211207757937 1103 26-27 -0.23710108479810543 1103 28-29 -0.22984932459650054 1103 30-31 -0.29637998452400893 1103 32-33 -0.24626750374242334 1103 34-35 0.14824789899238056 1103 36-37 0.3755377900212906 1103 38-39 0.365371139673087 1103 40-41 0.11022056149617043 1103 42-43 0.5520672466166658 1103 44-45 0.6204456457166785 1103 46-47 0.6100390226879 1103 48-49 0.5140904298072329 1103 50-51 0.7589514987314487 1103 52-53 0.7236380244186265 1103 54-55 0.6217521850980816 1103 56-57 0.7138751500387244 1103 58-59 0.8371273451699466 1103 60-61 0.9100009145924375 1103 62-63 0.9647499214681403 1103 64-65 0.95265994382531 1103 66-67 1.1067811763250646 1103 68-69 1.1589682170097966 1103 70-71 1.065502528399552 1103 72-73 1.1640545057087763 1103 74-75 1.130703846331155 1103 76-77 1.1415458904602502 1103 78-79 1.2978786459993508 1103 80-81 1.6111667355243249 1103 82-83 1.5936021748395994 1103 84-85 1.3286514315467919 1103 86-87 1.317408597353289 1103 88-89 0.9744539306349758 1103 90-91 1.1461803913253128 1103 92-93 1.447962706809168 1103 94-95 1.0672015215601576 1103 96-97 0.41863930203112787 1103 98-99 0.8091676289482805 1103 100-101 0.9478567420044612 1104 1 0.05470916199988807 1104 2 0.15319540719099933 1104 3 0.2784086397111949 1104 4 0.12905671831821763 1104 5 -0.0014626065278378064 1104 6 0.03873625055441465 1104 7 0.1271037263551662 1104 8 0.16898881218254758 1104 9 0.11534416112368007 1104 10-11 0.1573922007325308 1104 12-13 0.23613059798898206 1104 14-15 0.3678534950031178 1104 16-17 0.2628439337517321 1104 18-19 0.347894851942975 1104 20-21 0.14478625369484632 1104 22-23 0.08773367987360103 1104 24-25 0.09554006688520644 1104 26-27 -0.1744464365124898 1104 28-29 0.14945684113597224 1104 30-31 0.5082127539499552 1104 32-33 0.16298109495474478 1104 34-35 0.41136207361238775 1104 36-37 0.28480911254818153 1104 38-39 0.2554054779046737 1104 40-41 0.5544083108270783 1104 42-43 0.6306664487577578 1104 44-45 0.6942475949162912 1104 46-47 0.7081049722915012 1104 48-49 0.6898239045332843 1104 50-51 0.7413784037458413 1104 52-53 0.9362346006949451 1104 54-55 0.9646623502245788 1104 56-57 0.836172276736697 1104 58-59 0.9183751359087182 1104 60-61 1.0143840888589608 1104 62-63 0.8875898952094943 1104 64-65 0.6860185258583442 1104 66-67 0.8207639062144736 1104 68-69 1.241892521408122 1104 70-71 1.3338078356762217 1104 72-73 1.2377302114099251 1104 74-75 1.2102927975001698 1104 76-77 1.1984564595659428 1104 78-79 1.51660813143085 1104 80-81 1.5852388458106432 1104 82-83 1.5643667054662735 1104 84-85 1.7150752057224246 1104 86-87 1.488486211856145 1104 88-89 1.899440043850145 1104 90-91 1.5017755031770506 1104 92-93 1.1788746898420435 1104 94-95 1.2566728137886045 1104 96-97 1.43685623885888 1104 98-99 1.4739406431380502 1104 100-101 1.8583804000360686 1105 1 0.02059399017724317 1105 2 -0.06002831290562938 1105 3 -0.14405687887370533 1105 4 0.07103443202506554 1105 5 -0.01837213664550319 1105 6 0.001013192812649777 1105 7 -0.0438072063963304 1105 8 -0.05767681549609449 1105 9 0.23731875786645418 1105 10-11 -0.12624763406837758 1105 12-13 -0.1785853686595047 1105 14-15 -0.04168441507246712 1105 16-17 0.1463045431182053 1105 18-19 -0.07440312982278385 1105 20-21 -0.15639040024422002 1105 22-23 -0.2782914116872206 1105 24-25 -0.21986942427040645 1105 26-27 -0.18276483439295532 1105 28-29 -0.16281620491717774 