##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937496_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430620 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.859458455250568 33.0 31.0 34.0 30.0 34.0 2 31.95316984812596 33.0 31.0 34.0 30.0 34.0 3 31.975837165017882 33.0 31.0 34.0 30.0 34.0 4 35.62327806418652 37.0 35.0 37.0 33.0 37.0 5 35.64715294226929 37.0 35.0 37.0 35.0 37.0 6 35.68980307463657 37.0 35.0 37.0 35.0 37.0 7 35.580421253076956 37.0 35.0 37.0 33.0 37.0 8 35.59799823510288 37.0 35.0 37.0 33.0 37.0 9 37.23768519808648 39.0 37.0 39.0 34.0 39.0 10-11 37.15249291718917 39.0 37.0 39.0 34.0 39.0 12-13 37.03424132645952 39.0 37.0 39.0 33.0 39.0 14-15 38.210720588918306 40.0 38.0 41.0 33.5 41.0 16-17 38.186438158933626 40.0 38.0 41.0 33.5 41.0 18-19 38.230233152199155 40.0 38.0 41.0 33.5 41.0 20-21 38.1609946124193 40.0 38.0 41.0 34.0 41.0 22-23 38.013579257814314 40.0 38.0 41.0 33.5 41.0 24-25 37.95905206446519 40.0 38.0 41.0 33.0 41.0 26-27 37.94134155403836 40.0 38.0 41.0 33.0 41.0 28-29 37.85113905531559 40.0 37.5 41.0 33.0 41.0 30-31 37.621027820352055 40.0 37.0 41.0 33.0 41.0 32-33 37.34807602991036 39.5 36.0 41.0 33.0 41.0 34-35 37.32155380613998 40.0 36.0 41.0 33.0 41.0 36-37 37.18596210115648 40.0 36.0 41.0 32.5 41.0 38-39 37.05446565417306 40.0 36.0 41.0 32.0 41.0 40-41 36.846678045608655 39.5 35.5 41.0 31.5 41.0 42-43 36.56167851005527 39.0 35.0 41.0 31.0 41.0 44-45 36.278168687009426 39.0 35.0 41.0 30.0 41.0 46-47 35.881040128187266 39.0 35.0 41.0 29.5 41.0 48-49 35.53688518879754 39.0 35.0 41.0 27.5 41.0 50-51 34.5448748316381 38.0 34.0 40.0 25.5 40.5 52-53 34.66830732432307 38.5 35.0 39.5 25.5 40.5 54-55 35.05410919139845 39.0 35.0 40.0 26.0 41.0 56-57 34.87368329385537 39.0 35.0 40.0 24.5 41.0 58-59 34.66006688031211 39.0 35.0 40.0 23.5 41.0 60-61 34.40398495192977 38.0 34.5 40.0 22.5 41.0 62-63 34.126247039152844 38.0 34.0 40.0 22.0 41.0 64-65 33.74590474199991 38.0 34.0 40.0 19.5 41.0 66-67 33.43467674515814 37.5 34.0 40.0 18.0 41.0 68-69 32.988307556546374 37.0 33.0 39.5 12.5 41.0 70-71 32.55813478240676 36.0 33.0 39.0 8.0 41.0 72-73 32.102430216896565 36.0 32.0 39.0 4.5 40.0 74-75 31.62966536621615 35.0 31.5 38.5 2.0 40.0 76-77 31.1513782453207 35.0 31.0 37.5 2.0 39.5 78-79 30.628073475453995 35.0 31.0 37.0 2.0 39.0 80-81 30.129494682086296 35.0 30.0 36.5 2.0 39.0 82-83 29.63973805211091 34.0 30.0 36.0 2.0 37.5 84-85 29.097683572523337 34.0 29.0 35.5 2.0 37.0 86-87 28.64246783707213 34.0 29.0 35.0 2.0 37.0 88-89 28.177333844224606 34.0 28.5 35.0 2.0 36.0 90-91 27.669056244484697 34.0 27.0 35.0 2.0 36.0 92-93 27.16470437973155 34.0 26.0 35.0 2.0 35.0 94-95 26.65427406994566 33.0 25.5 35.0 2.0 35.0 96-97 25.973934095025776 33.0 24.5 35.0 2.0 35.0 98-99 25.13647183131299 32.5 18.5 35.0 2.0 35.0 100-101 22.68872671961358 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10238345832872042 1101 2 -0.14594651304850714 1101 3 -0.04987561880025382 1101 4 -0.2211535959049229 1101 5 0.03591782116335196 1101 6 -0.03872587814265671 1101 7 -0.038782982546720746 1101 8 -0.011341925328864022 1101 9 0.12158794149280538 1101 10-11 -0.11661292632075515 1101 12-13 -0.1354289388681238 1101 14-15 -0.008412427624897134 1101 16-17 -5.882649974680021E-4 1101 18-19 0.12804420070406053 1101 20-21 -0.06878474351305641 