##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937496_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430620 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98371650178812 31.0 31.0 34.0 30.0 34.0 2 32.20464911058474 33.0 31.0 34.0 30.0 34.0 3 32.24293112256746 34.0 31.0 34.0 30.0 34.0 4 35.95912869815615 37.0 35.0 37.0 35.0 37.0 5 35.86826436301147 37.0 35.0 37.0 35.0 37.0 6 35.91526868236496 37.0 35.0 37.0 35.0 37.0 7 35.8425409874135 37.0 35.0 37.0 35.0 37.0 8 35.82694951465329 37.0 35.0 37.0 35.0 37.0 9 37.48916678277832 39.0 37.0 39.0 35.0 39.0 10-11 37.40582299939622 39.0 37.0 39.0 34.0 39.0 12-13 37.32054247364266 39.0 37.0 39.0 34.0 39.0 14-15 38.42223886489248 40.0 38.0 41.0 34.0 41.0 16-17 38.415425433096466 40.0 38.0 41.0 34.0 41.0 18-19 38.477581162045425 40.0 38.0 41.0 34.0 41.0 20-21 38.4208025637453 40.0 38.0 41.0 34.0 41.0 22-23 38.25645116343876 40.0 38.0 41.0 34.0 41.0 24-25 38.182524035112166 40.0 37.5 41.0 33.5 41.0 26-27 38.069621708234635 40.0 37.0 41.0 33.0 41.0 28-29 37.92094421996191 40.0 37.0 41.0 33.0 41.0 30-31 37.89067623426688 40.0 37.0 41.0 33.0 41.0 32-33 37.74689052993358 40.0 36.0 41.0 33.0 41.0 34-35 37.58094375551531 40.0 36.0 41.0 33.0 41.0 36-37 37.3662567925317 40.0 36.0 41.0 33.0 41.0 38-39 37.21046282104872 40.0 35.5 41.0 33.0 41.0 40-41 37.01247387487808 39.0 35.0 41.0 32.0 41.0 42-43 36.746049881566115 39.0 35.0 41.0 31.0 41.0 44-45 36.391041985973715 39.0 35.0 41.0 30.5 41.0 46-47 35.929962612047746 39.0 35.0 41.0 29.5 41.0 48-49 35.535112163856766 39.0 35.0 41.0 27.5 41.0 50-51 35.281312990571735 39.0 35.0 40.0 27.0 41.0 52-53 35.055555942594395 39.0 35.0 40.0 26.0 41.0 54-55 34.83482072360782 38.5 35.0 40.0 25.5 41.0 56-57 34.570914030932144 38.0 34.0 40.0 24.0 41.0 58-59 34.30915075937021 38.0 34.0 40.0 23.0 41.0 60-61 34.009494449862984 38.0 34.0 40.0 22.0 41.0 62-63 33.65188449212763 38.0 33.5 40.0 20.5 41.0 64-65 33.277654312386794 37.0 33.0 40.0 18.5 41.0 66-67 32.88540360410571 37.0 32.5 39.5 16.0 41.0 68-69 32.45133180065952 36.0 32.0 39.0 11.5 40.0 70-71 32.05696205471181 36.0 31.5 39.0 8.0 40.0 72-73 31.57571524778227 35.0 31.0 38.5 6.5 40.0 74-75 31.077139937764155 35.0 31.0 37.5 2.0 39.5 76-77 29.759731317635037 33.5 29.5 36.0 2.0 39.0 78-79 30.33973689099438 34.5 30.0 36.0 2.0 39.0 80-81 30.118628953601785 34.0 30.0 36.0 2.0 38.5 82-83 29.73714295666713 34.0 30.0 36.0 2.0 37.5 84-85 29.321618828665645 34.0 29.5 35.0 2.0 37.0 86-87 28.867680321397053 34.0 29.0 35.0 2.0 36.5 88-89 28.36931285123775 34.0 29.0 35.0 2.0 36.0 90-91 27.838189122660353 34.0 27.5 35.0 2.0 36.0 92-93 27.46174469369746 33.0 27.0 35.0 2.0 35.0 94-95 27.027540522966888 33.0 26.0 35.0 2.0 35.0 96-97 26.554730388741813 33.0 25.5 35.0 2.0 35.0 98-99 25.832998931772792 33.0 24.5 34.5 2.0 35.0 100-101 23.49384376015977 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2871359317080433 1101 2 -0.08566859283285311 1101 3 0.026075677271769848 1101 4 -0.09264783336352878 1101 5 -0.05667653843320153 1101 6 -0.22571758771448458 1101 7 -0.07885225207109414 1101 8 -0.25122711654486096 1101 9 -0.28554739968104315 1101 10-11 -0.15889541324433054 1101 12-13 -0.10787294542205217 1101 14-15 -0.2743481325318555 1101 16-17 -0.19651060463195336 1101 18-19 -0.22904607765076435 1101 20-21 -0.220223771360196 1101 22-23 -0.20530249334205308 1101 24-25 -0.2122702875827045 1101 26-27 -0.14686260194781653 1101 28-29 -0.4485789349631446 1101 30-31 -0.1987881191157541 1101 32-33 0.11879730891079987 1101 34-35 0.15145580722430907 1101 36-37 0.11456533015095971 1101 38-39 0.1386998707322391 1101 40-41 0.2778337119163936 1101 42-43 0.5880597767240516 1101 44-45 0.7228321349900071 1101 46-47 1.0192817289367184 1101 48-49 0.9495762179591765 1101 50-51 0.9976202993336543 1101 52-53 1.1415376599337534 1101 54-55 1.0417339145566231 1101 56-57 1.0431352581934377 1101 58-59 1.1399256168139047 1101 60-61 1.1079698727672707 1101 62-63 0.8973764798601422 1101 64-65 1.1380261233080304 1101 66-67 1.111104711971457 1101 68-69 1.0708170922095945 1101 70-71 1.1449230914969917 1101 72-73 1.171410080042417 1101 74-75 1.1622509456941295 1101 76-77 1.308046041332446 1101 78-79 0.9837477125157399 1101 80-81 1.0994385584439428 1101 82-83 1.0821724331795224 1101 84-85 1.0728895346345375 1101 86-87 1.123993945018654 1101 88-89 1.1890048018838613 1101 90-91 1.1316229423211048 1101 92-93 0.980680172442451 1101 94-95 0.7011815846419118 1101 96-97 0.8592351266672118 1101 98-99 1.0655710315700908 1101 100-101 1.5560208999744383 1102 1 -0.019715030461640026 1102 2 -0.07864078842249711 1102 3 -0.13377091908489547 1102 4 -0.019071610928243388 1102 5 -0.10443301014940687 1102 6 -0.11310013805005781 1102 7 -0.08028082349966326 1102 8 -0.18542654128120262 1102 9 -0.28291077456120206 1102 10-11 -0.15100950720405848 1102 12-13 -0.2722353615294324 1102 14-15 -0.08971246618477835 1102 16-17 -0.2516592144114327 1102 18-19 -0.04272297122697921 1102 20-21 -0.22019500817707183 1102 22-23 -0.16722962661147278 1102 24-25 -0.22318591557887402 1102 26-27 -0.1943266479689143 1102 28-29 -0.1432146013102198 1102 30-31 -0.3286586847916908 1102 32-33 -0.18472138715823405 1102 34-35 -0.21441667599716396 1102 36-37 -0.01577503418268833 1102 38-39 -0.08686101133871205 1102 40-41 -0.11013848367325352 1102 42-43 0.001161406637763207 1102 44-45 0.17392034208492646 1102 46-47 -0.0831391723096857 1102 48-49 0.22692041738391566 1102 50-51 0.11964330988016059 1102 52-53 0.3203008430593499 1102 54-55 0.33684417342526984 1102 56-57 0.3659013176373591 1102 58-59 0.6482429706010606 1102 60-61 0.6437225093923828 1102 62-63 0.5671751375782605 1102 64-65 0.5428343687538657 1102 66-67 0.5159848653750956 1102 68-69 0.31281133957297413 1102 70-71 0.7967974922640053 1102 72-73 0.6088405690165359 1102 74-75 0.7411675324630664 1102 76-77 0.7173844881205582 1102 78-79 0.6145578755071099 1102 80-81 0.5096015498149882 1102 82-83 0.7571197006771229 1102 84-85 0.7641071760535212 1102 86-87 0.5039508002439703 1102 88-89 0.8437603148272927 1102 90-91 0.9494848790420995 1102 92-93 0.9398412462679495 1102 94-95 0.8260137994070149 1102 96-97 0.8945467278177404 1102 98-99 1.3370523355010597 1102 100-101 1.4732500466666707 1103 1 -0.004110634857244833 1103 2 -0.0018276016093139447 1103 3 -0.023661028975006104 1103 4 0.035653663797027946 1103 5 -0.14696048267687445 1103 6 -0.18079244574236242 1103 7 -0.11731379053262714 1103 8 -0.11718478303944124 1103 9 -0.1443393459897706 1103 10-11 -0.007547968742521505 1103 12-13 -0.08146613076020515 