FastQCFastQC Report
Thu 26 May 2016
SRR937496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430620
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44171.025730342297153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.7119966559843945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.6072639450095212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16310.3787562119734337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.2143421113742975No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.1730063629185825No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6170.14328177975941667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.14003065347638288No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.12075611908411128No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.10844828386976917No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4540.10542938089266639No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGC250.005017313657.005247
GGTATCA19750.037.2905921
TAGGACG801.0745016E-535.6324234
GTATCAA31450.034.9000471
GGACGTG851.6301055E-533.528596
CGTATAG1151.0308759E-733.0542141
TCGTATG650.005837424729.2334582
TCAACGC38300.028.6547974
CAACGCA38650.028.5115935
ATCAACG38550.028.465653
AACGCAG38750.028.3154356
TATCAAC39250.027.9579832
CCTGTAC856.397152E-427.9437473
TCTATAC908.933588E-426.3913153
GTCCTAC1457.8459925E-726.2154121
ATATTCG1109.457167E-525.9114723
CCGACTA1109.464659E-525.9084579
GTAGGAC1309.994876E-625.5792753
CGCAGAG42950.025.325348
ACGCAGA43300.025.2332677
Produced by FastQC (version 0.11.5)