1101 22-23 -0.04098809298717043 1101 24-25 -0.06684841316580759 1101 26-27 -0.05720931419912034 1101 28-29 -0.11319295725882483 1101 30-31 -0.021969925383693578 1101 32-33 -0.18533609985492205 1101 34-35 0.13944546589116413 1101 36-37 0.06859607038378357 1101 38-39 0.40046810364382424 1101 40-41 -0.09105703986850244 1101 42-43 0.08694420132693637 1101 44-45 0.3859978230014036 1101 46-47 0.6078933622590128 1101 48-49 0.5767643316364683 1101 50-51 0.5684322852932766 1101 52-53 0.7036470255786398 1101 54-55 0.6410747004078061 1101 56-57 0.7267348424620934 1101 58-59 0.8291733521592093 1101 60-61 0.9183474775540148 1101 62-63 0.8934443318457426 1101 64-65 0.8028992128283576 1101 66-67 0.7345593946032949 1101 68-69 0.8111058850004227 1101 70-71 0.6450845583427025 1101 72-73 0.6206247232090156 1101 74-75 0.8646795939843877 1101 76-77 0.945300619463584 1101 78-79 0.7915099071036451 1101 80-81 0.7616408683958973 1101 82-83 0.7154814719257949 1101 84-85 0.7755504277279286 1101 86-87 0.9003627037660955 1101 88-89 0.6262258471799456 1101 90-91 0.5751667747777631 1101 92-93 0.6357014271193506 1101 94-95 0.9583243292074819 1101 96-97 0.9746488027668114 1101 98-99 1.246637690590191 1101 100-101 1.4365528572651343 1102 1 0.06867300770551665 1102 2 0.22405348695149385 1102 3 0.17728641379802212 1102 4 0.12256644244599357 1102 5 0.06429749518061101 1102 6 0.1307851477442057 1102 7 0.005723249476289993 1102 8 -0.03441958592330252 1102 9 0.00657355990124131 1102 10-11 -0.0661479215268912 1102 12-13 0.024940188648656658 1102 14-15 0.10805928857836022 1102 16-17 0.27133886827194686 1102 18-19 -0.10627027676477496 1102 20-21 -0.04731302730979081 1102 22-23 -0.1320235675797008 1102 24-25 0.050299237840910394 1102 26-27 -0.014932228868339337 1102 28-29 -0.03802996588778029 1102 30-31 -0.16149533286212403 1102 32-33 0.009237246262053134 1102 34-35 0.004675571356166586 1102 36-37 0.18292972330804247 1102 38-39 0.20740003077708735 1102 40-41 0.3109899399972704 1102 42-43 0.44553001148992877 1102 44-45 0.6500821950052398 1102 46-47 0.8294705434670604 1102 48-49 0.8493817813008988 1102 50-51 0.7193431194255879 1102 52-53 0.5056700361251316 1102 54-55 0.5754754674260241 1102 56-57 0.5157952451466556 1102 58-59 0.42822896097992214 1102 60-61 0.6371777747735692 1102 62-63 0.49669936540279025 1102 64-65 0.5856077459060245 1102 66-67 0.3731979372686851 1102 68-69 0.5762161438690683 1102 70-71 0.5063645199917985 1102 72-73 0.48977620930680743 1102 74-75 0.3007246563046131 1102 76-77 0.24076083039359375 1102 78-79 0.4739116328946338 1102 80-81 0.48740309274873184 1102 82-83 0.5631947988673112 1102 84-85 0.43063671727730224 1102 86-87 0.25917861939408837 1102 88-89 0.4119065758472509 1102 90-91 0.36115910459559686 1102 92-93 0.6736256840704549 1102 94-95 0.5552898133877306 1102 96-97 0.7391837979729452 1102 98-99 0.7189387452402407 1102 100-101 1.4882259157502737 1103 1 0.07856311759562828 1103 2 0.05559194848995119 1103 3 0.14025344676505114 1103 4 -0.23149949161994243 1103 5 -0.09768052679741146 1103 6 -0.09317089621183783 1103 7 0.13539357914661565 1103 8 0.02217382067010476 1103 9 0.09668345001113465 1103 10-11 0.09093999056101865 1103 12-13 0.07917095787942685 1103 14-15 0.06783950835858121 1103 16-17 0.14584436277743862 1103 18-19 0.10900444850994973 1103 20-21 -0.030225115221881538 1103 22-23 -0.0218037873599215 1103 24-25 0.08760693014860266 1103 26-27 -0.15169047062658336 1103 28-29 -0.04940359226141311 1103 30-31 -0.1078140141808035 1103 32-33 -0.18416934714453959 1103 34-35 -0.1790057473251494 1103 36-37 -0.21377357339525105 1103 38-39 -0.3401823868053313 1103 40-41 -0.07401006000273469 1103 42-43 -0.0481513071913966 1103 44-45 -0.17343428851124543 1103 46-47 -0.10168330268678716 1103 48-49 -0.03523360331448089 1103 50-51 -0.18505248497001503 1103 52-53 -0.08312117266607544 1103 54-55 -0.23122782927727314 1103 56-57 -0.057281677930269836 1103 58-59 -0.04699081923985915 1103 60-61 0.040474478070272824 1103 62-63 0.1747762884797126 1103 64-65 0.3817066470049255 1103 66-67 0.3938572779280207 1103 68-69 0.4966557043086297 1103 70-71 0.5715843002115761 1103 72-73 0.34972126425185834 1103 74-75 0.45517520575516457 1103 76-77 0.6045520391848029 1103 78-79 0.5859445999276005 1103 80-81 0.6023481476937853 1103 82-83 0.6533596340321459 1103 84-85 0.6611312227718074 1103 86-87 0.8329698281852984 1103 88-89 0.8096538285945059 1103 90-91 0.6128074562439458 1103 92-93 0.6734608489056164 1103 94-95 0.5342458573437732 1103 96-97 0.6609420397311858 1103 98-99 0.9190486353501299 1103 100-101 1.373006135530492 1104 1 0.019837552731448937 1104 2 0.2058686684696447 1104 3 7.61189847054311E-4 1104 4 0.07664470741534757 1104 5 -0.08398161844503704 1104 6 0.03323682148300833 1104 7 -0.10513955108948636 1104 8 0.076136755425118 1104 9 0.06042551652547701 1104 10-11 -0.06350530012189637 1104 12-13 0.0450226968994798 1104 14-15 0.016163956187980943 1104 16-17 0.07009417237378557 1104 18-19 -0.10748196936260968 1104 20-21 -0.2453634752117253 1104 22-23 -0.19402262462825348 1104 24-25 -0.1336446565485332 1104 26-27 -0.018758254328027135 1104 28-29 0.04906810106177062 1104 30-31 -0.045194059877680104 1104 32-33 -0.07429882163045676 1104 34-35 -0.02684022308041989 1104 36-37 -0.057947221529296655 1104 38-39 0.18521238344092694 1104 40-41 0.48711205220848797 1104 42-43 0.21097791342297967 1104 44-45 0.43500440151160547 1104 46-47 0.570048101222838 1104 48-49 0.7147613192546913 1104 50-51 0.5923275607268579 1104 52-53 0.8601867735131634 1104 54-55 0.991925255261954 1104 56-57 1.0805147170938483 1104 58-59 1.158660361262811 1104 60-61 0.9299971995732008 1104 62-63 1.2803792333895885 1104 64-65 1.384473846801825 1104 66-67 1.2202040664530287 1104 68-69 1.2612609340623777 1104 70-71 1.2207185982567594 1104 72-73 1.439929438915481 1104 74-75 1.3332965563517654 1104 76-77 1.3919371623125016 1104 78-79 1.2775820713670534 1104 80-81 1.2426883355587215 1104 82-83 1.1561674532756037 1104 84-85 1.3674424692810732 1104 86-87 0.9947443902568907 1104 88-89 1.1233375046544154 1104 90-91 1.1712816882825372 1104 92-93 1.2827322375829482 1104 94-95 1.1454619020251648 1104 96-97 1.119230945544846 1104 98-99 1.34582700549484 1104 100-101 1.1891853688384408 1105 1 0.04083552408821589 1105 2 0.021025964015713328 1105 3 -0.15394556523212444 1105 4 -0.10246632295374525 1105 5 -0.16558454938033407 1105 6 -0.15650187715749553 1105 7 -0.197972687614147 1105 8 -0.08517974319722299 1105 9 -0.03751556198604078 1105 10-11 -0.19397885206424803 1105 12-13 -0.2135460498834547 1105 14-15 -0.23201934838100868 1105 16-17 -0.2155811841526969 1105 18-19 -0.06355730166648499 1105 20-21 -0.3226668936269661 1105 22-23 -0.32304584805152103 1105 24-25 -0.13827874381046712 1105 26-27 -0.10771728784606438 1105 28-29 -0.16934713495724196 1105 30-31 -0.3999946775541261 1105 32-33 -0.5243669477091188 1105 34-35 -0.38095352431879803 1105 36-37 -0.32735861220965745 1105 38-39 -0.20522120120194387 1105 40-41 -0.1294229040394299 1105 42-43 -0.17780550620339142 1105 44-45 -0.38181819817955187 1105 46-47 -0.3413092730466971 1105 48-49 -0.0975906957633157 1105 50-51 -0.3207617298535723 1105 52-53 -0.4803194789469458 1105 54-55 -0.35398718001827234 1105 56-57 -0.5582414521010506 1105 58-59 -0.6265744466216532 1105 60-61 -0.6205417534046731 1105 62-63 -0.221511643771521 1105 64-65 -0.05248530782923666 1105 66-67 0.17765403163303972 1105 68-69 -0.32903942624888316 1105 70-71 -0.397455925617642 1105 72-73 0.050949210617421414 1105 74-75 -0.25483890857087843 1105 76-77 -0.09274506737835964 1105 78-79 -0.19049858991008506 1105 80-81 -0.18202023621588737 1105 82-83 0.02444643713729633 1105 84-85 -0.01661754222466172 1105 86-87 -0.1590979205797005 1105 88-89 -0.21779853555511153 1105 90-91 -0.02008598059444111 1105 92-93 0.019163036626157037 1105 94-95 0.03427408599585746 1105 96-97 -0.04706784029704281 1105 98-99 -0.052765055546128536 1105 100-101 0.035807829249616674 1106 1 -0.19838898690364815 1106 2 -0.15559011891852847 1106 3 -0.038853148945317884 1106 4 0.13481561884611182 1106 5 -0.09443266354954716 1106 6 -0.2156059873261782 1106 7 -0.37680445342877533 1106 8 -0.23600389260194987 1106 9 -0.5614168563910624 1106 10-11 -0.8244752650429135 1106 12-13 -0.1998711321060611 1106 14-15 -0.23319258323954273 1106 16-17 -0.34398308440849235 1106 18-19 -0.5612358353152445 1106 20-21 -0.537380412754537 1106 22-23 -0.4955306056150377 1106 24-25 -0.3702772820752358 1106 26-27 -0.7609744570803798 1106 28-29 -0.3245334100327284 1106 30-31 -0.5402539432245064 1106 32-33 -0.587085005909266 1106 34-35 -0.0595217931242118 1106 36-37 -0.5816255327566537 1106 38-39 -0.26438493478145375 1106 40-41 -0.07153626545346015 1106 42-43 -0.11118307626089319 1106 44-45 0.16711723543650692 1106 46-47 -0.17408291565243417 1106 48-49 -0.009863501717973122 1106 50-51 0.30122392206111215 1106 52-53 0.6558257713752127 1106 54-55 0.4000816369378484 1106 56-57 0.5157742807860615 1106 58-59 0.6692307579251136 1106 60-61 0.47721371367743615 1106 62-63 0.35538909286610476 1106 64-65 0.035609243360347875 1106 66-67 0.39221560722975113 1106 68-69 0.28318529630992373 1106 70-71 0.572343855122071 1106 72-73 0.35709426860600146 1106 74-75 0.5999953960470528 1106 76-77 0.9612025794202488 1106 78-79 1.05058578683294 1106 80-81 0.36068551263491955 1106 82-83 0.3139450234854557 1106 84-85 0.42724797813384896 1106 86-87 0.44372938309579624 1106 88-89 0.8651126255627339 1106 90-91 0.8316382899804431 1106 92-93 0.662540029682912 1106 94-95 0.9082532755021369 1106 96-97 1.1166830492457827 1106 98-99 0.9196485385915452 1106 100-101 1.848472995714335 1107 1 0.012198709376914252 1107 2 -0.2218847028939841 1107 3 0.016639930038952144 1107 4 -0.04737994670569634 1107 5 0.2705415822194013 1107 6 0.017445507252247694 1107 7 0.03784560835361361 1107 8 0.10055045507322546 1107 9 0.24300370811947403 1107 10-11 0.12530745532583154 1107 12-13 -0.01902228375752202 