1103 14-15 -0.02449268596500076 1103 16-17 -0.04539547814769662 1103 18-19 -0.09398670749071414 1103 20-21 -0.09656863455069242 1103 22-23 -0.06096589034773814 1103 24-25 -0.011702399095362637 1103 26-27 -0.05229368093594644 1103 28-29 -0.005357458453076447 1103 30-31 -0.22849384962685804 1103 32-33 -0.07450160693845476 1103 34-35 -0.06018590676639235 1103 36-37 0.045928262520604335 1103 38-39 0.20973239525469722 1103 40-41 0.1801362416014669 1103 42-43 0.26297459345094865 1103 44-45 0.160348913513495 1103 46-47 0.4001025859320677 1103 48-49 0.3167006371641321 1103 50-51 0.49931363955048624 1103 52-53 0.5028283155868181 1103 54-55 0.2649760415571407 1103 56-57 0.29386835060439154 1103 58-59 0.40686934422743803 1103 60-61 0.6013049269748052 1103 62-63 0.5871751375782637 1103 64-65 0.37750469842418966 1103 66-67 0.631259590649826 1103 68-69 0.65347068023231 1103 70-71 0.9169623274288483 1103 72-73 0.6741152942912585 1103 74-75 0.8876510489465836 1103 76-77 1.2116702024062675 1103 78-79 0.8497776557268928 1103 80-81 0.7755356157490532 1103 82-83 0.8523944259518501 1103 84-85 0.6160851980315449 1103 86-87 0.5885112398044043 1103 88-89 0.565628446695424 1103 90-91 0.695309054866275 1103 92-93 0.8394566308833369 1103 94-95 0.8767830301762451 1103 96-97 1.21575551902653 1103 98-99 1.2345798080285313 1103 100-101 1.590832464249086 1104 1 -0.12633454955640744 1104 2 -0.12975347111934354 1104 3 -0.09193216378079683 1104 4 -0.1569688292215048 1104 5 -0.17045375508104144 1104 6 -0.05026185422167373 1104 7 -0.08842320916679824 1104 8 -0.004384579942211531 1104 9 0.02206329427425402 1104 10-11 -0.19003590984432606 1104 12-13 -0.15761489948322804 1104 14-15 -0.13001185326634612 1104 16-17 -0.3386983468961091 1104 18-19 -0.014644234781911791 1104 20-21 -0.25713984551794766 1104 22-23 -0.46212354457469473 1104 24-25 -0.4421415968612834 1104 26-27 -0.4335604999255338 1104 28-29 -0.3630472274299805 1104 30-31 -0.3851249742777796 1104 32-33 -0.10143991615399273 1104 34-35 -0.15284901923148908 1104 36-37 -0.20527762729914656 1104 38-39 -0.47765780530382784 1104 40-41 -0.11872641389484784 1104 42-43 -0.2294397249039548 1104 44-45 0.10925980460260831 1104 46-47 0.04494899364976135 1104 48-49 0.15915049753478883 1104 50-51 0.17990262152561343 1104 52-53 0.5979057464068305 1104 54-55 0.4519549702192407 1104 56-57 0.44757741381840077 1104 58-59 0.08491199464632615 1104 60-61 0.18119068478135603 1104 62-63 0.14902332239674365 1104 64-65 0.2633412051517823 1104 66-67 0.32952564802479145 1104 68-69 0.6325897459850438 1104 70-71 0.4044896186195075 1104 72-73 0.3951536288939508 1104 74-75 0.6636474947450104 1104 76-77 0.6630563410201304 1104 78-79 0.5572287854552478 1104 80-81 0.4108745342562905 1104 82-83 0.3019146087393594 1104 84-85 0.3053235834085406 1104 86-87 0.04760237968762482 1104 88-89 0.17152080516204293 1104 90-91 0.1526154778162656 1104 92-93 0.649725734716796 1104 94-95 0.682284426469721 1104 96-97 0.631175676426885 1104 98-99 0.6359278659112455 1104 100-101 0.8038535355869918 1105 1 -0.06312263203437851 1105 2 -0.12913882249851838 1105 3 -0.006483894183190841 1105 4 -0.08976623740268508 1105 5 -0.19249329848491925 1105 6 -0.3642927986005162 1105 7 -0.22564124289677778 1105 8 -0.17000059108460874 