##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2448301 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.558940261021828 31.0 31.0 34.0 30.0 34.0 2 31.674691142959954 33.0 31.0 34.0 30.0 34.0 3 31.70137944639977 33.0 31.0 34.0 30.0 34.0 4 35.33303339744582 37.0 35.0 37.0 33.0 37.0 5 35.33466269057604 37.0 35.0 37.0 33.0 37.0 6 35.361043025346966 37.0 35.0 37.0 33.0 37.0 7 35.29299379447217 37.0 35.0 37.0 33.0 37.0 8 35.27064768588503 37.0 35.0 37.0 33.0 37.0 9 36.84886417152139 39.0 37.0 39.0 33.0 39.0 10-11 36.72138107201688 39.0 37.0 39.0 32.5 39.0 12-13 36.57396088144391 39.0 37.0 39.0 32.0 39.0 14-15 37.63679874329178 40.0 37.0 41.0 32.0 41.0 16-17 37.553457887735206 40.0 37.0 41.0 32.0 41.0 18-19 37.48306764568572 40.0 37.0 41.0 32.0 41.0 20-21 37.35258430233864 40.0 37.0 41.0 32.0 41.0 22-23 37.4218639783262 40.0 37.0 41.0 32.0 41.0 24-25 37.38292064578661 40.0 37.0 41.0 32.0 41.0 26-27 37.296309154797555 40.0 37.0 41.0 31.5 41.0 28-29 37.149063575107796 39.5 37.0 41.0 31.0 41.0 30-31 36.8887497901606 39.0 36.0 41.0 30.5 41.0 32-33 36.63663638580387 39.0 36.0 40.5 30.0 41.0 34-35 36.63198172937069 39.0 36.0 41.0 30.0 41.0 36-37 36.48699710533958 39.0 35.5 41.0 30.0 41.0 38-39 36.33231371469439 39.0 35.0 41.0 30.0 41.0 40-41 36.09496360945815 39.0 35.0 40.5 29.5 41.0 42-43 35.81670636086004 39.0 35.0 40.0 28.5 41.0 44-45 35.55397355145466 39.0 35.0 40.0 27.0 41.0 46-47 35.138685153500326 38.0 34.5 40.0 25.5 41.0 48-49 34.783120825421385 38.0 34.0 40.0 24.0 41.0 50-51 33.8132658116792 37.5 33.0 39.5 23.5 40.5 52-53 33.924270340942556 38.0 33.5 39.5 23.0 40.5 54-55 34.21782472825032 38.0 34.0 40.0 23.0 41.0 56-57 33.92859538104179 38.0 33.0 40.0 22.0 41.0 58-59 33.64014800467753 38.0 33.0 40.0 20.5 41.0 60-61 33.28633836280751 37.5 32.5 40.0 18.5 41.0 62-63 32.88408900702977 37.0 32.0 40.0 17.0 41.0 64-65 32.432798091411144 36.0 31.0 39.0 13.0 41.0 66-67 31.9726150910366 36.0 31.0 39.0 8.5 40.0 68-69 31.445787098890207 35.0 30.5 39.0 7.5 40.0 70-71 30.950827328829256 35.0 30.0 38.0 4.5 40.0 72-73 30.432321842779952 35.0 29.0 37.5 2.0 39.5 74-75 29.84812263688166 34.0 29.0 37.0 2.0 39.0 76-77 29.293441451847627 34.0 28.5 36.0 2.0 39.0 78-79 28.70574798605237 34.0 27.5 36.0 2.0 38.0 80-81 28.078479729412358 33.5 26.0 35.0 2.0 37.0 82-83 27.474729005951474 33.0 26.0 35.0 2.0 37.0 84-85 26.838998758731055 32.5 25.0 35.0 2.0 36.0 86-87 26.19732336832767 32.0 23.5 35.0 2.0 36.0 88-89 25.576758331594032 32.0 21.0 34.5 2.0 35.0 90-91 24.94317487923258 31.0 19.0 34.0 2.0 35.0 92-93 24.210467789703962 31.0 12.0 34.0 2.0 35.0 94-95 23.356798244987033 31.0 2.0 34.0 2.0 35.0 96-97 22.429902818321768 30.0 2.0 34.0 2.0 35.0 98-99 21.195387740314608 29.5 2.0 34.0 2.0 35.0 100-101 18.197107912793403 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.014699257340240024 1101 2 0.05114014327408967 1101 3 -0.0334619219507033 1101 4 -0.15468988579900866 1101 5 -0.10685455340913563 1101 6 -0.09964670084948324 1101 7 -0.029527313106285646 1101 8 -0.032081470901161424 1101 9 -0.02393342358114836 1101 10-11 0.014593899479478978 1101 12-13 0.17963375356158906 1101 14-15 0.200779186467976 1101 16-17 0.12566493469827833 1101 18-19 0.13647487703408245 1101 20-21 0.15349550300443582 1101 22-23 0.1861767902306255 1101 24-25 0.2101372283981462 1101 26-27 0.20234601534910723 1101 28-29 0.207100191123061 1101 30-31 0.19381331325693196 1101 32-33 0.015177038601343895 1101 34-35 0.09744270055082893 1101 36-37 0.06318958196879976 1101 38-39 0.138790572351148 1101 40-41 0.25197704722304337 1101 42-43 0.21543673810278108 1101 44-45 0.2951821384619322 