1107 14-15 0.19488994136928284 1107 16-17 0.04228178848639175 1107 18-19 0.03246513793089889 1107 20-21 0.014870826364123957 1107 22-23 -0.030399474233753665 1107 24-25 -0.07935859880368668 1107 26-27 -0.09711923612157136 1107 28-29 0.03276315930931872 1107 30-31 0.04393670750368983 1107 32-33 0.09054282809743341 1107 34-35 0.25330534114488046 1107 36-37 0.08433306610211133 1107 38-39 0.37254982579443663 1107 40-41 0.35870517178534556 1107 42-43 0.239921370682616 1107 44-45 0.4617567456201854 1107 46-47 0.6818877620101134 1107 48-49 0.30845462898301435 1107 50-51 0.7250510979812503 1107 52-53 0.5996246245830363 1107 54-55 0.7410509956505607 1107 56-57 0.5414243842194963 1107 58-59 0.43319427160748347 1107 60-61 1.0765722875455666 1107 62-63 1.0197788356014712 1107 64-65 1.0048430029227333 1107 66-67 1.112396928123303 1107 68-69 1.0982060524152715 1107 70-71 1.0991254786799018 1107 72-73 1.0245523051756145 1107 74-75 0.81825382691326 1107 76-77 0.7350512750482778 1107 78-79 0.9022733484417813 1107 80-81 1.1661195859685343 1107 82-83 0.9938775487884719 1107 84-85 0.6406540619635237 1107 86-87 0.8513057086403855 1107 88-89 0.9681017824066949 1107 90-91 1.140966736257532 1107 92-93 0.7947751637298701 1107 94-95 0.9876471139238383 1107 96-97 0.9477394586478134 1107 98-99 1.0958324696174095 1107 100-101 1.3967673853182312 1108 1 -0.0266306075980971 1108 2 0.07125250415051099 1108 3 0.11488206139366497 1108 4 0.07304029291984193 1108 5 -0.06522163433852057 1108 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62-63 -1.8168080972837757 2316 64-65 -1.2829316997229796 2316 66-67 -1.6859705062281165 2316 68-69 -1.8929309776661114 2316 70-71 -1.7011941154026644 2316 72-73 -1.6360766691580153 2316 74-75 -1.3451921880450648 2316 76-77 -1.8367338391160004 2316 78-79 -2.1938602221160295 2316 80-81 -1.6534497857160915 2316 82-83 -1.7489480582755483 2316 84-85 -1.3713142422109712 2316 86-87 -1.4827936620558013 2316 88-89 -1.7691062173297212 2316 90-91 -1.448478422056855 2316 92-93 -0.9400523543090777 2316 94-95 -0.9171089072945726 2316 96-97 -1.1214345389140483 2316 98-99 -1.1359226620092286 2316 100-101 -0.8936184339298983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1799.0 3 372.0 4 251.0 5 221.0 6 289.0 7 312.0 8 331.0 9 415.0 10 595.0 11 860.0 12 1382.0 13 2016.0 14 2344.0 15 3148.0 16 4212.0 17 5771.0 18 4823.0 19 2982.0 20 2862.0 21 2935.0 22 3032.0 23 3406.0 24 3786.0 25 4236.0 26 4664.0 27 5411.0 28 6261.0 29 7568.0 30 9254.0 31 11538.0 32 14627.0 33 19526.0 34 26255.0 35 38713.0 36 53050.0 37 83676.0 38 84585.0 39 13112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.14220260378835 22.607839302676428 15.87114227710417 21.37881581643106 2 13.877756210638031 23.066055959745476 41.449870364422594 21.606317465193893 3 16.736367592676633 28.216889732902327 32.36496754659722 22.68177512782382 4 10.644987862693668 19.422250254266675 41.247291507143906 28.68547037589575 5 10.6836472981339 39.86049629246903 36.51208147724046 12.943774932156604 6 25.903559763567074 41.38647098947506 19.163731431192105 13.546237815765766 7 23.87532941603732 35.85908908899588 23.788726754635107 16.4768547403317 8 23.395137167266082 36.01568956085524 22.454229081555454 18.134944190323218 9 24.82728440811158 17.07579933334575 23.53238300962738 34.56453324891529 