FastQCFastQC Report
Thu 26 May 2016
SRR937495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448301
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101430.4142872955572047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74650.30490532005664334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48580.19842331478033137No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40930.16717715673032035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77200.035.9585151
GTATCAA122600.034.972141
ATCAACG150700.028.0926483
TCAACGC152650.027.7343524
TATCAAC153400.027.6607062
CAACGCA153750.027.476375
AACGCAG158700.026.6090016
ACGCAGA176800.023.5101937
CGCAGAG178100.023.285248
GTACATG103250.022.6967181
GTACCGG2103.4240657E-822.6353321
TACATGG103500.022.1739942
GCAGAGT183100.021.8965459
GAGTACT89750.021.5144312-13
AGAGTAC145750.021.44563110-11
AGTACTT92650.020.99482312-13
GTACTTT92650.020.71135714-15
TGCGCCG1150.003715572620.6606925
ACATGGG108000.020.5897143
CAGAGTA179750.018.01019710-11
Produced by FastQC (version 0.11.5)