1101 46-47 0.5156580923710123 1101 48-49 0.4230897177354933 1101 50-51 0.23976962698846904 1101 52-53 0.23489090688576653 1101 54-55 0.3062272677713693 1101 56-57 0.31565265739175885 1101 58-59 0.3287410842313818 1101 60-61 0.32057029003981086 1101 62-63 0.351891797458876 1101 64-65 0.24328456290155032 1101 66-67 0.5464906070253797 1101 68-69 0.45409459079219516 1101 70-71 0.34305664777758693 1101 72-73 0.4182151987763092 1101 74-75 0.681749424470258 1101 76-77 0.6308933788133579 1101 78-79 0.8561094481239095 1101 80-81 0.8179776845904811 1101 82-83 0.5564253007422515 1101 84-85 0.761065454708536 1101 86-87 0.8498898947391282 1101 88-89 0.7230293060097317 1101 90-91 0.956210872398664 1101 92-93 1.1555126786965424 1101 94-95 1.3585159000857594 1101 96-97 1.3108907910078322 1101 98-99 1.5120943404313287 1101 100-101 1.6488958492628285 1102 1 0.025110995205007924 1102 2 0.033507138001283465 1102 3 -0.08672979334420816 1102 4 0.024007349795020616 1102 5 -0.03450647525973949 1102 6 -0.034739166314153636 1102 7 -0.1828759547227108 1102 8 -0.13607716752858323 1102 9 -0.05248572758649317 1102 10-11 0.018711247678886878 1102 12-13 0.03937547180892409 1102 14-15 0.20876813737074684 1102 16-17 0.31583354415678144 1102 18-19 0.2272548167105768 1102 20-21 0.14311382378641468 1102 22-23 0.1913093111323647 1102 24-25 0.18376232873523435 1102 26-27 0.22700867949264847 1102 28-29 0.1968204347955762 1102 30-31 0.2614810076964247 1102 32-33 0.16405186080803702 1102 34-35 0.25172573601645354 1102 36-37 0.41999033721655366 1102 38-39 0.24135520600567872 1102 40-41 0.42625250363003886 1102 42-43 0.38771473024082326 1102 44-45 0.3162066904866663 1102 46-47 0.32451737500473143 1102 48-49 0.39340823583016515 1102 50-51 0.20144851879236114 1102 52-53 0.17963484870911373 1102 54-55 0.3044159141827407 1102 56-57 0.36746387721198914 1102 58-59 0.35655291541634426 1102 60-61 0.43301781938493633 1102 62-63 0.30056641583704646 1102 64-65 0.13118112040198326 1102 66-67 0.2981864235524405 1102 68-69 0.29983610830062446 1102 70-71 0.3404823827982639 1102 72-73 0.47990461081758795 1102 74-75 0.4611702953267063 1102 76-77 0.6303762992208952 1102 78-79 0.6221233163685973 1102 80-81 0.7509498346137278 1102 82-83 0.6845779306592057 1102 84-85 0.7079629383178094 1102 86-87 0.8053314249598706 1102 88-89 0.6795029241627404 1102 90-91 0.7204277563699364 1102 92-93 0.9715056143441849 1102 94-95 1.154151097606885 1102 96-97 1.0768894700664582 1102 98-99 1.0992472578208812 1102 100-101 1.142517642499861 1103 1 0.23941725377379441 1103 2 0.23277914222872553 1103 3 0.1095301762675902 1103 4 0.1253726691034558 1103 5 0.16340197802793455 1103 6 0.0847786307561833 1103 7 0.12506733196036635 1103 8 0.0863529246970387 1103 9 0.23182963981463445 1103 10-11 0.19151350349317653 1103 12-13 0.12495311636958917 1103 14-15 0.16277637900896735 1103 16-17 0.22748905276387887 1103 18-19 0.1895324356819117 1103 20-21 0.12433968642091742 1103 22-23 0.07092999778645037 1103 24-25 0.15705450068724502 1103 26-27 0.16803380107666044 1103 28-29 -0.03246377637677256 1103 30-31 0.09592065500742564 1103 32-33 0.10921622200739023 1103 34-35 0.21832736312367018 1103 36-37 0.23982945727630778 1103 38-39 0.3778673293864898 1103 40-41 0.3834316730099445 1103 42-43 0.1597501208457217 1103 44-45 0.37740464236900806 1103 46-47 0.21081118287113298 1103 48-49 0.2170543329018173 1103 50-51 0.3521972235489912 1103 52-53 0.38347301488999364 1103 54-55 0.4715677986742861 1103 56-57 0.41518378252451527 1103 58-59 0.39782625008115957 1103 60-61 0.24373800832992032 1103 62-63 0.22303610565131038 1103 64-65 0.1427351991963306 