10-11 21.705776435903314 28.95830011301697 30.271089420750197 19.064834030329518 12-13 23.04003501142993 27.7824076876161 29.994366511939734 19.183190789014233 14-15 20.360844538685523 29.28454883376665 28.941347342254165 21.41325928529366 16-17 17.875416689945432 32.441407341192246 29.94894966199229 19.73422630687003 18-19 17.582431650774474 30.722847396555274 33.642215619388125 18.052505333282124 20-21 18.803255680799907 29.904643092384713 33.31417040421367 17.977930822601706 22-23 18.567826009037493 29.810696734236465 33.12231106621716 18.499166190508888 24-25 18.200479437708008 30.225871017292338 33.26017641445761 18.313473130542043 26-27 17.894179463158324 30.399925519472585 33.419645402864006 18.28624961450508 28-29 17.758283148306358 30.23509172977138 33.7935923012638 18.213032820658462 30-31 18.25461672940251 30.28127538822894 33.4283100430887 18.03579783927985 32-33 17.61429898253479 30.767091017860928 33.35451597660133 18.264094023002954 34-35 17.97949510657516 30.561090986972594 33.20603134399241 18.25338256245984 36-37 18.11871140727155 30.34712217238803 32.69358333701853 18.840583083321892 38-39 17.542959285559675 30.952796572101544 33.06974730653484 18.434496835803944 40-41 17.846137733609517 30.658295901505806 33.18744617962622 18.308120185258453 42-43 17.866261681155375 30.57431135006808 32.6621978610248 18.89722910775175 44-45 18.10041599448755 30.62692487656347 32.398102301587855 18.874556827361125 46-47 18.244833534707332 30.68346102540106 32.04093130236597 19.03077413752564 48-49 18.181013931311927 30.759354279967223 32.00837648066751 19.051255308053342 50-51 18.238126889413234 30.823117745729938 31.654721481428023 19.284033883428805 52-53 18.1611692442383 31.08034479386412 31.748021999003434 19.010463962894146 54-55 18.6072174380679 30.620799314620424 31.551323150418003 19.220660096893674 56-57 18.652764120258578 31.027388094005108 30.94569511762276 19.374152668113556 58-59 18.449393230711948 30.446786973367306 31.57189896098759 19.531920834933157 60-61 18.589217797603048 30.684262759319562 31.411887484598765 19.31463195847862 62-63 18.327071496639086 30.562378481777063 31.86567051297416 19.244879508609685 64-65 18.407118565582973 30.160128167688388 31.884039422966172 19.54871384376247 66-67 18.180210472428577 30.42170111518973 31.48197488059849 19.916113531783196 68-69 18.30314186017018 30.086600989692354 31.76463333329455 19.845623816842924 70-71 18.468732619860422 29.859050704048368 31.891610759339024 19.780605916752187 72-73 18.127035508459688 29.661903426577414 31.82787641562014 20.383184649342763 74-75 18.157647012279483 29.948883001067024 31.52311900963578 20.37035097701771 76-77 17.986339901151066 29.601264298124853 31.976711032700216 20.435684768023872 78-79 17.881916766646146 29.45145302865096 31.605656879732543 21.060973324970355 80-81 18.147025899886664 29.593337568915484 31.428484988119425 20.83115154307843 82-83 18.171874981819283 29.3513003431356 31.424542165884944 21.05228250916017 84-85 18.15448486428119 28.95229828350151 31.286303384592472 21.60691346762483 86-87 18.207386565290197 29.129211946877426 31.407040730953224 21.256360756879157 88-89 18.53020235754099 28.505533123101806 31.423251917102064 21.54101260225514 90-91 18.44990108227627 28.19643896194577 31.821249854532756 21.5324101012452 