1105 9 -0.04378888727417518 1105 10-11 -0.2516975805985666 1105 12-13 -0.2374647193275976 1105 14-15 -0.21628520537220197 1105 16-17 -0.2775831986840416 1105 18-19 -0.24198247319290545 1105 20-21 -0.38458557174195107 1105 22-23 -0.4921444365721541 1105 24-25 -0.46815970325908296 1105 26-27 -0.14635154967271546 1105 28-29 -0.206641862122801 1105 30-31 -0.1136455786317967 1105 32-33 -0.018312474969505388 1105 34-35 -0.3225359420522125 1105 36-37 -0.14874357939894622 1105 38-39 -0.31771291173189553 1105 40-41 -0.3417833067400906 1105 42-43 -0.11910726964008234 1105 44-45 0.16319295021074964 1105 46-47 0.09251613568507366 1105 48-49 0.5595940740025256 1105 50-51 0.2732868223310092 1105 52-53 0.18891159011046454 1105 54-55 0.3263592455091455 1105 56-57 0.19113722851546555 1105 58-59 0.36426525107288654 1105 60-61 0.5567107138484815 1105 62-63 0.6424044953764252 1105 64-65 0.6723035692781067 1105 66-67 0.5133767395559587 1105 68-69 0.740203213753837 1105 70-71 0.7541959195248253 1105 72-73 0.6938471220964821 1105 74-75 0.6779827356085413 1105 76-77 0.7067160739659073 1105 78-79 0.7964255032921699 1105 80-81 0.5836513532225887 1105 82-83 0.6825849693533996 1105 84-85 0.6194938077704286 1105 86-87 0.8595274712793533 1105 88-89 0.9365388207250653 1105 90-91 0.9409265566305685 1105 92-93 0.8598871178275864 1105 94-95 0.7113349003244451 1105 96-97 0.4382819833878564 1105 98-99 0.4238740917264856 1105 100-101 0.9620180676365244 1106 1 -0.283494605184611 1106 2 -0.02643653565220916 1106 3 -0.05974875676084679 1106 4 -0.09209347376143029 1106 5 -0.036229955342577114 1106 6 -1.860919284624174E-4 1106 7 -0.22686063781532795 1106 8 -0.23202432504879766 1106 9 -0.10601649483673015 1106 10-11 -0.062277851019580055 1106 12-13 -0.011082321697152508 1106 14-15 0.10427525468973187 1106 16-17 0.3974153588140794 1106 18-19 0.15980622913240694 1106 20-21 0.11119462943898384 1106 22-23 0.048784149968348345 1106 24-25 0.023775749590335238 1106 26-27 0.10299740114759004 1106 28-29 0.1709108853624386 1106 30-31 0.08242696959585061 1106 32-33 0.06011539032005686 1106 34-35 0.18158961331101153 1106 36-37 0.1221659661168033 1106 38-39 0.14511862328243552 1106 40-41 0.09974920725217373 1106 42-43 0.05448725269945953 1106 44-45 -0.037911044557780826 1106 46-47 0.19174103134410103 1106 48-49 0.2783100549999702 1106 50-51 0.408289611167973 1106 52-53 0.5859342662399385 1106 54-55 0.7718846046921115 1106 56-57 0.6950762203028233 1106 58-59 0.7417814551772821 1106 60-61 0.6866185860620462 1106 62-63 1.067594424790002 1106 64-65 1.0961452402722855 1106 66-67 1.0601388036799833 1106 68-69 0.9251204141461997 1106 70-71 0.7511955156242962 1106 72-73 1.0162020544912274 1106 74-75 1.1352885267727402 1106 76-77 1.3577498669765014 1106 78-79 1.459614778771563 1106 80-81 1.252458692672132 1106 82-83 1.1175839115186932 1106 84-85 1.1556255947417533 1106 86-87 1.0064260922475583 1106 88-89 1.3965815187805717 1106 90-91 1.575363884732429 1106 92-93 1.5050508898738215 1106 94-95 1.7930997532854214 1106 96-97 1.6223125259608935 1106 98-99 1.541899595159638 1106 100-101 2.1186199178855993 1107 1 0.05703047568783859 1107 2 -0.11787446864956053 1107 3 -0.13274915938763598 1107 4 0.07364992801846171 1107 5 0.026746428513476417 1107 