1105 30-31 0.19948915073948825 1105 32-33 0.23472191939173825 1105 34-35 -0.040204200836306825 1105 36-37 0.1338077878384496 1105 38-39 0.6358815941674294 1105 40-41 0.49614056361286885 1105 42-43 0.46153218553013886 1105 44-45 0.845225542395454 1105 46-47 0.4266041540884302 1105 48-49 0.6927032000994018 1105 50-51 0.43673412727112293 1105 52-53 0.7047961217734198 1105 54-55 0.44088381052224435 1105 56-57 0.3466647570608643 1105 58-59 0.2955706474804529 1105 60-61 0.550182421283175 1105 62-63 0.5865690894742812 1105 64-65 0.974984473025799 1105 66-67 0.9796901120899477 1105 68-69 1.3894301197872956 1105 70-71 1.1655891806462257 1105 72-73 1.5668816179399698 1105 74-75 0.7470262191474859 1105 76-77 0.7331326849224453 1105 78-79 0.9950621172486649 1105 80-81 0.9864271883649955 1105 82-83 1.1263514323422967 1105 84-85 1.18683940739 1105 86-87 1.2585049136406035 1105 88-89 1.2739432777003756 1105 90-91 1.3643290759971265 1105 92-93 1.2798838069619727 1105 94-95 1.090141215565275 1105 96-97 1.0657232224168958 1105 98-99 1.1529323052591494 1105 100-101 1.1685884573882106 1106 1 -0.21268017856018417 1106 2 -0.44716592975570535 1106 3 -0.2728108724839302 1106 4 -0.15576712992026387 1106 5 -0.4634318928873711 1106 6 -0.3178129816045754 1106 7 -0.38581406948945585 1106 8 -0.15088471988610763 1106 9 -0.4802294612792082 1106 10-11 -0.5243602834589751 1106 12-13 -0.6412406757237576 1106 14-15 -0.7005295221603944 1106 16-17 -0.14758967058431693 1106 18-19 -0.16719096558186664 1106 20-21 -0.3799201600359581 1106 22-23 -0.08972792807580987 1106 24-25 -0.06810040285282071 1106 26-27 -0.12123957111050032 1106 28-29 -0.14074189418471406 1106 30-31 -0.23790287387299003 1106 32-33 -0.03512188607506772 1106 34-35 -0.24410314770648966 1106 36-37 -0.4342965785087287 1106 38-39 -0.11089985181529016 1106 40-41 -0.19640469730300225 1106 42-43 -0.25302822152227833 1106 44-45 0.01606331307347375 1106 46-47 0.10431093435111904 1106 48-49 0.17455741853508755 1106 50-51 -0.15726658270402538 1106 52-53 0.034473083079767264 1106 54-55 -0.30841804724605737 1106 56-57 -0.16721525713863628 1106 58-59 -0.2009563907398757 1106 60-61 0.1329477744958183 1106 62-63 -0.2792574399305252 1106 64-65 -0.007070905035973851 1106 66-67 0.07117041027950677 1106 68-69 0.4125338944885222 1106 70-71 0.277303770635573 1106 72-73 0.8120301210756935 1106 74-75 0.20564058431137155 1106 76-77 0.38969403860840046 1106 78-79 0.16434977551395846 1106 80-81 -0.28955790215683663 1106 82-83 -0.36756644719858755 1106 84-85 -0.47833039500025265 1106 86-87 -0.48590403204629595 1106 88-89 -0.25408299138020496 1106 90-91 -0.26276830892773617 1106 92-93 -0.3307007392455823 1106 94-95 -0.763923392173453 1106 96-97 -0.35031629953316923 1106 98-99 -0.04620389164062999 1106 100-101 -0.19907217448967174 1107 1 -0.27136963724964147 1107 2 -0.1981223807121566 1107 3 -0.1618218614949143 1107 4 0.026528352375216002 1107 5 -0.13359876305423768 1107 6 -0.24707631086790371 1107 7 -0.046335030010418166 1107 8 -0.13261040161179238 1107 9 -0.1648041458538927 1107 10-11 -0.2149789240776201 1107 12-13 -0.3169427514258274 1107 14-15 -0.6770048986357722 1107 16-17 -0.39936012235476426 1107 18-19 -0.44169239008329697 1107 20-21 -0.2373275674433586 1107 22-23 -0.2860445743924558 1107 24-25 -0.2961020308544491 1107 26-27 -0.5708325707035016 1107 28-29 -0.7204529238957491 1107 30-31 -0.530414066384175 1107 32-33 -1.0472708482240307 1107 34-35 -0.6602407138440611 1107 36-37 -0.9062746004867464 1107 38-39 -1.0220313129467513 1107 40-41 -0.4359651368634445 1107 42-43 -0.748856467350528 1107 44-45 -0.575206528196361 1107 46-47 -1.0479479179077344 1107 48-49 -1.1657152717375965 1107 50-51 -1.2676450930825354 1107 52-53 -1.1357344871277988 1107 54-55 -1.3035543923824022 1107 56-57 -0.9594008493242328 1107 58-59 -1.1588114985949858 1107 60-61 -0.7279191363710922 1107 62-63 -0.30034006101314503 1107 64-65 -0.40432365228871703 1107 66-67 -0.40611896700986705 1107 68-69 -0.42456419260956935 1107 70-71 -0.6212310278992241 1107 72-73 -0.5580349989894273 1107 74-75 -0.08851895984817304 1107 76-77 -0.523289274374914 1107 78-79 -0.5678643067001268 1107 80-81 -0.3076897702887038 1107 82-83 -0.36992704955918754 1107 84-85 -0.4606462273160865 1107 86-87 -0.45843150457377035 1107 88-89 -0.410798498095712 1107 90-91 -0.7753039214633475 1107 92-93 -0.5350759936208327 1107 94-95 -0.37649970474976513 1107 96-97 -0.6038165030333715 1107 98-99 -0.8804326258693607 1107 100-101 0.23548216006466305 1108 1 0.012089678001579784 1108 2 0.04431341085658147 1108 3 -0.005180814680457502 1108 4 0.08822773199366907 1108 5 0.08510161171550124 1108 6 -0.16401293878719514 1108 7 -0.15892475242672788 1108 8 -0.29524352956283906 1108 9 0.17819485899482146 1108 10-11 -0.2102519554778226 1108 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2316 5 0.24292521048465687 2316 6 -0.07480579531380727 2316 7 0.317723796734974 2316 8 0.1886951584997405 2316 9 0.36277772501156136 2316 10-11 0.20148272372730958 2316 12-13 0.28529332095949655 2316 14-15 0.4426987807694047 2316 16-17 0.37164747701104517 2316 18-19 0.29596547444024424 2316 20-21 0.13580676091484634 2316 22-23 0.2768005406914611 2316 24-25 0.15390070273037537 2316 26-27 0.11505119726981405 2316 28-29 0.34933549663894325 2316 30-31 0.13860348323039062 2316 32-33 0.21006882702609886 2316 34-35 0.17593554770533615 2316 36-37 0.2525193418892897 2316 38-39 -0.018887690400148927 2316 40-41 -0.6158076823776284 2316 42-43 -0.037577696370263425 2316 44-45 -0.10292507830297382 2316 46-47 0.14925289123337393 2316 48-49 0.06297643456605329 2316 50-51 0.11918449523959396 2316 52-53 0.5853851892157635 2316 54-55 -0.0026413750514748813 2316 56-57 0.02250281914660235 2316 58-59 0.3158208342463027 2316 60-61 0.047790228890512765 2316 62-63 0.25496035995891475 2316 64-65 0.06929725416800636 2316 66-67 0.05823508689642409 2316 68-69 0.012727371547665456 2316 70-71 0.38128387013806275 2316 72-73 0.805258424005487 2316 74-75 0.23811709066847442 2316 76-77 0.29328718399258946 2316 78-79 0.5403309805996415 2316 80-81 0.30671075455958174 2316 82-83 0.0618973449517739 2316 84-85 0.6060532423684606 2316 86-87 0.382255171933803 2316 88-89 0.33057704178728997 2316 90-91 0.3551974179932138 2316 92-93 0.23035509270577492 2316 94-95 0.6931247006955346 2316 96-97 0.9095233909035372 2316 98-99 0.6882350248878382 2316 100-101 0.6463672948303909 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 11.0 6 24.0 7 53.0 8 124.0 9 236.0 10 395.0 11 668.0 12 1062.0 13 1582.0 14 2001.0 15 2750.0 16 3915.0 17 5307.0 18 4486.0 19 2658.0 20 2324.0 21 2255.0 22 2366.0 23 2566.0 24 2736.0 25 3097.0 26 3563.0 27 4143.0 28 4925.0 29 6024.0 30 7282.0 31 9012.0 32 11282.0 33 14677.0 34 19643.0 35 28417.0 36 39449.0 37 64507.0 38 65359.0 39 7756.