1103 66-67 0.4014561632277669 1103 68-69 0.5597537959401429 1103 70-71 0.6106138298023396 1103 72-73 0.5455943924899351 1103 74-75 0.5541434483696506 1103 76-77 0.41942591028381315 1103 78-79 0.5376957615786395 1103 80-81 0.45908197266853534 1103 82-83 0.75609773777526 1103 84-85 0.7429034327120796 1103 86-87 0.9091687763042096 1103 88-89 0.9536209589573019 1103 90-91 0.9190291241164168 1103 92-93 0.9359264267951666 1103 94-95 1.0711398414201945 1103 96-97 1.1225236972234676 1103 98-99 1.1106739967340786 1103 100-101 1.080889040835654 1104 1 0.04227554735288308 1104 2 0.0779269884136724 1104 3 -0.01836820153700458 1104 4 0.027875953300615208 1104 5 0.048318481960983206 1104 6 0.059009753482690996 1104 7 -0.014571746755464687 1104 8 -0.07792588227970043 1104 9 -0.06223313611176451 1104 10-11 0.04339625504947975 1104 12-13 0.05972302978905475 1104 14-15 0.23565833235426226 1104 16-17 0.11591292642840756 1104 18-19 0.12518705033495792 1104 20-21 0.19112216836391127 1104 22-23 0.2493853431256028 1104 24-25 0.17271001324083812 1104 26-27 0.09348031121338352 1104 28-29 0.08135306337455717 1104 30-31 0.08396073263327963 1104 32-33 0.040715007323370855 1104 34-35 0.23590298475915006 1104 36-37 0.25226789811802774 1104 38-39 0.2105675733653456 1104 40-41 0.23503376551766308 1104 42-43 0.16434013806735237 1104 44-45 0.1900893024763164 1104 46-47 0.13445342525356807 1104 48-49 0.2254777081186532 1104 50-51 0.041604871438593705 1104 52-53 0.03070425953645639 1104 54-55 0.1584547253812829 1104 56-57 0.2324172120044139 1104 58-59 0.3286894277529271 1104 60-61 0.3071214069998476 1104 62-63 0.3619454882083559 1104 64-65 0.29398563425259283 1104 66-67 0.47220686192290984 1104 68-69 0.3800872834970157 1104 70-71 0.3301532485046508 1104 72-73 0.5248621815149157 1104 74-75 0.5146081666095021 1104 76-77 0.4978328176225588 1104 78-79 0.3577657839271602 1104 80-81 0.36772814705645374 1104 82-83 0.5341946002294016 1104 84-85 0.5421378639502628 1104 86-87 0.6376477761993087 1104 88-89 0.6358260811238772 1104 90-91 0.709924108655855 1104 92-93 0.8403754371594054 1104 94-95 0.8149449789396535 1104 96-97 0.8798639907622636 1104 98-99 1.0583305197297115 1104 100-101 0.8463988399351834 1105 1 0.06364173589972211 1105 2 -0.06007043728561001 1105 3 0.08394227403675103 1105 4 0.10707983597528425 1105 5 0.06238081615168767 1105 6 0.1411752280210763 1105 7 0.1226197774211002 1105 8 0.129896891472022 1105 9 0.06667068996957681 1105 10-11 0.09016872530649067 1105 12-13 0.1321678202419747 1105 14-15 0.14080975076981161 1105 16-17 0.1609018627792409 1105 18-19 0.17759566970194385 1105 20-21 0.2021899292673126 1105 22-23 0.20538278754643358 1105 24-25 0.21524235843788375 1105 26-27 0.17918433381851884 1105 28-29 0.1118857693551405 1105 30-31 0.24778274422541102 1105 32-33 0.27863131796708274 1105 34-35 0.3846402380294478 1105 36-37 0.3840186684354876 1105 38-39 0.2831575433207547 1105 40-41 0.23384066033238327 1105 42-43 0.22476013768767444 1105 44-45 0.19985889005192092 1105 46-47 0.2158351754333694 1105 48-49 0.2531691125735307 1105 50-51 0.2899472487098649 1105 52-53 0.34116408619414784 1105 54-55 0.4642206043401629 1105 56-57 0.46659109339393723 1105 58-59 0.374069417057143 1105 60-61 0.4980533176819577 1105 62-63 0.4227083809049148 1105 64-65 0.40676849916951596 1105 66-67 0.434069007095939 1105 68-69 0.35896766827212545 1105 70-71 0.3843197252583934 1105 72-73 0.46742721943874344 1105 74-75 0.42655948847783876 1105 76-77 0.43218867423543017 1105 78-79 0.5173582997510238 1105 80-81 0.5434887778572346 1105 82-83 0.6091227519040565 1105 84-85 0.6781854051413951 