92-93 18.754014428671166 28.30591110076798 31.493018384919715 21.447056085641144 94-95 18.55454659697561 28.044756182133828 31.53947117331385 21.861226047576714 96-97 18.774648592099187 27.920738157329733 31.213888976166288 22.090724274404796 98-99 19.093611217145995 28.23826659342501 31.026609066448184 21.64151312298081 100-101 19.49460373898758 28.014948503986115 30.864797948536566 21.625649808489744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1346.0 1 864.0 2 451.0 3 534.0 4 435.0 5 477.5 6 941.0 7 1683.0 8 2239.0 9 2209.0 10 1755.0 11 1454.0 12 1383.0 13 1260.5 14 1145.0 15 1022.5 16 976.5 17 1006.5 18 1027.0 19 1138.0 20 1308.0 21 1480.5 22 1695.0 23 2002.5 24 2439.5 25 3021.0 26 4029.5 27 5282.0 28 6021.0 29 7147.5 30 8725.5 31 10282.0 32 12249.0 33 14294.5 34 16365.0 35 18183.5 36 20362.0 37 22081.0 38 21684.5 39 20467.0 40 19282.5 41 17944.0 42 16435.0 43 15805.5 44 15475.0 45 14661.0 46 13461.5 47 12269.0 48 12234.5 49 11373.5 50 9539.5 51 8005.5 52 6725.5 53 5896.5 54 5140.0 55 4386.5 56 3741.0 57 3205.5 58 2664.5 59 2129.5 60 1749.5 61 1411.0 62 1091.0 63 834.5 64 652.5 65 534.0 66 432.0 67 331.5 68 287.5 69 240.0 70 176.0 71 138.0 72 127.0 73 106.0 74 80.0 75 62.0 76 43.5 77 34.0 78 29.0 79 23.5 80 13.5 81 8.5 82 9.5 83 9.0 84 7.5 85 5.5 86 3.0 87 3.0 88 4.0 89 1.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2535878500766337 2 0.221541033858158 3 0.21527100459802143 4 0.22130881055222704 5 0.221541033858158 6 0.24824671404022106 7 0.24894338395801402 8 0.24035112163856764 9 0.23500998560215502 10-11 0.2411639032093261 12-13 0.24290557800380846 14-15 0.22966884956574243 16-17 0.2424411313919465 18-19 0.23373275741953464 20-21 0.22548883005898473 22-23 0.22421160187636432 24-25 0.22061214063443407 26-27 0.2269982815475361 28-29 0.2422089080860155 30-31 0.24174446147415354 32-33 0.23756444196739585 34-35 0.24708559751056616 36-37 0.2259532766708467 38-39 0.22502438344712278 40-41 0.22061214063443407 42-43 0.22641772328270865 44-45 0.24313780130973944 46-47 0.24871116065208304 48-49 0.2517300636291858 50-51 0.2527750685058752 52-53 0.26612790859690677 54-55 0.2501045004876689 56-57 0.22351493195857133 58-59 0.20551762574892016 60-61 0.20098927128326596 62-63 0.2091170869908504 64-65 0.2021503878129209 66-67 0.20261483442478287 68-69 0.20679485393154057 70-71 0.20691096558450606 72-73 0.21027820352050533 74-75 0.2135293298035391 76-77 0.2263016116297432 78-79 0.22026380567553758 80-81 0.21527100459802143 82-83 0.2105104268264363 84-85 0.21085876178533278 86-87 0.21701267939250382 88-89 0.2171287910454693 90-91 0.22525660675305373 92-93 0.21364544145650458 94-95 0.21225210162091868 96-97 0.22188936881705448 98-99 0.23036551948353534 100-101 0.2038920626074033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 430620.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.645384883587816 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28122183634596 53.80484513211356 2 7.3774857745402285 9.095759000894526 3 1.9862270673766482 3.673251961039002 4 0.8683699143091553 2.1412399035566425 5 0.45768325232174756 1.4107030122073183 6 0.31881140109777667 1.1791950915569 7 0.19927357941154233 0.859900754996831 8 0.19523409521951032 0.9628224753764599 9 0.1264870185018073 0.7017606844489264 >10 1.01852732403096 