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.02297301436579 23.312402364688943 16.54026403651178 20.124360584433486 2 14.745561912808691 24.199093249576777 40.44590569428245 20.60943914333208 3 17.38652609279957 28.549780965588177 32.9347549906478 21.128937950964456 4 11.356727616703555 20.320454538497096 40.591928635496735 27.73088920930261 5 10.804827864937355 40.41530416038995 36.272672716806895 12.507195257865794 6 25.9155515963032 41.86388947563376 18.609506491439138 13.611052436623899 7 23.763167320249433 36.625737385240356 23.540306311436016 16.0707889830742 8 24.056743841474802 36.51002109220262 21.8611955513514 17.572039514971177 9 25.03390364934902 17.418057252013554 23.547347249901275 34.00069184873615 10-11 22.236355120445968 29.401887583764207 29.611891226684545 18.74986606910528 12-13 23.513006315647683 28.221730358886752 29.838144307804406 18.42711901766116 14-15 20.594898074762522 29.50872617178053 29.297344953943078 20.59903079951387 16-17 17.823676533164353 32.79638524341749 30.192921713948103 19.187016509470062 18-19 17.518314093203657 30.710889882783682 34.33452417031724 17.43627185369542 20-21 18.61884338809959 29.60607479925672 34.45069965499402 17.324382157649673 22-23 18.59617919019404 29.632628570816312 34.21738604689607 17.553806192093578 24-25 18.343787596315448 29.96378508606782 34.38580057000989 17.306626747606845 26-27 18.03410927703762 30.14715786637931 34.56709810932602 17.251634747257054 28-29 17.740449228376637 29.958303867454056 34.90260187540755 17.39864502876176 30-31 18.316542225732487 30.083327976097546 34.49187383862781 17.108255959542156 32-33 17.674592314356474 30.50853943384732 34.419780751298745 17.397087500497456 34-35 18.060313291148926 30.15710476610309 34.44166888609293 17.340913056655065 36-37 18.316695289612166 30.072613504519975 33.82068872623301 17.79000247963485 38-39 17.71913390334322 30.64981739479154 34.14533721503332 17.485711486831914 40-41 17.862554758602954 30.433844260563703 34.22998154049611 17.47361944033723 42-43 17.842266483579568 30.4077668119423 33.686036240836074 18.063930463642055 44-45 18.246745096598616 30.500273984344627 33.34680295474513 17.906177964311627 46-47 18.597720572703814 30.572673199433055 32.710516406917264 18.119089820945874 48-49 18.366900242147057 30.745176191831895 32.585004025580034 18.302919540441007 50-51 18.436238179641894 30.854310738043417 32.00994302962398 18.69950805269071 52-53 18.49333100676236 31.093855709741902 32.180915383226036 18.231897900269697 54-55 18.91456280364047 30.67844034029162 31.851674978035334 18.555321878032576 56-57 18.926348722375796 31.039364968575985 31.2981959891141 18.73609031993412 58-59 18.68557923963987 30.671093274067 31.82182752149782 18.821499964795308 60-61 18.834510394568067 30.89686249659433 31.60983404814165 18.65879306069595 62-63 18.50741288369288 30.805636424305323 32.05188253265618 18.63506815934562 64-65 18.6367518620221 30.405986634462028 32.023259587156105 18.934001916359776 66-67 18.30644000967364 30.651194969708655 31.50054643805046 19.54181858256725 68-69 18.30322566820037 30.289658085905575 31.845093231209116 19.562023014684947 70-71 18.451697631489523 30.181594986851813 31.934023345302933 19.43268403635573 72-73 18.350981598660386 29.707403087604582 