FastQCFastQC Report
Thu 26 May 2016
SRR937496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430620
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46021.068691653894385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.8636384747573266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32420.7528679578282477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16690.38758069759881103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.2563745297478055No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.23640332543774095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.15767962472713762No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.14328177975941667No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.1258650318145929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.035.538811
ATACTGC851.6303888E-533.527776
GTGTATC753.0656948E-431.6835121
ACGCGGC300.003949106731.66879736-37
GGTACAC953.4842273E-530.0020183
GGGCGTA804.4842073E-429.6929427
CATTCCG650.005834836529.2361289
GTATCAA32850.028.2113481
TGTGCGG350.00839242527.1415352-53
TAAGACT1257.3730116E-626.601794
GTACATG19150.026.554591
GTACAGG908.910703E-426.402931
ACATGGG18250.025.7688563
TACATGG19800.025.1910882
GTATAGA950.001221514525.01331
AAGGGCG950.001224828425.0016825
CATGGGA9850.024.5955624
GGGATGA1951.5608748E-824.3634417
TACACAG2751.8189894E-1224.1834455
GGATGAC1801.9416984E-723.7543568
Produced by FastQC (version 0.11.5)