6 0.05806983987488934 1107 7 -0.15920545295409028 1107 8 -0.28303297458300136 1107 9 -0.14230844091250816 1107 10-11 -0.2869366595219489 1107 12-13 -0.07084463305261579 1107 14-15 0.0545716713112796 1107 16-17 0.21006894705183043 1107 18-19 -0.11011338434587259 1107 20-21 -0.16724956509918343 1107 22-23 -0.011748705766315481 1107 24-25 0.02684404342459601 1107 26-27 -0.04797376244384566 1107 28-29 0.00815247532175789 1107 30-31 -0.19550352868951393 1107 32-33 -0.21219221654958886 1107 34-35 -0.4624188835026857 1107 36-37 -0.3457766109723437 1107 38-39 -0.7022441712251819 1107 40-41 -0.6016127820018582 1107 42-43 -0.716626357474496 1107 44-45 -0.43508155006266236 1107 46-47 -0.4661650316600472 1107 48-49 -0.39748513291567633 1107 50-51 -0.16975751005045936 1107 52-53 -0.09705961105607486 1107 54-55 -0.0952277281830547 1107 56-57 -0.24897253919014162 1107 58-59 -0.4257321161223686 1107 60-61 -0.21484891917904037 1107 62-63 0.10757248857164114 1107 64-65 0.30156820665988704 1107 66-67 -0.028237777324363833 1107 68-69 0.046491566630677994 1107 70-71 0.019886423200624392 1107 72-73 0.2792978380168485 1107 74-75 0.44893479830980354 1107 76-77 0.3276083922517472 1107 78-79 0.09734090925040917 1107 80-81 0.425747875895091 1107 82-83 0.02265423237059494 1107 84-85 0.25998229431274567 1107 86-87 0.32753484313264636 1107 88-89 -0.03500663566151374 1107 90-91 0.13368229310706425 1107 92-93 0.3048412462679515 1107 94-95 0.3474266029390236 1107 96-97 0.2175962390129449 1107 98-99 0.6061046849176464 1107 100-101 1.3560031214064985 1108 1 -0.03942721017381956 1108 2 0.048144048362338765 1108 3 0.11072188540791217 1108 4 0.056526844670209186 1108 5 -0.021796997513391148 1108 6 0.003017798067880051 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56-57 0.11412093384204525 2316 58-59 0.3914732477865641 2316 60-61 0.4194794390299208 2316 62-63 -0.0716308325709889 2316 64-65 -0.45161126024400744 2316 66-67 0.30442836217680025 2316 68-69 -0.08590934272979922 2316 70-71 -0.22892745443108709 2316 72-73 -0.39749205358474526 2316 74-75 0.18971763907287098 2316 76-77 -0.4334044244594004 2316 78-79 -1.0292694166037641 2316 80-81 -0.493809964044285 2316 82-83 -0.8465263547599768 2316 84-85 -0.34489069175031517 2316 86-87 -0.5604095409074183 2316 88-89 -0.4733612203539437 2316 90-91 -0.26637866040352876 2316 92-93 0.021259156715711214 2316 94-95 0.03944663522791103 2316 96-97 0.32942945702882653 2316 98-99 0.8017325060767497 2316 100-101 0.0322905583937505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 2.0 5 15.0 6 35.0 7 68.0 8 155.0 9 283.0 10 536.0 11 860.0 12 1371.0 13 1821.0 14 2461.0 15 3418.0 16 4634.0 17 6422.0 18 5547.0 19 3387.0 20 2861.0 21 2763.0 22 3012.0 23 3334.0 24 3738.0 25 4106.0 26 4722.0 27 5533.0 28 6916.0 29 8426.0 30 10243.0 31 12443.0 32 15557.0 33 20001.0 34 27202.0 35 39055.0 36 53796.0 37 85381.0 38 82003.0 39 8508.