1105 86-87 0.8512591397257232 1105 88-89 0.858196243451097 1105 90-91 0.9447903812309626 1105 92-93 1.099132901831009 1105 94-95 1.1656663754063707 1105 96-97 1.1977148494646883 1105 98-99 1.1688620744224032 1105 100-101 1.0960447707929966 1106 1 -0.11941509250097582 1106 2 -0.9730956841220824 1106 3 -0.3886777336054337 1106 4 -0.06263990066086933 1106 5 -0.2084075988773293 1106 6 -0.16744910558950465 1106 7 -0.13178800303025184 1106 8 -0.15277115222041004 1106 9 -0.11804530230229915 1106 10-11 -0.6551268416117466 1106 12-13 -0.501134856748294 1106 14-15 -0.4265284301597134 1106 16-17 -0.32823367822000904 1106 18-19 -0.22099291405365307 1106 20-21 -0.2647426537608979 1106 22-23 -0.20280662817928174 1106 24-25 -0.20274776771956482 1106 26-27 -0.2616401801814163 1106 28-29 -0.05013600895701131 1106 30-31 -0.14729027087788182 1106 32-33 -0.09521952474506179 1106 34-35 -0.07026045767035782 1106 36-37 -0.29669970664853196 1106 38-39 -0.14019256739886998 1106 40-41 0.02884611574835816 1106 42-43 -0.03318517502813023 1106 44-45 -0.0821034886782499 1106 46-47 -0.04050756088359009 1106 48-49 0.0287382578609936 1106 50-51 0.029164409944641534 1106 52-53 0.07690222085621912 1106 54-55 0.06699069538652935 1106 56-57 -0.07663833063382697 1106 58-59 -0.24371726275060723 1106 60-61 -0.04181437888949091 1106 62-63 -0.09074265309207874 1106 64-65 -0.05174161406681321 1106 66-67 0.16918208996608897 1106 68-69 0.22030961044624675 1106 70-71 0.185721675090047 1106 72-73 0.31807777395760795 1106 74-75 -0.06570533974177906 1106 76-77 0.18026527564171246 1106 78-79 0.30985697809785506 1106 80-81 0.21518749349425548 1106 82-83 0.004803746646597773 1106 84-85 0.01389442123295126 1106 86-87 0.028464248020714678 1106 88-89 0.31774655911328153 1106 90-91 0.2447569274846053 1106 92-93 0.33810366778368817 1106 94-95 0.3611088587485547 1106 96-97 0.5202850697223091 1106 98-99 0.3067700125513859 1106 100-101 0.05552521531348731 1107 1 0.07563326798067038 1107 2 0.11342424006959462 1107 3 -0.015141720567708461 1107 4 0.028015477808914113 1107 5 -0.03436143177226114 1107 6 0.0850180346068754 1107 7 0.06285895320024792 1107 8 -0.03286738304368697 1107 9 0.12356870835007783 1107 10-11 0.00686668037103999 1107 12-13 0.03412750090687666 1107 14-15 -0.05621534301281628 1107 16-17 0.08948462810116098 1107 18-19 0.13034521924809184 1107 20-21 0.07642543158882376 1107 22-23 0.17967250002501345 1107 24-25 -0.013237223582457602 1107 26-27 0.004618427902627786 1107 28-29 0.08331762992416003 1107 30-31 -0.044529557234014305 1107 32-33 -0.16334852755520046 1107 34-35 -0.03092534281421422 1107 36-37 -0.00715720129783648 1107 38-39 0.1781930441324988 1107 40-41 0.17753560387880185 1107 42-43 0.08050346556853327 1107 44-45 0.01752323906531217 1107 46-47 0.08399673396830565 1107 48-49 -0.05602476965219694 1107 50-51 -0.11533831237470338 1107 52-53 -0.02590883425185808 1107 54-55 0.1116369878857526 1107 56-57 0.20083917398165596 1107 58-59 0.20678324168516582 1107 60-61 0.18451941463084864 1107 62-63 0.12654305360813822 1107 64-65 0.019297084529760866 1107 66-67 0.32067211561674824 1107 68-69 0.42244922478774427 1107 70-71 0.2986909841560035 1107 72-73 0.29647173829175344 1107 74-75 0.315722080733007 1107 76-77 0.47070652419171566 1107 78-79 0.4390485431966198 1107 80-81 0.4585956718888795 1107 82-83 0.5439737966408806 1107 84-85 0.38218940791748324 1107 86-87 0.41244320467600204 1107 88-89 0.5142835156705381 1107 90-91 0.6338480445137016 1107 92-93 0.6658562634835263 1107 94-95 0.7439620250530616 1107 96-97 0.49958691632482655 1107 98-99 0.8061213428730802 1107 100-101 