12.699910330155609 >50 0.11793034135558736 5.085883466291513 >100 0.049357427207601154 4.868948947138112 >500 0.0018838712674656931 0.7915555031172392 >1k 0.0015070970139725544 2.724223737107424 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4417 1.025730342297153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3066 0.7119966559843945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2615 0.6072639450095212 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1631 0.3787562119734337 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.2143421113742975 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 745 0.1730063629185825 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 617 0.14328177975941667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.14003065347638288 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.12075611908411128 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 467 0.10844828386976917 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 454 0.10542938089266639 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.4833495889647487E-4 0.0 18-19 0.0 0.0 0.0 4.6444661186196645E-4 0.0 20-21 0.0 0.0 0.0 6.966699177929497E-4 0.0 22-23 0.0 0.0 0.0 9.288932237239329E-4 0.0 24-25 0.0 0.0 0.0 0.0016255631415168826 0.0 26-27 0.0 0.0 0.0 0.0037155728948957316 0.0 28-29 0.0 0.0 0.0 0.009637267196135803 0.0 30-31 0.0 0.0 0.0 0.019622869351168082 0.0 32-33 0.0 0.0 0.0 0.035646277460405926 0.0 34-35 0.0 0.0 0.0 0.05375969532302262 0.0 36-37 0.0 0.0 0.0 0.0773303608750174 0.0 38-39 0.0 0.0 0.0 0.1108866285820445 0.0 40-41 0.0 0.0 0.0 0.1496679206725187 0.0 42-43 0.0 0.0 0.0 0.21887046583995168 0.0 44-45 0.0 0.0 0.0 0.29573638010310715 0.0 46-47 0.0 0.0 0.0 0.39025126561701734 0.0 48-49 0.0 0.0 0.0 0.5160001857786447 0.0 50-51 0.0 0.0 0.0 0.6700803492638521 0.0 52-53 0.0 0.0 0.0 0.9067158980075241 0.0 54-55 0.0 0.0 0.0 1.1332497329431983 0.0 56-57 0.0 0.0 0.0 1.4506989921508522 0.0 58-59 0.0 0.0 0.0 1.8698620593562771 0.0 60-61 0.0 0.0 0.0 2.311783010542938 0.0 62-63 0.0 0.0 0.0 2.817913705819516 0.0 64-65 0.0 0.0 0.0 3.2431145789791467 0.0 66-67 0.0 0.0 0.0 3.8191444893409505 0.0 68-69 0.0 0.0 0.0 4.511634387627142 0.0 70-71 0.0 0.0 0.0 5.218870465839952 0.0 72-73 0.0 0.0 0.0 6.063234406205007 0.0 74-75 0.0 0.0 0.0 6.90248943383958 0.0 76-77 0.0 0.0 0.0 7.634805629092936 0.0 78-79 0.0 0.0 0.0 8.4717384236682 0.0 80-81 0.0 0.0 0.0 9.57921136965306 0.0 82-83 0.0 0.0 0.0 10.578932701685941 0.0 84-85 0.0 0.0 0.0 11.814709024197668 0.0 86-87 0.0 0.0 0.0 13.089614973758767 0.0 88-89 0.0 0.0 0.0 14.630997166875666 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTCGC 25 0.0050173136 57.00524 7 GGTATCA 1975 0.0 37.290592 1 TAGGACG 80 1.0745016E-5 35.632423 4 GTATCAA 3145 0.0 34.900047 1 GGACGTG 85 1.6301055E-5 33.52859 6 CGTATAG 115 1.0308759E-7 33.054214 1 TCGTATG 65 0.0058374247 29.233458 2 TCAACGC 3830 0.0 28.654797 4 CAACGCA 3865 0.0 28.511593 5 ATCAACG 3855 0.0 28.46565 3 AACGCAG 3875 0.0 28.315435 6 TATCAAC 3925 0.0 27.957983 2 CCTGTAC 85 6.397152E-4 27.943747 3 TCTATAC 90 8.933588E-4 26.391315 3 GTCCTAC 145 7.8459925E-7 26.215412 1 ATATTCG 110 9.457167E-5 25.911472 3 CCGACTA 110 9.464659E-5 25.908457 9 GTAGGAC 130 9.994876E-6 25.579275 3 CGCAGAG 4295 0.0 25.32534 8 ACGCAGA 4330 0.0 25.233267 7 >>END_MODULE