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.64713181320712 23.36096142987599 16.423032863260673 20.568873893656225 2 14.68835632344062 24.091774650503925 40.26473456876133 20.955134457294132 3 17.42719799359064 28.206074961683154 32.76392178719056 21.602805257535646 4 11.403325437740932 20.29956806465097 40.39083182388185 27.906274673726255 5 10.785529499737583 40.266224507331394 36.49695551003915 12.45129048289187 6 26.42422553527472 41.67990339510473 18.393479169569456 13.502391900051089 7 23.883238121777904 36.35479076680135 23.520040871301845 16.241930240118897 8 23.912033811713343 36.64948214202777 21.895871069620547 17.542612976638335 9 25.06177139937764 17.242116018763642 23.292694254795414 34.40341832706331 10-11 22.187337080647765 29.290940145534517 29.729958815148873 18.79176395866885 12-13 23.50696702275894 27.912447747329306 29.83000464468184 18.750580585229912 14-15 20.64592912544703 29.454275231062187 29.059379499326553 20.840416144164227 16-17 18.081371046398218 32.4643537225396 30.18299196507361 19.271283265988576 18-19 17.838465468394407 30.595420556407042 33.8573452231666 17.708768752031954 20-21 18.828317309925225 29.59871812735126 34.03580883377455 17.537155728948957 22-23 18.734956196624612 29.54176270123718 33.81829581937566 17.904985282762546 24-25 18.53038779240021 29.905809570918006 33.963591776451004 17.600210860230785 26-27 18.267909994577565 30.005561767550624 34.15726449281673 17.56926374505508 28-29 17.92373520976301 29.919183474413906 34.42842047816445 17.728660837658644 30-31 18.5004824455986 29.99569224266956 34.0046932493611 17.499132062370755 32-33 17.958060470948865 30.28203520505318 34.0874785193442 17.672425804653756 34-35 18.29687427430217 30.034485160930753 33.992731410524364 17.67590915424272 36-37 18.575426129766385 29.92893966838512 33.45327667084669 18.042357531001812 38-39 17.97277401316248 30.435908392124805 33.79619523568453 17.795122359028188 40-41 18.155819696971456 30.297861569204365 33.86458346637809 17.681735267446086 42-43 18.174840035766955 30.1786025338799 33.30867580969192 18.337881620661225 44-45 18.518036867943284 30.236659870233012 33.05214830778091 18.193154954042797 46-47 18.78280154196275 30.33196321582834 32.53541405415448 18.34982118805443 48-49 18.649273141052436 30.414518601086804 32.403859551344574 18.532348706516185 50-51 18.687473874878084 30.551762574892017 31.886930472342208 18.873833077887696 52-53 18.53246481816915 30.82845664390878 32.06272351493196 18.576355022990107 54-55 19.048813338906694 30.405229668849564 31.71775579397148 18.82820119827226 56-57 19.21833635223631 30.7967581626492 31.06033161488087 18.924573870233615 58-59 18.88869536946728 30.32453207003855 31.68919232734197 19.0975802331522 60-61 18.956852909758023 30.733361200130044 31.404834889229484 18.90495100088245 62-63 18.918419952626444 30.5336491570294 31.684083414611493 18.863847475732666 64-65 18.85931912126701 30.175444707630856 31.785681110956293 19.179555060145837 66-67 18.628721378477543 30.511007384701127 31.296154382053782 19.564116854767544 68-69 18.553016580744046 30.24464725279829 31.548465003947797 19.65387116250987 70-71 18.752264177232828 29.987576053132692 31.74922205192513 19.510937717709346 72-73 18.622799684176304 29.527077237471556 31.756304862756025 20.09381821559612 74-75 18.466977845896615 30.0588686080535 31.30811388230923 20.166039663740655 76-77 18.153824717848682 29.758371650178812 31.746551483906927 20.341252148065582 78-79 18.00810459337699 29.508267149691143 31.465561283730437 21.018066973201428 80-81 18.370489062282292 29.7493149412475 31.02480144907343 20.855394547396777 