0.75721175647708 1108 1 0.07700188342076686 1108 2 0.06067174093229255 1108 3 0.07724642267805848 1108 4 0.09902569975946562 1108 5 0.01623955795427179 1108 6 0.12953297361703164 1108 7 0.14735794238259103 1108 8 0.036815010375029544 1108 9 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2316 18-19 -0.1828299400211506 2316 20-21 -2.5308876215035525E-4 2316 22-23 -0.17745060376618227 2316 24-25 -0.09392637710992346 2316 26-27 -0.11997439452753866 2316 28-29 0.13266474023721742 2316 30-31 0.17547757606019587 2316 32-33 0.49298973817582237 2316 34-35 0.10372683002936611 2316 36-37 0.11197724548532761 2316 38-39 0.1869987971518441 2316 40-41 0.04618301885028586 2316 42-43 -0.04158110789209246 2316 44-45 -0.2153751273048954 2316 46-47 -0.269267890163313 2316 48-49 -0.05674653252462747 2316 50-51 -0.052055802298482945 2316 52-53 -0.032608859903611176 2316 54-55 -0.08088623045981791 2316 56-57 -0.03542800478291497 2316 58-59 0.08939522210550166 2316 60-61 0.05881109257140338 2316 62-63 0.26149935778867217 2316 64-65 0.5917442963956248 2316 66-67 0.5890623103240493 2316 68-69 0.6473113905683157 2316 70-71 0.7579509136664058 2316 72-73 0.8099982913645185 2316 74-75 0.7158143216064303 2316 76-77 0.800740679669417 2316 78-79 0.8686185074598676 2316 80-81 0.9416869534572214 2316 82-83 0.9840116066141391 2316 84-85 1.293894335512789 2316 86-87 1.3655616489853557 2316 88-89 1.608056466078331 2316 90-91 1.7847310971419503 2316 92-93 1.7933580995161513 2316 94-95 2.1752554002256836 2316 96-97 2.315228508700983 2316 98-99 2.636049377422122 2316 100-101 3.503833456450021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11050.0 3 2255.0 4 2165.0 5 2454.0 6 2907.0 7 3458.0 8 4240.0 9 5464.0 10 6927.0 11 8754.0 12 11198.0 13 13919.0 14 15685.0 15 19218.0 16 23343.0 17 29538.0 18 26198.0 19 20663.0 20 20856.0 21 22477.0 22 24808.0 23 27792.0 24 30946.0 25 35637.0 26 41104.0 27 47512.0 28 55594.0 29 65735.0 30 78416.0 31 94164.0 32 115756.0 33 144437.0 34 186965.0 35 247710.0 36 328127.0 37 393339.0 38 254819.0 39 22667.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.868246688620296 19.411791941240654 15.33603021225527 22.383931157883783 2 14.843456589894968 20.48446647867942 40.96708994513967 23.70498698628594 3 18.884883026122385 25.11517594920586 30.093553709937222 25.906387314734538 4 12.353552359933047 17.06952779223694 38.31963478547906 32.25728506235095 5 13.4174186522458 37.523892683913765 34.50846907845401 14.55021958538642 6 30.75988359620263 36.73851560593679 18.153784679177182 14.347816118683399 7 27.71188761830444 33.09592951251836 21.197379933044918 17.994802936132288 8 25.904421964229275 34.717971015916845 20.293198124501526 19.084408895352357 9 26.015436901154697 17.166693964458275 21.00581442961264 35.81205470477438 10-11 24.2195656344549 27.139257373450643 27.76412902179608 20.877047970298378 12-13 25.36123331873598 24.468317498378013 28.300214947567248 21.870234235318758 14-15 22.529645482465934 25.809108210744903 26.920618049992868 24.740628256796295 16-17 21.447900909070437 29.14318934088811 27.404308105485743 22.00460164455571 18-19 21.291974633469017 27.63303429033642 30.029342127238728 21.045648948955833 20-21 22.307824093850893 26.575993590878543 30.090346260810225 21.02583605446034 22-23 21.85477309639527 26.534320302353926 30.005986902499455 21.604919698751345 24-25 21.621224420954725 26.76356072673206 30.133811151352297 21.481403700960914 26-27 21.41219727255683 26.932170877417338 30.04098860604354 21.614643243982293 28-29 21.297783666147456 27.15484408364533 30.055257372682142 21.492114877525072 30-31 21.546239993448395 