31.837593101104815 20.10402221263022 74-75 18.192560483192054 30.037255748314003 31.68100875219264 20.089175016301304 76-77 17.86178943920456 29.671586139759565 31.992646811220194 20.47397760981568 78-79 17.68913338292603 29.533981711927655 31.621007711358256 21.155877193788054 80-81 18.000465314194226 29.62444246481827 31.323910720900262 21.051181500087246 82-83 17.98748241675991 29.55713959906386 31.26824332908325 21.18713465509298 84-85 17.513523276566694 29.295381422217055 31.1956811231284 21.995414178087852 86-87 17.3960160533397 29.155148609720783 31.0803860883301 22.368449248609416 88-89 17.81648253081941 28.693814076366632 31.274930279402806 22.21477311341115 90-91 17.60969897361714 27.902918417487545 31.83547258769412 22.651910021201196 92-93 17.66922644804695 28.314692389994967 31.300712830489612 22.715368331468476 94-95 17.360811361013408 28.473249025748405 31.0213034307738 23.14463618246439 96-97 17.242186854261757 28.27702113199923 30.831045028332127 23.64974698540689 98-99 17.304943044930372 28.96963518754917 30.482977765940838 23.242444001579617 100-101 17.182199497887453 29.10920947890515 30.0923091053824 23.616281917824995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 814.0 1 554.5 2 382.0 3 464.0 4 368.0 5 393.5 6 765.0 7 1318.0 8 1644.0 9 1605.5 10 1363.5 11 1139.0 12 1065.0 13 1037.5 14 979.5 15 890.0 16 868.0 17 892.0 18 934.5 19 1041.0 20 1224.5 21 1376.5 22 1509.5 23 1741.5 24 2067.5 25 2557.5 26 3280.0 27 4293.0 28 4961.0 29 5659.5 30 6768.0 31 7799.5 32 9190.5 33 10618.0 34 12180.0 35 13870.0 36 15474.5 37 16444.0 38 16061.5 39 15289.0 40 14540.0 41 13550.0 42 12515.5 43 12180.0 44 11951.0 45 11222.0 46 10226.0 47 9515.0 48 9310.5 49 8512.0 50 7212.5 51 5958.5 52 4930.0 53 4203.5 54 3570.0 55 3039.0 56 2577.5 57 2150.5 58 1801.5 59 1473.0 60 1209.0 61 964.5 62 751.0 63 577.5 64 430.0 65 353.0 66 306.5 67 232.0 68 171.5 69 153.0 70 128.0 71 103.5 72 90.5 73 72.0 74 49.0 75 45.0 76 35.0 77 20.0 78 14.5 79 9.0 80 8.0 81 8.5 82 5.5 83 3.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05847040203758637 2 0.0 3 0.0 4 0.0 5 0.017449282283468183 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003673533112309091 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.653193983977272E-4 24-25 0.0 26-27 0.0015306387967954544 28-29 0.003673533112309091 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012398174254043182 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5306387967954548E-4 84-85 6.122555187181819E-4 86-87 0.0 88-89 0.0 90-91 0.008724641141734091 92-93 0.0016837026764750002 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012551238133722727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 326661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.45985316897895 #Duplication Level Percentage of deduplicated Percentage of total 1 87.9936699842275 54.96071707014439 2 6.77652342756815 8.465213165641051 3 1.8495219016053241 3.465625992212379 4 0.8224153490778313 2.0547176778926364 5 0.4703212887586333 1.4688099319054595 6 0.32404383744694926 1.214383830434936 7 0.24467483614867702 1.0697648037993244 8 0.17369324168747963 0.8679083497795161 9 0.1375369596102888 0.7731484472309785 >10 1.052056999724251 12.952796187948898 >50 0.11236327406684368 4.797502297435873 >100 0.03925354552553465 3.7181102512132185 >500 0.0019626772762767327 