82-83 18.427469357992464 29.60640289072914 30.981984032251326 20.984143719027074 84-85 17.970177813798276 29.416491144143663 30.862200692491506 21.751130349566555 86-87 17.874576192466677 29.461125818587153 30.878384654683945 21.785913334262226 88-89 18.284566439087826 28.924806093539544 31.079373925967207 21.711253541405416 90-91 18.022149271271093 28.39238373490548 31.432363668878033 22.153103324945395 92-93 18.068531484771427 28.724236829576988 31.102983012273427 22.104248673378155 94-95 17.70807208211416 28.651130927499885 30.991477404672334 22.649319585713624 96-97 17.770540151409595 28.533393711392876 30.704449398541637 22.991616738655893 98-99 17.8153799351864 28.77888716140351 30.543438000369232 22.86229490304085 100-101 17.749228925512348 28.88521673696141 30.396244031140263 22.969310306385985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1081.0 1 742.5 2 480.5 3 573.5 4 461.5 5 453.0 6 916.0 7 1748.0 8 2161.0 9 2085.0 10 1748.5 11 1340.5 12 1244.0 13 1228.0 14 1165.0 15 1071.5 16 1049.0 17 1062.5 18 1125.5 19 1222.5 20 1366.5 21 1543.0 22 1750.0 23 2075.5 24 2522.5 25 3174.5 26 4152.0 27 5291.0 28 6123.5 29 7179.5 30 8651.5 31 10123.0 32 12126.0 33 14151.0 34 16051.0 35 18123.0 36 20294.5 37 21896.0 38 21550.5 39 20323.0 40 19285.5 41 17979.5 42 16510.0 43 15923.5 44 15687.5 45 14901.5 46 13623.0 47 12582.5 48 12454.5 49 11505.5 50 9753.5 51 8032.5 52 6682.5 53 5831.0 54 5016.5 55 4276.5 56 3644.5 57 3145.0 58 2573.0 59 2044.5 60 1688.0 61 1367.5 62 1091.5 63 851.5 64 649.0 65 518.5 66 428.0 67 354.0 68 280.0 69 231.0 70 182.0 71 135.5 72 109.5 73 102.0 74 77.0 75 52.0 76 45.5 77 31.5 78 19.0 79 14.0 80 14.0 81 12.5 82 8.5 83 6.0 84 3.5 85 2.0 86 2.0 87 1.5 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06061028284798663 2 0.0 3 0.0 4 0.0 5 0.0013933398355858995 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1611165296549163E-4 12-13 0.004644466118619664 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.80558264827458E-4 24-25 6.966699177929497E-4 26-27 3.4833495889647487E-4 28-29 0.0034833495889647482 30-31 3.4833495889647487E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.644466118619665E-4 40-41 1.1611165296549163E-4 42-43 0.012772281826204079 44-45 4.644466118619665E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.644466118619665E-4 84-85 2.3222330593098325E-4 86-87 0.0 88-89 0.0 90-91 0.009637267196135805 92-93 0.0034833495889647482 94-95 0.0 96-97 0.0 98-99 1.1611165296549163E-4 100-101 0.01207561190841113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 430620.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.768743126809575 #Duplication Level Percentage of deduplicated Percentage of total 1 86.84006682063846 52.77161713738357 2 7.618299505338896 9.259089714060798 3 2.1022685193953414 3.8325664691614145 4 0.8707966956377067 2.1166888285152954 5 0.4750339196356892 1.443360711943135 6 0.3173773065126383 1.1571972008247133 7 0.22516670248776033 0.9578168252932624 8 0.17548440008622168 0.8531173145281509 9 0.15128289713583734 0.8273944363974531 >10 1.050282934198077 12.76585147832932 >50 0.11850877510020945 5.07250245264772 >100 0.05199094649181334 4.94891320095558 >500 0.0011468591137900003 0.4269869250038191 >1k 