26.829412607743176 30.006285445201975 21.618061953606453 32-33 21.2691400232357 26.86564750318595 30.010266698739436 21.85494577483891 34-35 21.744077428298922 26.967699381083758 29.84968358155424 21.43853960906308 36-37 21.734936059810558 26.668856854828395 29.83846850581065 21.7577385795504 38-39 21.40310956629518 27.128158936754033 29.866298104857965 21.602433392092827 40-41 21.795561340602845 26.976644235964436 29.69980516397335 21.52798925945937 42-43 21.4563545777223 26.924121607003194 29.567759230758682 22.05176458451582 44-45 21.69359559530876 27.12059684385042 29.365638496018992 21.820169064821826 46-47 21.81674070456127 27.326543859494695 28.923355068083406 21.933360367860626 48-49 21.836504118749023 27.379798426033762 28.863444723151567 21.92025273206565 50-51 21.877906685107025 27.575116983998456 28.66143279933418 21.88554353156034 52-53 21.96565147323522 27.645710939147776 28.42247418331637 21.96616340430063 54-55 21.77327100109892 27.547785043866764 28.593561404456935 22.085382550577386 56-57 21.937744264141738 27.80988697743642 28.347104359628855 21.905264398792983 58-59 22.094319865215823 27.421757379885964 28.518055501269075 21.96586725362914 60-61 21.917254550127947 27.48709903455592 28.764405123058513 21.83124129225762 62-63 21.68915530978251 27.485740365153077 28.939517045047552 21.885587280016864 64-65 21.73176866967299 27.46830712963729 28.687562773861845 22.11236142682788 66-67 21.66667519377862 27.638013865084034 28.441615717172752 22.253695223964595 68-69 21.696889919251852 27.519532145359765 28.656874398318237 22.126703537070146 70-71 21.845742893361432 27.4114926795964 28.559192960755237 22.18357146628693 72-73 21.83428974785981 27.33238220588028 28.55283658993179 22.280491456328125 74-75 21.70828345411115 27.739042142919857 28.283130736195826 22.26954366677316 76-77 21.793155538634423 27.521579560364433 28.33477640566503 22.350488495336112 78-79 21.804339829766207 27.49885935621635 28.285345782401805 22.41145503161564 80-81 21.73922209382244 27.77682262532385 28.19630511553729 22.28765016531642 82-83 21.84396713158622 27.57506421210974 28.274183150333084 22.306785505970957 84-85 21.88875005678565 27.555610033400196 28.22162896849839 22.334010941315764 86-87 21.837883190766586 27.477468996848526 28.30217329022224 22.38247452216265 88-89 21.970782139861047 27.24590733631034 28.178160139710005 22.60515038411861 90-91 21.933753647527947 27.204592702774722 28.379947058912613 22.481706590784714 92-93 21.906879531851594 27.066221419197657 28.492815759075164 22.534083289875586 94-95 21.793831974506155 27.17484490500694 28.358321058966286 22.673002061520624 96-97 21.75226697406038 27.193332198072458 28.43902175443652 22.61537907343064 98-99 21.743451882772394 27.235934854812292 28.13559162451764 22.88502163789767 100-101 22.258451963394794 26.434504356880485 28.66127252139557 22.645771158329154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3743.0 1 2082.5 2 421.5 3 412.0 4 356.5 5 360.0 6 579.0 7 1060.5 8 1888.5 9 2478.5 10 2473.5 11 2378.0 12 2456.5 13 2653.0 14 2917.0 15 3201.5 16 3392.0 17 3542.5 18 3733.0 19 4088.0 20 4601.0 21 5358.0 22 6418.0 23 7621.0 24 9127.0 25 11637.5 26 15006.0 27 18597.0 28 21699.5 29 25370.0 30 30336.5 31 35467.0 32 40899.0 33 46632.5 34 53024.0 35 58932.5 36 65280.5 37 71263.0 38 74870.5 39 77698.5 40 79665.0 41 83187.0 42 86518.5 43 89506.0 44 93653.0 45 95503.0 46 95918.0 47 97526.5 48 100006.0 49 98209.5 50 92081.0 51 85469.0 52 79480.0 53 75734.0 54 72799.0 55 66302.0 56 58055.5 57 51001.0 58 45229.0 59 39576.5 60 32588.0 61 26301.5 62 20816.5 63 15663.0 64 11723.5 65 8911.0 66 6575.5 67 4934.5 68 3808.5 69 2728.5 70 1889.0 71 1282.5 72 896.0 73 686.0 74 535.0 75 405.5 76 296.5 77 207.0 78 133.0 79 86.0 80 66.0 81 49.5 82 31.0 83 19.5 84 16.0 85 10.5 86 4.0 87 3.0 88 4.5 89 6.0 90 6.0 91 4.5 92 2.0 93 0.5 94 0.5 95 1.5 96 1.5 97 0.