0.87313683693591 >1k 0.0019626772762767327 3.3181651574254465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3773 1.15502003606185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3035 0.9290977496548409 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2540 0.7775645087720909 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1479 0.4527629560920955 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 906 0.27735174997933637 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.25653506234291823 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.17326831179724544 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 539 0.16500286229455 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.12826753117145911 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.11694080407517274 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.5306387967954546E-4 0.0 22-23 0.0 0.0 0.0 0.0015306387967954547 0.0 24-25 0.0 0.0 0.0 0.0033674053529500004 0.0 26-27 0.0 0.0 0.0 0.005663363548143182 0.0 28-29 0.0 0.0 0.0 0.01117366321660682 0.0 30-31 0.0 0.0 0.0 0.024796348508086364 0.0 32-33 0.0 0.0 0.0 0.042092566911875004 0.0 34-35 0.0 0.0 0.0 0.06352151006701137 0.0 36-37 0.0 0.0 0.0 0.08403206994407045 0.0 38-39 0.0 0.0 0.0 0.11250195156446591 0.0 40-41 0.0 0.0 0.0 0.1484719632891591 0.0 42-43 0.0 0.0 0.0 0.22071811449790454 0.0 44-45 0.0 0.0 0.0 0.2992398847735114 0.0 46-47 0.0 0.0 0.0 0.38128212428174774 0.0 48-49 0.0 0.0 0.0 0.4963861618007659 0.0 50-51 0.0 1.5306387967954546E-4 0.0 0.6571032354642887 0.0 52-53 0.0 3.061277593590909E-4 0.0 0.9304753245719568 0.0 54-55 0.0 3.061277593590909E-4 0.0 1.1752244681795498 0.0 56-57 0.0 3.061277593590909E-4 0.0 1.534465393787443 0.0 58-59 0.0 3.061277593590909E-4 0.0 2.0121777622673047 0.0 60-61 0.0 3.061277593590909E-4 0.0 2.5029005605199273 0.0 62-63 0.0 3.061277593590909E-4 0.0 3.121125570545612 0.0 64-65 0.0 3.061277593590909E-4 0.0 3.576796740351618 0.0 66-67 0.0 3.061277593590909E-4 0.0 4.284411056110157 0.0 68-69 0.0 3.061277593590909E-4 0.0 5.12212966959631 0.0 70-71 0.0 3.061277593590909E-4 0.0 5.909337202788212 0.0 72-73 0.0 3.061277593590909E-4 0.0 6.956906395315021 0.0 74-75 0.0 3.061277593590909E-4 0.0 7.973556684146562 0.0 76-77 0.0 3.061277593590909E-4 0.0 8.814795766865345 0.0 78-79 0.0 3.061277593590909E-4 0.0 9.781241103161992 0.0 80-81 0.0 3.061277593590909E-4 0.0 11.12636647778584 0.0 82-83 0.0 3.061277593590909E-4 0.0 12.30113175432635 0.0 84-85 0.0 3.061277593590909E-4 0.0 13.825647995934624 0.0 86-87 0.0 3.061277593590909E-4 0.0 15.396389529206118 0.0 88-89 0.0 3.061277593590909E-4 0.0 17.40734278043599 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 43.240314 1 CATTCCG 70 4.294041E-6 40.723473 9 GCTTCGT 50 0.0016123343 38.008575 8 GTATCAA 2505 0.0 36.052635 1 GTGCTAC 80 1.070211E-5 35.649414 1 GTTTAAG 75 3.0613306E-4 31.688368 1 CGCAGAA 60 0.003942491 31.673811 9 GTAAGAC 60 0.003942491 31.673811 3 GTGTAGC 110 2.7110345E-6 30.247988 1 GTACCAC 95 3.4779143E-5 30.00677 3 GGACCCC 80 4.4887958E-4 29.685108 6 GGCTTCG 65 0.005831595 29.237364 7 ATCAACG 3135 0.0 28.642824 3 CCCTATA 150 3.3140168E-8 28.506432 2 TCAACGC 3170 0.0 28.32658 4 CAACGCA 3205 0.0 28.165482 5 CAATGGG 135 4.2012107E-7 28.154501 9 TATCAAC 3205 0.0 28.017242 2 AACGCAG 3270 0.0 27.742413 6 ACGCAGC 70 0.008373709 27.148983 7 >>END_MODULE