0.0022937182275800006 3.566897304955733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4602 1.068691653894385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.8636384747573266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3242 0.7528679578282477 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1669 0.38758069759881103 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1104 0.2563745297478055 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1018 0.23640332543774095 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.15767962472713762 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 617 0.14328177975941667 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 542 0.1258650318145929 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1611165296549161E-4 0.0 18-19 0.0 0.0 0.0 2.3222330593098323E-4 0.0 20-21 0.0 0.0 0.0 4.6444661186196645E-4 0.0 22-23 0.0 0.0 0.0 6.966699177929497E-4 0.0 24-25 0.0 0.0 0.0 0.0013933398355858995 0.0 26-27 0.0 0.0 0.0 0.0034833495889647482 0.0 28-29 0.0 0.0 0.0 0.009405043890204821 0.0 30-31 0.0 0.0 0.0 0.019738981004133575 0.0 32-33 0.0 0.0 0.0 0.036575170684129854 0.0 34-35 0.0 0.0 0.0 0.05468858854674655 0.0 36-37 0.0 0.0 0.0 0.0788398123635688 0.0 38-39 0.0 0.0 0.0 0.1121638567646649 0.0 40-41 0.0 0.0 0.0 0.15152570711996655 0.0 42-43 0.0 0.0 0.0 0.22200548047001997 0.0 44-45 0.0 0.0 0.0 0.298058613162417 0.0 46-47 0.0 0.0 0.0 0.3925734986763272 0.0 48-49 0.0 0.0 0.0 0.518902977102782 0.0 50-51 0.0 0.0 0.0 0.6730992522409549 0.0 52-53 0.0 0.0 0.0 0.9099670242905578 0.0 54-55 0.0 0.0 0.0 1.135804189308439 0.0 56-57 0.0 0.0 0.0 1.4553434582694718 0.0 58-59 0.0 0.0 0.0 1.8760159769634481 0.0 60-61 0.0 0.0 0.0 2.3182852631090056 0.0 62-63 0.0 0.0 0.0 2.82476429334448 0.0 64-65 2.3222330593098323E-4 0.0 0.0 3.2537968510519715 0.0 66-67 2.3222330593098323E-4 0.0 0.0 3.8335423343086714 0.0 68-69 2.3222330593098323E-4 0.0 0.0 4.526845014165621 0.0 70-71 2.3222330593098323E-4 0.0 0.0 5.235706655519948 0.0 72-73 2.3222330593098323E-4 0.0 0.0 6.084366727044726 0.0 74-75 2.3222330593098323E-4 0.0 0.0 6.928962890715713 0.0 76-77 2.3222330593098323E-4 0.0 0.0 7.667200780270308 0.0 78-79 2.3222330593098323E-4 0.0 0.0 8.514931958571363 0.0 80-81 2.3222330593098323E-4 0.0 0.0 9.63633830291208 0.0 82-83 2.3222330593098323E-4 0.0 0.0 10.64360689238772 0.0 84-85 2.3222330593098323E-4 0.0 0.0 11.89192327341972 0.0 86-87 2.3222330593098323E-4 0.0 0.0 13.173679810505782 0.0 88-89 2.3222330593098323E-4 0.0 0.0 14.732827086526402 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2260 0.0 35.53881 1 ATACTGC 85 1.6303888E-5 33.52777 6 GTGTATC 75 3.0656948E-4 31.683512 1 ACGCGGC 30 0.0039491067 31.668797 36-37 GGTACAC 95 3.4842273E-5 30.002018 3 GGGCGTA 80 4.4842073E-4 29.692942 7 CATTCCG 65 0.0058348365 29.236128 9 GTATCAA 3285 0.0 28.211348 1 TGTGCGG 35 0.008392425 27.14153 52-53 TAAGACT 125 7.3730116E-6 26.60179 4 GTACATG 1915 0.0 26.55459 1 GTACAGG 90 8.910703E-4 26.40293 1 ACATGGG 1825 0.0 25.768856 3 TACATGG 1980 0.0 25.191088 2 GTATAGA 95 0.0012215145 25.0133 1 AAGGGCG 95 0.0012248284 25.001682 5 CATGGGA 985 0.0 24.595562 4 GGGATGA 195 1.5608748E-8 24.363441 7 TACACAG 275 1.8189894E-12 24.183445 5 GGATGAC 180 1.9416984E-7 23.754356 8 >>END_MODULE