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3145855023544899 2 0.271453550850161 3 0.2696563862041473 4 0.2678592215581336 5 0.2717803080585271 6 0.2356327918830242 7 0.2355919472319784 8 0.2392271211750516 9 0.24919321603021852 10-11 0.25648398624188773 12-13 0.248233366730643 14-15 0.237409534203515 16-17 0.23265113235668325 18-19 0.22043858169399924 20-21 0.22597303191070053 22-23 0.2233589742437715 24-25 0.22587092028308614 26-27 0.22166392122537223 28-29 0.23777713606292689 30-31 0.2512354485825068 32-33 0.2425559602352815 34-35 0.24043203838090169 36-37 0.2282603323692634 38-39 0.21612947100867091 40-41 0.21324992310994442 42-43 0.225891342608609 44-45 0.22264419285047057 46-47 0.23467294258344867 48-49 0.23986021326626097 50-51 0.25297134625195183 52-53 0.2681859787664997 54-55 0.24118766442524836 56-57 0.21439357333922585 58-59 0.203712697090758 60-61 0.20720491475517103 62-63 0.21304569985471558 64-65 0.2045091677861505 66-67 0.20885912312252455 68-69 0.21030910823464927 70-71 0.2123104961358918 72-73 0.213842170550108 74-75 0.21715058728481507 76-77 0.22856666725210667 78-79 0.2287913128328584 80-81 0.2209695621575942 82-83 0.21345414636517324 84-85 0.20022047942634508 86-87 0.20426409987987587 88-89 0.21404639380533685 90-91 0.21271894264634944 92-93 0.2170893203082464 94-95 0.21245345241455194 96-97 0.22043858169399924 98-99 0.22384911005632072 100-101 0.20816476405474652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2448301.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.303526099788385 #Duplication Level Percentage of deduplicated Percentage of total 1 78.43186183491026 45.72854103546687 2 12.469398933446412 14.540198523297324 3 3.9025881904898676 6.826039572628558 4 1.6873746057615275 3.935195574685494 5 0.9169787141092338 2.673154619550905 6 0.5780103571573768 2.0220025166683873 7 0.3584221878677057 1.4628094169561623 8 0.2654830233042478 1.238287710261595 9 0.1916343109448558 1.0055660444809527 >10 1.0781244030728083 11.672865980471773 >50 0.07974557633654568 3.181762202960097 >100 0.03862550466388216 3.894402748284087 >500 0.0011215090786469235 0.41607159650299214 >1k 4.906602219067841E-4 0.6835082458990333 >5k 7.009431741525488E-5 0.3050755788123338 >10k+ 7.009431741525488E-5 0.4145186330734765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10143 0.4142872955572047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7465 0.30490532005664334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4858 0.19842331478033137 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4093 0.16717715673032035 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.084465104576602E-5 10-11 0.0 0.0 0.0 0.0 4.084465104576602E-5 12-13 0.0 0.0 0.0 0.0 4.084465104576602E-5 14-15 0.0 2.042232552288301E-5 0.0 0.0 4.084465104576602E-5 16-17 0.0 4.084465104576602E-5 0.0 0.0 4.084465104576602E-5 18-19 2.042232552288301E-5 4.084465104576602E-5 0.0 2.042232552288301E-5 4.084465104576602E-5 20-21 4.084465104576602E-5 8.168930209153204E-5 0.0 4.084465104576602E-5 4.084465104576602E-5 22-23 4.084465104576602E-5 8.168930209153204E-5 0.0 1.0211162761441505E-4 4.084465104576602E-5 24-25 4.084465104576602E-5 8.168930209153204E-5 0.0 2.8591255732036215E-4 6.126697656864903E-5 26-27 4.084465104576602E-5 8.168930209153204E-5 0.0 4.697134870263093E-4 8.168930209153204E-5 28-29 4.084465104576602E-5 8.168930209153204E-5 0.0 0.0010619609271899167 8.168930209153204E-5 30-31 4.084465104576602E-5 8.168930209153204E-5 0.0 0.0027161692945434405 8.168930209153204E-5 32-33 4.084465104576602E-5 8.168930209153204E-5 0.0 0.004227421383236784 8.168930209153204E-5 34-35 4.084465104576602E-5 8.168930209153204E-5 0.0 0.006147119982387787 8.168930209153204E-5 36-37 4.084465104576602E-5 8.168930209153204E-5 0.0 0.008046396256015907 8.168930209153204E-5 38-39 4.084465104576602E-5 8.168930209153204E-5 0.0 0.010149895784872857 8.168930209153204E-5 40-41 4.084465104576602E-5 8.168930209153204E-5 0.0 0.013029443683599361 8.168930209153204E-5 42-43 4.084465104576602E-5 8.168930209153204E-5 0.0 0.018808961806575254 8.168930209153204E-5 44-45 4.084465104576602E-5 8.168930209153204E-5 0.0 0.02712084829438864 8.168930209153204E-5 46-47 6.126697656864903E-5 8.168930209153204E-5 0.0 0.0463995235879902 1.2253395313729806E-4 48-49 8.168930209153204E-5 8.168930209153204E-5 0.0 0.08217943790408125 1.633786041830641E-4 50-51 8.168930209153204E-5 8.168930209153204E-5 0.0 0.11536571687876614 1.633786041830641E-4 52-53 8.168930209153204E-5 1.0211162761441505E-4 0.0 0.14875621910867987 2.042232552288301E-4 54-55 8.168930209153204E-5 1.2253395313729806E-4 0.0 0.19215366084480626 2.4506790627459613E-4 56-57 8.168930209153204E-5 1.2253395313729806E-4 0.0 0.22758639562700828 2.4506790627459613E-4 58-59 8.168930209153204E-5 2.042232552288301E-4 0.0 0.2750274578166655 2.4506790627459613E-4 60-61 8.168930209153204E-5 2.042232552288301E-4 0.0 0.35085555248313016 2.4506790627459613E-4 62-63 8.168930209153204E-5 2.042232552288301E-4 0.0 0.4077521513898822 2.4506790627459613E-4 64-65 8.168930209153204E-5 2.6549023179747917E-4 0.0 0.47769861630575655 2.4506790627459613E-4 66-67 8.168930209153204E-5 3.0633488284324514E-4 0.0 0.5979452689844917 2.4506790627459613E-4 68-69 8.168930209153204E-5 3.267572083661282E-4 0.0 0.7394515625325481 2.4506790627459613E-4 70-71 1.0211162761441505E-4 3.267572083661282E-4 0.0 0.8708896495978231 2.4506790627459613E-4 72-73 1.2253395313729806E-4 3.267572083661282E-4 0.0 1.011640317101533 2.4506790627459613E-4 74-75 1.2253395313729806E-4 3.267572083661282E-4 0.0 1.1459987967165803 2.4506790627459613E-4 76-77 1.2253395313729806E-4 3.267572083661282E-4 0.0 1.26755247822878 2.4506790627459613E-4 78-79 1.2253395313729806E-4 3.267572083661282E-4 0.0 1.416084051756708 2.4506790627459613E-4 80-81 2.042232552288301E-4 3.267572083661282E-4 0.0 1.5894491731204619 2.4506790627459613E-4 82-83 2.042232552288301E-4 3.267572083661282E-4 0.0 1.8021272711157656 2.4506790627459613E-4 84-85 2.042232552288301E-4 3.267572083661282E-4 0.0 2.0467458862288583 2.4506790627459613E-4 86-87 2.042232552288301E-4 3.267572083661282E-4 0.0 2.4190653028365383 2.4506790627459613E-4 88-89 2.042232552288301E-4 3.267572083661282E-4 0.0 2.9032786409840945 2.4506790627459613E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7720 0.0 35.958515 1 GTATCAA 12260 0.0 34.97214 1 ATCAACG 15070 0.0 28.092648 3 TCAACGC 15265 0.0 27.734352 4 TATCAAC 15340 0.0 27.660706 2 CAACGCA 15375 0.0 27.47637 5 AACGCAG 15870 0.0 26.609001 6 ACGCAGA 17680 0.0 23.510193 7 CGCAGAG 17810 0.0 23.28524 8 GTACATG 10325 0.0 22.696718 1 GTACCGG 210 3.4240657E-8 22.635332 1 TACATGG 10350 0.0 22.173994 2 GCAGAGT 18310 0.0 21.896545 9 GAGTACT 8975 0.0 21.51443 12-13 AGAGTAC 14575 0.0 21.445631 10-11 AGTACTT 9265 0.0 20.994823 12-13 GTACTTT 9265 0.0 20.711357 14-15 TGCGCCG 115 0.0037155726 20.660692 5 ACATGGG 10800 0.0 20.589714 3 CAGAGTA 17975 0.0 18.010197 10-11 >>END_MODULE